{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:16:44Z","timestamp":1791047804312,"version":"4.1.0"},"reference-count":82,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Allergy and Infectious Diseases, National Institutes of Health","award":["R01AI187430"],"award-info":[{"award-number":["R01AI187430"]}]},{"DOI":"10.13039\/100007199","name":"Utah Agricultural Experiment Station","doi-asserted-by":"publisher","award":["UTA01655"],"award-info":[{"award-number":["UTA01655"]}],"id":[{"id":"10.13039\/100007199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic orthoflavivirus that poses a significant global health threat. It causes severe neuroinflammatory disease in humans and reproductive failure in swine. Because of the broad host range and cell tropism of JEV, identifying animal cell lines resistant to infection has been a persistent challenge. In this study, we demonstrate that Madin\u2013Darby bovine kidney (MDBK) cells are resistant to JEV infection yet remain fully permissive to viral replication when transfected with viral genomic RNA. Using immunoblotting, immunofluorescence, and flow cytometry, we show that MDBK cells, unlike the highly susceptible baby hamster kidney (BHK-21) cells used as controls, do not support viral entry but sustain all post-entry stages of the replication cycle. Further investigation confirmed that MDBK cells possess a functional clathrin-mediated endocytic pathway, as evidenced by their susceptibility to bovine viral diarrhea virus, which relies on clathrin-dependent endocytosis for host cell entry. These findings establish MDBK cells as a nonsusceptible cell line for JEV entry despite intact endocytic function, providing a valuable platform for studying virus\u2013host cell interactions and for identifying and validating host cell entry factors, a major challenge in JEV research.<\/jats:p>","DOI":"10.3390\/v18020166","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T10:27:11Z","timestamp":1769509631000},"page":"166","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Bovine Cell Line Resistant to Japanese Encephalitis Virus Entry but Permissive to Post-Entry Replication"],"prefix":"10.3390","volume":"18","author":[{"given":"Sang-Im","family":"Yun","sequence":"first","affiliation":[{"name":"Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Natural Resources, Utah State University, Logan, UT 84322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3974-3405","authenticated-orcid":false,"given":"Young-Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Natural Resources, Utah State University, Logan, UT 84322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1099\/jgv.0.000672","article-title":"ICTV virus taxonomy profile: Flaviviridae","volume":"98","author":"Simmonds","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2814","DOI":"10.1080\/21505594.2021.1996059","article-title":"Pathogenesis and virulence of flavivirus infections","volume":"12","author":"Heunis","year":"2021","journal-title":"Virulence"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Frank, J.C., Song, B.H., and Lee, Y.M. (2023). Mice as an animal model for Japanese encephalitis virus research: Mouse susceptibility, infection route, and viral pathogenesis. Pathogens, 12.","DOI":"10.3390\/pathogens12050715"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.vetmic.2017.01.014","article-title":"Japanese encephalitis virus infection, diagnosis and control in domestic animals","volume":"201","author":"Mansfield","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"860","DOI":"10.4269\/ajtmh.23-0036","article-title":"Japanese encephalitis among adults: A review","volume":"108","author":"Hills","year":"2023","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Cheng, Y., Tran Minh, N., Tran Minh, Q., Khandelwal, S., and Clapham, H.E. (2022). Estimates of Japanese encephalitis mortality and morbidity: A systematic review and modeling analysis. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010361"},{"key":"ref_7","unstructured":"Scheld, W.M., Hammer, S.M., and Hughes, J.M. (2008). Japanese encephalitis prevention and control: Advances, challenges, and new initiatives. Emerging Infections 8, ASM Press."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1534114","DOI":"10.3389\/fvets.2025.1534114","article-title":"Prevalence of Japanese encephalitis in pigs in Mainland China during 2000\u20132024: A systemic review and meta-analysis","volume":"12","author":"Liu","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Park, S.L., Huang, Y.S., and Vanlandingham, D.L. (2022). Re-examining the importance of pigs in the transmission of Japanese encephalitis virus. Pathogens, 11.","DOI":"10.3390\/pathogens11050575"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ladreyt, H., Durand, B., Dussart, P., and Chevalier, V. (2019). How central is the domestic pig in the epidemiological cycle of Japanese encephalitis virus? A review of scientific evidence and implications for disease control. Viruses, 11.","DOI":"10.3390\/v11100949"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1262\/jrd1977.23.171","article-title":"Reproductive disorders in boars infected experimentally with Japanese encephalitis virus","volume":"23","author":"Ogasa","year":"1977","journal-title":"Jpn. J. Anim. Reprod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/s41564-020-0714-0","article-title":"The continued threat of emerging flaviviruses","volume":"5","author":"Pierson","year":"2020","journal-title":"Nat. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1146\/annurev.ento.54.110807.090510","article-title":"Ecology and geographical expansion of Japanese encephalitis virus","volume":"54","author":"Ritchie","year":"2009","journal-title":"Annu. Rev. Entomol."},{"key":"ref_14","first-page":"205","article-title":"Japanese encephalitis: On the One Health agenda","volume":"365","author":"Impoinvil","year":"2013","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/zph.13103","article-title":"Potential for emergence of Japanese encephalitis in the European Union","volume":"71","author":"Gossner","year":"2024","journal-title":"Zoonoses Public Health"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Logiudice, J., Alberti, M., Ciccarone, A., Rossi, B., Tiecco, G., De Francesco, M.A., and Quiros-Roldan, E. (2025). Introduction of vector-borne infections in Europe: Emerging and re-emerging viral pathogens with potential impact on One Health. Pathogens, 14.","DOI":"10.3390\/pathogens14010063"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1056\/NEJMc1701600","article-title":"Autochthonous Japanese encephalitis with yellow fever coinfection in Africa","volume":"376","author":"Faye","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100879","DOI":"10.1016\/j.onehlt.2024.100879","article-title":"Revisiting the risk of introduction of Japanese encephalitis virus (JEV) into the United States\u2014An updated semi-quantitative risk assessment","volume":"19","author":"Dixon","year":"2024","journal-title":"One Health"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1038\/nrneurol.2018.30","article-title":"Japanese encephalitis\u2014The prospects for new treatments","volume":"14","author":"Turtle","year":"2018","journal-title":"Nat. Rev. Neurol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"263","DOI":"10.4161\/hv.26902","article-title":"Japanese encephalitis: The virus and vaccines","volume":"10","author":"Yun","year":"2014","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1080\/21645515.2017.1285472","article-title":"Japanese encephalitis vaccines: Immunogenicity, protective efficacy, effectiveness, and impact on the burden of disease","volume":"13","author":"Hegde","year":"2017","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e51027","DOI":"10.7554\/eLife.51027","article-title":"Estimates of the global burden of Japanese encephalitis and the impact of vaccination from 2000\u20132015","volume":"9","author":"Quan","year":"2020","journal-title":"eLife"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"S98","DOI":"10.1038\/nm1144","article-title":"Emerging flaviviruses: The spread and resurgence of Japanese encephalitis, West Nile and dengue viruses","volume":"10","author":"Mackenzie","year":"2004","journal-title":"Nat. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"77","DOI":"10.4103\/0972-9062.117470","article-title":"Emerging and re-emerging arboviral diseases in Southeast Asia","volume":"50","author":"Dash","year":"2013","journal-title":"J. Vector Borne Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Tripathi, A., Chauhan, S., and Khasa, R. (2025). A comprehensive review of the development and therapeutic use of antivirals in flavivirus infection. Viruses, 17.","DOI":"10.3390\/v17010074"},{"key":"ref_26","first-page":"583","article-title":"Japanese encephalitis virus (Flaviviridae)","volume":"Volume 2","author":"Bamford","year":"2021","journal-title":"Encyclopedia of Virology"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Poonsiri, T., Wright, G.S.A., Solomon, T., and Antonyuk, S.V. (2019). Crystal structure of the Japanese encephalitis virus capsid protein. Viruses, 11.","DOI":"10.3390\/v11070623"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1038\/s41467-017-00024-6","article-title":"Near-atomic structure of Japanese encephalitis virus reveals critical determinants of virulence and stability","volume":"8","author":"Wang","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Yun, S.I., and Lee, Y.M. (2018). Early events in Japanese encephalitis virus infection: Viral entry. Pathogens, 7.","DOI":"10.3390\/pathogens7030068"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"199120","DOI":"10.1016\/j.virusres.2023.199120","article-title":"Virus-host interactions in early Japanese encephalitis virus infection","volume":"331","author":"Zhu","year":"2023","journal-title":"Virus Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kim, J.K., Kim, J.M., Song, B.H., Yun, S.I., Yun, G.N., Byun, S.J., and Lee, Y.M. (2015). Profiling of viral proteins expressed from the genomic RNA of Japanese encephalitis virus using a panel of 15 region-specific polyclonal rabbit antisera: Implications for viral gene expression. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0124318"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1743-422X-6-14","article-title":"A conserved predicted pseudoknot in the NS2A-encoding sequence of West Nile and Japanese encephalitis flaviviruses suggests NS1\u2019 may derive from ribosomal frameshifting","volume":"6","author":"Firth","year":"2009","journal-title":"Virol. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1128\/JVI.01979-09","article-title":"NS1\u2019 of flaviviruses in the Japanese encephalitis virus serogroup is a product of ribosomal frameshifting and plays a role in viral neuroinvasiveness","volume":"84","author":"Melian","year":"2010","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"10438","DOI":"10.1128\/JVI.00986-10","article-title":"The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex","volume":"84","author":"Gillespie","year":"2010","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/nrmicro.2017.170","article-title":"Rewiring cellular networks by members of the Flaviviridae family","volume":"16","author":"Neufeldt","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Morita, E., and Suzuki, Y. (2021). Membrane-associated flavivirus replication complex\u2014Its organization and regulation. Viruses, 13.","DOI":"10.3390\/v13061060"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"van den Elsen, K., Quek, J.P., and Luo, D. (2021). Molecular insights into the flavivirus replication complex. Viruses, 13.","DOI":"10.3390\/v13060956"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.tibs.2020.12.007","article-title":"Molecular determinants of flavivirus virion assembly","volume":"46","author":"Barnard","year":"2021","journal-title":"Trends Biochem. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1038\/s41467-020-14647-9","article-title":"Capsid protein structure in Zika virus reveals the flavivirus assembly process","volume":"11","author":"Tan","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1830","DOI":"10.1126\/science.1153263","article-title":"The flavivirus precursor membrane-envelope protein complex: Structure and maturation","volume":"319","author":"Li","year":"2008","journal-title":"Science"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2604","DOI":"10.1093\/emboj\/cdg270","article-title":"Structures of immature flavivirus particles","volume":"22","author":"Zhang","year":"2003","journal-title":"EMBO J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"8475","DOI":"10.1128\/jvi.71.11.8475-8481.1997","article-title":"Proteolytic activation of tick-borne encephalitis virus by furin","volume":"71","author":"Stadler","year":"1997","journal-title":"J. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1126\/science.1153264","article-title":"Structure of the immature dengue virus at low pH primes proteolytic maturation","volume":"319","author":"Yu","year":"2008","journal-title":"Science"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.coviro.2012.02.011","article-title":"Degrees of maturity: The complex structure and biology of flaviviruses","volume":"2","author":"Pierson","year":"2012","journal-title":"Curr. Opin. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4448","DOI":"10.1021\/acs.chemrev.7b00719","article-title":"Biochemistry and molecular biology of flaviviruses","volume":"118","author":"Barrows","year":"2018","journal-title":"Chem. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Song, B.H., Yun, S.I., Goldhardt, J.L., Kim, J., and Lee, Y.M. (2025). Key virulence factors responsible for differences in pathogenicity between clinically proven live-attenuated Japanese encephalitis vaccine SA14-14-2 and its pre-attenuated highly virulent parent SA14. PLoS Pathog., 21.","DOI":"10.1371\/journal.ppat.1012844"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.1128\/JVI.79.6.3231-3242.2005","article-title":"Dual mechanisms of pestiviral superinfection exclusion at entry and RNA replication","volume":"79","author":"Lee","year":"2005","journal-title":"J. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6450","DOI":"10.1128\/JVI.77.11.6450-6465.2003","article-title":"Development and application of a reverse genetics system for Japanese encephalitis virus","volume":"77","author":"Yun","year":"2003","journal-title":"J. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"7846","DOI":"10.1128\/JVI.00789-08","article-title":"A single N-linked glycosylation site in the Japanese encephalitis virus prM protein is critical for cell type-specific prM protein biogenesis, virus particle release, and pathogenicity in mice","volume":"82","author":"Kim","year":"2008","journal-title":"J. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4737","DOI":"10.1128\/JVI.72.6.4737-4745.1998","article-title":"Infectious bovine viral diarrhea virus (strain NADL) RNA from stable cDNA clones: A cellular insert determines NS3 production and viral cytopathogenicity","volume":"72","author":"Mendez","year":"1998","journal-title":"J. Virol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1099\/jgv.0.000574","article-title":"Comparison of the live-attenuated Japanese encephalitis vaccine SA14-14-2 strain with its pre-attenuated virulent parent SA14 strain: Similarities and differences in vitro and in vivo","volume":"97","author":"Yun","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Yun, S.I., Song, B.H., Kim, J.K., Yun, G.N., Lee, E.Y., Li, L., Kuhn, R.J., Rossmann, M.G., Morrey, J.D., and Lee, Y.M. (2014). A molecularly cloned, live-attenuated Japanese encephalitis vaccine SA14-14-2 virus: A conserved single amino acid in the ij hairpin of the viral E glycoprotein determines neurovirulence in mice. PLoS Pathog., 10, Erratum in PLoS Pathog. 2014, 10, e1004465. https:\/\/doi.org\/10.1371\/journal.ppat.1004465.","DOI":"10.1371\/journal.ppat.1004465"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e01113-17","DOI":"10.1128\/JVI.01113-17","article-title":"Rab5 and Rab11 are required for clathrin-dependent endocytosis of Japanese encephalitis virus in BHK-21 cells","volume":"91","author":"Liu","year":"2017","journal-title":"J. Virol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"10826","DOI":"10.1128\/JVI.79.16.10826-10829.2005","article-title":"Bovine viral diarrhea virus entry is dependent on clathrin-mediated endocytosis","volume":"79","author":"Lecot","year":"2005","journal-title":"J. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4191","DOI":"10.1128\/JVI.79.7.4191-4200.2005","article-title":"Acid-resistant bovine pestivirus requires activation for pH-triggered fusion during entry","volume":"79","author":"Krey","year":"2005","journal-title":"J. Virol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/j.1439-0450.2004.00798.x","article-title":"Bovine viral diarrhoea virus is internalized by clathrin-dependent receptor-mediated endocytosis","volume":"51","author":"Grummer","year":"2004","journal-title":"J. Vet. Med. B Infect. Dis. Vet. Public Health"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.mi.40.100186.002143","article-title":"The natural history of Japanese encephalitis virus","volume":"40","author":"Rosen","year":"1986","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/jvi.68.1.1-5.1994","article-title":"Virus receptors: Binding, adhesion strengthening, and changes in viral structure","volume":"68","author":"Haywood","year":"1994","journal-title":"J. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/1743-422X-8-115","article-title":"Identification of a mutated BHK-21 cell line that became less susceptible to Japanese encephalitis virus infection","volume":"8","author":"Ding","year":"2011","journal-title":"Virol. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/BF01310788","article-title":"Analysis of virus-cell binding characteristics on the determination of Japanese encephalitis virus susceptibility","volume":"139","author":"Kimura","year":"1994","journal-title":"Arch. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1111\/1348-0421.12085","article-title":"Characterization of Japanese encephalitis virus infection in an immortalized mesencephalic cell line, CSM14.1","volume":"57","author":"Kimura","year":"2013","journal-title":"Microbiol. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1006\/biol.1996.0031","article-title":"Establishment and characterization of Japanese B encephalitis virus persistent infection in the Sf9 insect cell line","volume":"24","author":"Fu","year":"1996","journal-title":"Biologicals"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"9844","DOI":"10.1128\/JVI.72.12.9844-9854.1998","article-title":"Antiapoptotic but not antiviral function of human bcl-2 assists establishment of Japanese encephalitis virus persistence in cultured cells","volume":"72","author":"Liao","year":"1998","journal-title":"J. Virol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1128\/jvi.24.2.580-589.1977","article-title":"Persistent infection of cultured mammalian cells by Japanese encephalitis virus","volume":"24","author":"Schmaljohn","year":"1977","journal-title":"J. Virol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Adetunji, S.A., Smolensky, D., Mitzel, D.N., Owens, J.L., Chitko-McKown, C.G., Cernicchiaro, N., and Noronha, L.E. (2021). In vitro infection dynamics of Japanese encephalitis virus in established porcine cell lines. Pathogens, 10.","DOI":"10.3390\/pathogens10111468"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/BF01309728","article-title":"Japanese encephalitis virus infection of mouse cell lines: Ability to prime mice for generation of virus specific cytotoxic T lymphocytes and differences in CTL recognisable viral determinants","volume":"140","author":"Ravi","year":"1995","journal-title":"Arch. Virol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1099\/vir.0.19426-0","article-title":"A model to study neurotropism and persistency of Japanese encephalitis virus infection in human neuroblastoma cells and leukocytes","volume":"85","author":"Yang","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_68","first-page":"157","article-title":"Experimental studies on the susceptibility of domestic pigs to West Nile virus followed by Japanese encephalitis virus infection and vice versa","volume":"38","author":"Ilkal","year":"1994","journal-title":"Acta Virol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Le Flohic, G., Porphyre, V., Barbazan, P., and Gonzalez, J.P. (2013). Review of climate, landscape, and viral genetics as drivers of the Japanese encephalitis virus ecology. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002208"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"698","DOI":"10.4269\/ajtmh.1959.8.698","article-title":"Ecologic studies of Japanese encephalitis virus in Japan. VI. Swine infection","volume":"8","author":"Scherer","year":"1959","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"620","DOI":"10.4049\/jimmunol.83.6.620","article-title":"Immunologic studies of Japanese encephalitis virus in Japan. V. Maternal antibodies, antibody responses and viremia following infection of swine","volume":"83","author":"Scherer","year":"1959","journal-title":"J. Immunol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"621","DOI":"10.3181\/00379727-75-18285","article-title":"Congenital Japanese B encephalitis infection of swine","volume":"75","author":"Burns","year":"1950","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"420","DOI":"10.4269\/ajtmh.1988.38.420","article-title":"Ecological studies of Japanese encephalitis virus in Hokkaido: Interepidemic outbreaks of swine abortion and evidence for the virus to overwinter locally","volume":"38","author":"Takashima","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"10832","DOI":"10.1038\/ncomms10832","article-title":"Vector-free transmission and persistence of Japanese encephalitis virus in pigs","volume":"7","author":"Ricklin","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s13567-016-0319-z","article-title":"Japanese encephalitis virus tropism in experimentally infected pigs","volume":"47","author":"Ricklin","year":"2016","journal-title":"Vet. Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3448","DOI":"10.1128\/JCM.00737-13","article-title":"Nonsuppurative encephalomyelitis in a calf in Japan and isolation of Japanese encephalitis virus genotype 1 from the affected calf","volume":"51","author":"Katayama","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Kako, N., Suzuki, S., Sugie, N., Kato, T., Yanase, T., Yamakawa, M., and Shirafuji, H. (2014). Japanese encephalitis in a 114-month-old cow: Pathological investigation of the affected cow and genetic characterization of Japanese encephalitis virus isolate. BMC Vet. Res., 10.","DOI":"10.1186\/1746-6148-10-63"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1016\/0035-9203(88)90536-6","article-title":"Absence of viraemia in cattle after experimental infection with Japanese encephalitis virus","volume":"82","author":"Ilkal","year":"1988","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.virol.2008.06.017","article-title":"Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein","volume":"379","author":"Chien","year":"2008","journal-title":"Virology"},{"key":"ref_80","first-page":"242","article-title":"Sero-positivity of domestic animals against Japanese encephalitis in Bareilly area, U.P","volume":"27","author":"Mall","year":"1995","journal-title":"J. Commun. Dis."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Levesque, Z.A., Walsh, M.G., Webb, C.E., Zadoks, R.N., and Brookes, V.J. (2024). A scoping review of evidence of naturally occurring Japanese encephalitis infection in vertebrate animals other than humans, ardeid birds and pigs. PLoS Negl. Trop. Dis., 18.","DOI":"10.1101\/2024.04.18.589998"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1186\/s13071-017-2354-7","article-title":"Meta-analyses of the proportion of Japanese encephalitis virus infection in vectors and vertebrate hosts","volume":"10","author":"Oliveira","year":"2017","journal-title":"Parasit. Vectors"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/2\/166\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T10:31:12Z","timestamp":1769509872000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/2\/166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":82,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["v18020166"],"URL":"https:\/\/doi.org\/10.3390\/v18020166","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]}}}