{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T21:00:44Z","timestamp":1780520444042,"version":"3.54.1"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Postgraduate Program in Health Sciences"},{"name":"Parreiras Horta Health Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The diversity of clinical presentations and outcomes of COVID-19 suggests the influence of host-intrinsic factors that modulate the infectious process. Therefore, a study was conducted with professionals from a hospital in the state of Sergipe, in the Northeast region of Brazil, aiming to identify in this population the effect of rs12329760 and rs2070788, SNPs of the TMPRSS2 enzyme that facilitates the infectious process. Recruitment of the 363 participants followed a non-probabilistic method using a QR code that led to the Informed Consent Form (ICF) and a clinical\u2013epidemiological questionnaire based on self-reported information on the number of positive tests, the presence\/absence of symptoms, and severity. Buccal epithelial cells were collected, DNA was extracted using a silica column, and SNP amplification was performed by qPCR. The data were processed using PSPP software, using chi-squared tests for associations in three statistical genetic models (additive, dominant, and recessive). The results showed that, in this population, rs12329760 did not influence any of the outcomes, while rs2070788 was significant in both the additive and recessive models. The action of the G allele is evident in the most severe cases, and it is associated with increased TMPRSS2 expression and potentially increased viral entry efficiency.<\/jats:p>","DOI":"10.3390\/v17121587","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T10:50:38Z","timestamp":1764931838000},"page":"1587","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic Association Between TMPRSS2 Polymorphisms and COVID-19 Severity in Brazilian Health Workers"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3792-7136","authenticated-orcid":false,"given":"Alysson Fellipe Costa","family":"Telles","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade da Universidade Federal de Sergipe, Aracaju 49107-230, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bearli Souza","family":"Menezes Junior","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Sa\u00fade Parreiras Horta\u2014Lacen SE, Aracaju 49020-590, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7993-8664","authenticated-orcid":false,"given":"Cliomar Alves","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Sa\u00fade Parreiras Horta\u2014Lacen SE, Aracaju 49020-590, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rosa Melo","family":"Alves","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade da Universidade Federal de Sergipe, Aracaju 49107-230, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila Oliveira Carvalho","family":"Sena","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade da Universidade Federal de Sergipe, Aracaju 49107-230, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9070-2302","authenticated-orcid":false,"given":"Rosana","family":"Cipolotti","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade da Universidade Federal de Sergipe, Aracaju 49107-230, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100443","DOI":"10.1016\/j.imu.2020.100443","article-title":"Understand variability of COVID-19 through population and tissue variations in expression of SARS-CoV-2 host genes","volume":"21","author":"Chen","year":"2020","journal-title":"Inform. Med. Unlocked"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hou, Y., Zhao, J., Martin, W., Kallianpur, A., Chung, M.K., Jehi, L., Sharifi, N., Erzurum, S., Eng, C., and Cheng, F. (2020). New insights into genetic susceptibility of COVID-19: An ACE2 and TMPRSS2 polymorphism analysis. BMC Med., 18.","DOI":"10.1186\/s12916-020-01673-z"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/imr.12897","article-title":"The role of host genetics in the immune response to SARS-CoV-2 and COVID-19 susceptibility and severity","volume":"296","author":"Ovsyannikova","year":"2020","journal-title":"Immunol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"104846","DOI":"10.1016\/j.meegid.2021.104846","article-title":"Genetic polymorphisms as multi-biomarkers in severe acute respiratory syndrome (SARS) by coronavirus infection: A systematic review of candidate gene association studies","volume":"93","author":"Ferreira","year":"2021","journal-title":"Infect. Genet. Evol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e31810111812","DOI":"10.33448\/rsd-v10i1.11812","article-title":"Influ\u00eancia da suscetibilidade gen\u00e9tica na incid\u00eancia e mortalidade de COVID-19 (SARS-CoV-2)","volume":"10","author":"Galisa","year":"2021","journal-title":"Res. Soc. Dev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"146790","DOI":"10.1016\/j.gene.2022.146790","article-title":"Systematic review and meta-analysis of human genetic variants contributing to COVID-19 susceptibility and severity","volume":"844","author":"Gupta","year":"2022","journal-title":"Gene"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"91868","DOI":"10.34117\/bjdv7n9-379","article-title":"Polimorfismo da ACE2 e a Influ\u00eancia na Suscetibilidade frente \u00e0 COVID-19\/ACE2 Polymorphism and the Influence on Susceptibility to COVID-19","volume":"7","author":"Reser","year":"2021","journal-title":"Braz. J. Dev."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"de Andrade, C.C., Silva, A.T.P., Vasconcelos, L.R.S., Oliveira, P.R.S., de Souza, C.D.F., da Costa Armstrong, A., and do Carmo, R.F. (2022). A Polymorphism in the TMPRSS2 Gene Increases the Risk of Death in Older Patients Hospitalized with COVID-19. Viruses, 14.","DOI":"10.3390\/v14112557"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sch\u00f6nfelder, K., Breuckmann, K., Elsner, C., Dittmer, U., Fistera, D., Herbstreit, F., Risse, J., Schmidt, K., Sutharsan, S., and Taube, C. (2021). Transmembrane serine protease 2 Polymorphisms and Susceptibility to Severe Acute Respiratory Syndrome Coronavirus Type 2 Infection: A German Case-Control Study. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.667231"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1002\/jmv.26319","article-title":"ACE2, TMPRSS2, and Furin variants and SARS-CoV-2 infection in Madrid, Spain","volume":"93","author":"Pytel","year":"2021","journal-title":"J. Med. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kehdy, F.S., Pita-Oliveira, M., Scudeler, M.M., Torres-Loureiro, S., Zolini, C., Moreira, R., Michelin, L.A., Alvim, I., Silva-Carvalho, C., and Furlan, V.C. (2021). Human-SARS-CoV-2 interactome and human genetic diversity: TMPRSS2-rs2070788, associated with severe influenza, and its population genetics caveats in Native Americans. Genet. Mol. Biol., 44.","DOI":"10.1590\/1678-4685-gmb-2020-0484"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zarubin, A., Stepanov, V., Markov, A., Kolesnikov, N., Marusin, A., Khitrinskaya, I., Swarovskaya, M., Litvinov, S., Ekomasova, N., and Dzhaubermezov, M. (2020). Structural Variability, Expression Profile, and Pharmacogenetic Properties of TMPRSS2 Gene as a Potential Target for COVID-19 Therapy. Genes, 12.","DOI":"10.1101\/2020.06.20.156224"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3343\/alm.2021.41.2.129","article-title":"Role of Genetic Variants and Gene Expression in the Susceptibility and Severity of COVID-19","volume":"41","author":"Choudhary","year":"2021","journal-title":"Ann. Lab. Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Suh, S., Lee, S., Gym, H., Yoon, S., Park, S., Cha, J., Kwon, D.-H., Yang, Y., and Jee, S.H. (2022). A systematic review on papers that study on Single Nucleotide Polymorphism that affects coronavirus 2019 severity. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07034-w"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"39","DOI":"10.17219\/acem\/163409","article-title":"The relationship between the clinical course of SARS-CoV-2 infections and ACE2 and TMPRSS2 expression and polymorphisms","volume":"33","author":"Tug","year":"2023","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_16","unstructured":"(2025, June 17). Brazil. Protocolo de Manejo Cl\u00ednico do Coronav\u00edrus (COVID COVID-19) na Aten\u00e7\u00e3o Prim\u00e1ria \u00e0 Sa\u00fade, Available online: https:\/\/saude.rs.gov.br\/upload\/arquivos\/202004\/14140606-4-ms-protocolomanejo-aps-ver07abril.pdf."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s12985-024-02298-x","article-title":"Genetic association of ACE2 and TMPRSS2 polymorphisms with COVID-19 severity; a single centre study from Egypt","volume":"21","author":"Elnagdy","year":"2024","journal-title":"Virol. J."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-G\u00f3mez, L.E., Herrera-L\u00f3pez, B., Martinez-Armenta, C., Ortega-Pe\u00f1a, S., Camacho-Rea, M.d.C., Suarez-Ahedo, C., V\u00e1zquez-C\u00e1rdenas, P., Vargas-Alarc\u00f3n, G., Rojas-Velasco, G., and Fragoso, J.M. (2022). ACE and ACE2 Gene Variants Are Associated With Severe Outcomes of COVID-19 in Men. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.812940"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"107067","DOI":"10.1016\/j.ijid.2024.107067","article-title":"Gene variants rs5182, rs2074192, and rs4343 in the renin-angiotensin-aldosterone system are associated with symptom severity, higher odds of hospitalization, and death in COVID-19","volume":"144","author":"Lara","year":"2024","journal-title":"Int. J. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"34697","DOI":"10.34117\/bjdv7n4-093","article-title":"Adapta\u00e7\u00e3o de um m\u00e9todo de extra\u00e7\u00e3o de DNA a partir de c\u00e9lulas da mucosa bucal baseado em par\u00e2metros de qualidade \/ Adaptation of a DNA extraction method from buccal mucosa cells based on quality parameters","volume":"7","author":"Araujo","year":"2021","journal-title":"Braz. J. Dev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1007\/s42770-023-00966-8","article-title":"Seroprevalence of SARS-CoV-2 in hospital workers in the southern region of Minas Gerais state in Brazil: An analysis of the pre-vaccine period","volume":"54","author":"Caixeta","year":"2023","journal-title":"Braz. J. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Cegolon, L., Mauro, M., Sansone, D., Tassinari, A., Gobba, F.M., Modenese, A., Casolari, L., Liviero, F., Pavanello, S., and Scapellato, M.L. (2023). A Multi-Center Study Investigating Long COVID-19 in Healthcare Workers from North-Eastern Italy: Prevalence, Risk Factors and the Impact of Pre-Existing Humoral Immunity\u2014ORCHESTRA Project. Vaccines, 11.","DOI":"10.3390\/vaccines11121769"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1093\/infdis\/jiv246","article-title":"Identification of TMPRSS2 as a Susceptibility Gene for Severe 2009 Pandemic A(H1N1) Influenza and A(H7N9) Influenza","volume":"212","author":"Cheng","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e2853","DOI":"10.1093\/cid\/ciaa1471","article-title":"SARS-CoV-2 seroprevalence survey among 18,000 healthcare and administrative personnel at hospitals, pre-hospital services, and specialist practitioners in the Central Denmark Region","volume":"73","author":"Jespersen","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Kr\u00f6\u00f6nstr\u00f6m, L.A., Krause, J., Larsson, S.B., Sigstr\u00f6m, R., and Sunnerhagen, K.S. (2022). Long-term self-reported health and disability after COVID-19 in public employees. BMC Public Heal., 22.","DOI":"10.1186\/s12889-022-14820-3"},{"key":"ref_26","unstructured":"Instituto Brasileiro de Geografia e Estat\u00edstica (IBGE) (2025, June 23). Censo 2021, Available online: https:\/\/www.ibge.gov.br\/."},{"key":"ref_27","unstructured":"Instituto Brasileiro de Geografia e Estat\u00edstica (2025, June 23). Sergipe Cidades e Estados. IBGE 2023, Available online: https:\/\/www.ibge.gov.br\/cidades-e-estados\/se.html."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Abdelsattar, S., Kasemy, Z.A., Ewida, S.F., Abo-Elsoud, R.A.A., Zytoon, A.A., Abdelaal, G.A., Abdelgawad, A.S., Khalil, F.O., and Kamel, H.F.M. (2022). ACE2 and TMPRSS2 SNPs as Determinants of Susceptibility to, and Severity of, a COVID-19 Infection. Br. J. Biomed Sci., 79.","DOI":"10.3389\/bjbs.2021.10238"},{"key":"ref_29","first-page":"103333","article-title":"A common TMPRSS2 variant has a protective effect against severe COVID-19","volume":"70","author":"David","year":"2022","journal-title":"Curr. Res. Transl. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105206","DOI":"10.1016\/j.meegid.2022.105206","article-title":"Genetic association of TMPRSS2 rs2070788 polymorphism with COVID-19 case fatality rate among Indian populations","volume":"98","author":"Pandey","year":"2022","journal-title":"Infect. Genet. Evol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/12\/1587\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T11:11:57Z","timestamp":1764933117000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/17\/12\/1587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,5]]},"references-count":30,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["v17121587"],"URL":"https:\/\/doi.org\/10.3390\/v17121587","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,5]]}}}