{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T17:23:01Z","timestamp":1769707381116,"version":"3.49.0"},"reference-count":44,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gansu Province Science and Technology Foundation for the Cooperation Program","award":["24JRRA810"],"award-info":[{"award-number":["24JRRA810"]}]},{"name":"Gansu Province Science and Technology Foundation for the Cooperation Program","award":["23JRRA1481"],"award-info":[{"award-number":["23JRRA1481"]}]},{"name":"Basic Research Project of Yazhouwan National Laboratory","award":["JL23YCKY01"],"award-info":[{"award-number":["JL23YCKY01"]}]},{"name":"Key Development and Research Foundation of Gansu","award":["21YF5WA153"],"award-info":[{"award-number":["21YF5WA153"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32202779"],"award-info":[{"award-number":["32202779"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32373027"],"award-info":[{"award-number":["32373027"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Lumpy skin disease virus (LSDV), a Capripoxvirus genus member, causes severe cattle disease. Though Capripoxviruses share high nucleotide sequence homology indicating common ancestry, they have evolved distinct host adaptations. The LSDV genome encodes numerous proteins, with ORF133 being LSDV-specific and lacking clear homologs in other Capripoxviruses, implying potential roles in host range and virulence. To explore ORF133\u2019s function, this study generated mouse polyclonal antibodies against ORF133 and constructed the ORF133-deleted recombinant virus (LSDV\u0394ORF133-EGFP) via homologous recombination with an EGFP reporter. Preliminary characterization showed that ORF133 deletion affects viral replication. This study provides critical tools and theoretical references for subsequent investigations into the functional mechanisms underlying ORF133 in LSDV.<\/jats:p>","DOI":"10.3390\/v18020158","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T08:14:23Z","timestamp":1769415263000},"page":"158","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Construction and Biological Characterization of ORF133-Deletion Mutant of Lumpy Skin Disease Virus"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9290-8742","authenticated-orcid":false,"given":"Qunhua","family":"Ke","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miaomiao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangwei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuefeng","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"Yazhouwan National Laboratory, Sanya 572024, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-1740","authenticated-orcid":false,"given":"Xiangping","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanming","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2222\/jsv.75.1","article-title":"Lumpy Skin Disease","volume":"75","author":"Watanabe","year":"2025","journal-title":"Uirusu"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.prevetmed.2017.09.003","article-title":"Economic impact of lumpy skin disease and cost effectiveness of vaccination for the control of outbreaks in Ethiopia","volume":"147","author":"Molla","year":"2017","journal-title":"Prev. 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