{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:53:56Z","timestamp":1789480436675,"version":"build-2803163510"},"reference-count":34,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["408490\/2022-5"],"award-info":[{"award-number":["408490\/2022-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz\/Ministry of Health\/PAHO","award":["BIO-006-FIO-21"],"award-info":[{"award-number":["BIO-006-FIO-21"]}]},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz\/Ministry of Health\/PAHO","award":["VPGDI-PT69"],"award-info":[{"award-number":["VPGDI-PT69"]}]},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz\/Ministry of Health\/PAHO","award":["BIO-003-FEX-23"],"award-info":[{"award-number":["BIO-003-FEX-23"]}]},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz\/Ministry of Health\/PAHO","award":["BIO-002-AEP-23"],"award-info":[{"award-number":["BIO-002-AEP-23"]}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"crossref","award":["E-26\/200.205\/2023"],"award-info":[{"award-number":["E-26\/200.205\/2023"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Viruses"],"abstract":"<jats:p>Background: CLEC5A is a C-type lectin expressed by monocytes and neutrophils, playing an important role in innate immunity. Although it has been shown to interact with the spike protein of SARS-CoV-2, its role during vaccination remains poorly understood. Methods: To address this question, we combined in vitro assays to characterize CLEC5A and spike expression and their impact on monocyte differentiation and T-cell activation; in vivo studies to evaluate CLEC5A expression, immune responses, and vaccine efficacy in a murine model; and in silico analyses to identify potential spike epitopes and CLEC5A interaction sites. Results: The Pfizer-BioNTech bivalent mRNA vaccine induced spike expression and CLEC5A upregulation in THP-1 monocytes, promoting M1-like differentiation and CD86+ activation. In PBMC co-cultures, CLEC5A+ monocytes acted as antigen-presenting cells, releasing inflammatory chemokines and activating both CD4+ and CD8+ T cells, thereby linking CLEC5A expression to adaptive immunity. In mice, CLEC5A expression was observed on inflammatory monocytes (CCR2+CX3CR1low) within two days of vaccination. In vivo, CLEC5A expression increased during SARS-CoV-2 infection and after immunization, but declined following viral challenge in vaccinated animals. Consistently, robust humoral and cellular responses were detected post-immunization. In silico analysis further suggested differential CLEC5A binding across B- and T-cell epitopes within the spike glycoprotein. Conclusions: These findings suggest that CLEC5A may play a role in bridging innate and adaptive immune responses during SARS-CoV-2 vaccination. Although further studies with different vaccine platforms are necessary to confirm and expand these observations, our results provide preliminary evidence supporting the potential of CLEC5A as an exploratory biomarker of vaccine-induced immunity.<\/jats:p>","DOI":"10.3390\/v17091233","type":"journal-article","created":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T12:04:55Z","timestamp":1757505895000},"page":"1233","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["CLEC5A Activation in Inflammatory Monocytes: A Mechanism for Enhanced Adaptive Immunity Following COVID-19 mRNA Vaccination in a Preclinical Study"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7182-9918","authenticated-orcid":false,"given":"Renan","family":"Galuzo","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio-Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago","family":"Lazari Machado","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio-Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryann","family":"de Souza Nascimento","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio-Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorvan","family":"Ramos de Medeiros","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia em Imunobiol\u00f3gicos, Bio-Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Neves 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China, 2019","volume":"382","author":"Zhu","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2012-7","article-title":"A pneumonia outbreak associated with a new coronavirus of probable bat origin","volume":"579","author":"Zhou","year":"2020","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1016\/j.ymthe.2022.02.016","article-title":"COVID-19 mRNA vaccines: Platforms and current developments","volume":"30","author":"Mahiny","year":"2022","journal-title":"Mol. Ther."},{"key":"ref_4","first-page":"8827670","article-title":"Cellular and molecular immunology approaches for the development of immunotherapies against the new coronavirus (SARS-CoV-2): Challenges to near-future breakthroughs","volume":"2020","author":"Azamor","year":"2020","journal-title":"J. Immunol. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/s41577-021-00578-z","article-title":"Immunological mechanisms of vaccine-induced protection against COVID-19 in humans","volume":"21","author":"Sadarangani","year":"2021","journal-title":"Nat. Rev. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mason, T.F.D., Whitston, M., Hodgson, J., Watkinson, R.E., Lau, Y.S., Abdulrazeg, O., and Sutton, M. (2021). Effects of BNT162b2 mRNA vaccine on COVID-19 infection and hospitalisation amongst older people: Matched case control study for England. BMC Med., 19.","DOI":"10.1186\/s12916-021-02149-4"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1038\/s41418-022-01015-x","article-title":"Immune response in COVID-19: What is next?","volume":"29","author":"Li","year":"2022","journal-title":"Cell Death Differ."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1056\/NEJMoa2102214","article-title":"Efficacy of the ChAdOx1 nCoV-19 Covid-19 vaccine against the B.1.351 variant","volume":"384","author":"Madhi","year":"2021","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Azamor, T., da Silva, A.M.V., Melga\u00e7o, J.G., Dos Santos, A.P., Xavier-Carvalho, C., Alvarado-Arnez, L.E., and da Costa Neves, P.C. (2021). Activation of an Effective Immune Response after Yellow Fever Vaccination Is Associated with the Genetic Background and Early Response of IFN-\u03b3 and CLEC5A. Viruses, 13.","DOI":"10.3390\/v13010096"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/s41467-017-00356-3","article-title":"CLEC5A is a critical receptor in innate immunity against Listeria infection","volume":"8","author":"Chen","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e28427","DOI":"10.1002\/jmv.28427","article-title":"CLEC5A expression can be triggered by spike glycoprotein and may be a potential target for COVID-19 therapy","volume":"95","author":"Machado","year":"2023","journal-title":"J. Med. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/s41541-025-01124-x","article-title":"Ionizable lipid nanoparticles of mRNA vaccines elicit NF-kB and IRF responses through toll-like receptor 4","volume":"10","author":"Zelkoski","year":"2025","journal-title":"npj Vaccines"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lou, B., De Koker, S., Lau, C.Y.J., Hennink, W.E., and Mastrobattista, E. (2019). mRNA Polyplexes with Post-Conjugated GALA Peptides Efficiently Target, Transfect, and Activate Antigen Presenting Cells. Bioconjugate Chem., 30.","DOI":"10.1021\/acs.bioconjchem.8b00524"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.ymeth.2025.06.006","article-title":"Inside the cell: Approaches to evaluating mRNA internalization and trafficking","volume":"241","author":"Plank","year":"2025","journal-title":"Methods"},{"key":"ref_15","first-page":"16","article-title":"Efficient in vitro and in vivo transfection of self-amplifying mRNA with linear poly(propylenimine) and poly(ethylenimine-propylenimine) random copolymers as non-viral carriers","volume":"12","author":"Opsomer","year":"2024","journal-title":"J. Mater. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sharma, S., Malmestr\u00f6m, C., Lindberg, C., Meisel, S., Sch\u00f6n, K., Verolin, M., and Lycke, N.Y. (2017). A Sensitive Method for Detecting Peptide-specific CD4+ T Cell Responses in Peripheral Blood from Patients with Myasthenia Gravis. Front. Immunol., 8.","DOI":"10.3389\/fimmu.2017.01370"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Azamor, T., Horbach, I.S., Brito e Cunha, D., Melga\u00e7o, J.G., Silva, A.M.V.D., Tubar\u00e3o, L.N., and Missailidis, S. (2022). Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation. Viruses, 14.","DOI":"10.3390\/v14091916"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"World Medical Association (2013). World Medical Association Declaration of Helsinki: Ethical Principles for Medical Research Involving Human Subjects. JAMA, 310, 2191\u20132194.","DOI":"10.1001\/jama.2013.281053"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Silva, A.M.V., Machado, T.L., Nascimento, R.D.S., Rodrigues, M.P.M.D., Coelho, F.S., Tubar\u00e3o, L.N., and Ano Bom, A.P.D. (2024). Immunomodulatory effect of bovine lactoferrin during SARS-CoV-2 infection. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1456634"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Winger, A., and Caspari, T. (2021). The Spike of Concern\u2014The Novel Variants of SARS-CoV-2. Viruses, 13.","DOI":"10.3390\/v13061002"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wang, R., Sun, C., Ma, J., Yu, C., Kong, D., Chen, M., and Xie, L. (2022). A bivalent COVID-19 vaccine based on alpha and beta variants elicits potent and broad immune responses in mice against SARS-CoV-2 variants. Vaccines, 10.","DOI":"10.3390\/vaccines10050702"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5283","DOI":"10.1016\/j.vaccine.2023.06.086","article-title":"Immunogenicity and protective effects of recombinant bivalent COVID-19 vaccine in mice and rhesus macaques","volume":"41","author":"Liu","year":"2023","journal-title":"Vaccine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/s41586-022-04441-6","article-title":"SARS-CoV-2 Omicron virus causes attenuated disease in mice and hamsters","volume":"603","author":"Halfmann","year":"2022","journal-title":"Nature"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1093\/cid\/ciab817","article-title":"Low environmental temperature exacerbates severe acute respiratory syndrome coronavirus 2 infection in golden Syrian hamsters","volume":"75","author":"Chan","year":"2022","journal-title":"Clin. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jep.2021.114838","article-title":"Inhibitory effects and mechanisms of the anti-COVID-19 traditional Chinese prescription, Keguan-1, on acute lung injury","volume":"285","author":"Bai","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sung, P.S., Yang, S.P., Peng, Y.C., Sun, C.P., Tao, M.H., and Hsieh, S.L. (2022). CLEC5A and TLR2 are critical in SARS-CoV-2-induced NET formation and lung inflammation. J. Biomed. Sci., 29.","DOI":"10.1186\/s12929-022-00832-z"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1126\/sciimmunol.ade9888","article-title":"Exposure to BA.4\/5 S protein drives neutralization of Omicron BA.1, BA.2, BA.2.12.1, and BA.4\/5 in vaccine-experienced humans and mice","volume":"7","author":"Muik","year":"2022","journal-title":"Sci. Immunol."},{"key":"ref_28","first-page":"724","article-title":"A single immunization with nucleoside-modified mRNA vaccines elicits strong cellular and humoral immune responses against SARS-CoV-2 in mice","volume":"53","author":"Hogan","year":"2022","journal-title":"Immunity"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Andries, J., Viranaicken, W., Cordonin, C., Herrscher, C., Planesse, C., Roquebert, B., and Despres, P. (2022). Author Correction: The SARS-CoV-2 spike residues 616\/644 and 1138\/1169 delineate two antibody epitopes in COVID-19 mRNA COMIRNATY vaccine (Pfizer\/BioNTech). Sci. Rep., 12.","DOI":"10.1038\/s41598-022-10885-7"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Nguyen, T.L., Nguyen, T.B., and Kim, H. (2025). Computational identification of B and T-cell epitopes for designing a multiepitope vaccine against SARS-CoV-2 spike glycoprotein. J. Struct. Biol., 217.","DOI":"10.1016\/j.jsb.2025.108177"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"104459","DOI":"10.1016\/j.micpath.2020.104459","article-title":"Identification B and T-Cell epitopes and functional exposed amino acids of S protein as a potential vaccine candidate against SARS-CoV-2\/COVID-19","volume":"148","author":"Tohidinia","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Upadhyay, V., Suryawanshi, R.K., Tasoff, P., McCavitt-Malvido, M., Kumar, R.G., Murray, V.W., and Turnbaugh, P.J. (2023). Mild SARS-CoV-2 infection results in long-lasting microbiota instability. mBio, 14.","DOI":"10.1128\/mbio.00889-23"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Santos, Y.S., Gamon, T.H.M., de Azevedo, M.S.P., Covas, D.T., and Curi, R. (2023). Virulence profiles of wild-type, P.1 and Delta SARS-CoV-2 variants in K18-hACE2 transgenic mice. Viruses, 15.","DOI":"10.3390\/v15040999"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1111\/1751-7915.14064","article-title":"COVID-19: Omicron\u2013the latest, the least virulent, but probably not the last variant of concern of SARS-CoV-2","volume":"15","year":"2022","journal-title":"Microb. Biotechnol."}],"updated-by":[{"DOI":"10.3390\/v17091281","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000}}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/9\/1233\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T08:38:18Z","timestamp":1758530298000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/9\/1233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,10]]},"references-count":34,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["v17091233"],"URL":"https:\/\/doi.org\/10.3390\/v17091233","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,10]]}}}