{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T18:18:34Z","timestamp":1782843514064,"version":"3.54.5"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008370","name":"US National Pork Board","doi-asserted-by":"crossref","award":["NPB #20-252"],"award-info":[{"award-number":["NPB #20-252"]}],"id":[{"id":"10.13039\/100008370","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Canadian Food Inspection Agency (CFIA) ASF intramural funds"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>African swine fever (ASF) continues to spread across the globe, causing a severe impact on the swine industry. Passive surveillance based on testing dead pigs is one of the most effective methods for early detection of ASF incursions. We have previously shown that the superficial inguinal lymph node (SILN) is a convenient and effective sample type for ASF virus (ASFV) genome detection in pigs succumbed to highly or moderately virulent ASFV infections. In this study, we explored the distribution kinetics of ASFV into SILN and other lymphoid tissues in pigs exposed to moderately virulent ASFV strains (ASFV Estonia 2014 and ASFV Malta\u201978), oronasally. The ASFV genome was detected in SILNs as early as 2\u20133 days post-infection (dpi), peaking around 5\u20139 dpi. The detection of ASFV Estonia 2014 started early, and the pigs succumbed to infection faster compared to the ASFV Malta\u201978 infected pigs that remained longer. All pigs that succumbed to ASF had comparable levels of ASFV genomic material in the spleen and SILNs. The levels of ASFV genomic material gradually started to decrease in pigs that did not succumb to ASF, indicating possible virus clearance. In contrast, ASFV genome levels in blood and spleen samples remained relatively steady during the study period. Immunohistochemistry and in situ hybridization of spleen and SILN samples supported real-time PCR results. This study demonstrates the distribution kinetics of moderately virulent ASFV in peripheral lymph nodes and highlights the utility of SILNs for dead pig screening.<\/jats:p>","DOI":"10.3390\/v17111472","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:11:16Z","timestamp":1762254676000},"page":"1472","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Kinetics of Viral Genome Distribution in Swine Peripheral Lymphoid Organs Following Oronasal Infection with Attenuated African swine fever virus strains"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1949-7961","authenticated-orcid":false,"given":"Kalhari","family":"Goonewardene","sequence":"first","affiliation":[{"name":"National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada"},{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carissa","family":"Embury-Hyatt","sequence":"additional","affiliation":[{"name":"National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estella","family":"Moffat","sequence":"additional","affiliation":[{"name":"National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1783-2105","authenticated-orcid":false,"given":"Aruna","family":"Ambagala","sequence":"additional","affiliation":[{"name":"National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada"},{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"},{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"ref_1","unstructured":"WOAH WAHIS (2025, January 01). 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