{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T05:17:50Z","timestamp":1767331070537,"version":"3.48.0"},"reference-count":63,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Poxv\u00edrus","award":["406441\/2022-7"],"award-info":[{"award-number":["406441\/2022-7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The monkeypox virus (MPXV) is an emerging zoonotic pathogen responsible for mpox, a disease characterized by some smallpox-like symptoms, typically mild but occasionally fatal. The largest mpox recorded global outbreak began in May 2022, with over 162,000 cases across 140 countries. Herein, we have analyzed the intra-clonal diversity of MPXV obtained from a single skin lesion sample from a male patient (June 2022). Three viral clones were obtained following phenotypic evaluation of MPXV lysis plaque characteristics over a three-course infection in BSC-40 cells. Unlike the vaccinia virus Western Reserve (VACV-WR) strain, MPXV clones did not produce comet-like structures, suggesting reduced extracellular enveloped virus (EEV) morphotype release, which is associated with viral dissemination. Whole-genome sequencing and assembly identified subtle differences among clones. Comparative genomic analyses, including synteny and single nucleotide variation (SNV) calling, revealed intra-clonal differences and divergence from clade I and II references, although the variety of mutations found did not reveal possible variations at the protein level. Altogether, these findings suggest that although similar, it is possible that distinct MPXV variants may circulate together and can be found in a single exanthematous lesion.<\/jats:p>","DOI":"10.3390\/v18010062","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T16:34:23Z","timestamp":1767198863000},"page":"62","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unveiling Intra-Clonal Diversity of Monkeypox Virus from Brazil\u2019s First Outbreak Wave"],"prefix":"10.3390","volume":"18","author":[{"given":"Amanda St\u00e9phanie Arantes","family":"Witt","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7216-0738","authenticated-orcid":false,"given":"Jo\u00e3o Victor Rodrigues Pessoa","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0707-5588","authenticated-orcid":false,"given":"Izabela","family":"Mamede","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Bioqu\u00edmica, Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1471-0084","authenticated-orcid":false,"given":"Talita Emile Ribeiro","family":"Adelino","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Virologia e Riquetsioses, Instituto Oct\u00e1vio Magalh\u00e3es, Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte 30510-010, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Poxv\u00edrus, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3629-8529","authenticated-orcid":false,"given":"Felipe Campos de Melo","family":"Iani","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Virologia e Riquetsioses, Instituto Oct\u00e1vio Magalh\u00e3es, Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte 30510-010, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Poxv\u00edrus, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0830-3909","authenticated-orcid":false,"given":"Maur\u00edcio Teixeira","family":"Lima","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Virologia e Riquetsioses, Instituto Oct\u00e1vio Magalh\u00e3es, Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte 30510-010, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Poxv\u00edrus, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thalita Souza","family":"Arantes","sequence":"additional","affiliation":[{"name":"Centro de Microscopia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9841-9533","authenticated-orcid":false,"given":"Denilson Eduardo Silva","family":"Cunha","sequence":"additional","affiliation":[{"name":"Centro de Microscopia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7148-4012","authenticated-orcid":false,"given":"Rodrigo Ara\u00fajo Lima","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0785-4298","authenticated-orcid":false,"given":"Giliane de Souza","family":"Trindade","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Poxv\u00edrus, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2721-3826","authenticated-orcid":false,"given":"Erna Geessien","family":"Kroon","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Poxv\u00edrus, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nidia Esther Colquehuanca","family":"Arias","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gl\u00f3ria Regina","family":"Franco","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Bioqu\u00edmica, Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f4natas Santos","family":"Abrah\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Poxv\u00edrus, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/j.1699-0463.1959.tb00328.x","article-title":"A pox-like disease in 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