{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T05:38:45Z","timestamp":1777354725781,"version":"3.51.4"},"reference-count":67,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014188","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2024-00336046"],"award-info":[{"award-number":["RS-2024-00336046"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["KIMST-20220128"],"award-info":[{"award-number":["KIMST-20220128"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002631","name":"Gachon University","doi-asserted-by":"crossref","award":["GCU-202401090001"],"award-info":[{"award-number":["GCU-202401090001"]}],"id":[{"id":"10.13039\/501100002631","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The emergence of multidrug-resistant Shiga toxin-producing Escherichia coli (STEC) poses a major challenge to public health and necessitates the development of alternative antimicrobial strategies. This study aimed to isolate and characterize five lytic bacteriophages belonging to the genus Mosigvirus and evaluate their potential as biocontrol against MDR STEC strains and their biofilms. The five bacteriophages, designated vB_EcoM-pJBB (\u03a6B), vB_EcoM-pJBC (\u03a6C), vB_EcoM-pJBJ (\u03a6J), vB_EcoM-pJBK (\u03a6K), and vB_EcoM-pJBL (\u03a6L), were isolated from sewage treatment plant samples using STEC ATCC 43895 as host. Biological characterization included host range determination against 19 MDR STEC strains, one-step growth analysis, environmental stability assays, bacteriolytic activity assessment, and antibiofilm efficacy testing. Whole-genome sequencing and phylogenetic analyses were performed to determine genomic features and taxonomic classification. The phages demonstrated varying infectious capacities, lysing between six and 12 strains, with \u03a6L exhibiting the broadest spectrum of activity. All phages showed MOI-independent antibiofilm activity, preventing biofilm formation by approximately 70% and disrupting pre-formed biofilms by up to 80.3%. Genomic analysis revealed the absence of lysogeny markers, virulence factors, and antimicrobial resistance genes, while identifying putative depolymerase genes associated with tail fiber proteins. Phylogenetic analysis confirmed the taxonomic position of these phages within the Mosigvirus genus in the Straboviridae family. Our findings indicate that the newly identified Mosigvirus phages are promising candidates for phage-based biocontrol applications.<\/jats:p>","DOI":"10.3390\/v17111501","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:57:13Z","timestamp":1763038633000},"page":"1501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of Five Lytic Bacteriophages as New Members of the Genus Mosigvirus, Infecting Multidrug-Resistant Shiga Toxin-Producing Escherichia coli and Their Antibiofilm Activity"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1482-5633","authenticated-orcid":false,"given":"Jong Beom","family":"Na","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seungki","family":"Lee","sequence":"additional","affiliation":[{"name":"Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8032-9427","authenticated-orcid":false,"given":"Eun Jeong","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soojin","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keeman","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye Bin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae Seon","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1351-7557","authenticated-orcid":false,"given":"Seon Young","family":"Park","sequence":"additional","affiliation":[{"name":"Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7921-2625","authenticated-orcid":false,"given":"Ji Hyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, College of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1093\/cid\/ciw099","article-title":"Shiga toxin\u2013producing Escherichia coli infection, antibiotics, and risk of developing hemolytic uremic syndrome: A meta-analysis","volume":"62","author":"Freedman","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Melton-Celsa, A.R. (2014). Shiga toxin (Stx) classification, structure, and function. Microbiol. Spectr., 2.","DOI":"10.1128\/microbiolspec.EHEC-0024-2013"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Pakbin, B., Br\u00fcck, W.M., and Rossen, J.W.A. (2021). Virulence factors of enteric pathogenic Escherichia coli: A review. Int. J. Mol. Sci., 22.","DOI":"10.20944\/preprints202108.0348.v1"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.ijfoodmicro.2012.08.008","article-title":"Review of Shiga-toxin-producing Escherichia coli (STEC) and their significance in dairy production","volume":"162","author":"Farrokh","year":"2013","journal-title":"Int. J. Food Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kim, J.S., Lee, M.S., and Kim, J.H. (2020). Recent updates on outbreaks of Shiga toxin-producing Escherichia coli and its potential reservoirs. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.00273"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/zph.12533","article-title":"Antibiotic-resistant Shiga toxin-producing Escherichia coli: An overview of prevalence and intervention strategies","volume":"66","author":"Mir","year":"2019","journal-title":"Zoonoses Public Health"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mandujano-Hern\u00e1ndez, A., Mart\u00ednez-V\u00e1zquez, A.V., Paz-Gonz\u00e1lez, A.D., Herrera-Mayorga, V., S\u00e1nchez-S\u00e1nchez, M., Lara-Ram\u00edrez, E.E., V\u00e1zquez, K., de Jes\u00fas de Luna-Santillana, E., Bocanegra-Garc\u00eda, V., and Rivera, G. (2024). The global rise of ESBL-producing Escherichia coli in the livestock sector: A five-year overview. Animals, 14.","DOI":"10.3390\/ani14172490"},{"key":"ref_8","unstructured":"World Health Organization (2024). WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance, to Guide Research, Development, and Strategies to Prevent and Control Antimicrobial Resistance, World Health Organization."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ullah, S., Khan, S.U.H., Khan, M.J., Khattak, B., Fozia, F., Ahmad, I., Wadaan, M.A., Khan, M.F., Baabbad, A., and Goyal, S.M. (2024). Multiple-drug resistant Shiga toxin-producing Escherichia coli in raw milk of dairy bovine. Trop. Med. Infect. Dis., 9.","DOI":"10.3390\/tropicalmed9030064"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jinf.2019.05.018","article-title":"Shiga toxin-induced haemolytic uraemic syndrome and the role of antibiotics: A global overview","volume":"79","author":"Kakoullis","year":"2019","journal-title":"J. Infect."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1099\/mic.0.000003","article-title":"Shiga toxins and stx phages: Highly diverse entities","volume":"161","author":"Lucchesi","year":"2015","journal-title":"Microbiology"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Rubio, L., Haarmann, N., Schwidder, M., Muniesa, M., and Schmidt, H. (2021). Bacteriophages of Shiga toxin-producing Escherichia coli and their contribution to pathogenicity. Pathogens, 10.","DOI":"10.3390\/pathogens10040404"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1055\/s-0043-1772707","article-title":"Prophage and plasmid-mediated beta-lactamases in multidrug-resistant extraintestinal Escherichia coli","volume":"14","author":"Raj","year":"2024","journal-title":"J. Health Allied Sci. NU"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/S0966-842X(00)01913-2","article-title":"Mechanisms of biofilm resistance to antimicrobial agents","volume":"9","author":"Mah","year":"2001","journal-title":"Trends Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ma, Z., Bumunang, E.W., Stanford, K., Bie, X., Niu, Y.D., and McAllister, T.A. (2019). Biofilm formation by Shiga toxin-producing Escherichia coli on stainless steel coupons as affected by temperature and incubation time. Microorganisms, 7.","DOI":"10.3390\/microorganisms7040095"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"162","DOI":"10.4292\/wjgpt.v8.i3.162","article-title":"Phage therapy: An alternative to antibiotics in the age of multi-drug resistance","volume":"8","author":"Lin","year":"2017","journal-title":"World J. Gastrointest. Pharmacol. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Furfaro, L.L., Payne, M.S., and Chang, B.J. (2018). Bacteriophage therapy: Clinical trials and regulatory hurdles. Front. Cell. Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00376"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"66","DOI":"10.4161\/bact.1.2.15845","article-title":"Phage treatment of human infections","volume":"1","author":"Abedon","year":"2011","journal-title":"Bacteriophage"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hasan, M., and Ahn, J. (2022). Evolutionary dynamics between phages and bacteria as a possible approach for designing effective phage therapies against antibiotic-resistant bacteria. Antibiotics, 11.","DOI":"10.3390\/antibiotics11070915"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Howard-Varona, C., Vik, D.R., Solonenko, N.E., Li, Y.F., Gazitua, M.C., Chittick, L., Samiec, J.K., Jensen, A.E., Anderson, P., and Howard-Varona, A. (2018). Fighting fire with fire: Phage potential for the treatment of E. coli O157 infection. Antibiotics, 7.","DOI":"10.3390\/antibiotics7040101"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Cho, E., Kim, J., Won, T., Ryu, S., and Jeon, B. (2025). Virulent bacteriophages for controlling Shiga toxin-producing Escherichia coli (STEC) without inducing toxin production. J. Infect. Dis., jiaf035.","DOI":"10.1093\/infdis\/jiaf035"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s10096-023-04638-1","article-title":"A systematic review of the use of bacteriophages for in vitro biofilm control","volume":"42","author":"Meneses","year":"2023","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Park, S.Y., Kwon, H., Kim, S.G., Park, S.C., Kim, J.H., and Seo, S. (2023). Characterization of two lytic bacteriophages, infecting Streptococcus bovis\/equinus complex (SBSEC) from Korean ruminant. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-36306-x"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1743-422X-3-30","article-title":"Genetic diversity among five T4-like bacteriophages","volume":"3","author":"Nolan","year":"2006","journal-title":"Virol. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1111\/1462-2920.14590","article-title":"Riding the wave of genomics to investigate aquatic coliphage diversity and activity","volume":"21","author":"Michniewski","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"8206","DOI":"10.1128\/JB.00838-07","article-title":"Genome analysis of phage JS98 defines a fourth major subgroup of T4-like phages in Escherichia coli","volume":"189","author":"Zuber","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3354\/dao03622","article-title":"Isolation, characterization and biocontrol efficacy of a T4-like phage virulent to multidrug-resistant Enterobacter hormaechei","volume":"147","author":"Lin","year":"2021","journal-title":"Dis. Aquat. Organ."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Liao, Y.T., Zhang, Y., Salvador, A., Harden, L.A., and Wu, V.C.H. (2022). Characterization of a T4-like bacteriophage vB_EcoM-Sa45lw as a potential biocontrol agent for Shiga toxin-producing Escherichia coli O45 contaminated on mung bean seeds. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.02220-21"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s00705-022-05694-2","article-title":"Abolishment of morphology-based taxa and change to binomial species names: 2022 taxonomy update of the ICTV bacterial viruses subcommittee","volume":"168","author":"Turner","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Park, E.J., Lee, S., Na, J.B., Kim, Y.B., Lee, K.M., Park, S.Y., and Kim, J.H. (2025). Characterization of Broad Spectrum Bacteriophage vB ESM-pEJ01 and Its Antimicrobial Efficacy Against Shiga Toxin-Producing Escherichia coli in Green Juice. Microorganisms, 13.","DOI":"10.3390\/microorganisms13010103"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Park, S.Y., Lee, K., Cho, Y., Lim, S.R., Kwon, H., Han, J.E., and Kim, J.H. (2020). Emergence of third-generation cephalosporin-resistant Morganella morganii in a captive breeding dolphin in South Korea. Animals, 10.","DOI":"10.3390\/ani10112052"},{"key":"ref_32","unstructured":"(2024). Performance Standards for Antimicrobial Susceptibility Testing (34th ed., CLSI supplement M100). Standard No. CLSI M100."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kwon, H., Park, S.Y., Kim, M.S., Kim, S.G., Park, S.C., and Kim, J.H. (2022). Characterization of a lytic bacteriophage vB_SurP-PSU3 infecting Staphylococcus ureilyticus and its efficacy against biofilm. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.925866"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kabwe, M., Brown, T.L., Dashper, S., Speirs, L., Ku, H., Petrovski, S., and Tucci, J. (2019). Genomic, morphological and functional characterisation of novel bacteriophage FNU1 capable of disrupting Fusobacterium nucleatum biofilms. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-45549-6"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1093\/bioinformatics\/17.7.646","article-title":"Evaluation of methods for the prediction of membrane spanning regions","volume":"17","author":"Croning","year":"2001","journal-title":"Bioinformatics."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/s41587-021-01156-3","article-title":"SignalP 6.0 predicts all five types of signal peptides using protein language models","volume":"40","author":"Teufel","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Garneau, J.R., Depardieu, F., Fortier, L.C., Bikard, D., and Monot, M. (2017). PhageTerm: A tool for fast and accurate determination of phage termini and packaging mechanism using next generation sequencing data. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-07910-5"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9077","DOI":"10.1093\/nar\/gkab688","article-title":"tRNAscan-SE 2.0: Improved detection and functional classification of transfer RNA genes","volume":"49","author":"Chan","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Magill, D.J., and Skvortsov, T.A. (2023). DePolymerase Predictor (DePP): A machine learning tool for the targeted identification of phage depolymerases. BMC Bioinform., 24.","DOI":"10.1186\/s12859-023-05341-w"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"D690","DOI":"10.1093\/nar\/gkac920","article-title":"CARD 2023: Expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database","volume":"51","author":"Bonin","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"D912","DOI":"10.1093\/nar\/gkab1107","article-title":"VFDB 2022: A general classification scheme for bacterial virulence factors","volume":"50","author":"Liu","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"W484","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: In-depth characterization and visualization of bacterial genomes","volume":"51","author":"Grant","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3396","DOI":"10.1093\/bioinformatics\/btx440","article-title":"VICTOR: Genome-based phylogeny and classification of prokaryotic viruses","volume":"33","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Moraru, C., Varsani, A., and Kropinski, A.M. (2020). VIRIDIC\u2014A novel tool to calculate the intergenomic similarities of prokaryote-infecting viruses. Viruses, 12.","DOI":"10.1101\/2020.07.05.188268"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: A genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1093\/bioinformatics\/btx157","article-title":"ViPTree: The viral proteomic tree server","volume":"33","author":"Nishimura","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1046\/j.1365-2672.2000.01211.x","article-title":"An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5\u03b1 and EQ1","volume":"89","author":"Chart","year":"2000","journal-title":"J. Appl. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5569","DOI":"10.1111\/1462-2920.15714","article-title":"Bacteriophage isolated from non-target bacteria demonstrates broad host range infectivity against multidrug-resistant bacteria","volume":"23","author":"Ngiam","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Dalmasso, M., Strain, R., Neve, H., Franz, C.M.A.P., Cousin, F.J., Ross, R.P., and Hill, C. (2016). Three new Escherichia coli phages from the human gut show promising potential for phage therapy. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0156773"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4756","DOI":"10.1111\/ijfs.15070","article-title":"A bacteriophage JN02 infecting multidrug-resistant Shiga toxin-producing Escherichia coli: Isolation, characterisation and application as a biocontrol agent in foods","volume":"56","author":"Li","year":"2021","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s12223-011-0039-8","article-title":"The influence of external factors on bacteriophages\u2014Review","volume":"56","year":"2011","journal-title":"Folia Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Wang, C., Hang, H., Zhou, S., Niu, Y.D., Du, H., Stanford, K., and McAllister, T.A. (2020). Bacteriophage biocontrol of Shiga toxigenic Escherichia coli (STEC) O145 biofilms on stainless steel reduces the contamination of beef. Food Microbiol., 92.","DOI":"10.1016\/j.fm.2020.103572"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.12688\/f1000research.159559.1","article-title":"Isolation and characterization of a novel bacteriophage as a biological control agent against multidrug-resistant Escherichia coli in compost and agricultural irrigation water","volume":"13","author":"Amarillas","year":"2024","journal-title":"F1000Research"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e85183","DOI":"10.7554\/eLife.85183","article-title":"Phage tRNAs evade tRNA-targeting host defenses through anticodon loop mutations","volume":"12","author":"Costa","year":"2023","journal-title":"eLife"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e1219441","DOI":"10.1080\/21597081.2016.1219441","article-title":"Testing hypotheses for the presence of tRNA genes in mycobacteriophage genomes","volume":"6","author":"Delesalle","year":"2016","journal-title":"Bacteriophage"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1101\/gr.6649807","article-title":"Causes for the intriguing presence of tRNAs in phages","volume":"17","author":"Vergassola","year":"2007","journal-title":"Genome Res."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Sun, L., You, J., Li, D., Zhang, Z., Qin, X., Pang, W., Li, P., Han, Q., Li, Y., and Huang, Z. (2023). Variants of a putative baseplate wedge protein extend the host range of Pseudomonas phage K8. Microbiome, 11.","DOI":"10.1186\/s40168-022-01459-w"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e00423-21","DOI":"10.1128\/AEM.00423-21","article-title":"Manipulating interactions between T4 phage long tail fibers and bacterial outer membrane protein C residues defines host specificity","volume":"87","author":"Trotereau","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1007\/s00253-015-7247-0","article-title":"Bacteriophage-encoded depolymerases: Their diversity and biotechnological applications","volume":"100","author":"Pires","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2273567","DOI":"10.1080\/21505594.2023.2273567","article-title":"Potential of phage depolymerase for the treatment of bacterial biofilms","volume":"14","author":"Guo","year":"2023","journal-title":"Virulence"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Islam, M.Z., Fokine, A., Mahalingam, M., Zhang, Z., Garcia-Doval, C., van Raaij, M.J., Rossmann, M.G., and Rao, V.B. (2019). Molecular anatomy of the receptor binding module of a bacteriophage long tail fiber. PLoS Pathog., 15.","DOI":"10.1371\/journal.ppat.1008193"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e01576-17","DOI":"10.1128\/AEM.01576-17","article-title":"Alterations in gp37 expand the host range of a T4-like phage","volume":"83","author":"Chen","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.cell.2019.09.015","article-title":"Engineering phage host-range and suppressing bacterial resistance through phage tail fiber mutagenesis","volume":"179","author":"Yehl","year":"2019","journal-title":"Cell"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Taslem Mourosi, J., Awe, A., Guo, W., Batra, H., Ganesh, H., Wu, X., and Zhu, J. (2022). Understanding bacteriophage tail fiber interaction with host surface receptor: The key \u201cblueprint\u201d for reprogramming phage host range. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232012146"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/11\/1501\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T05:28:55Z","timestamp":1763184535000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/11\/1501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,13]]},"references-count":67,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["v17111501"],"URL":"https:\/\/doi.org\/10.3390\/v17111501","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,13]]}}}