{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T04:50:09Z","timestamp":1764996609957,"version":"3.45.0"},"reference-count":35,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>African swine fever virus (ASFV) is a high-consequence pathogen that causes African swine fever (ASF), for which mortality rates can reach 90\u2013100%, with death typically occurring within 14 days. ASF is currently a highly contagious pandemic disease responsible for extensive losses in pig production in multiple affected countries suffering from extended outbreaks. While a limited number of vaccines to prevent ASF are in use in south-east Asia, vaccines are not widely available, are only effective against highly homologous strains of ASFV, and must be used prior to an outbreak on a farm. Currently, there is no treatment for ASF and culling affected farms is the only response to outbreaks on farms to try and prevent spreading. CRISPR\/Cas systems evolved as an adaptive immune response in bacteria and archaea that function by cleaving and disrupting the genomes of invading bacteriophage pathogens. CRISPR technology has since been leveraged into an array of endonuclease-based systems used for nucleic acid detection, targeting, genomic cleavage, and gene editing, making them particularly well-suited for development as sequence-specific therapeutic modalities. The programmability of CRISPR-based therapeutics offers a compelling new way to rapidly and specifically target pathogenic viral genomes simply by using different targeting guide RNAs (gRNA) as an adaptable antiviral modality. Here, we demonstrate for the first time a specific CRISPR\/Cas9 multiplexed gRNA system that targets the African swine fever viral genome, resulting in sequence-specific cleavage, leading to the reduction in the viral load in infected animals, and subsequent recovery from an otherwise lethal dose of ASFV. Moreover, animals that recovered had protective immunity to subsequent homologous ASFV infection.<\/jats:p>","DOI":"10.3390\/v17111504","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T16:46:22Z","timestamp":1763138782000},"page":"1504","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The First CRISPR-Based Therapeutic (SL_1.52) for African Swine Fever Is Effective in Swine"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4211-579X","authenticated-orcid":false,"given":"Naveen","family":"Verma","sequence":"first","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison","family":"O\u2019Mahony","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roky","family":"Mohammad","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dylan","family":"Keiser","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig W.","family":"Mosman","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deric","family":"Holden","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin","family":"Starr","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared","family":"Bauer","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley","family":"Bauer","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roypim","family":"Suntisukwattana","sequence":"additional","affiliation":[{"name":"Swine Viral Evolution and Vaccine Development Research Unit, Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henry Dunant Road, Pathumwan, Bangkok 10330, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waranya","family":"Atthaapa","sequence":"additional","affiliation":[{"name":"Swine Viral Evolution and Vaccine Development Research Unit, Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henry Dunant Road, Pathumwan, Bangkok 10330, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angkana","family":"Tantituvanont","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutic and Industrial Pharmacies, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dachrit","family":"Nilubol","sequence":"additional","affiliation":[{"name":"Swine Viral Evolution and Vaccine Development Research Unit, Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henry Dunant Road, Pathumwan, Bangkok 10330, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7894-0233","authenticated-orcid":false,"given":"Douglas P.","family":"Gladue","sequence":"additional","affiliation":[{"name":"Seek Labs, 350 W 800 N, Salt Lake City, UT 84103, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2683","DOI":"10.1098\/rstb.2009.0098","article-title":"African swine fever: How can global spread be prevented?","volume":"364","author":"Costard","year":"2009","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0368-1742(21)80031-4","article-title":"On a form of swine fever occuring in British East Africa (Kenya Colony)","volume":"34","author":"Montgomery","year":"1921","journal-title":"J. Comp. Pathol. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"599","DOI":"10.3201\/eid1704.101283","article-title":"Genomic analysis of highly virulent Georgia 2007\/1 isolate of African swine fever virus","volume":"17","author":"Chapman","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3018","DOI":"10.1111\/tbed.14341","article-title":"African swine fever in the Dominican Republic","volume":"68","author":"Gonzales","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Jean-Pierre, R.P., Hagerman, A.D., and Rich, K.M. (2022). An analysis of African Swine Fever consequences on rural economies and smallholder swine producers in Haiti. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.960344"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/978-3-540-68618-7_2","article-title":"African Swine Fever Virus","volume":"Volume 328","author":"Etten","year":"2009","journal-title":"Lesser Known Large dsDNA Viruses"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Spinard, E., Azzinaro, P., Rai, A., Espinoza, N., Ramirez-Medina, E., Valladares, A., Borca, M.V., and Gladue, D.P. (2022). Complete Structural Predictions of the Proteome of African Swine Fever Virus Strain Georgia 2007. Microbiol. Resour. Announc., 11.","DOI":"10.1128\/mra.00881-22"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Gladue, D.P., and Borca, M.V. (2022). Recombinant ASF Live Attenuated Virus Strains as Experimental Vaccine Candidates. Viruses, 14.","DOI":"10.3390\/v14050878"},{"key":"ref_9","first-page":"10","article-title":"Rapid Sequence-Based Characterization of African Swine Fever Virus by Use of the Oxford Nanopore MinION Sequence Sensing Device and a Companion Analysis Software Tool","volume":"58","author":"Grau","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0168-1702(91)90080-F","article-title":"Repetitive nucleotide sequencing of a dispensable DNA segment in a clonal population of African swine fever virus","volume":"20","author":"Yanez","year":"1991","journal-title":"Virus Res."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Shi, C., Wang, Q., Liu, Y., Wang, S., Zhang, Y., Liu, C., Hu, Y., Zheng, D., Sun, C., and Song, F. (2024). Generation of High-Quality African Swine Fever Virus Complete Genome from Field Samples by Next-Generation Sequencing. Viruses, 16.","DOI":"10.3390\/v16020312"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Spinard, E., Dinhobl, M., Erdelyan, C.N.G., O\u2019Dwyer, J., Fenster, J., Birtley, H., Tesler, N., Calvelage, S., Leijon, M., and Steinaa, L. (2024). A Standardized Pipeline for Assembly and Annotation of African Swine Fever Virus Genome. Viruses, 16.","DOI":"10.3390\/v16081293"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Dinhobl, M., Spinard, E., Tesler, N., Birtley, H., Signore, A., Ambagala, A., Masembe, C., Borca, M.V., and Gladue, D.P. (2023). Reclassification of ASFV into 7 Biotypes Using Unsupervised Machine Learning. Viruses, 16.","DOI":"10.3390\/v16010067"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/JVI.01058-17","article-title":"BA71DeltaCD2: A New Recombinant Live Attenuated African Swine Fever Virus with Cross-Protective Capabilities","volume":"91","author":"Monteagudo","year":"2017","journal-title":"J. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Diep, N.V., Duc, N.V., Ngoc, N.T., Dang, V.X., Tiep, T.N., Nguyen, V.D., Than, T.T., Maydaniuk, D., Goonewardene, K., and Ambagala, A. (2024). Genotype II Live-Attenuated ASFV Vaccine Strains Unable to Completely Protect Pigs against the Emerging Recombinant ASFV Genotype I\/II Strain in Vietnam. Vaccines, 12.","DOI":"10.20944\/preprints202408.1438.v1"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nature10886","article-title":"RNA-guided genetic silencing systems in bacteria and archaea","volume":"482","author":"Wiedenheft","year":"2012","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.mib.2011.03.005","article-title":"CRISPR-based adaptive immune systems","volume":"14","author":"Terns","year":"2011","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1146\/annurev-genet-110410-132430","article-title":"CRISPR-Cas systems in bacteria and archaea: Versatile small RNAs for adaptive defense and regulation","volume":"45","author":"Bhaya","year":"2011","journal-title":"Annu. Rev. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1093\/clinchem\/hvae038","article-title":"Approval of the First CRISPR-Cas9 Gene Editing Therapy for Sickle Cell Disease","volume":"70","author":"Campbell","year":"2024","journal-title":"Clin. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/545277a","article-title":"Geneticists enlist engineered virus and CRISPR to battle citrus disease","volume":"545","author":"Ledford","year":"2017","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1007\/s11262-024-02105-3","article-title":"Tackling hepatitis B Virus with CRISPR\/Cas9: Advances, challenges, and delivery strategies","volume":"60","author":"Nair","year":"2024","journal-title":"Virus Genes"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Sasaki, S., Ogawa, H., Katoh, H., and Honda, T. (2024). Suppression of Borna Disease Virus Replication during Its Persistent Infection Using the CRISPR\/Cas13b System. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25063523"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10717","DOI":"10.1093\/nar\/gkae739","article-title":"Cryo-EM structure of DNA polymerase of African swine fever virus","volume":"52","author":"Kuai","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e2978","DOI":"10.21769\/BioProtoc.2978","article-title":"CRISPR\/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus","volume":"8","author":"Borca","year":"2018","journal-title":"Bio-Protoc."},{"key":"ref_26","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Hyg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1038\/nbt.3437","article-title":"Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9","volume":"34","author":"Doench","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zhu, X., Ricci-Tam, C., Hager, E.R., and Sgro, A.E. (2023). Self-cleaving peptides for expression of multiple genes in Dictyostelium discoideum. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0281211"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1016\/j.ymthe.2018.08.021","article-title":"Engineering the Direct Repeat Sequence of crRNA for Optimization of FnCpf1-Mediated Genome Editing in Human Cells","volume":"26","author":"Lin","year":"2018","journal-title":"Mol. Ther."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"120586","DOI":"10.1016\/j.ijpharm.2021.120586","article-title":"mRNA-lipid nanoparticle COVID-19 vaccines: Structure and stability","volume":"601","author":"Schoenmaker","year":"2021","journal-title":"Int. J. Pharm."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"197667","DOI":"10.1016\/j.virusres.2019.197667","article-title":"African swine fever virus replication events and cell nucleus: New insights and perspectives","volume":"270","author":"Simoes","year":"2019","journal-title":"Virus Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1128\/JVI.78.8.4299-4313.2004","article-title":"African swine fever virus structural protein p54 is essential for the recruitment of envelope precursors to assembly sites","volume":"78","author":"Rodriguez","year":"2004","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Hubner, A., Petersen, B., Keil, G.M., Niemann, H., Mettenleiter, T.C., and Fuchs, W. (2018). Efficient inhibition of African swine fever virus replication by CRISPR\/Cas9 targeting of the viral p30 gene (CP204L). Sci. Rep., 8.","DOI":"10.1038\/s41598-018-19626-1"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8263","DOI":"10.1128\/JVI.00704-11","article-title":"Disruption of nuclear organization during the initial phase of African swine fever virus infection","volume":"85","author":"Ballester","year":"2011","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Zheng, Z., Xu, L., Gao, Y., Dou, H., Zhou, Y., Feng, X., He, X., Tian, Z., Song, L., and Mo, G. (2024). Testing multiplexed anti-ASFV CRISPR-Cas9 in reducing African swine fever virus. Microbiol. Spectr., 12.","DOI":"10.1128\/spectrum.02164-23"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/11\/1504\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T10:06:53Z","timestamp":1763460413000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/11\/1504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,14]]},"references-count":35,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["v17111504"],"URL":"https:\/\/doi.org\/10.3390\/v17111504","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2025,11,14]]}}}