{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:29:32Z","timestamp":1782577772810,"version":"3.54.5"},"reference-count":20,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T00:00:00Z","timestamp":1766620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CNPq scholarship through the Institutional Scientific Initiation Scholarship Program of UNESP","award":["PIBIC 2023\/2024"],"award-info":[{"award-number":["PIBIC 2023\/2024"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior-Brasil (CAPES)-Finance Code 001"},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/22251-0"],"award-info":[{"award-number":["2024\/22251-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>This study describes an optimized plastic surface-based capsid protein adsorption\/capturing method for detection of cowpea mild mottle virus (CPMMV) adapted from the direct antigen-capture method reported for the extraction of rose rosette virus (RRV) and other direct virus capturing attempts. Briefly, the method starts with sap incubation, removal of unbound residual tissue and inhibitors by washing, and the viral RNA release using nuclease-free water and heat, in the presence of an RNase inhibitor. The protocol\u2019s efficiency was assessed across different pH conditions, RNaseOUT concentrations, and reverse-transcriptase choices, and its performance was compared with commercial RNA-extraction methods. Three hundred thirty-two positive samples for CPMMV were processed using the optimized protocol (PBS-T, pH 7.4; RNaseOUT at 0.5 U\/\u00b5L; and M-MLV reverse transcriptase). RT-PCR detection results were consistent with those obtained using the standard method. Cost estimates for tissue trapping indicate reductions of approximately 70% and 90% compared with the Qiagen RNeasy kit (Qiagen, Hilden, Germany) and the Bertheau method, respectively. The tissue-absorption protocol combines simplicity and low cost, making it particularly well suited for field diagnostics; by enabling rapid recovery of viral RNA without commercial kits and substantially reducing processing steps, it represents a practical, cost-effective alternative for routine CPMMV testing.<\/jats:p>","DOI":"10.3390\/v18010041","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T02:07:58Z","timestamp":1766714878000},"page":"41","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Rapid, Cost-Effective RNA Recovery of Cowpea Mild Mottle Virus (CPMMV) Directly from PCR Tubes Adsorption for Routine-Scale Detection in Soybean"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1233-419X","authenticated-orcid":false,"given":"Pedro Henrique","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Department of Plant Protection, S\u00e3o Paulo State University (UNESP), Botucatu, SP 18610-307, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5024-1801","authenticated-orcid":false,"given":"Marcos R.","family":"Ribeiro-Junior","sequence":"additional","affiliation":[{"name":"Institute for Biosecurity and Microbial Forensics (IBMF), Oklahoma State University (OSU), Stillwater, OK 74078, USA"},{"name":"Department of Entomology and Plant Pathology, Oklahoma State University (OSU), Stillwater, OK 74078, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1207-4341","authenticated-orcid":false,"given":"B\u00e1rbara R. R.","family":"Silveira","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, S\u00e3o Paulo State University (UNESP), Botucatu, SP 18610-307, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4112-8209","authenticated-orcid":false,"given":"Francisco M.","family":"Ochoa-Corona","sequence":"additional","affiliation":[{"name":"Institute for Biosecurity and Microbial Forensics (IBMF), Oklahoma State University (OSU), Stillwater, OK 74078, USA"},{"name":"Department of Entomology and Plant Pathology, Oklahoma State University (OSU), Stillwater, OK 74078, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7526-640X","authenticated-orcid":false,"given":"Renate","family":"Krause-Sakate","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, S\u00e3o Paulo State University (UNESP), Botucatu, SP 18610-307, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,25]]},"reference":[{"key":"ref_1","unstructured":"(2024, December 17). 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Inst."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/1\/41\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T06:29:19Z","timestamp":1766989759000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/1\/41"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,25]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["v18010041"],"URL":"https:\/\/doi.org\/10.3390\/v18010041","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,25]]}}}