{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T16:30:11Z","timestamp":1769704211761,"version":"3.49.0"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordination for the Improvement of Higher Education Personnel","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Advances in diagnostic techniques, along with environmental changes driven by human activity, have intensified the surveillance and monitoring of virus and arbovirus circulation on the Amazon. These efforts have increased the detection of insect-specific viruses in field-collected hematophagous arthropods. This study reports the first isolation of the Aguas Brancas virus from mosquitoes collected in the Brazilian Amazon and in a rural area of Bras\u00edlia, Federal District, Brazil. Arthropods of the family Culicidae, genus Limatus durhamii, were collected at ground level in forest fragments. Sample BEAR812610 originated from Ananindeua, Par\u00e1, within the Evandro Chagas Institute\u2019s grounds, and sample BEAR839941 from a forest fragment in Bras\u00edlia (Ceil\u00e2ndia\u2014N\u00facleo Rural Boa Esperan\u00e7a, Site B4). Specimens were identified to the species\/genus level, macerated, and the supernatant inoculated into C6\/36 and Vero cell cultures for viral isolation. The presence of arboviruses was determined by indirect immunofluorescence using antibodies against major arbovirus groups. Positive samples were sequenced for nucleotide and amino acid identification, and phylogenetic analysis confirmed the virus as belonging to the genus Orthoflavivirus. This represents the first report of the isolation and characterization of the insect-specific Aguas Brancas virus.<\/jats:p>","DOI":"10.3390\/v18020164","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:05:27Z","timestamp":1769504727000},"page":"164","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Record of Isolation and Molecular Characterization of Aguas Brancas virus, a New Insect-Specific Virus Found in Brazil"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6117-5766","authenticated-orcid":false,"given":"Val\u00e9ria Cardoso","family":"Freitas","sequence":"first","affiliation":[{"name":"Graduate Program in Virology, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7510-6641","authenticated-orcid":false,"given":"F\u00e1bio Silva da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3560-5499","authenticated-orcid":false,"given":"Daniel Damous","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Wilson","family":"Rosa Junior","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8529-4751","authenticated-orcid":false,"given":"Bruna La\u00eds Sena do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maissa Maia","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Leimar Camelo","family":"Silva","sequence":"additional","affiliation":[{"name":"Directorate of Environmental Health Surveillance, State Secretariat of Health of the Federal District, Bras\u00edlia 70655-775, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Raquel Lira","family":"Vieira","sequence":"additional","affiliation":[{"name":"Directorate of Environmental Health Surveillance, State Secretariat of Health of the Federal District, Bras\u00edlia 70655-775, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana Cec\u00edlia Ribeiro","family":"Cruz","sequence":"additional","affiliation":[{"name":"Graduate Program in Virology, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandro Patroca da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8578-9984","authenticated-orcid":false,"given":"Livia Medeiros Neves","family":"Casseb","sequence":"additional","affiliation":[{"name":"Graduate Program in Virology, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5981-0065","authenticated-orcid":false,"given":"Joaquim Pinto","family":"Nunes Neto","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8385-8253","authenticated-orcid":false,"given":"Val\u00e9ria Lima","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Graduate Program in Virology, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"},{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1590\/1806-93472019v39n82-02","article-title":"\u201cUma Floresta Cheia de V\u00edrus!\u201d Ci\u00eancia e Desenvolvimento Nas Fronteiras Amaz\u00f4nicas","volume":"39","year":"2019","journal-title":"Rev. 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