{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:47:04Z","timestamp":1760060824191,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000233","name":"Genome BC Genesolve grant","doi-asserted-by":"publisher","award":["Geno24","IT31908"],"award-info":[{"award-number":["Geno24","IT31908"]}],"id":[{"id":"10.13039\/501100000233","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004489","name":"Mitacs Accelerate grant","doi-asserted-by":"publisher","award":["Geno24","IT31908"],"award-info":[{"award-number":["Geno24","IT31908"]}],"id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Phyto Diagnostics Company Limited","award":["Geno24","IT31908"],"award-info":[{"award-number":["Geno24","IT31908"]}]},{"name":"British Columbia Blueberry Council","award":["Geno24","IT31908"],"award-info":[{"award-number":["Geno24","IT31908"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The Fraser Valley of British Columbia, Canada is among the top ten blueberry producing regions globally. Viral diseases are established in the region and significantly reduce average yields. While testing for two viruses is routine, characterization of all the viruses present in the region is incomplete. We used high-throughput sequencing to obtain an unbiased overview of RNA viruses present in 97 plants collected across the region. In addition to known viruses, we identified four luteoviruses previously unidentified in the region. Two of them matched the blueberry virus L (BlVL) and blueberry virus M (BlVM). recently found in the USA, while the third constitutes a new major variant of BlVM (BlVM-2), and the fourth a new luteovirus, which we named blueberry virus N (BlVN). The genome sequences were ~5 kbp long and contained four open-reading frames similar to other luteoviruses. PCR screening revealed that these luteoviruses are widespread in the region, and that plants typically harbour more than one of these luteoviruses. While luteoviruses are typically vectored by aphids, they were also present in nursery stock, indicating that spread also occurs via vegetative propagation.<\/jats:p>","DOI":"10.3390\/v17101286","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T10:27:53Z","timestamp":1758623273000},"page":"1286","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of Novel Luteoviruses in Canadian Highbush Blueberries Using High-Throughput Sequencing"],"prefix":"10.3390","volume":"17","author":[{"given":"Sachithrani","family":"Kannangara","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3339-514X","authenticated-orcid":false,"given":"Adam","family":"Gilewski","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Rodriguez Lopez","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gertruida","family":"de Villiers","sequence":"additional","affiliation":[{"name":"Phyto Diagnostics Company Ltd., 9381 Ardmore Dr, North Saanich, BC V8L 5G4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meghan","family":"Ellis","sequence":"additional","affiliation":[{"name":"Phyto Diagnostics Company Ltd., 9381 Ardmore Dr, North Saanich, BC V8L 5G4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Ellis","sequence":"additional","affiliation":[{"name":"Phyto Diagnostics Company Ltd., 9381 Ardmore Dr, North Saanich, BC V8L 5G4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7110-0979","authenticated-orcid":false,"given":"Eric","family":"Gerbrandt","sequence":"additional","affiliation":[{"name":"British Columbia Blueberry Council, 275-32160 S Fraser Way, Abbotsford, BC V2T 1W5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3445-9704","authenticated-orcid":false,"given":"Jim","family":"Mattsson","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Simon Fraser University, 8888 University Dr, Burnaby, BC V5A 1S6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"ref_1","unstructured":"(2024, January 26). 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