{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:48:47Z","timestamp":1790113727380,"version":"4.0.1"},"reference-count":68,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Acinetobacter baumannii is a critical public health threat, particularly with the rise in multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains that limit treatment options. Phage therapy, which uses bacteriophages to target bacteria, offers a promising alternative. We isolated an XDR strain (Ab125) from a burn wound infection and screened 34 phages, identifying vB_AbaM_3098 as the only effective candidate. However, resistance rapidly emerged, producing a derivative strain (Ab139). Interestingly, Ab139, though resistant to vB_AbaM_3098, became susceptible to six previously inactive phages. While various potential determinants were identified through comparative genomics and proteomics, the mechanism causing phage resistance to vB_AbaM_3098 and simultaneous susceptibility to other phages remains to be elucidated. Among the six new candidates, vB_AbaM_3014 was the most promising. While each phage alone allowed bacterial regrowth, combining vB_AbaM_3098 and vB_AbaM_3014 completely suppressed Ab125 growth. In a Galleria mellonella infection model, this cocktail achieved 90% survival after five days compared to 0% in untreated controls. Notably, the cocktail combined one phage with modest activity and another inactive phage against the parental strain; together, they produced strong bactericidal effects. These findings highlight both the complexity of phage cocktail design and their promise as adjunct therapies against drug-resistant A. baumannii.<\/jats:p>","DOI":"10.3390\/v17111441","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T17:38:22Z","timestamp":1761759502000},"page":"1441","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rational Design of a Potent Two-Phage Cocktail Against a Contemporary Acinetobacter baumannii Strain Recovered from a Burned Patient at the Lausanne University Hospital"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5274-8979","authenticated-orcid":false,"given":"Hugues","family":"de Villiers de la Noue","sequence":"first","affiliation":[{"name":"Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Clinical Pharmaceutical Sciences (CRISP), Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwena\u00eblle","family":"Golliard","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Clinical Pharmaceutical Sciences (CRISP), Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Vuattoux","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Clinical Pharmaceutical Sciences (CRISP), Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2341-4591","authenticated-orcid":false,"given":"Gr\u00e9gory","family":"Resch","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Clinical Pharmaceutical Sciences (CRISP), Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6987","DOI":"10.1007\/s11033-021-06690-6","article-title":"Multidrug-resistant Acinetobacter baumannii as an emerging concern in hospitals","volume":"48","author":"Ibrahim","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/2049-632X.12125","article-title":"Acinetobacter baumannii: Evolution of a global pathogen","volume":"71","author":"Antunes","year":"2014","journal-title":"Pathog. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: The WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"ref_4","unstructured":"WHO (2022). Global Antimicrobial Resistance and Use Surveillance System (GLASS) Report 2022, WHO."},{"key":"ref_5","first-page":"1","article-title":"Antimicrobial-resistant pathogens associated with adult healthcare-associated infections: Summary of data reported to the National Healthcare Safety Network, 2015\u20132017","volume":"41","author":"Abner","year":"2020","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1139\/gen-2013-0152","article-title":"Viruses: Unlocking the greatest biodiversity on Earth","volume":"56","author":"Suttle","year":"2013","journal-title":"Genome"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1126\/science.1174173","article-title":"Local Adaptation of Bacteriophages to Their Bacterial Hosts in Soil","volume":"325","author":"Vos","year":"2009","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/s41579-019-0311-5","article-title":"Phage diversity, genomics and phylogeny","volume":"18","author":"Dion","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1016\/j.mib.2013.08.008","article-title":"Phage lysis: Do we have the hole story yet?","volume":"16","author":"Young","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Principi, N., Silvestri, E., and Esposito, S. (2019). Advantages and Limitations of Bacteriophages for the Treatment of Bacterial Infections. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.00513"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1128\/AAC.45.3.649-659.2001","article-title":"Bacteriophage therapy","volume":"45","author":"Sulakvelidze","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1038\/s41564-024-01705-x","article-title":"Personalized bacteriophage therapy outcomes for 100 consecutive cases: A multicentre, multinational, retrospective observational study","volume":"9","author":"Pirnay","year":"2024","journal-title":"Nat. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Pirnay, J.P., Ferry, T., and Resch, G. (2022). Recent progress toward the implementation of phage therapy in Western medicine. FEMS Microbiol. Rev., 46.","DOI":"10.1093\/femsre\/fuab040"},{"key":"ref_14","unstructured":"(2025, March 26). Available online: https:\/\/www.clinicaltrials.gov\/search?intr=Phage%20Therapy."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/AAC.00954-17","article-title":"Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection","volume":"61","author":"Schooley","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_16","unstructured":"CLSI (2015). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, CLSI. Approved Standards."},{"key":"ref_17","unstructured":"Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data, Babraham Bioinformatics."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"W601","DOI":"10.1093\/nar\/gkad406","article-title":"WebQUAST: Online evaluation of genome assemblies","volume":"51","author":"Mikheenko","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/ng.806","article-title":"A framework for variation discovery and genotyping using next-generation DNA sequencing data","volume":"43","author":"DePristo","year":"2011","journal-title":"Nat. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Vasimuddin, M., Misra, S., Li, H., and Aluru, S. (2019, January 20\u201324). Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems. Proceedings of the 2019 IEEE International Parallel and Distributed Processing Symposium (IPDPS), Rio de Janeiro, Brazil.","DOI":"10.1109\/IPDPS.2019.00041"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Bouras, G., Nepal, R., Houtak, G., Psaltis, A.J., Wormald, P.J., and Vreugde, S. (2023). Pharokka: A fast scalable bacteriophage annotation tool. Bioinformatics, 39.","DOI":"10.1093\/bioinformatics\/btac776"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"von Meijenfeldt, F.A.B., Arkhipova, K., Cambuy, D.D., Coutinho, F.H., and Dutilh, B.E. (2019). Robust taxonomic classification of uncharted microbial sequences and bins with CAT and BAT. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1817-x"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e11396","DOI":"10.7717\/peerj.11396","article-title":"BACPHLIP: Predicting bacteriophage lifestyle from conserved protein domains","volume":"9","author":"Hockenberry","year":"2021","journal-title":"PeerJ"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1089\/phage.2019.0001","article-title":"The Virulence Index: A Metric for Quantitative Analysis of Phage Virulence","volume":"1","author":"Storms","year":"2020","journal-title":"PHAGE"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Nikolic, I., Vukovic, D., Gavric, D., Cvetanovic, J., Aleksic Sabo, V., Gostimirovic, S., Narancic, J., and Knezevic, P. (2022). An Optimized Checkerboard Method for Phage-Antibiotic Synergy Detection. Viruses, 14.","DOI":"10.3390\/v14071542"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) (2000). Terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents. Clin. Microbiol. Infect., 6, 503\u2013508.","DOI":"10.1046\/j.1469-0691.2000.00149.x"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s41596-018-0082-x","article-title":"Single-pot, solid-phase-enhanced sample preparation for proteomics experiments","volume":"14","author":"Hughes","year":"2019","journal-title":"Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1021\/pr101065j","article-title":"Andromeda: A peptide search engine integrated into the MaxQuant environment","volume":"10","author":"Cox","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s40265-024-02104-6","article-title":"Treatment Approaches for Carbapenem-Resistant Acinetobacter baumannii Infections","volume":"85","author":"Iovleva","year":"2025","journal-title":"Drugs"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"106674","DOI":"10.1016\/j.micpath.2024.106674","article-title":"An insight into MDR Acinetobacter baumannii infection and its pathogenesis: Potential therapeutic targets and challenges","volume":"192","author":"Singh","year":"2024","journal-title":"Microb. Pathog."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Manley, R., Fitch, C., Francis, V., Temperton, I., Turner, D., Fletcher, J., Phil, M., Michell, S., and Temperton, B. (2024). Resistance to bacteriophage incurs a cost to virulence in drug-resistant Acinetobacter baumannii. J. Med. Microbiol., 73.","DOI":"10.1099\/jmm.0.001829"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Bai, J., Raustad, N., Denoncourt, J., van Opijnen, T., and Geisinger, E. (2023). Genome-wide phage susceptibility analysis in Acinetobacter baumannii reveals capsule modulation strategies that determine phage infectivity. PLoS Pathog., 19.","DOI":"10.1371\/journal.ppat.1010928"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6249","DOI":"10.1093\/nar\/gkn633","article-title":"Overexpression of the LexA-regulated tisAB RNA in E. coli inhibits SOS functions; implications for regulation of the SOS response","volume":"36","author":"Bjoras","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/s00018-008-8378-6","article-title":"The bacterial LexA transcriptional repressor","volume":"66","author":"Butala","year":"2009","journal-title":"Cell Mol. Life Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"7315","DOI":"10.1093\/nar\/gkv634","article-title":"Bacteriophage GIL01 gp7 interacts with host LexA repressor to enhance DNA binding and inhibit RecA-mediated auto-cleavage","volume":"43","author":"Fornelos","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1021\/acsinfecdis.1c00021","article-title":"The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors","volume":"7","author":"Tram","year":"2021","journal-title":"ACS Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1080\/21505594.2018.1558693","article-title":"The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii","volume":"10","author":"Weidensdorfer","year":"2019","journal-title":"Virulence"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Chan, B.K., Sistrom, M., Wertz, J.E., Kortright, K.E., Narayan, D., and Turner, P.E. (2016). Phage selection restores antibiotic sensitivity in MDR Pseudomonas aeruginosa. Sci. Rep., 6.","DOI":"10.1038\/srep26717"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1186\/s12985-024-02556-y","article-title":"Characterization of phage HZY2308 against Acinetobacter baumannii and identification of phage-resistant bacteria","volume":"21","author":"Wang","year":"2024","journal-title":"Virol. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/s41564-020-00830-7","article-title":"Bacteriophage-resistant Acinetobacter baumannii are resensitized to antimicrobials","volume":"6","author":"Forsyth","year":"2021","journal-title":"Nat. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1080\/22221751.2021.2002671","article-title":"Colistin-phage combinations decrease antibiotic resistance in Acinetobacter baumannii via changes in envelope architecture","volume":"10","author":"Wang","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1007\/s11095-014-1617-7","article-title":"Quality and safety requirements for sustainable phage therapy products","volume":"32","author":"Pirnay","year":"2015","journal-title":"Pharm. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"100077","DOI":"10.1016\/j.infpip.2020.100077","article-title":"Control and management of multidrug resistant Acinetobacter baumannii: A review of the evidence and proposal of novel approaches","volume":"2","author":"Weinberg","year":"2020","journal-title":"Infect. Prev. Pract."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Chaudhry, W.N., Concepcion-Acevedo, J., Park, T., Andleeb, S., Bull, J.J., and Levin, B.R. (2017). Synergy and Order Effects of Antibiotics and Phages in Killing Pseudomonas aeruginosa Biofilms. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0168615"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Save, J., Que, Y.A., Entenza, J., and Resch, G. (2022). Subtherapeutic Doses of Vancomycin Synergize with Bacteriophages for Treatment of Experimental Methicillin-Resistant Staphylococcus aureus Infective Endocarditis. Viruses, 14.","DOI":"10.1101\/2022.06.02.494631"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e023080","DOI":"10.1161\/JAHA.121.023080","article-title":"Bacteriophages Combined With Subtherapeutic Doses of Flucloxacillin Act Synergistically Against Staphylococcus aureus Experimental Infective Endocarditis","volume":"11","author":"Save","year":"2022","journal-title":"J. Am. Heart Assoc."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Madison, C.L., Steinert, A.S.J., Luedeke, C.E., Hajjafar, N., Srivastava, P., Berti, A.D., Bayer, A.S., and Kebriaei, R. (2024). It takes two to tango: Preserving daptomycin efficacy against daptomycin-resistant MRSA using daptomycin-phage co-therapy. Microbiol. Spectr., 12.","DOI":"10.1128\/spectrum.00679-24"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kunz Coyne, A.J., Eshaya, M., Bleick, C., Vader, S., Biswas, B., Wilson, M., Deschenes, M.V., Alexander, J., Lehman, S.M., and Rybak, M.J. (2024). Exploring synergistic and antagonistic interactions in phage-antibiotic combinations against ESKAPE pathogens. Microbiol. Spectr., 12.","DOI":"10.1128\/spectrum.00427-24"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.3390\/v6103778","article-title":"Effect of bacteriophage infection in combination with tobramycin on the emergence of resistance in Escherichia coli and Pseudomonas aeruginosa biofilms","volume":"6","author":"Coulter","year":"2014","journal-title":"Viruses"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e2216084120","DOI":"10.1073\/pnas.2216084120","article-title":"Antibiotics that affect translation can antagonize phage infectivity by interfering with the deployment of counter-defenses","volume":"120","author":"Pons","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_57","unstructured":"CHUV (2025, January 01). DM_DAM_Guide_Antibiotherapie. Available online: https:\/\/www.chuv.ch\/fileadmin\/sites\/min\/552801_22_DM_DAM_guide_antibiotherapie_version_mai_2022.pdf."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.jgar.2019.05.005","article-title":"Efficacy of newly isolated and highly potent bacteriophages in a mouse model of extensively drug-resistant Acinetobacter baumannii bacteraemia","volume":"19","author":"Leshkasheli","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1007\/s11262-024-02103-5","article-title":"Isolation, characterization, and potential application of Acinetobacter baumannii phages against extensively drug-resistant strains","volume":"60","author":"Rastegar","year":"2024","journal-title":"Virus Genes"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e0046724","DOI":"10.1128\/jvi.00467-24","article-title":"In vivo efficacy of phage cocktails against carbapenem resistance Acinetobacter baumannii in the rat pneumonia model","volume":"98","author":"Li","year":"2024","journal-title":"J. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Jeon, J., Park, J.H., and Yong, D. (2019). Efficacy of bacteriophage treatment against carbapenem-resistant Acinetobacter baumannii in Galleria mellonella larvae and a mouse model of acute pneumonia. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1443-5"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Qu, J., Zou, J., Zhang, J., Qu, J., and Lu, H. (2024). Phage therapy for extensively drug resistant Acinetobacter baumannii infection: Case report and in vivo evaluation of the distribution of phage and the impact on gut microbiome. Front. Med., 11.","DOI":"10.3389\/fmed.2024.1432703"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Tan, X., Chen, H., Zhang, M., Zhao, Y., Jiang, Y., Liu, X., Huang, W., and Ma, Y. (2021). Clinical Experience of Personalized Phage Therapy Against Carbapenem-Resistant Acinetobacter baumannii Lung Infection in a Patient With Chronic Obstructive Pulmonary Disease. Front. Cell Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.631585"},{"key":"ref_64","first-page":"100565","article-title":"Randomized double-blind study on safety and tolerability of TP-102 phage cocktail in patients with infected and non-infected diabetic foot ulcers","volume":"6","author":"Onallah","year":"2024","journal-title":"Med"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e0199621","DOI":"10.1128\/AAC.01996-21","article-title":"Case Commentary: Novel Therapy for Multidrug-Resistant Acinetobacter baumannii Infection","volume":"66","author":"Eales","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1080\/22221751.2021.1902754","article-title":"Pre-optimized phage therapy on secondary Acinetobacter baumannii infection in four critical COVID-19 patients","volume":"10","author":"Wu","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1093\/cid\/ciz222","article-title":"Successful Treatment of Antibiotic-resistant, Poly-microbial Bone Infection With Bacteriophages and Antibiotics Combination","volume":"69","author":"Gelman","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Gu, J., Liu, X., Li, Y., Han, W., Lei, L., Yang, Y., Zhao, H., Gao, Y., Song, J., and Lu, R. (2012). A method for generation phage cocktail with great therapeutic potential. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0031698"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/11\/1441\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T01:33:28Z","timestamp":1761874408000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/11\/1441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":68,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["v17111441"],"URL":"https:\/\/doi.org\/10.3390\/v17111441","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]}}}