{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:41:14Z","timestamp":1777387274077,"version":"3.51.4"},"reference-count":84,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute for Scientific Research Bento Rocha Cabral and Technology and Foundation for Science and Technology","award":["PTDC\/SAU-GMG\/103307\/2008"],"award-info":[{"award-number":["PTDC\/SAU-GMG\/103307\/2008"]}]},{"name":"PhD scholarship","award":["PDE\/BDE\/114585\/2016"],"award-info":[{"award-number":["PDE\/BDE\/114585\/2016"]}]},{"name":"the Foundation for Science and Technology to ISAMB","award":["UID 04295\u2014Instituto de Sa\u00fade Ambiental"],"award-info":[{"award-number":["UID 04295\u2014Instituto de Sa\u00fade Ambiental"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Viruses"],"abstract":"<jats:p>Chronic hepatitis C (CHC) is linked to iron overload, which significantly correlates with liver fibrosis. This study aimed to assess whether genetic polymorphisms related to iron metabolism are associated with fibrosis severity, predict improvement in fibrosis after HCV clearance with direct-acting antivirals (DAAs) and influence iron-related metabolic markers before treatment. A total of 329 CHC patients were included, 134 of whom received DAAs therapy. Liver fibrosis was assessed using transient elastography (FibroScan), and biochemical parameters were measured using standard methods. Eighteen genetic polymorphisms within five iron metabolism-related genes were analyzed using PCR-RFLP, endpoint genotyping, or next-generation sequencing (NGS). Before DAA treatment, patients with severe fibrosis showed higher levels of serum iron (Fe), total iron-binding capacity (TIBC), and ferritin (Ft). SLC40A1 rs1439816_GG was associated with an increased risk of severe fibrosis compared with GC or CC genotypes. SLC40A1 rs11568351_GC genotype was linked to a higher likelihood of remaining cirrhotic after HCV clearance. Elevated iron parameters were observed in carriers HFE C282Y_CY, TF IVS 11 G&gt;A, and BMP2 570 A&gt;T. Overall, polymorphisms in iron metabolism genes may influence both the severity of liver fibrosis prior to treatment, its regression after DAA therapy and the regulation of iron metabolism in CHC patients.<\/jats:p>","DOI":"10.3390\/v17101302","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T12:33:03Z","timestamp":1758889983000},"page":"1302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Iron Metabolism Genes Shape the Course of Liver Fibrosis in Chronic Hepatitis C: From Disease Progression to Reversal After Direct-Acting Antivirals Treatment"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3527-3274","authenticated-orcid":false,"given":"Joana","family":"Ferreira","sequence":"first","affiliation":[{"name":"Institute for Scientific Research Bento Rocha Cabral, 1250-047 Lisbon, Portugal"},{"name":"Genetics Laboratory, Environmental Health Institute (ISAMB), Associated Laboratory TERRA, Lisbon Medical School, University of Lisbon, 1649-028 Lisbon; Higher Institute of Agronomy, 1349-017 Lisbon, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5773-5687","authenticated-orcid":false,"given":"Manuel","family":"Bicho","sequence":"additional","affiliation":[{"name":"Institute for Scientific Research Bento Rocha Cabral, 1250-047 Lisbon, Portugal"},{"name":"Genetics Laboratory, Environmental Health Institute (ISAMB), Associated Laboratory TERRA, Lisbon Medical School, University of Lisbon, 1649-028 Lisbon; Higher Institute of Agronomy, 1349-017 Lisbon, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6269-4867","authenticated-orcid":false,"given":"Paula","family":"Faustino","sequence":"additional","affiliation":[{"name":"Genetics Laboratory, Environmental Health Institute (ISAMB), Associated Laboratory TERRA, Lisbon Medical School, University of Lisbon, 1649-028 Lisbon; Higher Institute of Agronomy, 1349-017 Lisbon, Portugal"},{"name":"Human Genetics Department, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima","family":"Serejo","sequence":"additional","affiliation":[{"name":"Genetics Laboratory, Environmental Health Institute (ISAMB), Associated Laboratory TERRA, Lisbon Medical School, University of Lisbon, 1649-028 Lisbon; Higher Institute of Agronomy, 1349-017 Lisbon, Portugal"},{"name":"Gastroenterology and Hepatology Department, Hospital de Santa Maria, 1649-028 Lisbon, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gupta, S., Read, S.A., Shackel, N.A., Hebbard, L., George, J., and Ahlenstiel, G. 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