{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:11:11Z","timestamp":1783401071287,"version":"3.54.6"},"reference-count":35,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province","award":["2024KJ14"],"award-info":[{"award-number":["2024KJ14"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2021YFD1800100"],"award-info":[{"award-number":["2021YFD1800100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds for the Central Universities, China Agriculture Research System of Ministry of Finance and Ministry of Agriculture and Rural Affairs","award":["CARS-35"],"award-info":[{"award-number":["CARS-35"]}]},{"name":"Project of National Center of Technology Innovation for Pigs","award":["NCTIP-XD\/C03"],"award-info":[{"award-number":["NCTIP-XD\/C03"]}]},{"name":"Innovation Program of Chinese Academy of Agricultural Sciences","award":["CAAS-CSLPDCP-202302"],"award-info":[{"award-number":["CAAS-CSLPDCP-202302"]}]},{"name":"Innovation Program of Chinese Academy of Agricultural Sciences","award":["CAAS-ASTIP-2024-LVRI"],"award-info":[{"award-number":["CAAS-ASTIP-2024-LVRI"]}]},{"DOI":"10.13039\/501100018554","name":"Science and Technology Program of Gansu Province","doi-asserted-by":"publisher","award":["24JRRA01"],"award-info":[{"award-number":["24JRRA01"]}],"id":[{"id":"10.13039\/501100018554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>African swine fever virus (ASFV) poses a significant threat to the global pig industry due to high mortality rates and complex genetic variation. Live attenuated vaccines (LAVs) provide protection against ASFV. Previously, MGF505-2R was identified as a potent inhibitor of innate immunity in vitro. This study evaluates the pathogenicity of a recombinant Eurasian genotype II strain with the MGF505-2R gene deleted (ASFV-\u0394MGF505-2R) in piglets. Twelve five-week-old crossbred piglets were divided into two groups, with one group of eight piglets inoculated with ASFV-\u0394MGF505-2R (n = 8) and the other group of four piglets inoculated with the same dose of parental ASFV CN\/GS 2018 (n = 4). Clinical symptoms, viral loads, and immune responses were monitored over 30 days. ASFV-\u0394MGF505-2R-inoculated piglets exhibited transient fever and low viremia only in the beginning of the challenge, while the control group developed high levels of viremia and hyperthermia at day 2 and 8 post-challenge, respectively. Meanwhile, the control group demonstrated more severe post-mortem signs and immuno-histochemistry injury when compared to the \u0394MGF505-2R group. ELISA analysis displayed higher levels of IFN-\u03b2 and IL-1\u03b2 in the \u0394MGF505-2R group, further solidating the immunosuppressive role of MGF505-2R. All ASFV-\u0394MGF505-2R-inoculated piglets developed high titers of ASFV-specific P30 antibodies at 10 days post-challenge. These findings rationalized the potential of ASFV-\u0394MGF505-2R as a live attenuated candidate for ASF infection.<\/jats:p>","DOI":"10.3390\/v17121565","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T13:03:02Z","timestamp":1764594182000},"page":"1565","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of the Pathogenicity of Highly Virulent Eurasian Genotype II African Swine Fever Virus with MGF505-2R Gene Deletion in Piglets"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2535-0002","authenticated-orcid":false,"given":"Fan","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaguo","family":"Huang","sequence":"additional","affiliation":[{"name":"Ecology and Agriculture College, Sichuan Minzu College, Kangding 626001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Dang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengwang","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jijun","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6850-1379","authenticated-orcid":false,"given":"Haixue","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Spinard, E., O\u2019Donnell, V., Vuono, E., Rai, A., Davis, C., Ramirez-Medina, E., Espinoza, N., Valladares, A., Borca, M.V., and Gladue, D.P. 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Res."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/12\/1565\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T05:16:42Z","timestamp":1764652602000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/12\/1565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,29]]},"references-count":35,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["v17121565"],"URL":"https:\/\/doi.org\/10.3390\/v17121565","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,29]]}}}