{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T05:23:57Z","timestamp":1761801837946,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["309367\/2021-2"],"award-info":[{"award-number":["309367\/2021-2"]}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"crossref","award":["E-26\/211.460\/2021","E-26\/211.344\/2021","E-26\/200.407\/2023"],"award-info":[{"award-number":["E-26\/211.460\/2021","E-26\/211.344\/2021","E-26\/200.407\/2023"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Dengue and COVID-19 are viral diseases characterized by coagulopathies, with Dengue associated with fibrinolysis and COVID-19 with prothrombotic events. Furthermore, cross-reactivity between anti-SARS-CoV-2 and anti-DENV antibodies may confer protective immunity or exacerbate disease severity. Our investigation explored the impact of prior COVID-19 exposure on the immunopathogenesis of Dengue, focusing on hemostatic parameters. We quantified nitrites, procoagulant, anticoagulant, and fibrinolytic mediators in the plasma of Dengue patients before and after the COVID-19 pandemic. We also evaluated the influence of plasma from dengue patients on platelet activation in vitro using platelets from healthy donors exposed to DENV-2. Hemorrhagic manifestations were more frequent in pre-COVID-19 Dengue, while nitrite levels were elevated in post-COVID-19 Dengue patients, particularly among those without hemorrhagic signs. Among procoagulant factors, tissue factor (TF) levels were increased in post-COVID-19 Dengue, whereas Factor XIII was higher in pre-COVID-19 Dengue. In contrast, antithrombin (an anticoagulant) and plasminogen (a profibrinolytic factor) were more elevated in pre-COVID-19 Dengue than in post-COVID-19 cases. In vitro, DENV-2-infected platelets exposed to plasma of Dengue patients before and after COVID-19 showed decreased nitrite production in relation to DENV-2 alone. These findings suggest that prior COVID-19 exposure may influence hemostatic responses in Dengue, potentially modulating disease pathophysiology and opening new avenues for research and therapeutic strategies.<\/jats:p>","DOI":"10.3390\/v17111431","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:59:36Z","timestamp":1761659976000},"page":"1431","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Profile of Hemostasis in Dengue Fever Before and After COVID-19"],"prefix":"10.3390","volume":"17","author":[{"given":"Alanna Calheiros","family":"Santos","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio das Intera\u00e7\u00f5es V\u00edrus-Hospedeiros, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julya Farias","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio das Intera\u00e7\u00f5es V\u00edrus-Hospedeiros, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Paula","family":"de Souza e Silva Sales","sequence":"additional","affiliation":[{"name":"Centro de Doen\u00e7as Imuno-Infecciosas, Hospital dos Plantadores de Cana, Campos dos Goytacazes, Rio de Janeiro 28025-496, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3603-6921","authenticated-orcid":false,"given":"Mariana","family":"Gandini","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Celular, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1309-5366","authenticated-orcid":false,"given":"Fl\u00e1via Barreto","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio das Intera\u00e7\u00f5es V\u00edrus-Hospedeiros, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Vieira","family":"Damasco","sequence":"additional","affiliation":[{"name":"Rede Casa Hospital Rio Laranjeiras e Rio Botafogo, Rio de Janeiro 22240-000, Brazil"},{"name":"Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, Escola de Medicina e Cirurgia, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 20270-004, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Jos\u00e9","family":"de Souza","sequence":"additional","affiliation":[{"name":"Centro de Doen\u00e7as Imuno-Infecciosas, Hospital dos Plantadores de Cana, Campos dos Goytacazes, Rio de Janeiro 28025-496, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elzinandes Leal","family":"de Azeredo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio das Intera\u00e7\u00f5es V\u00edrus-Hospedeiros, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luzia Maria","family":"de-Oliveira-Pinto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio das Intera\u00e7\u00f5es V\u00edrus-Hospedeiros, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"ref_1","first-page":"270","article-title":"COVID-19 and dengue: A deadly duo","volume":"50","author":"Ridwan","year":"2020","journal-title":"Trop. 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