{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T22:16:13Z","timestamp":1773785773519,"version":"3.50.1"},"reference-count":70,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Committee of the Ministry of Science","award":["BR21882269"],"award-info":[{"award-number":["BR21882269"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Plant viruses have evolved from being viewed exclusively as pathogens into versatile and powerful tools for modern biotechnology. Among them, Tomato bushy stunt virus (TBSV) holds a special place due to its well-studied molecular biology and unique structural properties. This review systematizes the knowledge on TBSV\u2019s dual role as a multifunctional platform. On one hand, we cover its application as a viral vector for the highly efficient expression of recombinant proteins in plants, as well as a tool for functional genomics, including Virus-Induced Gene Silencing (VIGS) and the delivery of CRISPR\/Cas9 gene-editing components. On the other hand, we provide a detailed analysis of the use of the stable and monodisperse TBSV virion in nanobiotechnology. Its capsid serves as an ideal scaffold for creating next-generation vaccine candidates, platforms for targeted drug delivery to tumor cells, and as a building block for the programmable self-assembly of complex nanoarchitectures. In conclusion, key challenges limiting the widespread adoption of the platform are discussed, including the genetic instability of vectors and difficulties in scalable purification, along with promising strategies to overcome them.<\/jats:p>","DOI":"10.3390\/v17091268","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:08:58Z","timestamp":1758276538000},"page":"1268","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Tomato Bushy Stunt Virus (TBSV): From a Plant Pathogen to a Multifunctional Biotechnology Platform"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7485-6950","authenticated-orcid":false,"given":"Almas","family":"Madirov","sequence":"first","affiliation":[{"name":"Scientific Laboratory for Plant Biotechnology Named After Rustem Omarov, L. N. Gumilyov Eurasian National University, Astana 010008, Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1278-5207","authenticated-orcid":false,"given":"Nurgul","family":"Iksat","sequence":"additional","affiliation":[{"name":"Scientific Laboratory for Plant Biotechnology Named After Rustem Omarov, L. N. Gumilyov Eurasian National University, Astana 010008, Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaksylyk","family":"Masalimov","sequence":"additional","affiliation":[{"name":"Scientific Laboratory for Plant Biotechnology Named After Rustem Omarov, L. N. Gumilyov Eurasian National University, Astana 010008, Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1094\/PHYTO-05-22-0167-RVW","article-title":"Plant Viruses of Agricultural Importance: Current and Future Perspectives of Virus Disease Management Strategies","volume":"113","author":"Tatineni","year":"2023","journal-title":"Phytopathology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Venkataraman, S., and Hefferon, K. (2021). Application of Plant Viruses in Biotechnology, Medicine, and Human Health. Viruses, 13.","DOI":"10.3390\/v13091697"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.copbio.2014.11.011","article-title":"Means to optimize protein expression in transgenic plants","volume":"32","author":"Ullrich","year":"2015","journal-title":"Curr. Opin. 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