{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:44:54Z","timestamp":1788050694853,"version":"build-2784847793"},"reference-count":111,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LTGC24H050001"],"award-info":[{"award-number":["LTGC24H050001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Congenital cytomegalovirus infection is an underrecognized congenital infection. Globally, it impacts approximately 1 of every 200 live births. Although infected infants can have an increased risk of long-term sequelae, such as neurodevelopmental impairments and sensorineural hearing loss, most of the infected infants do not show visible signs at birth. As congenital cytomegalovirus infection often goes undetected and screening programs are not widely accepted, awareness of congenital cytomegalovirus in neonates is lacking. The aim of this study is to offer the current status of the epidemiology, clinical manifestations, and laboratory testing for the diagnosis of congenital cytomegalovirus infection and newborn screening approaches.<\/jats:p>","DOI":"10.3390\/v18010063","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T16:34:23Z","timestamp":1767198863000},"page":"63","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Screening for Congenital Cytomegalovirus Infection in Newborns"],"prefix":"10.3390","volume":"18","author":[{"given":"Junfeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The Children\u2019s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children\u2019s Regional Medical Center, Hangzhou 310052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Children\u2019s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children\u2019s Regional Medical Center, Hangzhou 310052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Children\u2019s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children\u2019s Regional Medical Center, Hangzhou 310052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/rmv.535","article-title":"Review and meta-analysis of the epidemiology of congenital cytomegalovirus (CMV) infection","volume":"17","author":"Kenneson","year":"2007","journal-title":"Rev. Med. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/CMR.00062-12","article-title":"The \u201csilent\u201d global burden of congenital cytomegalovirus","volume":"26","author":"Manicklal","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S178","DOI":"10.1093\/cid\/cit629","article-title":"Congenital cytomegalovirus infection: Clinical outcome","volume":"57","author":"Boppana","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1053\/j.spid.2004.09.011","article-title":"Congenital cytomegalovirus infection: Outcome and diagnosis","volume":"16","author":"Ross","year":"2005","journal-title":"Semin. Pediatr. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Cannon, M.J., and Davis, K.F. (2005). Washing our hands of the congenital cytomegalovirus disease epidemic. BMC Public Health, 5.","DOI":"10.1186\/1471-2458-5-70"},{"key":"ref_6","first-page":"35","article-title":"Surveillance of congenital cytomegalovirus disease, 1990\u20131991. Collaborating Registry Group","volume":"41","author":"Dobbins","year":"1992","journal-title":"MMWR CDC Surveill. Summ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1136\/archdischild-2018-316010","article-title":"Economic cost of congenital CMV in the UK","volume":"104","author":"Retzler","year":"2019","journal-title":"Arch. Dis. Child."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/rmv.679","article-title":"Implementing neonatal screening for congenital cytomegalovirus: Addressing the deafness of policy makers","volume":"21","author":"Vossen","year":"2011","journal-title":"Rev. Med. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1016\/j.jcv.2009.09.002","article-title":"Congenital cytomegalovirus (CMV) epidemiology and awareness","volume":"46","author":"Cannon","year":"2009","journal-title":"J. Clin. Virol."},{"key":"ref_10","first-page":"n1212","article-title":"Congenital cytomegalovirus infection","volume":"373","author":"Pesch","year":"2021","journal-title":"BMJ Clin. Res. Ed."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1097\/00006454-199202000-00007","article-title":"Symptomatic congenital cytomegalovirus infection: Neonatal morbidity and mortality","volume":"11","author":"Boppana","year":"1992","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1093\/cid\/cit018","article-title":"Long-term outcomes of congenital cytomegalovirus infection in Sweden and the United Kingdom","volume":"56","author":"Townsend","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1097\/INF.0000000000001763","article-title":"Congenital Cytomegalovirus: A European Expert Consensus Statement on Diagnosis and Management","volume":"36","author":"Luck","year":"2017","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e177","DOI":"10.1016\/S1473-3099(17)30143-3","article-title":"Congenital cytomegalovirus infection in pregnancy and the neonate: Consensus recommendations for prevention, diagnosis, and therapy","volume":"17","author":"Rawlinson","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e2034","DOI":"10.1002\/rmv.2034","article-title":"Estimation of the worldwide seroprevalence of cytomegalovirus: A systematic review and meta-analysis","volume":"29","author":"Zuhair","year":"2019","journal-title":"Rev. Med. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1017\/S0950268817000103","article-title":"Seroprevalence of cytomegalovirus infection in France in 2010","volume":"145","author":"Antona","year":"2017","journal-title":"Epidemiol. Infect."},{"key":"ref_17","first-page":"1","article-title":"Cytomegalovirus infection in pregnancy: Review of the literature","volume":"5","author":"Bonalumi","year":"2011","journal-title":"J. Prenat. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e2120736","DOI":"10.1001\/jamanetworkopen.2021.20736","article-title":"Congenital Cytomegalovirus Infection Burden and Epidemiologic Risk Factors in Countries with Universal Screening: A Systematic Review and Meta-analysis","volume":"4","author":"Ssentongo","year":"2021","journal-title":"JAMA Netw. Open"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.ajog.2020.02.018","article-title":"Cytomegalovirus infection during pregnancy: State of the science","volume":"223","author":"Foulon","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.ajog.2020.05.038","article-title":"Timing of primary maternal cytomegalovirus infection and rates of vertical transmission and fetal consequences","volume":"223","author":"Chatzakis","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s13052-017-0358-8","article-title":"Congenital Cytomegalovirus infection: Advances and challenges in diagnosis, prevention and treatment","volume":"43","author":"Marsico","year":"2017","journal-title":"Ital. J. Pediatr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.ogc.2023.02.002","article-title":"Cytomegalovirus in Pregnancy","volume":"50","author":"Canfield","year":"2023","journal-title":"Obstet. Gynecol. Clin. N. Am."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e606","DOI":"10.1542\/neo.22-9-e606","article-title":"Congenital Cytomegalovirus Infection: Epidemiology, Timely Diagnosis, and Management","volume":"22","author":"Jenks","year":"2021","journal-title":"NeoReviews"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/jmwh.13228","article-title":"Cytomegalovirus Infection in Pregnancy: Prevention, Presentation, Management and Neonatal Outcomes","volume":"66","author":"Pesch","year":"2021","journal-title":"J. Midwifery Women\u2019s Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1097\/QCO.0000000000000874","article-title":"Congenital cytomegalovirus infection diagnostics and management","volume":"35","author":"Pinninti","year":"2022","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1038\/jp.2017.41","article-title":"Long-term outcomes of children with symptomatic congenital cytomegalovirus disease","volume":"37","author":"Lanzieri","year":"2017","journal-title":"J. Perinatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e1938","DOI":"10.1002\/rmv.1938","article-title":"Hearing and neurodevelopmental outcomes for children with asymptomatic congenital cytomegalovirus infection: A systematic review","volume":"27","author":"Bartlett","year":"2017","journal-title":"Rev. Med. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1053\/j.semperi.2018.02.002","article-title":"Congenital cytomegalovirus infection","volume":"42","author":"Fowler","year":"2018","journal-title":"Semin. Perinatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104518","DOI":"10.1016\/j.jcv.2020.104518","article-title":"Maternal type of CMV infection and sequelae in infants with congenital CMV: Systematic review and meta-analysis","volume":"129","author":"Maltezou","year":"2020","journal-title":"J. Clin. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1055\/s-0040-1721557","article-title":"Neurological Manifestations of Congenital Cytomegalovirus Infection at a Tertiary Care Centre from Southern India","volume":"12","author":"Gowda","year":"2021","journal-title":"J. Neurosci. Rural Pract."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.jpeds.2013.12.007","article-title":"Spectrum of disease and outcome in children with symptomatic congenital cytomegalovirus infection","volume":"164","author":"Dreher","year":"2014","journal-title":"J. Pediatr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"100892","DOI":"10.1016\/j.lanepe.2024.100892","article-title":"Consensus recommendation for prenatal, neonatal and postnatal management of congenital cytomegalovirus infection from the European congenital infection initiative (ECCI)","volume":"40","author":"Chatzakis","year":"2024","journal-title":"Lancet Reg. Health Eur."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1542\/pir.23.5.163","article-title":"Cytomegalovirus infection","volume":"23","author":"Pass","year":"2002","journal-title":"Pediatr. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"274","DOI":"10.3928\/01913913-20120710-03","article-title":"Ophthalmological findings in congenital cytomegalovirus infection: When to screen, when to treat?","volume":"49","author":"Ghekiere","year":"2012","journal-title":"J. Pediatr. Ophthalmol. Strabismus"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/rmv.544","article-title":"New estimates of the prevalence of neurological and sensory sequelae and mortality associated with congenital cytomegalovirus infection","volume":"17","author":"Dollard","year":"2007","journal-title":"Rev. Med. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Salom\u00e8, S., Giannattasio, A., Malesci, R., Marciano, E., Dolce, P., Portella, G., Continisio, G.I., Di Costanzo, P., Capone, E., and Coppola, C. (2020). The Natural History of Hearing Disorders in Asymptomatic Congenital Cytomegalovirus Infection. Front. Pediatr., 8.","DOI":"10.3389\/fped.2020.00217"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1055\/s-0042-1748054","article-title":"Longitudinal investigation of hearing disorders in children with congenital cytomegalovirus","volume":"11","author":"Dahle","year":"2000","journal-title":"J. Am. Acad. Audiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1542\/peds.2014-1173","article-title":"Hearing loss and congenital CMV infection: A systematic review","volume":"134","author":"Goderis","year":"2014","journal-title":"Pediatrics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1093\/oxfordjournals.aje.a121151","article-title":"Primary infection with human cytomegalovirus: Virus isolation from healthy infants and pregnant women","volume":"91","author":"Numazaki","year":"1970","journal-title":"Am. J. Epidemiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1128\/CMR.15.4.680-715.2002","article-title":"Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant","volume":"15","author":"Revello","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1128\/jcm.19.6.917-919.1984","article-title":"Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen","volume":"19","author":"Gleaves","year":"1984","journal-title":"J. Clin. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1093\/infdis\/152.3.631","article-title":"Rapid detection of human cytomegalovirus in the urine of humans","volume":"152","author":"Alpert","year":"1985","journal-title":"J. Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0166-0934(90)90137-5","article-title":"Early detection of cytomegalovirus in cell culture by a new monoclonal antibody, CCH2","volume":"27","author":"Landqvist","year":"1990","journal-title":"J. Virol. Methods"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1093\/infdis\/167.6.1433","article-title":"Neonatal screening for congenital cytomegalovirus infection by detection of virus in saliva","volume":"167","author":"Balcarek","year":"1993","journal-title":"J. Infect. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1086\/341831","article-title":"Diagnosis and outcome of preconceptional and periconceptional primary human cytomegalovirus infections","volume":"186","author":"Revello","year":"2002","journal-title":"J. Infect. Dis."},{"key":"ref_46","first-page":"466","article-title":"Intraperitoneal administration of cytomegalovirus hyperimmunoglobulin to the cytomegalovirus-infected fetus","volume":"18","author":"Negishi","year":"1998","journal-title":"J. Perinatol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1515\/CCLM.2001.014","article-title":"Evaluation of five commercial enzyme immunoassays for the detection of human cytomegalovirus-specific IgM antibodies in the absence of a commercially available gold standard","volume":"39","author":"Genser","year":"2001","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/CDLI.8.1.196-198.2001","article-title":"Evaluation of the Abbott AxSYM cytomegalovirus (CMV) immunoglobulin M (IgM) assay in conjunction with other CMV IgM tests and a CMV IgG avidity assay","volume":"8","author":"Lazzarotto","year":"2001","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1007\/s10096-009-0738-0","article-title":"Measurement of the sensitivity of different commercial assays in the diagnosis of CMV infection in pregnancy","volume":"28","author":"Gentile","year":"2009","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.jcv.2010.07.024","article-title":"Laboratory evaluation of the new Access\u00ae cytomegalovirus immunoglobulin IgM and IgG assays","volume":"49","author":"Carlier","year":"2010","journal-title":"J. Clin. Virol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1128\/CVI.00332-10","article-title":"Multiplex detection of IgM and IgG class antibodies to Toxoplasma gondii, rubella virus, and cytomegalovirus using a novel multiplex flow immunoassay","volume":"17","author":"Binnicker","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1097\/PAT.0b013e328353bec0","article-title":"Comparison of five CMV IgM immunoassays with CMV IgG avidity for diagnosis of primary CMV infection","volume":"44","author":"BaAlawi","year":"2012","journal-title":"Pathology"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Kitamura, A., Toriyabe, K., Hagimoto-Akasaka, M., Hamasaki-Shimada, K., Ikejiri, M., Minematsu, T., Suga, S., Kondo, E., Kihira, M., and Morikawa, F. (2023). Revision of Cytomegalovirus Immunoglobulin M Antibody Titer Cutoff in a Maternal Antibody Screening Program in Japan: A Cohort Comparison Involving a Total of 32,000 Pregnant Women. Viruses, 15.","DOI":"10.3390\/v15040962"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Gerna, G., Fornara, C., Furione, M., and Lilleri, D. (2021). Congenital Human Cytomegalovirus Infection: A Narrative Review of Maternal Immune Response and Diagnosis in View of the Development of a Vaccine and Prevention of Primary and Non-Primary Infections in Pregnancy. Microorganisms, 9.","DOI":"10.3390\/microorganisms9081749"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e42","DOI":"10.1111\/1471-0528.17966","article-title":"Congenital Cytomegalovirus Infection: Update on Screening, Diagnosis and Treatment: Scientific Impact Paper No. 56","volume":"132","author":"Khalil","year":"2025","journal-title":"BJOG Int. J. Obstet. Gynaecol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e29525","DOI":"10.1002\/jmv.29525","article-title":"Estimated cytomegalovirus seroprevalence in the general population of the United States and Canada","volume":"96","author":"Lally","year":"2024","journal-title":"J. Med. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e0031323","DOI":"10.1128\/jcm.00313-23","article-title":"Maternal and congenital human cytomegalovirus infection: Laboratory testing for detection and diagnosis","volume":"62","author":"Leber","year":"2024","journal-title":"J. Clin. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1128\/CVI.00487-14","article-title":"Role of cytomegalovirus (CMV) IgG avidity testing in diagnosing primary CMV infection during pregnancy","volume":"21","author":"Prince","year":"2014","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.jcv.2012.09.015","article-title":"The value of CMV IgG avidity and immunoblot for timing the onset of primary CMV infection in pregnancy","volume":"56","author":"Enders","year":"2013","journal-title":"J. Clin. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jcv.2012.10.017","article-title":"Re-evaluation of the VIDAS\u00ae cytomegalovirus (CMV) IgG avidity assay: Determination of new cut-off values based on the study of kinetics of CMV-IgG maturation","volume":"56","author":"Berth","year":"2013","journal-title":"J. Clin. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Abdullahi Nasir, I., Babayo, A., and Shehu, M.S. (2016). Clinical Significance of IgG Avidity Testing and Other Considerations in the Diagnosis of Congenital Cytomegalovirus Infection: A Review Update. Med. Sci., 4.","DOI":"10.3390\/medsci4010005"},{"key":"ref_62","first-page":"297","article-title":"The role of IgG avidity in diagnosis of cytomegalovirus infection in newborns and infants","volume":"36","author":"Sviben","year":"2012","journal-title":"Coll. Antropol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1128\/JCM.40.3.746-752.2002","article-title":"Role of the laboratory in diagnosis and management of cytomegalovirus infection in hematopoietic stem cell and solid-organ transplant recipients","volume":"40","author":"Razonable","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1386-6532(99)00016-5","article-title":"Diagnostic and prognostic value of human cytomegalovirus load and IgM antibody in blood of congenitally infected newborns","volume":"14","author":"Revello","year":"1999","journal-title":"J. Clin. Virol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1128\/microbiolspec.DMIH2-0022-2015","article-title":"Cytomegalovirus","volume":"4","author":"Dioverti","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"131","DOI":"10.12659\/AOT.895677","article-title":"Cytomegalovirus (CMV) Monitoring After Liver Transplantation: Comparison of CMV Pp65 Antigenemia Assay with Real-Time PCR Calibrated to WHO International Standard","volume":"21","author":"Kamei","year":"2016","journal-title":"Ann. Transplant."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.jcv.2011.11.006","article-title":"Real-time PCR versus viral culture on urine as a gold standard in the diagnosis of congenital cytomegalovirus infection","volume":"53","author":"Wolthers","year":"2012","journal-title":"J. Clin. Virol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1128\/JCM.02502-06","article-title":"Real-time PCR assay using specimens on filter disks as a template for detection of cytomegalovirus in urine","volume":"45","author":"Nozawa","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1056\/NEJMoa1006561","article-title":"Saliva polymerase-chain-reaction assay for cytomegalovirus screening in newborns","volume":"364","author":"Boppana","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1097\/INF.0000000000000609","article-title":"Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection","volume":"34","author":"Pinninti","year":"2015","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1093\/infdis\/jiu263","article-title":"Detection of congenital cytomegalovirus infection by real-time polymerase chain reaction analysis of saliva or urine specimens","volume":"210","author":"Ross","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e0178122","DOI":"10.1128\/jcm.01781-22","article-title":"Rapid and Direct Detection of Congenital Cytomegalovirus Using a Commercial Real-Time PCR Assay","volume":"61","author":"Fernholz","year":"2023","journal-title":"J. Clin. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1097\/INF.0000000000000757","article-title":"Urine Collection Method for the Diagnosis of Congenital Cytomegalovirus Infection","volume":"34","author":"Ross","year":"2015","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e000118","DOI":"10.1136\/bmjopen-2011-000118","article-title":"Screening for congenital cytomegalovirus infection using newborn urine samples collected on filter paper: Feasibility and outcomes from a multicentre study","volume":"1","author":"Koyano","year":"2011","journal-title":"BMJ Open"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1093\/infdis\/jiz373","article-title":"Saliva Real-Time Polymerase Chain Reaction for Targeted Screening of Congenital Cytomegalovirus Infection","volume":"220","author":"Manor","year":"2019","journal-title":"J. Infect. Dis."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e205441","DOI":"10.1001\/jamapediatrics.2020.5441","article-title":"Sensitivity of Dried Blood Spot Testing for Detection of Congenital Cytomegalovirus Infection","volume":"175","author":"Dollard","year":"2021","journal-title":"JAMA Pediatr."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Nagel, A., Dimitrakopoulou, E., Teig, N., Kern, P., L\u00fccke, T., Michna, D., Korn, K., Steininger, P., Shahada, K., and Neumann, K. (2020). Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0227143"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1093\/infdis\/jiy057","article-title":"Contribution of Breastfeeding to False-Positive Saliva Polymerase Chain Reaction for Newborn Congenital Cytomegalovirus Screening","volume":"217","author":"Ross","year":"2018","journal-title":"J. Infect. Dis."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"S74","DOI":"10.1093\/infdis\/jiz601","article-title":"Clinical Diagnostic Testing for Human Cytomegalovirus Infections","volume":"221","author":"Razonable","year":"2020","journal-title":"J. Infect. Dis."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jcv.2019.05.015","article-title":"Primary cytomegalovirus (CMV) infection in pregnancy: Diagnostic value of CMV PCR in saliva compared to urine at birth","volume":"117","author":"Exler","year":"2019","journal-title":"J. Clin. Virol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jcv.2006.03.011","article-title":"Is saliva as reliable as urine for detection of cytomegalovirus DNA for neonatal screening of congenital CMV infection?","volume":"36","author":"Yamamoto","year":"2006","journal-title":"J. Clin. Virol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jcv.2014.06.002","article-title":"Urine is superior to saliva when screening for postnatal CMV infections in preterm infants","volume":"61","author":"Gunkel","year":"2014","journal-title":"J. Clin. Virol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s00431-025-06015-w","article-title":"Can viral load predict a symptomatic congenital CMV infection? A systematic review and meta-analysis","volume":"184","author":"Gammella","year":"2025","journal-title":"Eur. J. Pediatr."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"F280","DOI":"10.1136\/adc.2007.119230","article-title":"Congenital cytomegalovirus: Association between dried blood spot viral load and hearing loss","volume":"93","author":"Walter","year":"2008","journal-title":"Arch. Dis. Child. Fetal Neonatal Ed."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1086\/426456","article-title":"Detection of cytomegalovirus (CMV) DNA by polymerase chain reaction is associated with hearing loss in newborns with symptomatic congenital CMV infection involving the central nervous system","volume":"191","author":"Bradford","year":"2005","journal-title":"J. Infect. Dis."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/rmv.695","article-title":"Review of cytomegalovirus shedding in bodily fluids and relevance to congenital cytomegalovirus infection","volume":"21","author":"Cannon","year":"2011","journal-title":"Rev. Med. Virol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"4559","DOI":"10.1002\/jmv.27844","article-title":"Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR","volume":"94","author":"Yamaguchi","year":"2022","journal-title":"J. Med. Virol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/infdis\/jiu627","article-title":"High Cytomegalovirus (CMV) DNAemia Predicts CMV Sequelae in Asymptomatic Congenitally Infected Newborns Born to Women with Primary Infection During Pregnancy","volume":"212","author":"Forner","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1093\/jpids\/piad013","article-title":"Urine and Saliva Viral Load in Children with Congenital Cytomegalovirus Infection","volume":"12","author":"Kabani","year":"2023","journal-title":"J. Pediatr. Infect. Dis. Soc."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1097\/INF.0000000000003205","article-title":"The Limitations of Cytomegalovirus DNA Detection in Cerebrospinal Fluid of Newborn Infants with Congenital CMV Infection: A Tertiary Care Neonatal Center Experience","volume":"40","author":"Kasztelewicz","year":"2021","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0928-0197(96)00228-0","article-title":"Diagnosis of congenital cytomegalovirus infection by detection of viral DNA in dried blood spots","volume":"6","author":"Barbi","year":"1996","journal-title":"Clin. Diagn. Virol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1002\/jmv.21543","article-title":"Use of stored dried blood spots for retrospective diagnosis of congenital CMV","volume":"81","author":"Atkinson","year":"2009","journal-title":"J. Med. Virol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.jpeds.2010.03.002","article-title":"Use of screening dried blood spots for estimation of prevalence, risk factors, and birth outcomes of congenital cytomegalovirus infection","volume":"157","author":"Kharrazi","year":"2010","journal-title":"J. Pediatr."},{"key":"ref_94","first-page":"1503","article-title":"Retrospective diagnosis of congenital CMV infection in DBS from Guthrie cards: French experience","volume":"16","author":"Couderc","year":"2009","journal-title":"Arch. Pediatr."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S1386-6532(00)00089-5","article-title":"Cytomegalovirus DNA detection in Guthrie cards: A powerful tool for diagnosing congenital infection","volume":"17","author":"Barbi","year":"2000","journal-title":"J. Clin. Virol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1001\/jama.2010.423","article-title":"Dried blood spot real-time polymerase chain reaction assays to screen newborns for congenital cytomegalovirus infection","volume":"303","author":"Boppana","year":"2010","journal-title":"JAMA"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jcv.2015.03.015","article-title":"Evaluation of DNA extraction methods for the detection of Cytomegalovirus in dried blood spots","volume":"66","author":"Koontz","year":"2015","journal-title":"J. Clin. Virol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"e0404122","DOI":"10.1128\/spectrum.04041-22","article-title":"Improved Dried Blood Spot PCR Assay for Universal Congenital Cytomegalovirus Screening in Newborns","volume":"11","author":"Kim","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1128\/JCM.01868-16","article-title":"Testing for Cytomegalovirus in Pregnancy","volume":"55","author":"Saldan","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/uog.26039","article-title":"New data on efficacy of valacyclovir in secondary prevention of maternal-fetal transmission of cytomegalovirus","volume":"61","author":"Egloff","year":"2023","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/1471-0528.16966","article-title":"Leaning towards Cytomegalovirus serological screening in pregnancy to prevent congenital infection: A cost-effectiveness perspective","volume":"129","author":"Seror","year":"2022","journal-title":"BJOG Int. J. Obstet. Gynaecol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1542\/peds.2006-3441","article-title":"Newborn hearing screening and detection of congenital cytomegalovirus infection","volume":"121","author":"Stehel","year":"2008","journal-title":"Pediatrics"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1097\/00006454-200301000-00012","article-title":"A wider role for congenital cytomegalovirus infection in sensorineural hearing loss","volume":"22","author":"Barbi","year":"2003","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"e20162128","DOI":"10.1542\/peds.2016-2128","article-title":"A Targeted Approach for Congenital Cytomegalovirus Screening Within Newborn Hearing Screening","volume":"139","author":"Fowler","year":"2017","journal-title":"Pediatrics"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1136\/archdischild-2022-323809","article-title":"Managing challenges in congenital CMV: Current thinking","volume":"108","author":"Jones","year":"2023","journal-title":"Arch. Dis. Child."},{"key":"ref_106","first-page":"3","article-title":"Newborn Screening for Congenital Cytomegalovirus (cCMV) Infection: Universal, Targeted, Expanded-Targeted, or None-of-the-Above?","volume":"19","author":"Schleiss","year":"2024","journal-title":"Neonatol. Today"},{"key":"ref_107","first-page":"100257","article-title":"Human cytomegalovirus and neonatal infection","volume":"7","author":"Lawrence","year":"2024","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/cga.12512","article-title":"Expanded targeted screening for congenital cytomegalovirus infection","volume":"63","author":"Zhang","year":"2023","journal-title":"Congenit. Anom."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1001\/jamapediatrics.2016.2016","article-title":"Cost-effectiveness of Universal and Targeted Newborn Screening for Congenital Cytomegalovirus Infection","volume":"170","author":"Gantt","year":"2016","journal-title":"JAMA Pediatr."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"ofae311","DOI":"10.1093\/ofid\/ofae311","article-title":"A Geographically Weighted Cost-effectiveness Analysis of Newborn Cytomegalovirus Screening","volume":"11","author":"Lantos","year":"2024","journal-title":"Open Forum Infect. Dis."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1002\/rmv.1790","article-title":"Universal newborn screening for congenital CMV infection: What is the evidence of potential benefit?","volume":"24","author":"Cannon","year":"2014","journal-title":"Rev. Med. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/1\/63\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T05:13:31Z","timestamp":1767590011000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/1\/63"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,31]]},"references-count":111,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["v18010063"],"URL":"https:\/\/doi.org\/10.3390\/v18010063","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,31]]}}}