{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T09:40:02Z","timestamp":1768038002311,"version":"3.49.0"},"reference-count":60,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011880","name":"ARES","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011880","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Cassava (Manihot esculenta) is a staple crop in sub-Saharan Africa threatened by several viral diseases. Here, we describe the genome sequence of a novel bipartite cheravirus (family Secoviridae) infecting cassava in the Democratic Republic of Congo and Tanzania. We designate the new virus \u201ccassava Congo cheravirus\u201d. Each RNA segment encodes a single polyprotein (P1 and P2 for RNA1 and RNA2, respectively), embedded with various putative cleavage sites (six and three in P1 and P2, respectively), consistent with members of the genus Cheravirus. We note two new features in the P1: (i) the presence of two domains, X1 and X2, upstream of the putative helicase region, which we also predict in other cheraviruses and (ii) the presence of a Maf\/HAM1-like inosine triphosphatase (ITPase) domain, a rare motif among viruses only previously detected in three potyviruses and a torradovirus, all of which infect plants from the Euphorbia family. Phylogenetic analyses placed the virus firmly within the genus Cheravirus, with amino acid identities in the Pro-Pol and coat protein regions well below existing ICTV species thresholds, supporting its classification as a virus belonging to a new species in the Cheravirus genus. Spatially distinct isolates from Bas-Congo, South-Kivu, and Tanzania form three genetic clusters, with evidence of recombination in both RNA segments. These results expand the known diversity of cassava viruses and suggest possible adaptation to the cassava host via ITPase acquisition.<\/jats:p>","DOI":"10.3390\/v18010084","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T09:01:29Z","timestamp":1767862889000},"page":"84","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Organization of the Newly Discovered Cassava Congo Cheravirus Reveals a Unique Maf\/HAM1 Motif in the C-Terminal Region of the RNA1 Polyprotein and Suggests the Presence of Two Protein Domains Upstream of the Putative Helicase Domain"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8578-0584","authenticated-orcid":false,"given":"Yves Bisimwa","family":"Kwibuka","sequence":"first","affiliation":[{"name":"Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Li\u00e8ge, 5030 Gembloux, Belgium"},{"name":"Department of Crop Production, Agriculture Sciences and Environment Faculty, Catholic University of Bukavu, Bukavu, Democratic Republic of the Congo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0309-9934","authenticated-orcid":false,"given":"Stephan","family":"Winter","sequence":"additional","affiliation":[{"name":"Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Espoir Basengere","family":"Bisimwa","sequence":"additional","affiliation":[{"name":"Department of Crop Production, Agriculture Sciences and Environment Faculty, Catholic University of Bukavu, Bukavu, Democratic Republic of the Congo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2444-2386","authenticated-orcid":false,"given":"Kumar","family":"Vasudevan","sequence":"additional","affiliation":[{"name":"Plant Genetics and Rhizospheric Processes Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Li\u00e8ge, 5030 Gembloux, Belgium"},{"name":"Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, Biosystems Department, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3807-3590","authenticated-orcid":false,"given":"H\u00e9l\u00e8ne","family":"Sanfa\u00e7on","sequence":"additional","affiliation":[{"name":"Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland V0H 1Z0, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"Vanderschuren","sequence":"additional","affiliation":[{"name":"Plant Genetics and Rhizospheric Processes Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Li\u00e8ge, 5030 Gembloux, Belgium"},{"name":"Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, Biosystems Department, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9bastien","family":"Massart","sequence":"additional","affiliation":[{"name":"Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Li\u00e8ge, 5030 Gembloux, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1007\/s12571-017-0717-8","article-title":"Crops that feed the world: Production and improvement of cassava for food, feed, and industrial uses","volume":"9","author":"Parmar","year":"2017","journal-title":"Food Secur."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kabirou Dodo, M. (2021). Understanding Africa\u2019s Food Security Challenges. Food Security in Africa, IntechOpen.","DOI":"10.5772\/intechopen.91773"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s12042-012-9096-7","article-title":"Is Cassava the Answer to African Climate Change Adaptation?","volume":"5","author":"Jarvis","year":"2012","journal-title":"Trop. Plant Biol."},{"key":"ref_4","unstructured":"Lebot, V. (2022, October 15). Tropical Root and Tuber Crops: Cassava, Sweet Potato, Yams and Aroids; Crop Production Science in Horticulture, 17; CABI: Wallingford, Oxfordshire, UK, 2009. Available online: https:\/\/books.google.be\/books?id=rFwyrKRSMUMC."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3572","DOI":"10.3390\/su2113572","article-title":"Cassava: The Drought, War and Famine Crop in a Changing World","volume":"2","author":"Burns","year":"2010","journal-title":"Sustainability"},{"key":"ref_6","unstructured":"Howeler, R.H. (2012). The Cassava Handbook: A Reference Manual based on the Asian Regional Cassava Training Course Held in Thailand, The International Center for Tropical Agriculture (CIAT)."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1080\/07388551.2022.2048791","article-title":"The future of cassava in the era of biotechnology in Southern Africa","volume":"43","author":"Otun","year":"2022","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kwibuka, Y., Bisimwa, E., Blouin, A.G., Bragard, C., Candresse, T., Faure, C., Filloux, D., Lett, J.-M., Maclot, F., and Marais, A. (2021). Novel ampeloviruses infecting cassava in central Africa and the south-west Indian ocean islands. Viruses, 13.","DOI":"10.3390\/v13061030"},{"key":"ref_9","unstructured":"Maruthi, M.N. (2015). Limiting the impact of Cassava Brown Streak Disease on Smalholders, Womens and the Cassava Value Chain, Interim Project Narrative Report Submitted to the African Union Commission, African Union Commission."},{"key":"ref_10","first-page":"13","article-title":"African cassava mosaic virus disease: The magnitude of the problem","volume":"2","author":"Thresh","year":"1997","journal-title":"Afr. J. Root Tuber Crop"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0065-3527(06)67010-3","article-title":"Cassava mosaic virus disease in East and Central Africa: Epidemiology and management of a regional pandemic","volume":"67","author":"Legg","year":"2006","journal-title":"Adv. Virus Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1146\/annurev-virology-101416-041913","article-title":"Cassava Mosaic and Brown Streak Diseases: Current Perspectives and Beyond","volume":"4","author":"Rey","year":"2017","journal-title":"Annu. Rev. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.pbi.2017.04.008","article-title":"Tackling agriculturally relevant diseases in the staple crop cassava (Manihot esculenta)","volume":"38","author":"McCallum","year":"2017","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"29648","DOI":"10.1038\/s41598-024-81142-2","article-title":"Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas","volume":"14","author":"Jimenez","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1007\/s00705-021-05325-2","article-title":"Complete genome sequence of a novel secovirid infecting cassava in the Americas","volume":"167","author":"Leiva","year":"2022","journal-title":"Arch. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1007\/s00705-009-0367-z","article-title":"Secoviridae: A proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus","volume":"154","author":"Wellink","year":"2009","journal-title":"Arch. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1007\/s00705-020-04752-x","article-title":"Changes to virus taxonomy and the Statutes ratified by the International Committee on Taxonomy of Viruses (2020)","volume":"165","author":"Walker","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1099\/jgv.0.000779","article-title":"ICTV virus taxonomy profile: Secoviridae","volume":"98","author":"Thompson","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s00705-019-04468-7","article-title":"Proposed revision of the family Secoviridae taxonomy to create three subgenera, \u2018Satsumavirus\u2019, \u2018Stramovirus\u2019 and \u2018Cholivirus\u2019, in the genus Sadwavirus","volume":"165","author":"Dasgupta","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1099\/jgv.0.000911","article-title":"ICTV virus taxonomy profile: Picornaviridae","volume":"98","author":"Zell","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1155\/2019\/9732325","article-title":"The Roles of Environmental Factors in Regulation of Oxidative Stress in Plant","volume":"2019","author":"Xie","year":"2019","journal-title":"BioMed Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1111\/nph.17924","article-title":"A new biochemistry connecting pathogen detection to induced defense in plants","volume":"234","author":"Parker","year":"2022","journal-title":"New Phytol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zamzami, M.A. (2022). Inosine Triphosphate Pyrophosphatase (ITPase): Functions, Mutations, Polymorphisms and Its Impact on Cancer Therapies. Cells, 11.","DOI":"10.3390\/cells11030384"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6934","DOI":"10.1128\/JVI.00537-09","article-title":"Cassava brown streak virus (Potyviridae) encodes a putative Maf\/HAM1 pyrophosphatase implicated in reduction of mutations and a P1 proteinase that suppresses RNA silencing but contains no HC-Pro","volume":"83","author":"Mbanzibwa","year":"2009","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s00705-016-3087-1","article-title":"Analysis of the complete genome sequence of euphorbia ringspot virus, an atypical member of the genus Potyvirus","volume":"162","author":"Knierim","year":"2017","journal-title":"Arch. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/s00705-015-2679-5","article-title":"Complete genome sequence of currant latent virus (genus Cheravirus, family Secoviridae)","volume":"161","author":"Petrzik","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12985-023-02257-y","article-title":"Mixed infection of ITPase-encoding potyvirid and secovirid in Mercurialis perennis: Evidences for a convergent euphorbia-specific viral counterstrike","volume":"21","author":"Mahillon","year":"2024","journal-title":"Virol. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1111\/mpp.13021","article-title":"Viral inosine triphosphatase: A mysterious enzyme with typical activity, but an atypical function","volume":"22","author":"James","year":"2021","journal-title":"Mol. Plant Pathol."},{"key":"ref_29","unstructured":"Lopez, P.-G. (2022). Cassava and Ugandan cassava brown streak virus, an attractive case of plant-pathogen coevolution. III Reunion de la Red Nacional de Virologia de Plantas, RENAVIPLANT."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1111\/mpp.12813","article-title":"Cassava brown streak virus Ham1 protein hydrolyses mutagenic nucleotides and is a necrosis determinant","volume":"20","author":"Tomlinson","year":"2019","journal-title":"Mol. Plant Pathol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/0042-6822(66)90030-4","article-title":"Purification and properties of tomato ringspot virus and an RNA-deficient component","volume":"29","year":"1966","journal-title":"Virology"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kwibuka, Y., Nyirakanani, C., Bizimana, J.P., Bisimwa, E., Brostaux, Y., Lassois, L., Vanderschuren, H., and Massart, S. (2022). Risk factors associated with cassava brown streak disease dissemination through seed pathways in Eastern D.R. Congo. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.803980"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"D20","DOI":"10.1093\/nar\/gkab1112","article-title":"Database resources of the national center for biotechnology information","volume":"50","author":"Sayers","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"vev003","DOI":"10.1093\/ve\/vev003","article-title":"RDP4: Detection and analysis of recombination patterns in virus genomes","volume":"1","author":"Martin","year":"2015","journal-title":"Virus Evol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"W636","DOI":"10.1093\/nar\/gkz268","article-title":"The EMBL-EBI search and sequence analysis tools APIs in 2019","volume":"47","author":"Madeira","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2771","DOI":"10.1099\/0022-1317-81-11-2771","article-title":"Proteolytic processing at a novel cleavage site in the N-terminal region of the tomato ringspot nepovirus RNA-1-encoded polyprotein in vitro","volume":"81","author":"Wang","year":"2000","journal-title":"J. Gen. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.virol.2008.01.046","article-title":"Characterization of proteinase cleavage sites in the N-terminal region of the RNA1-encoded polyprotein from Arabis mosaic virus (subgroup A nepovirus)","volume":"375","author":"Wetzel","year":"2008","journal-title":"Virology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1128\/jvi.71.3.2436-2448.1997","article-title":"The refined crystal structure of the 3C gene product from hepatitis A virus: Specific proteinase activity and RNA recognition","volume":"71","author":"Bergmann","year":"1997","journal-title":"J. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1007\/s00705-022-05564-x","article-title":"Re-examination of nepovirus polyprotein cleavage sites highlights the diverse specificities and evolutionary relationships of nepovirus 3C-like proteases","volume":"167","year":"2022","journal-title":"Arch. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1093\/nar\/gkq1019","article-title":"The sequence read archive","volume":"39","author":"Leinonen","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Babaian, A., and Edgar, R.C. (2021). Ribovirus classification by a polymerase barcode sequence. bioRxiv.","DOI":"10.1101\/2021.03.02.433648"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/s41586-021-04332-2","article-title":"Petabase-scale sequence alignment catalyses viral discovery","volume":"602","author":"Edgar","year":"2022","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"W537","DOI":"10.1093\/nar\/gky379","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update","volume":"46","author":"Afgan","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Ndunguru, J., Sseruwagi, P., Tairo, F., Stomeo, F., Maina, S., Djinkeng, A., Kehoe, M., and Boykin, L.M. (2015). Analyses of twelve new whole genome sequences of cassava brown streak viruses and ugandan cassava brown streak viruses from East Africa: Diversity, supercomputing and evidence for further speciation. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0139321"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (iTOL) v5: An online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1099\/0022-1317-81-2-541","article-title":"Nucleotide sequence and genome organization of Apple latent spherical virus: A new virus classified into the family Comoviridae","volume":"81","author":"Li","year":"2000","journal-title":"Microbiology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1007\/s00705-002-0833-3","article-title":"Nucleotide sequence analysis of RNA-2 of a flat apple isolate of Cherry rasp leaf virus with regions showing greater identity to animal picornaviruses than to related plant viruses","volume":"147","author":"James","year":"2002","journal-title":"Arch. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1007\/s00705-012-1546-x","article-title":"Complete genome sequence of arracacha virus B: A novel cheravirus","volume":"158","author":"Adams","year":"2013","journal-title":"Arch. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1007\/s00705-005-0503-3","article-title":"Genome segment RNA-1 of a flat apple isolate of Cherry rasp leaf virus: Nucleotide sequence analysis and RT-PCR detection","volume":"150","author":"James","year":"2005","journal-title":"Arch. Virol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Mann, K.S., Walker, M., and Sanfa\u00e7on, H. (2017). Identification of cleavage sites recognized by the 3C-like cysteine protease within the two polyproteins of strawberry mottle virus. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00745"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"10847","DOI":"10.1128\/JVI.00789-06","article-title":"Characterization of Membrane Association Domains within the Tomato Ringspot Nepovirus X2 Protein, an Endoplasmic Reticulum-Targeted Polytopic Membrane Protein","volume":"80","author":"Zhang","year":"2006","journal-title":"J. Virol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-2836(82)90515-0","article-title":"A simple method for displaying the hydropathic character of a protein","volume":"157","author":"Kyte","year":"1982","journal-title":"J. Mol. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1016\/j.biochi.2013.05.016","article-title":"Analysis of human ITPase nucleobase specificity by site-directed mutagenesis","volume":"95","author":"Gall","year":"2013","journal-title":"Biochimie"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Valli, A.A., Gonzalo, I., Pasin, F., Montanuy, I., and Antonio, J. (2021). Maf\/ham1-like pyrophosphatases, host-specific partners of viral RNA-dependent RNA polymerases. bioRxiv.","DOI":"10.1101\/2021.05.18.444600"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Valli, A.A., Garc\u00eda L\u00f3pez, R., Ribaya, M., Mart\u00ednez, F.J., G\u00f3mez, D.G., Garc\u00eda, B., Gonzalo, I., Gonzalez de Pradena, A., Pasin, F., and Montanuy, I. (2022). Maf\/ham1-like pyrophosphatases of noncanonical nucleotides are host-specific partners of viral RNA-dependent RNA polymerases. PLoS Pathog., 18.","DOI":"10.1371\/journal.ppat.1010332"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.virol.2016.08.014","article-title":"Identification of the cleavage sites of the RNA2-encoded polyproteins for two members of the genus Torradovirus by N-terminal sequencing of the virion capsid proteins","volume":"498","author":"Ferriol","year":"2016","journal-title":"Virology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1099\/jgv.0.001807","article-title":"ICTV Report Consortium. ICTV Virus Taxonomy Profile: Secoviridae 2022","volume":"103","author":"Fuchs","year":"2022","journal-title":"J. Gen. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Fontdevila Pareta, N., Khalili, M., Maachi, A., Rivarez, M.P.S., Rollin, J., Salavert, F., Temple, C., Aranda, M.A., Boonham, N., and Botermans, M. (2023). Managing the deluge of newly discovered plant viruses and viroids: An optimized scientific and regulatory framework for their characterization and risk analysis. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1181562"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1093\/bioinformatics\/14.4.380","article-title":"MView: A web-compatible database search or multiple alignment viewer","volume":"14","author":"Brown","year":"1998","journal-title":"Bioinformatics"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/1\/84\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T05:19:08Z","timestamp":1768022348000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/1\/84"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":60,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["v18010084"],"URL":"https:\/\/doi.org\/10.3390\/v18010084","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]}}}