{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T08:40:15Z","timestamp":1759135215484,"version":"3.44.0"},"reference-count":61,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"North Carolina State University College of Veterinary Medicine"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Viruses"],"abstract":"<jats:p>Selenium (Se) is a trace mineral with antioxidant and anti-inflammatory properties. Se deficiency increases oxidative stress and immunosuppression. In swine, dietary Se supplementation enhances immunity and growth, and previous studies suggest it protects immune cells during viral infection. Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe respiratory and reproductive failure in swine, resulting in annual losses of 1.2 billion USD. Vaccine efficacy is hampered by the virus\u2019s high mutation rate, requiring alternative approaches. This study examines the effects of organic (DL-Selenomethionine, L-Selenomethionine, yeast-selenium) and inorganic (sodium selenite) Se on PRRSV infection in vitro. Porcine alveolar macrophages, the primary target of PRRSV in the lung, were isolated from healthy animals and infected with PRRSV-2 with or without Se. Mitochondrial function, gene expression, oxidative stress, and viral load were assessed post-infection. DL-selenomethionine showed increased glycolytic and mitochondrial ATP production relative to other compounds, suggesting improved mitochondrial function. No antiviral activity against PRRSV was observed. Transcriptome analysis revealed infection-driven modulation, with upregulation of IL6, IL8, IL1B1, MX1, and TXNRD1, but Se had no significant effect. While Se did not exhibit antiviral activity in vitro, its enhancement of mitochondrial function offers additional insight supporting its potential immunomodulatory benefits observed in previous in vivo studies.<\/jats:p>","DOI":"10.3390\/v17101303","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T14:50:38Z","timestamp":1758898238000},"page":"1303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Effects of Selenium Compounds on Mitochondrial Function in PRRSV-Infected Porcine Alveolar Macrophages"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7626-159X","authenticated-orcid":false,"given":"Abigail","family":"Williams","sequence":"first","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Bourne","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1533-7047","authenticated-orcid":false,"given":"John","family":"Byrne","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaitawat","family":"Sirisereewan","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittany M.","family":"Pecoraro","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4235-8508","authenticated-orcid":false,"given":"Elisa","family":"Crisci","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"ref_1","unstructured":"Roepke, D. (2024). Growing Losses from PRRS Cost Pork Producers $1.2 Billion Per Year, New Study Shows, Iowa State Research."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106627","DOI":"10.1016\/j.prevetmed.2025.106627","article-title":"Economic Impact of Productivity Losses Attributable to Porcine Reproductive And Respiratory Syndrome Virus in United States Pork Production, 2016 to 2020","volume":"244","author":"Osemeke","year":"2025","journal-title":"Prev. Vet. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e0070924","DOI":"10.1128\/msphere.00709-24","article-title":"PRRSV-2 variant classification: A dynamic nomenclature for enhanced monitoring and surveillance","volume":"10","author":"VanderWaal","year":"2025","journal-title":"mSphere"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zeller, M.A., Chang, J., Trevisan, G., Main, R.G., Gauger, P.C., and Zhang, J. (2024). Rapid PRRSV-2 ORF5-based lineage classification using Nextclade. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1419340"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"001632","DOI":"10.1099\/jgv.0.001632","article-title":"ICTV Virus Taxonomy Profile: Arteriviridae 2021","volume":"102","author":"Brinton","year":"2021","journal-title":"J. Gen. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s12026-014-8549-5","article-title":"Porcine reproductive and respiratory syndrome (PRRS): An immune dysregulatory pandemic","volume":"59","author":"Butler","year":"2014","journal-title":"Immunol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Cai, H., Zhang, H., Cheng, H., Liu, M., Wen, S., and Ren, J. (2023). Progress in PRRSV Infection and Adaptive Immune Response Mechanisms. Viruses, 15.","DOI":"10.3390\/v15071442"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Fiers, J., Cay, A.B., Maes, D., and Tignon, M. (2024). A Comprehensive Review on Porcine Reproductive and Respiratory Syndrome Virus with Emphasis on Immunity. Vaccines, 12.","DOI":"10.3390\/vaccines12080942"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Panahipoor Javaherdehi, A., Ghanbari, S., Mahdavi, P., Zafarani, A., and Razizadeh, M.H. (2024). The role of alveolar macrophages in viral respiratory infections and their therapeutic implications. Biochem. Biophys. Rep., 40.","DOI":"10.1016\/j.bbrep.2024.101826"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Melo, E.M., Oliveira, V.L.S., Boff, D., and Galv\u00e3o, I. (2021). Pulmonary macrophages and their different roles in health and disease. Int. J. Biochem. Cell Biol., 141.","DOI":"10.1016\/j.biocel.2021.106095"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Allard, B., Panariti, A., and Martin, J.G. (2018). Alveolar Macrophages in the Resolution of Inflammation, Tissue Repair, and Tolerance to Infection. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.01777"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Crisci, E., Fraile, L., and Montoya, M. (2019). Cellular Innate Immunity against PRRSV and Swine Influenza Viruses. Vet. Sci., 6.","DOI":"10.3390\/vetsci6010026"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"56002","DOI":"10.18632\/oncotarget.10817","article-title":"Porcine reproductive and respiratory syndrome virus triggers mitochondrial fission and mitophagy to attenuate apoptosis","volume":"7","author":"Li","year":"2016","journal-title":"Oncotarget"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s12011-017-1160-6","article-title":"Protective Role of Selenium in Immune-Relevant Cytokine and Immunoglobulin Production by Piglet Splenic Lymphocytes Exposed to Deoxynivalenol","volume":"184","author":"Wang","year":"2018","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.virol.2017.12.018","article-title":"Intercellular transfer of mitochondria rescues virus-induced cell death but facilitates cell-to-cell spreading of porcine reproductive and respiratory syndrome virus","volume":"517","author":"Guo","year":"2018","journal-title":"Virology"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Chae, C. (2021). Commercial PRRS Modified-Live Virus Vaccines. Vaccines, 9.","DOI":"10.3390\/vaccines9020185"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pecoraro, B.M., Leal, D.F., Frias-De-Diego, A., Browning, M., Odle, J., and Crisci, E. (2022). The health benefits of selenium in food animals: A review. J. Anim. Sci. Biotechnol., 13.","DOI":"10.1186\/s40104-022-00706-2"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Roh, Y.J., Han, S.-J., Park, I., Lee, H.M., Ok, Y.S., Lee, B.C., and Lee, S.-R. (2020). Role of Selenoproteins in Redox Regulation of Signaling and the Antioxidant System: A Review. Antioxidants, 9.","DOI":"10.3390\/antiox9050383"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6534","DOI":"10.1039\/D3NJ05180C","article-title":"Selenium compounds as promising antiviral agents","volume":"48","author":"Jain","year":"2024","journal-title":"New J. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Pila-Castellanos, I., Molino, D., McKellar, J., Lines, L., Da Graca, J., Tauziet, M., Chanteloup, L., Mikaelian, I., Meyniel-Schicklin, L., and Codogno, P. (2021). Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog., 17.","DOI":"10.1371\/journal.ppat.1009485"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Duan, C., Ma, R., Zeng, X., Chen, B., Hou, D., Liu, R., Li, X., Liu, L., Li, T., and Huang, H. (2022). SARS-CoV-2 Achieves Immune Escape by Destroying Mitochondrial Quality: Comprehensive Analysis of the Cellular Landscapes of Lung and Blood Specimens From Patients With COVID-19. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.946731"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3734","DOI":"10.1021\/acsanm.3c05307","article-title":"Zinc Oxide\u2013Selenium Nanoparticles for Inhibiting the Proliferation of Porcine Reproductive and Respiratory Syndrome Virus","volume":"7","author":"Xu","year":"2024","journal-title":"ACS Appl. Nano Mater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.freeradbiomed.2012.04.035","article-title":"Selenium blocks porcine circovirus type 2 replication promotion induced by oxidative stress by improving GPx1 expression","volume":"53","author":"Chen","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"txad049","DOI":"10.1093\/tas\/txad049","article-title":"Evaluation of selenium source on nursery pig growth performance, serum and tissue selenium concentrations, and serum antioxidant status","volume":"7","author":"Rao","year":"2023","journal-title":"Transl. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3945\/an.114.007575","article-title":"Dietary selenium in adjuvant therapy of viral and bacterial infections","volume":"6","author":"Steinbrenner","year":"2015","journal-title":"Adv. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.anifeedsci.2018.01.020","article-title":"The influence of selenium and selenoproteins on immune responses of poultry and pigs","volume":"238","author":"Dalgaard","year":"2018","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.vetimm.2018.12.002","article-title":"Dietary selenium supplementation enhances antiviral immunity in chickens challenged with low pathogenic avian influenza virus subtype H9N2","volume":"207","author":"Shojadoost","year":"2019","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Frias-De-Diego, A., Gilbertie, J.M., Scholle, F., Dejarnette, S., and Crisci, E. (2022). Effect of BIO-PLYTM, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages. Viruses, 14.","DOI":"10.3390\/v14122666"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Bordet, E., Blanc, F., Tiret, M., Crisci, E., Bouguyon, E., Renson, P., Maisonnasse, P., Bourge, M., Leplat, J.-J., and Giuffra, E. (2018). Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.02299"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Paploski, I.A.D., Corzo, C., Rovira, A., Murtaugh, M.P., Sanhueza, J.M., Vilalta, C., Schroeder, D.C., and VanderWaal, K. (2019). Temporal Dynamics of Co-circulating Lineages of Porcine Reproductive and Respiratory Syndrome Virus. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02486"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"VanderWaal, K., Pamornchainavakul, N., Kikuti, M., Linhares, D.C.L., Trevisan, G., Zhang, J., Anderson, T.K., Zeller, M., Rossow, S., and Holtkamp, D.J. (2024). Phylogenetic-based methods for fine-scale classification of PRRSV-2 ORF5 sequences: A comparison of their robustness and reproducibility. Front. Virol., 4.","DOI":"10.3389\/fviro.2024.1433931"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kick, A.R., Amaral, A.F., Cortes, L.M., Fogle, J.E., Crisci, E., Almond, G.W., and K\u00e4ser, T. (2019). The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Viruses, 11.","DOI":"10.3390\/v11090796"},{"key":"ref_33","first-page":"e63063","article-title":"Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays","volume":"12","author":"Crisci","year":"2022","journal-title":"J. Vis. Exp. (JoVE)"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s12250-020-00230-5","article-title":"On the Calculation of TCID(50) for Quantitation of Virus Infectivity","volume":"36","author":"Lei","year":"2021","journal-title":"Virol. Sin."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5769752","DOI":"10.1155\/2019\/5769752","article-title":"Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite","volume":"2019","author":"Ren","year":"2019","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Elesela, S., and Lukacs, N.W. (2021). Role of Mitochondria in Viral Infections. Life, 11.","DOI":"10.3390\/life11030232"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kim, S.-J., Khan, M., Quan, J., Till, A., Subramani, S., and Siddiqui, A. (2013). Hepatitis B Virus Disrupts Mitochondrial Dynamics: Induces Fission and Mitophagy to Attenuate Apoptosis. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003722"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.virol.2007.04.001","article-title":"Porcine reproductive and respiratory syndrome virus induces apoptosis through a mitochondria-mediated pathway","volume":"365","author":"Lee","year":"2007","journal-title":"Virology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5969","DOI":"10.3390\/nu7075263","article-title":"Serum Concentrations of Trace Elements in Patients with Tuberculosis and Its Association with Treatment Outcome","volume":"7","author":"Choi","year":"2015","journal-title":"Nutrients"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Mehta, S.L., Kumari, S., Mendelev, N., and Li, P.A. (2012). Selenium preserves mitochondrial function, stimulates mitochondrial biogenesis, and reduces infarct volume after focal cerebral ischemia. BMC Neurosci., 13.","DOI":"10.1186\/1471-2202-13-79"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/s41580-020-0230-3","article-title":"Reactive oxygen species (ROS) as pleiotropic physiological signalling agents","volume":"21","author":"Sies","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Canton, M., S\u00e1nchez-Rodr\u00edguez, R., Spera, I., Venegas, F.C., Favia, M., Viola, A., and Castegna, A. (2021). Reactive Oxygen Species in Macrophages: Sources and Targets. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.734229"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s13567-019-0679-2","article-title":"Xanthohumol inhibits PRRSV proliferation and alleviates oxidative stress induced by PRRSV via the Nrf2\u2013HMOX1 axis","volume":"50","author":"Liu","year":"2019","journal-title":"Vet. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1007\/s10753-017-0566-9","article-title":"Regulation of iNOS-Derived ROS Generation by HSP90 and Cav-1 in Porcine Reproductive and Respiratory Syndrome Virus-Infected Swine Lung Injury","volume":"40","author":"Yan","year":"2017","journal-title":"Inflammation"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s12011-019-01908-y","article-title":"Selenium Deficiency Induces Inflammation via the iNOS\/NF-\u03baB Pathway in the Brain of Pigs","volume":"196","author":"Zhang","year":"2020","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Martinez, S.S., Huang, Y., Acuna, L., Laverde, E., Trujillo, D., Barbieri, M.A., Tamargo, J., Campa, A., and Baum, M.K. (2021). Role of Selenium in Viral Infections with a Major Focus on SARS-CoV-2. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010280"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1089\/aid.1994.10.1451","article-title":"Stimulation of Glutathione Peroxidase Activity Decreases HIV Type 1 Activation after Oxidative Stress","volume":"10","author":"SAPPEY","year":"1994","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.jtemb.2006.11.001","article-title":"Activation of superoxide dismutase in selenium-deficient mice infected with influenza virus","volume":"21","author":"Walton","year":"2007","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.2147\/IJN.S370585","article-title":"Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS\/JNK-Mediated Apoptosis in vitro","volume":"17","author":"Shao","year":"2022","journal-title":"Int. J. Nanomed."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e0105221","DOI":"10.1128\/JVI.01052-21","article-title":"Porcine Reproductive and Respiratory Syndrome Virus Infection Upregulates Negative Immune Regulators and T-Cell Exhaustion Markers","volume":"95","author":"Chaudhari","year":"2021","journal-title":"J. Virol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev-animal-022114-111025","article-title":"Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System","volume":"4","author":"Lunney","year":"2016","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Badaoui, B., Tuggle, C.K., Hu, Z., Reecy, J.M., Ait-Ali, T., Anselmo, A., and Botti, S. (2013). Pig immune response to general stimulus and to porcine reproductive and respiratory syndrome virus infection: A meta-analysis approach. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-220"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1099\/vir.0.2008\/003244-0","article-title":"Genome-wide transcriptional response of primary alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus","volume":"89","author":"Genini","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Liu, G., Yang, G., Guan, G., Zhang, Y., Ren, W., Yin, J., Aguilar, Y.M., Luo, W., Fang, J., and Yu, X. (2015). Effect of dietary selenium yeast supplementation on porcine circovirus type 2 (PCV2) infections in mice. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0115833"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Ren, Z., Jia, G., He, H., Ding, T., Yu, Y., Zuo, Z., Hu, Y., Zhong, Z., Yu, S., and Deng, H. (2022). Antiviral Effect of Selenomethionine on Porcine Deltacoronavirus in Pig Kidney Epithelial Cells. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.846747"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1002\/jmv.24366","article-title":"Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines","volume":"88","author":"Cheng","year":"2016","journal-title":"J. Med. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1152\/physrev.00039.2013","article-title":"Selenoproteins: Molecular Pathways and Physiological Roles","volume":"94","author":"Labunskyy","year":"2014","journal-title":"Physiol. Rev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s12199-007-0019-4","article-title":"Selenium: Its role as antioxidant in human health","volume":"13","author":"Tinggi","year":"2008","journal-title":"Environ. Health Prev. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"20469","DOI":"10.18632\/oncotarget.7814","article-title":"Overexpression of pig selenoprotein S blocks OTA-induced promotion of PCV2 replication by inhibiting oxidative stress and p38 phosphorylation in PK15 cells","volume":"7","author":"Gan","year":"2016","journal-title":"Oncotarget"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1183\/16000617.0031-2015","article-title":"The heterogeneity of lung macrophages in the susceptibility to disease","volume":"24","author":"Misharin","year":"2015","journal-title":"Eur. Respir. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1097\/00042560-199708150-00007","article-title":"High Risk of HIV-Related Mortality Is Associated With Selenium Deficiency","volume":"15","author":"Baum","year":"1997","journal-title":"JAIDS J. Acquir. Immune Defic. Syndr."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/10\/1303\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T08:03:11Z","timestamp":1759132991000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/10\/1303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,26]]},"references-count":61,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["v17101303"],"URL":"https:\/\/doi.org\/10.3390\/v17101303","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2025,9,26]]}}}