{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:29:04Z","timestamp":1767677344119,"version":"3.48.0"},"reference-count":34,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PICT 2020 \u2014 Serie A N\u00ba 01012"],"award-info":[{"award-number":["PICT 2020 \u2014 Serie A N\u00ba 01012"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Bovine gammaherpesvirus 4 (BoGHV-4) is an opportunistic uterine pathogen whose reactivation is associated with postpartum inflammation and bacterial lipopolysaccharide (LPS). Platelet-rich plasma (PRP) is a regenerative biotherapeutic capable of modulating inflammatory responses, although its effects may vary depending on BoGHV4 genotype. In this study, primary bovine endometrial cells (BECs) were cultured in medium containing 10% PRP instead of fetal bovine serum, infected with two genetically divergent BoGHV-4 isolates (07-435, genotype 3; 10-154, genotype 2), and subsequently stimulated with bacterial lipopolysaccharide (LPS, 100 ng\/mL). Expression of the viral immediate-early gene IE-2 and host immune genes (TLR4, TNF-\u03b1, CXCL8, and IFN-\u03b3) were quantified by RT-qPCR from 4 to 48 h after stimulation. Isolate 07-435 induced a sustained activation of IE-2 and gradual cytokine upregulation, while isolate 10-154 elicited an early but transient inflammatory response followed by gene downregulation. PRP did not modify the strain-specific patterns of viral and inflammatory gene expression but established a common inflammatory baseline, whereas the magnitude and temporal profile of the response continued to be dictated by the viral genotype. These findings indicate that BoGHV-4 genotypic diversity remained the main determinant of response intensity and duration, supporting PRP as a context-dependent rather than a universal antiviral modulator.<\/jats:p>","DOI":"10.3390\/v18010064","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T16:34:23Z","timestamp":1767198863000},"page":"64","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["BoGHV-4 Genotypic Diversity Shapes Inflammatory and Viral Gene Expression in Platelet-Rich Plasma-Supplemented Bovine Endometrial Cells"],"prefix":"10.3390","volume":"18","author":[{"given":"Sofia","family":"L\u00f3pez","sequence":"first","affiliation":[{"name":"Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible (IPADS) (INTA\u2013CONICET), Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"},{"name":"Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignacio","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Laboratorio \u00c1lvarez, 25 de Mayo 139, Bah\u00eda Blanca B8000, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Delgado","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"},{"name":"Escola Superior de Agricultura Luiz de Queiroz, Universidad de S\u00e3o Pablo, Piracicaba CEP 13418-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6796-4408","authenticated-orcid":false,"given":"Valentina","family":"Andreoli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Medico Veterinarie, Universit\u00e0 di Parma, Via del Taglio 8, 43126 Parma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naiara","family":"Urrutia Luna","sequence":"additional","affiliation":[{"name":"Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible (IPADS) (INTA\u2013CONICET), Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"},{"name":"Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3506-1739","authenticated-orcid":false,"given":"Marisol","family":"Yavorsky","sequence":"additional","affiliation":[{"name":"Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible (IPADS) (INTA\u2013CONICET), Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"},{"name":"Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Pereyra","sequence":"additional","affiliation":[{"name":"Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible (IPADS) (INTA\u2013CONICET), Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4380-5725","authenticated-orcid":false,"given":"Stefano","family":"Grolli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Medico Veterinarie, Universit\u00e0 di Parma, Via del Taglio 8, 43126 Parma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7193-0262","authenticated-orcid":false,"given":"Erika","family":"Gonz\u00e1lez Altamiranda","sequence":"additional","affiliation":[{"name":"Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible (IPADS) (INTA\u2013CONICET), Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (CIVETAN), Tandil B7000GHG, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3317-7331","authenticated-orcid":false,"given":"Andrea","family":"Verna","sequence":"additional","affiliation":[{"name":"Instituto de Innovaci\u00f3n para la Producci\u00f3n Agropecuaria y el Desarrollo Sostenible (IPADS) (INTA\u2013CONICET), Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1530\/REP-08-0171","article-title":"Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation: A new insight into bacterial and viral interaction for uterine disease","volume":"136","author":"Donofrio","year":"2008","journal-title":"Reproduction"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1095\/biolreprod.112.106740","article-title":"Bovine endometrial stromal cells support tumor necrosis factor alpha-induced bovine herpesvirus type 4 enhanced replication","volume":"88","author":"Jacca","year":"2013","journal-title":"Biol. 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