{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:44:35Z","timestamp":1792809875619,"version":"4.5.0"},"reference-count":86,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Evandro Chagas Institute"},{"name":"Secretariat of Health and Environmental Surveillance"},{"name":"Brazilian Ministry of Health","id":[{"id":"https:\/\/ror.org\/02y7p0749","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Yellow fever (YF) is an acute and potentially fatal hemorrhagic disease caused by the Yellow Fever virus (YFV), endemic to sub-Saharan Africa and several tropical countries, including Brazil. In Brazil, the Amazon region is considered the main endemic area. YFV is maintained in a sylvatic cycle involving Neotropical primates and mosquitoes of the genera Haemagogus and Sabethes, acting as primary and secondary vectors, respectively. In March 2024, entomovirological surveillance was conducted in Santa B\u00e1rbara do Par\u00e1, Par\u00e1, Brazil. A total of 286 mosquitoes were collected, classified into 13 species across nine genera, and grouped into 33 pools. Seventeen pools were tested by RT-qPCR for Orthoflavivirus (YFV, DENV, WNV, SLEV), Alphavirus (CHIKV, MAYV), and Orthobunyavirus (OROV). YFV was detected in four Haemagogus janthinomys pools, with Ct values ranging from 22.2 to 27.9. Metagenomic sequencing confirmed the presence of YFV with assigned reads and &gt;99% protein identity. Notably, the detection occurred without human cases or primate deaths, enabling timely vaccination of the local population. These findings confirm YFV circulation in forested areas of the Bel\u00e9m metropolitan region and reaffirm Hg. janthinomys as a key vector. Our study reinforces the relevance of early entomovirological surveillance and preventive strategies, such as vaccination, to mitigate yellow fever reemergence.<\/jats:p>","DOI":"10.3390\/v17091258","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T12:24:25Z","timestamp":1758198265000},"page":"1258","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Detection of Yellow Fever Virus in Haemagogus janthinomys Mosquitoes (Diptera: Culicidae) in a Rural Settlement in the State of Par\u00e1, Brazilian Amazon, 2024"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5981-0065","authenticated-orcid":false,"given":"Joaquim Pinto","family":"Nunes Neto","sequence":"first","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Graduate Program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3560-5499","authenticated-orcid":false,"given":"Daniel Damous","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Graduate Program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8529-4751","authenticated-orcid":false,"given":"Bruna La\u00eds Sena do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7185-4538","authenticated-orcid":false,"given":"Sandro Patroca","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e2mia Luzia Sena","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3522-3584","authenticated-orcid":false,"given":"L\u00facia Aline Moura","family":"Reis","sequence":"additional","affiliation":[{"name":"Faculty of Nursing, Federal and Rural University of Amazon, Parauapebas 68515-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna Carolina Farias","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7510-6641","authenticated-orcid":false,"given":"F\u00e1bio Silva","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Graduate Program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7774-5893","authenticated-orcid":false,"given":"Lucas Henrique","family":"da Silva e Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Graduate Program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durval Bertram Rodrigues","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Carlos Feitosa","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wallace Oliveira","family":"Ros\u00e1rio","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Amilton dos Santos","family":"Paiva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Wilson Rosa","family":"J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0793-9665","authenticated-orcid":false,"given":"Bruno Tardelli Diniz","family":"Nunes","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9400-2036","authenticated-orcid":false,"given":"L\u00edvia Car\u00edcio","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8578-9984","authenticated-orcid":false,"given":"L\u00edvia Medeiros Neves","family":"Casseb","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana Cec\u00edlia Ribeiro","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Graduate Program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences, State University of Par\u00e1, Bel\u00e9m 66095-663, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jcv.2014.08.030","article-title":"Yellow Fever","volume":"64","author":"Monath","year":"2015","journal-title":"J. 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