{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:07:10Z","timestamp":1790399230551,"version":"4.1.0"},"reference-count":319,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Following the COVID-19 pandemic, researchers have increasingly focused on monitoring the spread of the virus and improving methods to detect changes in the SARS-CoV-2 genome. Although clinical surveillance provides direct and reliable results, it has limited applicability. Wastewater-based epidemiology (WBE) has therefore emerged as a valuable, non-invasive complementary tool for disease surveillance. It provides a comprehensive picture of virus circulation in a population, including asymptomatic individuals and those who do not seek healthcare. In addition, it facilitates early detection of outbreaks and the collection of epidemiologic data at the community level. However, WBE also presents technical challenges, including variations in sampling and testing protocols, the presence of inhibitors that affect viral RNA extraction, and the need for standardised procedures between studies. These challenges should be addressed for possible future infectious disease outbreaks. One of the challenges facing researchers was to develop efficient methods that could overcome the extraction and detection problems related to inhibitors present in wastewater. To this aim, this systematic review highlights the potential use of WBE, the variety of techniques, and the most effective methods for the detection and quantification of SARS-CoV-2 in wastewater samples. A reproducible electronic search of the literature was conducted in the Web of Science (WoS) and PubMed databases for articles published between 2020 and 2024. Our search revealed that the majority of observed WBE applications emphasised a correlation between SARS-CoV-2 RNA concentration trends in wastewater and epidemiological data. Another relevant issue that the articles often discussed and compared was the techniques used in different steps of sample processing, such as sample collection, concentration and detection, hence the lack of standardised procedures. This paper provides a framework regarding previous research on WBE to gain a better understanding that will lead to functional solutions.<\/jats:p>","DOI":"10.3390\/v18020205","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T13:56:23Z","timestamp":1770213383000},"page":"205","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4211-8504","authenticated-orcid":false,"given":"Gy\u00f6rgy","family":"De\u00e1k","sequence":"first","affiliation":[{"name":"National Institute for Research and Development in Environmental Protection, Splaiul Independen\u0163ei 294, 060031 Bucharest, Romania"},{"name":"Doctoral School of Biotechnical Systems Engineering, National University of Science and Technology POLITEHNICA of Bucharest, Splaiul Independen\u021bei 313, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1755-627X","authenticated-orcid":false,"given":"Laura","family":"Lupu","sequence":"additional","affiliation":[{"name":"National Institute for Research and Development in Environmental Protection, Splaiul Independen\u0163ei 294, 060031 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0715-5025","authenticated-orcid":false,"given":"Raluca","family":"Prangate","sequence":"additional","affiliation":[{"name":"National Institute for Research and Development in Environmental Protection, Splaiul Independen\u0163ei 294, 060031 Bucharest, Romania"},{"name":"Doctoral School of Biotechnical Systems Engineering, National University of Science and Technology POLITEHNICA of Bucharest, Splaiul Independen\u021bei 313, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1056\/NEJMoa2001017","article-title":"A Novel Coronavirus from Patients with Pneumonia in China, 2019","volume":"382","author":"Zhu","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/s41586-020-2008-3","article-title":"A new coronavirus associated with human respiratory disease in China","volume":"579","author":"Wu","year":"2020","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mahdy, M.A.A., Younis, W., and Ewaida, Z. (2020). An Overview of SARS-CoV-2 and Animal Infection. Front. Veter-Sci., 7.","DOI":"10.3389\/fvets.2020.596391"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/s41579-020-00459-7","article-title":"Characteristics of SARS-CoV-2 and COVID-19","volume":"19","author":"Hu","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105924","DOI":"10.1016\/j.ijantimicag.2020.105924","article-title":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): The epidemic and the challenges","volume":"55","author":"Lai","year":"2020","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1002\/jmv.25766","article-title":"Coronavirus disease 2019: What we know?","volume":"92","author":"He","year":"2020","journal-title":"J. Med. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1038\/s41564-020-0695-z","article-title":"The species Severe acute respiratory syndrome-related coronavirus: Classifying 2019-nCoV and naming it SARS-CoV-2","volume":"5","author":"Gorbalenya","year":"2020","journal-title":"Nat. Microbiol."},{"key":"ref_8","first-page":"157","article-title":"WHO Declares COVID-19 a Pandemic","volume":"91","author":"Cucinotta","year":"2020","journal-title":"Acta Biomed. Atenei Parm."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3345\/cep.2020.00493","article-title":"Epidemiology, virology, and clinical features of severe acute respiratory syndrome -coronavirus-2 (SARS-CoV-2; Coronavirus Disease-19)","volume":"63","author":"Park","year":"2020","journal-title":"Clin. Exp. Pediatr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"317","DOI":"10.2144\/btn-2020-0057","article-title":"PCR past, present and future","volume":"69","author":"Zhu","year":"2020","journal-title":"BioTechniques"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"166725","DOI":"10.1016\/j.jmb.2020.11.024","article-title":"Structural insights into SARS-CoV-2 proteins","volume":"433","author":"Arya","year":"2021","journal-title":"J. Mol. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/s41579-020-00468-6","article-title":"Coronavirus biology and replication: Implications for SARS-CoV-2","volume":"19","author":"Kratzel","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1021\/acs.estlett.0c00357","article-title":"Presence of SARS-Coronavirus-2 RNA in Sewage and Correlation with Reported COVID-19 Prevalence in the Early Stage of the Epidemic in The Netherlands","volume":"7","author":"Medema","year":"2020","journal-title":"Environ. Sci. Technol. Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"112204","DOI":"10.1016\/j.envres.2021.112204","article-title":"Coronaviruses and SARS-CoV-2 in sewerage and their removal: Step by step in wastewater treatment plants","volume":"207","author":"Foladori","year":"2022","journal-title":"Environ. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"S294","DOI":"10.1093\/infdis\/jiu384","article-title":"Environmental Surveillance for Polioviruses in the Global Polio Eradication Initiative","volume":"210","author":"Asghar","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/s44221-023-00083-8","article-title":"A world of wastewater-based epidemiology","volume":"1","author":"Singer","year":"2023","journal-title":"Nat. Water"},{"key":"ref_17","unstructured":"European Commission (2023, December 05). Commission Recommendation (EU) 2021\/472 of 17 March 2021 on a Common Approach to Establish a Systematic Surveillance of SARS-CoV-2 and Its Variants in Wastewaters in the EU. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/PDF\/?uri=CELEX:32021H0472."},{"key":"ref_18","first-page":"973","article-title":"Development of a methodology for monitoring SARS-CoV-2 RNA in wastewater","volume":"13","author":"Matei","year":"2022","journal-title":"Int. J. Conserv. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"21730","DOI":"10.1038\/s41598-024-72854-6","article-title":"The first detection of SARS-CoV-2 RNA in the wastewater of Bucharest, Romania","volume":"14","author":"Prangate","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1038\/s41587-020-0684-z","article-title":"Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics","volume":"38","author":"Peccia","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S2468-1253(20)30087-X","article-title":"SARS-CoV-2 in wastewater: Potential health risk, but also data source","volume":"5","author":"Lodder","year":"2020","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"138764","DOI":"10.1016\/j.scitotenv.2020.138764","article-title":"First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community","volume":"728","author":"Ahmed","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Bar-Or, I., Indenbaum, V., Weil, M., Elul, M., Levi, N., Aguvaev, I., Cohen, Z., Levy, V., Azar, R., and Mannasse, B. (2022). National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel. Viruses, 14.","DOI":"10.3390\/v14061229"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e02703-20","DOI":"10.1128\/mBio.02703-20","article-title":"Genome Sequencing of Sewage Detects Regionally Prevalent SARS-CoV-2 Variants","volume":"12","author":"Kantor","year":"2021","journal-title":"mBio"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100536","DOI":"10.1016\/j.onehlt.2023.100536","article-title":"Significance of wastewater surveillance in detecting the prevalence of SARS-CoV-2 variants and other respiratory viruses in the community\u2014A multi-site evaluation","volume":"16","author":"Khan","year":"2023","journal-title":"One Health"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1016\/j.joi.2016.10.006","article-title":"Constructing bibliometric networks: A comparison between full and fractional counting","volume":"10","author":"Waltman","year":"2016","journal-title":"J. Informetr."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Laicans, J., Dejus, B., Dejus, S., and Juhna, T. (2024). Precision and Accuracy Limits of Wastewater-Based Epidemiology\u2014Lessons Learned from SARS-CoV-2: A Scoping Review. Water, 16.","DOI":"10.3390\/w16091220"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2000045","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Eurosurveillance"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Quer, J., Colomer-Castell, S., Campos, C., Andr\u00e9s, C., Pi\u00f1ana, M., Cortese, M.F., Gonz\u00e1lez-S\u00e1nchez, A., Garcia-Cehic, D., Ib\u00e1\u00f1ez, M., and Pumarola, T. (2022). Next-Generation Sequencing for Confronting Virus Pandemics. Viruses, 14.","DOI":"10.3390\/v14030600"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"140910","DOI":"10.1016\/j.scitotenv.2020.140910","article-title":"Viruses in wastewater: Occurrence, abundance and detection methods","volume":"745","author":"Corpuz","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.coesh.2020.01.006","article-title":"Recent trends on methods for the concentration of viruses from water samples","volume":"16","year":"2020","journal-title":"Curr. Opin. Environ. Sci. Health"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"141245","DOI":"10.1016\/j.scitotenv.2020.141245","article-title":"Primary concentration\u2014The critical step in implementing the wastewater based epidemiology for the COVID-19 pandemic: A mini-review","volume":"747","author":"Lu","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"100214","DOI":"10.1016\/j.cscee.2022.100214","article-title":"Evaluation of SARS-CoV-2 concentrations in wastewater and river water samples","volume":"6","author":"Fonseca","year":"2022","journal-title":"Case Stud. Chem. Environ. Eng."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"142929","DOI":"10.1016\/j.scitotenv.2020.142929","article-title":"Detection and quantification of SARS-CoV-2 RNA in wastewater and treated effluents: Surveillance of COVID-19 epidemic in the United Arab Emirates","volume":"764","author":"Hasan","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"139076","DOI":"10.1016\/j.scitotenv.2020.139076","article-title":"SARS-CoV-2 in wastewater: State of the knowledge and research needs","volume":"739","author":"Kitajima","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1111\/1751-7915.13989","article-title":"Monitoring COVID-19 through SARS-CoV-2 quantification in wastewater: Progress, challenges and prospects","volume":"15","author":"Alhama","year":"2022","journal-title":"Microb. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"140405","DOI":"10.1016\/j.scitotenv.2020.140405","article-title":"First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan","volume":"737","author":"Haramoto","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.coesh.2020.01.007","article-title":"Emerging technologies for the rapid detection of enteric viruses in the aquatic environment","volume":"16","author":"Farkas","year":"2020","journal-title":"Curr. Opin. Environ. Sci. Health"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1021\/acsestwater.1c00387","article-title":"Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater","volume":"2","author":"Ahmed","year":"2022","journal-title":"ACS EST Water"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"162953","DOI":"10.1016\/j.scitotenv.2023.162953","article-title":"Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling","volume":"878","author":"Ciannella","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1093\/bib\/bbaa297","article-title":"Next generation sequencing of SARS-CoV-2 genomes: Challenges, applications and opportunities","volume":"22","author":"Chiara","year":"2021","journal-title":"Brief. Bioinform."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"845","DOI":"10.3390\/cimb43020061","article-title":"Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology","volume":"43","author":"John","year":"2021","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4697","DOI":"10.1016\/j.csbj.2023.09.044","article-title":"Applying the digital data and the bioinformatics tools in SARS-CoV-2 research","volume":"21","author":"Tan","year":"2023","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Lombardi, A., Voli, A., Mancusi, A., Girardi, S., Proroga, Y.T.R., Pierri, B., Olivares, R., Cossentino, L., Suffredini, E., and Rosa, G.L. (2023). SARS-CoV-2 RNA in Wastewater and Bivalve Mollusk Samples of Campania, Southern Italy. Viruses, 15.","DOI":"10.3390\/v15081777"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"157101","DOI":"10.1016\/j.scitotenv.2022.157101","article-title":"The Efficient and Practical virus Identification System with ENhanced Sensitivity for Solids (EPISENS-S): A rapid and cost-effective SARS-CoV-2 RNA detection method for routine wastewater surveillance","volume":"843","author":"Ando","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s00203-023-03417-y","article-title":"Environmental surveillance of SARS-CoV-2 in municipal wastewater to monitor COVID-19 status in urban clusters in Malaysia","volume":"205","author":"Aziz","year":"2023","journal-title":"Arch. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"xtad004","DOI":"10.1093\/femsmc\/xtad004","article-title":"Direct wastewater extraction as a simple and effective method for SARS-CoV-2 surveillance and COVID-19 community-level monitoring","volume":"4","author":"Lott","year":"2023","journal-title":"FEMS Microbes"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"167443","DOI":"10.1016\/j.scitotenv.2023.167443","article-title":"Targeted community wastewater surveillance for SARS-CoV-2 and Mpox virus during a festival mass-gathering event","volume":"906","author":"Brighton","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1021\/acsestwater.1c00160","article-title":"Evaluation of Sampling, Analysis, and Normalization Methods for SARS-CoV-2 Concentrations in Wastewater to Assess COVID-19 Burdens in Wisconsin Communities","volume":"1","author":"Feng","year":"2021","journal-title":"ACS EST Water"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"142867","DOI":"10.1016\/j.scitotenv.2020.142867","article-title":"The wastewater microbiome: A novel insight for COVID-19 surveillance","volume":"764","author":"Benaventel","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"67260","DOI":"10.1007\/s11356-022-20609-z","article-title":"\u201cpySewage\u201d: A hybrid approach to predict the number of SARS-CoV-2-infected people from wastewater in Brazil","volume":"29","author":"Silva","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e0220721","DOI":"10.1128\/spectrum.02207-21","article-title":"Wastewater-Based SARS-CoV-2 Surveillance in Northern New England","volume":"10","author":"Toledo","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e0059122","DOI":"10.1128\/mbio.00591-22","article-title":"Microbiome Analysis for Wastewater Surveillance during COVID-19","volume":"13","author":"Brumfield","year":"2022","journal-title":"mBio"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"112967","DOI":"10.1016\/j.envres.2022.112967","article-title":"Acquired insights from the long-term surveillance of SARS-CoV-2 RNA for COVID-19 monitoring: The case of Monterrey Metropolitan Area (Mexico)","volume":"210","author":"Mora","year":"2022","journal-title":"Environ. Res."},{"key":"ref_56","unstructured":"National Wastewater Surveillance System (2023, September 25). Wastewater Surveillance Testing Methods, Available online: https:\/\/archive.cdc.gov\/#\/details?url=https:\/\/www.cdc.gov\/nwss\/testing.html."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Herold, M., d\u2019H\u00e9rou\u00ebl, A.F., May, P., Delogu, F., Wienecke-Baldacchino, A., Tapp, J., Walczak, C., Wilmes, P., Cauchie, H.-M., and Fournier, G. (2021). Genome Sequencing of SARS-CoV-2 Allows Monitoring of Variants of Concern through Wastewater. Water, 13.","DOI":"10.3390\/w13213018"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"159401","DOI":"10.1016\/j.scitotenv.2022.159401","article-title":"Persistence of endogenous RNA biomarkers of SARS-CoV-2 and PMMoV in raw wastewater: Impact of temperature and implications for wastewater-based epidemiology","volume":"857","author":"Burnet","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Murni, I.K., Oktaria, V., McCarthy, D.T., Supriyati, E., Nuryastuti, T., Handley, A., Donato, C.M., Wiratama, B.S., Dinari, R., and Laksono, I.S. (2024). Wastewater-based epidemiology surveillance as an early warning system for SARS-CoV-2 in Indonesia. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0307364"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"113621","DOI":"10.1016\/j.ijheh.2020.113621","article-title":"Metropolitan wastewater analysis for COVID-19 epidemiological surveillance","volume":"230","author":"Randazzo","year":"2020","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"163454","DOI":"10.1016\/j.scitotenv.2023.163454","article-title":"Near full-automation of COPMAN using a LabDroid enables high-throughput and sensitive detection of SARS-CoV-2 RNA in wastewater as a leading indicator","volume":"881","author":"Hayase","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"146967","DOI":"10.1016\/j.scitotenv.2021.146967","article-title":"Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters","volume":"783","author":"Micsinai","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"117534","DOI":"10.1016\/j.watres.2021.117534","article-title":"Long-term monitoring of SARS-COV-2 RNA in wastewater in Brazil: A more responsive and economical approach","volume":"203","author":"Claro","year":"2021","journal-title":"Water Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2324502","DOI":"10.1080\/22221751.2024.2324502","article-title":"Surveillance of SARS-CoV-2 in wastewater by quantitative PCR and digital PCR: A case study in Shijiazhuang city, Hebei province, China","volume":"13","author":"Chai","year":"2024","journal-title":"Emerg. Microbes Infect."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Martins, R.M., Carvalho, T., Bittar, C., Quevedo, D.M., Miceli, R.N., Nogueira, M.L., Ferreira, H.L., Costa, P.I., Ara\u00fajo, J.P., and Spilki, F.R. (2022). Long-Term Wastewater Surveillance for SARS-CoV-2: One-Year Study in Brazil. Viruses, 14.","DOI":"10.3390\/v14112333"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Lambisia, A.W., Mohammed, K.S., Makori, T.O., Ndwiga, L., Mburu, M.W., Morobe, J.M., Moraa, E.O., Musyoki, J., Murunga, N., and Mwangi, J.N. (2022). Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing Protocol. Front. Med., 9.","DOI":"10.3389\/fmed.2022.836728"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e0064921","DOI":"10.1128\/JCM.00649-21","article-title":"Whole-Genome Sequencing of SARS-CoV-2: Assessment of the Ion Torrent AmpliSeq Panel and Comparison with the Illumina MiSeq ARTIC Protocol","volume":"59","author":"Plitnick","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1038\/s41564-022-01185-x","article-title":"Early detection and surveillance of SARS-CoV-2 genomic variants in wastewater using COJAC","volume":"7","author":"Jahn","year":"2022","journal-title":"Nat. Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.3201\/eid2705.204410","article-title":"Monitoring SARS-CoV-2 Circulation and Diversity through Community Wastewater Sequencing, the Netherlands and Belgium","volume":"27","author":"Elsinga","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"117214","DOI":"10.1016\/j.watres.2021.117214","article-title":"Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK","volume":"200","author":"Hillary","year":"2021","journal-title":"Water Res."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Hassard, F., Vu, M., Rahimzadeh, S., Castro-Gutierrez, V., Stanton, I., Burczynska, B., Wildeboer, D., Baio, G., Brown, M.R., and Garelick, H. (2023). Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0286259"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"067010","DOI":"10.1289\/EHP10289","article-title":"Evaluation of a Wastewater-Based Epidemiological Approach to Estimate the Prevalence of SARS-CoV-2 Infections and the Detection of Viral Variants in Disparate Oregon Communities at City and Neighborhood Scales","volume":"130","author":"Layton","year":"2022","journal-title":"Environ. Health Perspect."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Tangwangvivat, R., Wacharapluesadee, S., Pinyopornpanish, P., Petcharat, S., Hearn, S.M., Thippamom, N., Phiancharoen, C., Hirunpatrawong, P., Duangkaewkart, P., and Supataragul, A. (2023). SARS-CoV-2 Variants Detection Strategies in Wastewater Samples Collected in the Bangkok Metropolitan Region. Viruses, 15.","DOI":"10.3390\/v15040876"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1038\/s41587-022-01387-y","article-title":"Viral variant-resolved wastewater surveillance of SARS-CoV-2 at national scale","volume":"40","author":"Amman","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"100038","DOI":"10.1016\/j.glmedi.2023.100038","article-title":"Addressing language inequities in global health science scholarly publishing","volume":"2","author":"Adebisi","year":"2024","journal-title":"J. Med. Surg. Public Health"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e30600","DOI":"10.1016\/j.heliyon.2024.e30600","article-title":"A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic","volume":"10","author":"Rashid","year":"2024","journal-title":"Heliyon"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"100939","DOI":"10.1016\/j.lanmic.2024.07.002","article-title":"Global wastewater surveillance for pathogens with pandemic potential: Opportunities and challenges","volume":"6","author":"Grassly","year":"2025","journal-title":"Lancet Microbe"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"e976","DOI":"10.1016\/S2214-109X(23)00170-5","article-title":"Wastewater monitoring can anchor global disease surveillance systems","volume":"11","author":"Keshaviah","year":"2023","journal-title":"Lancet Glob. Health"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"158964","DOI":"10.1016\/j.scitotenv.2022.158964","article-title":"Does normalization of SARS-CoV-2 concentrations by Pepper Mild Mottle Virus improve correlations and lead time between wastewater surveillance and clinical data in Alberta (Canada): Comparing twelve SARS-CoV-2 normalization approaches","volume":"856","author":"Qiu","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_80","unstructured":"Rogers, E. (2025). Variability and Uncertainty in SARS-CoV-2 Wastewater-Based Surveillance Normalization: A Systematic Review. [Master's Thesis, University of Washington]."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"152597","DOI":"10.1016\/j.scitotenv.2021.152597","article-title":"Association of SARS-CoV-2 presence in sewage with public adherence to precautionary measures and reported COVID-19 prevalence in Tehran","volume":"812","author":"Amereh","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"3514","DOI":"10.1021\/acs.est.0c08210","article-title":"Challenges in Measuring the Recovery of SARS-CoV-2 from Wastewater","volume":"55","author":"Kantor","year":"2021","journal-title":"Environ. Sci. Technol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"02011","DOI":"10.1051\/e3sconf\/202343702011","article-title":"Evaluating Recovery Control Concentrations of Bovine Coronavirus (EVAg 015V-02282) Used for SARS-CoV-2 Wastewater Surveillance","volume":"437","author":"Prangate","year":"2023","journal-title":"E3S Web. Conf."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"178101","DOI":"10.1016\/j.scitotenv.2024.178101","article-title":"Abundance and possibilities of crAssphage and PMMoV as a viral indicator in raw sewage in wastewater treatment plants","volume":"963","author":"Djoulissa","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Benefield, A.E., Skrip, L.A., Clement, A., Althouse, R.A., Chang, S., and Althouse, B.M. (2020). SARS-CoV-2 viral load peaks prior to symptom onset: A systematic review and individual-pooled analysis of coronavirus viral load from 66 studies. medRxiv.","DOI":"10.1101\/2020.09.28.20202028"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"McSparron, C., Bell, S.H., Nejad, B.F., Allen, D.M., Reyne, M.I., Lee, A., Lock, J., Levickas, A., Fitzgerald, A., and Creevey, C. (2023). Wastewater Flow Estimation, Using Localised Rainfall Data, for SARS-CoV-2 Monitoring Via an Optimised High-Throughput Surveillance Platform.","DOI":"10.2139\/ssrn.4442604"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"161196","DOI":"10.1016\/j.scitotenv.2022.161196","article-title":"Normalisation of SARS-CoV-2 concentrations in wastewater: The use of flow, electrical conductivity and crAssphage","volume":"865","author":"Langeveld","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"118827","DOI":"10.1016\/j.watres.2022.118827","article-title":"CrAssphage as an indicator of human-fecal contamination in water environment and virus reduction in wastewater treatment","volume":"221","author":"Sabar","year":"2022","journal-title":"Water Res."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"100363","DOI":"10.1016\/j.coesh.2022.100363","article-title":"Sewage surveillance for SARS-CoV-2: Molecular detection, quantification, and normalization factors","volume":"28","author":"Mazumder","year":"2022","journal-title":"Curr. Opin. Environ. Sci. Health"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"116560","DOI":"10.1016\/j.watres.2020.116560","article-title":"Quantitative analysis of SARS-CoV-2 RNA from wastewater solids in communities with low COVID-19 incidence and prevalence","volume":"188","author":"Mercier","year":"2021","journal-title":"Water Res."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Rainey, A.L., Liang, S., Bisesi, J.H., Sabo-Attwood, T., and Maurelli, A.T. (2023). A multistate assessment of population normalization factors for wastewater-based epidemiology of COVID-19. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0284370"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"118985","DOI":"10.1016\/j.watres.2022.118985","article-title":"Biomarkers selection for population normalization in SARS-CoV-2 wastewater-based epidemiology","volume":"223","author":"Hsu","year":"2022","journal-title":"Water Res."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"103720","DOI":"10.1016\/j.eti.2024.103720","article-title":"Multi-factor normalisation of viral counts from wastewater improves the detection accuracy of viral disease in the community","volume":"36","author":"Pellett","year":"2024","journal-title":"Environ. Technol. Innov."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Verani, M., Federigi, I., Angori, A., Pagani, A., Marvulli, F., Valentini, C., Atomsa, N.T., Conte, B., and Carducci, A. (2025). Evaluating Population Normalization Methods Using Chemical Data for Wastewater-Based Epidemiology: Insights from a Site-Specific Case Study. Viruses, 17.","DOI":"10.3390\/v17050672"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"775","DOI":"10.2166\/wh.2021.133","article-title":"Detection of SARS-CoV-2 in wastewater is influenced by sampling time, concentration method, and target analyzed","volume":"19","author":"Pino","year":"2021","journal-title":"J. Water Health"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"144105","DOI":"10.1016\/j.scitotenv.2020.144105","article-title":"Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters","volume":"756","author":"Barril","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s12560-021-09488-8","article-title":"Methods Evaluation for Rapid Concentration and Quantification of SARS-CoV-2 in Raw Wastewater Using Droplet Digital and Quantitative RT-PCR","volume":"13","author":"Flood","year":"2021","journal-title":"Food Environ. Virol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"143870","DOI":"10.1016\/j.scitotenv.2020.143870","article-title":"Comparing analytical methods to detect SARS-CoV-2 in wastewater","volume":"758","author":"Randazzo","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"e53175","DOI":"10.2196\/53175","article-title":"Comparison of Different Reverse Transcriptase\u2013Polymerase Chain Reaction\u2013Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study","volume":"10","author":"Lehto","year":"2024","journal-title":"JMIR Public Health Surveill."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1007\/s12560-022-09522-3","article-title":"Integration of RT-LAMP and Microfluidic Technology for Detection of SARS-CoV-2 in Wastewater as an Advanced Point-of-Care Platform","volume":"14","author":"Donia","year":"2022","journal-title":"Food Environ. Virol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"e202404698","DOI":"10.1002\/slct.202404698","article-title":"Recent Technologies for the Determination of SARS-CoV-2 in Wastewater","volume":"10","author":"Gazu","year":"2025","journal-title":"ChemistrySelect"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-025-03927-6","article-title":"SARS-CoV-2 wastewater genomic surveillance: Approaches, challenges, and opportunities","volume":"27","author":"Munteanu","year":"2026","journal-title":"Genome Biol."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Gordeev, V., H\u00f6lzer, M., Desir\u00f2, D., Goraichuk, I.V., Knyazev, S., Solo-Gabriele, H., Skums, P., Karthikeyan, S., Evans, A., and Agrawal, S. (2025). Leveraging wastewater sequencing to strengthen global public health surveillance. BMC Glob. Public Health, 3.","DOI":"10.1186\/s44263-025-00138-w"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.3201\/eid2610.201800","article-title":"Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2","volume":"26","author":"Paden","year":"2020","journal-title":"Emerg. Infect. Dis. J."},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Arana, C., Liang, C., Brock, M., Zhang, B., Zhou, J., Chen, L., Cantarel, B., SoRelle, J., Hooper, L.V., and Raj, P. (2022). A short plus long-amplicon based sequencing approach improves genomic coverage and variant detection in the SARS-CoV-2 genome. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0261014"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/s41586-022-05049-6","article-title":"Wastewater sequencing reveals early cryptic SARS-CoV-2 variant transmission","volume":"609","author":"Karthikeyan","year":"2022","journal-title":"Nature"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Aberi, P., Arabzadeh, R., Insam, H., Markt, R., Mayr, M., Kreuzinger, N., and Rauch, W. (2021). Quest for Optimal Regression Models in SARS-CoV-2 Wastewater Based Epidemiology. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph182010778"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"118611","DOI":"10.1016\/j.watres.2022.118611","article-title":"Longitudinal SARS-CoV-2 RNA wastewater monitoring across a range of scales correlates with total and regional COVID-19 burden in a well-defined urban population","volume":"220","author":"Acosta","year":"2022","journal-title":"Water Res."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"117252","DOI":"10.1016\/j.watres.2021.117252","article-title":"Wastewater monitoring outperforms case numbers as a tool to track COVID-19 incidence dynamics when test positivity rates are high","volume":"200","author":"Scheidegger","year":"2021","journal-title":"Water Res."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"163049","DOI":"10.1016\/j.scitotenv.2023.163049","article-title":"Survival of SARS-CoV-2 in wastewater","volume":"882","author":"Sherchan","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"100347","DOI":"10.1016\/j.envadv.2023.100347","article-title":"SARS-CoV-2 concentration in wastewater consistently predicts trends in COVID-19 case counts by at least two days across multiple WWTP scales","volume":"11","author":"Swift","year":"2023","journal-title":"Environ. Adv."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1093\/infdis\/jiac089","article-title":"Paradoxically Greater Persistence of HIV RNA-Positive Cells in Lymphoid Tissue When ART Is Initiated in the Earliest Stage of Infection","volume":"225","author":"Kroon","year":"2022","journal-title":"J. Infect. Dis."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"e68","DOI":"10.1017\/S0950268825100058","article-title":"Application of joinpoint regression to SARS-CoV-2 wastewater-based epidemiology in Las Vegas, Nevada, USA","volume":"153","author":"Barber","year":"2025","journal-title":"Epidemiol. Infect."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"155828","DOI":"10.1016\/j.scitotenv.2022.155828","article-title":"SARS-CoV-2 variant trends in Ireland: Wastewater-based epidemiology and clinical surveillance","volume":"838","author":"Reynolds","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"151169","DOI":"10.1016\/j.scitotenv.2021.151169","article-title":"SARS-CoV-2 RNA surveillance in large to small centralized wastewater treatment plants preceding the third COVID-19 resurgence in Bangkok, Thailand","volume":"809","author":"Sangsanont","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"152213","DOI":"10.1016\/j.scitotenv.2021.152213","article-title":"SARS-CoV-2 genome quantification in wastewaters at regional and city scale allows precise monitoring of the whole outbreaks dynamics and variants spreading in the population","volume":"810","author":"Wurtzer","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"114","DOI":"10.2166\/wh.2021.180","article-title":"Detection and abundance of SARS-CoV-2 in wastewater in Liechtenstein, and the estimation of prevalence and impact of the B.1.1.7 variant","volume":"20","author":"Markt","year":"2022","journal-title":"J. Water Health"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"166446","DOI":"10.1016\/j.scitotenv.2023.166446","article-title":"Positive association of SARS-CoV-2 RNA concentrations in wastewater and reported COVID-19 cases in Singapore\u2014A study across three populations","volume":"902","author":"Wong","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"149757","DOI":"10.1016\/j.scitotenv.2021.149757","article-title":"Wastewater SARS-CoV-2 monitoring as a community-level COVID-19 trend tracker and variants in Ohio, United States","volume":"801","author":"Ai","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"150573","DOI":"10.1016\/j.scitotenv.2021.150573","article-title":"Associations between SARS-CoV-2 RNA concentrations in wastewater and COVID-19 rates in days after sampling in small urban areas of Seville: A time series study","volume":"806","author":"Lorusso","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"118070","DOI":"10.1016\/j.watres.2022.118070","article-title":"Metrics to relate COVID-19 wastewater data to clinical testing dynamics","volume":"212","author":"Xiao","year":"2022","journal-title":"Water Res."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"118481","DOI":"10.1016\/j.watres.2022.118481","article-title":"Monitoring of SARS-CoV-2 in sewersheds with low COVID-19 cases using a passive sampling technique","volume":"218","author":"Li","year":"2022","journal-title":"Water Res."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"w30202","DOI":"10.4414\/SMW.2022.w30202","article-title":"Wastewater monitoring of SARS-CoV-2 shows high correlation with COVID-19 case numbers and allowed early detection of the first confirmed B.1.1.529 infection in Switzerland: Results of an observational surveillance study","volume":"152","author":"Bagutti","year":"2022","journal-title":"Swiss Med. Wkly."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"19456","DOI":"10.1038\/s41598-021-98653-x","article-title":"Mathematical modeling based on RT-qPCR analysis of SARS-CoV-2 in wastewater as a tool for epidemiology","volume":"11","author":"Krahulec","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"144216","DOI":"10.1016\/j.scitotenv.2020.144216","article-title":"SARS-CoV-2 RNA monitoring in wastewater as a potential early warning system for COVID-19 transmission in the community: A temporal case study","volume":"761","author":"Ahmed","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"e00045-21","DOI":"10.1128\/msystems.00045-21","article-title":"High-Throughput Wastewater SARS-CoV-2 Detection Enables Forecasting of Community Infection Dynamics in San Diego County","volume":"6","author":"Karthikeyan","year":"2021","journal-title":"mSystems"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"116620","DOI":"10.1016\/j.watres.2020.116620","article-title":"Surveillance of wastewater revealed peaks of SARS-CoV-2 preceding those of hospitalized patients with COVID-19","volume":"189","author":"Saguti","year":"2021","journal-title":"Water Res."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"169028","DOI":"10.1016\/j.scitotenv.2023.169028","article-title":"Beyond linear regression: Modeling COVID-19 clinical cases with wastewater surveillance of SARS-CoV-2 for the city of Athens and Ohio University campus","volume":"912","author":"Rezaeitavabe","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"122471","DOI":"10.1016\/j.envpol.2023.122471","article-title":"Response of wastewater-based epidemiology predictor for the second wave of COVID-19 in Ahmedabad, India: A long-term data Perspective","volume":"337","author":"Kumar","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"150151","DOI":"10.1016\/j.scitotenv.2021.150151","article-title":"SARS-CoV-2 wastewater surveillance data can predict hospitalizations and ICU admissions","volume":"804","author":"Galani","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"148442","DOI":"10.1016\/j.scitotenv.2021.148442","article-title":"Age-dependent association between SARS-CoV-2 cases reported by passive surveillance and viral load in wastewater","volume":"792","author":"Omori","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s40246-024-00641-5","article-title":"EU surveys insights: Analytical tools, future directions, and the essential requirement for reference materials in wastewater monitoring of SARS-CoV-2, antimicrobial resistance and beyond","volume":"18","author":"Paracchini","year":"2024","journal-title":"Hum. Genom."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"171566","DOI":"10.1016\/j.scitotenv.2024.171566","article-title":"The National Wastewater Surveillance System (NWSS): From inception to widespread coverage, 2020\u20132022, United States","volume":"924","author":"Adams","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_134","unstructured":"WHO (2025, February 25). COVID-19 Wastewater. WHO COVID-19 Dashboard. Available online: https:\/\/data.who.int\/dashboards\/covid19\/wastewater."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1007\/s11116-023-10392-2","article-title":"Systematic literature review on impacts of COVID-19 pandemic and corresponding measures on mobility","volume":"51","author":"Lee","year":"2024","journal-title":"Transportation"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"e70020","DOI":"10.1111\/1751-7915.70020","article-title":"Tracking epidemic viruses in wastewaters","volume":"17","year":"2024","journal-title":"Microb. Biotechnol."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"e202400012","DOI":"10.1002\/appl.202400012","article-title":"Leveraging wastewater surveillance to actively monitor Covid-19 community dynamics in rural areas with reduced reliance on clinical testing","volume":"3","author":"Jarvie","year":"2024","journal-title":"Appl. Res."},{"key":"ref_138","first-page":"1545","article-title":"Standardizing data reporting in the research community to enhance the utility of open data for SARS-CoV-2 wastewater surveillance","volume":"9","author":"Aanderud","year":"2021","journal-title":"Environ. Sci. Water Res. Technol."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"100429","DOI":"10.1016\/j.ijregi.2024.100429","article-title":"Inter-institutional laboratory standardization for SARS-CoV-2 surveillance through wastewater-based epidemiology applied to Mexico City","volume":"12","author":"Campos","year":"2024","journal-title":"IJID Reg."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"e33873","DOI":"10.1016\/j.heliyon.2024.e33873","article-title":"Wastewater surveillance for viral pathogens: A tool for public health","volume":"10","author":"Silva","year":"2024","journal-title":"Heliyon"},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"179706","DOI":"10.1016\/j.scitotenv.2025.179706","article-title":"Wastewater-based epidemiology of influenza viruses: A systematic review","volume":"986","author":"Viviani","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"28194","DOI":"10.1038\/s41598-025-13998-x","article-title":"Methodic aspects of influenza and respiratory syncytial virus detection in raw wastewater and presence in treatment plants in southeastern Germany","volume":"15","author":"Geissler","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"121650","DOI":"10.1016\/j.watres.2024.121650","article-title":"Wastewater-based surveillance is an efficient monitoring tool for tracking influenza A in the community","volume":"257","author":"Lehto","year":"2024","journal-title":"Water Res."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"122152","DOI":"10.1016\/j.watres.2024.122152","article-title":"Piloting wastewater-based surveillance of norovirus in England","volume":"263","author":"Walker","year":"2024","journal-title":"Water Res."},{"key":"ref_145","doi-asserted-by":"crossref","unstructured":"Bonanno Ferraro, G., Brandtner, D., Mancini, P., Veneri, C., Iaconelli, M., Suffredini, E., and La Rosa, G. (2025). Eight Years of Norovirus Surveillance in Urban Wastewater: Insights from Next-Generation. Viruses, 17.","DOI":"10.3390\/v17010130"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s12560-023-09573-0","article-title":"Critical Needs for Integrated Surveillance: Wastewater-Based and Clinical Epidemiology in Evolving Scenarios with Lessons Learned from SARS-CoV-2","volume":"16","author":"Carducci","year":"2024","journal-title":"Food Environ. Virol."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1039\/D4EW00654B","article-title":"Integrating wastewater analysis and targeted clinical testing for early disease outbreak detection and an enhanced public health response","volume":"11","author":"Contreras","year":"2025","journal-title":"Environ. Sci. Water Res. Technol."},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"100619","DOI":"10.1016\/j.lansea.2025.100619","article-title":"Identifying bellwether sewershed sites for sustainable disease surveillance in Bengaluru, India: A longitudinal study","volume":"39","author":"Daniel","year":"2025","journal-title":"Lancet Reg. Health\u2014Southeast Asia"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1186\/s12985-025-02737-3","article-title":"Immune evasion, infectivity, and membrane fusion of the SARS-CoV-2 JN.1 variant","volume":"22","author":"Tang","year":"2025","journal-title":"Virol. J."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"100825","DOI":"10.1016\/j.epidem.2025.100825","article-title":"Analysis insights to support the use of wastewater and environmental surveillance data for infectious diseases and pandemic preparedness","volume":"51","author":"Wade","year":"2025","journal-title":"Epidemics"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"139652","DOI":"10.1016\/j.scitotenv.2020.139652","article-title":"First detection of SARS-CoV-2 in untreated wastewaters in Italy","volume":"736","author":"Iaconelli","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"143329","DOI":"10.1016\/j.scitotenv.2020.143329","article-title":"SARS-CoV-2 RNA detection and persistence in wastewater samples: An experimental network for COVID-19 environmental surveillance in Padua, Veneto Region (NE Italy)","volume":"760","author":"Baldovin","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s12560-023-09565-0","article-title":"Wastewater-based Epidemiology and SARS-CoV-2: Variant Trends in the Apulia Region (Southern Italy) and Effect of Some Environmental Parameters","volume":"15","author":"Triggiano","year":"2023","journal-title":"Food Environ. Virol."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"150816","DOI":"10.1016\/j.scitotenv.2021.150816","article-title":"SARS-CoV-2 RNA in urban wastewater samples to monitor the COVID-19 pandemic in Lombardy, Italy (March\u2013June 2020)","volume":"806","author":"Castiglioni","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_155","doi-asserted-by":"crossref","unstructured":"Giglio, O.D., Triggiano, F., Apollonio, F., Diella, G., Fasano, F., Stefanizzi, P., Lopuzzo, M., Brigida, S., Calia, C., and Pousis, C. (2021). Potential Use of Untreated Wastewater for Assessing COVID-19 Trends in Southern Italy. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph181910278"},{"key":"ref_156","doi-asserted-by":"crossref","unstructured":"Verani, M., Pagani, A., Federigi, I., Lauretani, G., Atomsa, N.T., Rossi, V., Viviani, L., and Carducci, A. (2024). Wastewater-Based Epidemiology for Viral Surveillance from an Endemic Perspective: Evidence and Challenges. Viruses, 16.","DOI":"10.20944\/preprints202402.0463.v1"},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"114956","DOI":"10.1016\/j.jviromet.2024.114956","article-title":"Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples","volume":"328","author":"Morecchiato","year":"2024","journal-title":"J. Virol. Methods."},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1021\/acsestwater.2c00013","article-title":"Molecular Monitoring of SARS-CoV-2 in Different Sewage Plants in Venice and the Implications for Genetic Surveillance","volume":"2","author":"Brian","year":"2022","journal-title":"ACS EST Water"},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"116810","DOI":"10.1016\/j.watres.2021.116810","article-title":"Wastewater-based epidemiology as a useful tool to track SARS-CoV-2 and support public health policies at municipal level in Brazil","volume":"191","author":"Prado","year":"2021","journal-title":"Water Res."},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"153927","DOI":"10.1016\/j.scitotenv.2022.153927","article-title":"First case of SARS-CoV-2 RNA detection in municipal solid waste leachate from Brazil","volume":"824","author":"Mondelli","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_161","first-page":"100008","article-title":"Wastewater-based epidemiological investigation of SARS-CoV-2 in Porto Alegre, Southern Brazil","volume":"1","author":"Mangini","year":"2023","journal-title":"Sci. One Health"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"268","DOI":"10.2166\/wh.2024.159","article-title":"COVID-19 cases, vaccination, and SARS-CoV-2 in wastewater: Insights from a Brazilian municipality","volume":"22","author":"Lopes","year":"2024","journal-title":"J. Water Health."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1007\/s11356-023-31081-8","article-title":"Environmental monitoring of SARS-CoV-2 in the metropolitan area of Porto Alegre, Rio Grande do Sul (RS), Brazil","volume":"31","author":"Dutra","year":"2024","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"108298","DOI":"10.1016\/j.jece.2022.108298","article-title":"Wastewater-based epidemiology: A Brazilian SARS-COV-2 surveillance experience","volume":"10","author":"Claro","year":"2022","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"107478","DOI":"10.1016\/j.jece.2022.107478","article-title":"Sampling strategies for wastewater surveillance: Evaluating the variability of SARS-COV-2 RNA concentration in composite and grab samples","volume":"10","author":"Augusto","year":"2022","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"nwad089","DOI":"10.1093\/nsr\/nwad089","article-title":"Wastewater surveillance and an automated robot: Effectively tracking SARS-CoV-2 transmission in the post-epidemic era","volume":"10","author":"Ou","year":"2023","journal-title":"Natl. Sci. Rev."},{"key":"ref_167","doi-asserted-by":"crossref","first-page":"e35332","DOI":"10.1016\/j.heliyon.2024.e35332","article-title":"Wastewater multiplex PCR amplicon sequencing revealed community transmission of SARS-CoV-2 lineages during the outbreak of infection in Chinese Mainland","volume":"10","author":"Tang","year":"2024","journal-title":"Heliyon"},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"148000","DOI":"10.1016\/j.scitotenv.2021.148000","article-title":"The first case study of wastewater-based epidemiology of COVID-19 in Hong Kong","volume":"790","author":"Xu","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_169","doi-asserted-by":"crossref","unstructured":"Reynolds, L.J., Sala-Comorera, L., Khan, M.F., Martin, N.A., Whitty, M., Stephens, J.H., Nolan, T.M., Joyce, E., Fletcher, N.F., and Murphy, C.D. (2022). Coprostanol as a Population Biomarker for SARS-CoV-2 Wastewater Surveillance Studies. Water, 14.","DOI":"10.3390\/w14020225"},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1017\/S0950268824000190","article-title":"Near-source passive sampling for monitoring viral outbreaks within a university residential setting","volume":"152","author":"Farkas","year":"2024","journal-title":"Epidemiol. Infect."},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/s41597-022-01788-3","article-title":"SARS-CoV-2 RNA levels in Scotland\u2019s wastewater","volume":"9","author":"Scorza","year":"2022","journal-title":"Sci. Data"},{"key":"ref_172","doi-asserted-by":"crossref","first-page":"113692","DOI":"10.1016\/j.ijheh.2021.113692","article-title":"Epidemiological surveillance of SARS-CoV-2 by genome quantification in wastewater applied to a city in the northeast of France: Comparison of ultrafiltration- and protein precipitation-based methods","volume":"233","author":"Bertrand","year":"2021","journal-title":"Int. J. Hyg. Environ. Health."},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"67442","DOI":"10.1007\/s11356-022-22665-x","article-title":"Wild type and variants of SARS-COV-2 in Parisian sewage: Presence in raw water and through processes in wastewater treatment plants","volume":"29","author":"Viveros","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"2000776","DOI":"10.2807\/1560-7917.ES.2020.25.50.2000776","article-title":"Evaluation of lockdown effect on SARS-CoV-2 dynamics through viral genome quantification in waste water, Greater Paris, France, 5 March to 23 April 2020","volume":"25","author":"Wurtzer","year":"2020","journal-title":"Eurosurveillance"},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"120959","DOI":"10.1016\/j.watres.2023.120959","article-title":"Highly efficient and sensitive membrane-based concentration process allows quantification, surveillance, and sequencing of viruses in large volumes of wastewater","volume":"249","author":"Gerber","year":"2024","journal-title":"Water Res."},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"175823","DOI":"10.1016\/j.scitotenv.2024.175823","article-title":"Definition of a concentration and RNA extraction protocol for optimal whole genome sequencing of SARS-CoV-2 in wastewater (ANRS0160)","volume":"952","author":"Chaqroun","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_177","doi-asserted-by":"crossref","first-page":"118257","DOI":"10.1016\/j.watres.2022.118257","article-title":"Emergence of SARS-CoV-2 Alpha lineage and its correlation with quantitative wastewater-based epidemiology data","volume":"215","author":"Radu","year":"2022","journal-title":"Water Res."},{"key":"ref_178","first-page":"620","article-title":"Detection of SARS-CoV-2 RNA with a Simple Concentration Method in Wastewater in Turkey: A Pilot Study in \u00c7orum","volume":"26","author":"Karasartova","year":"2021","journal-title":"Flora J. Infect. Dis. Clin. Microbiol."},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"150244","DOI":"10.1016\/j.scitotenv.2021.150244","article-title":"Monitoring SARS-CoV-2 in sewage: Toward sentinels with analytical accuracy","volume":"804","author":"Orschler","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"106938","DOI":"10.1016\/j.envint.2021.106938","article-title":"Wastewater surveillance demonstrates high predictive value for COVID-19 infection on board repatriation flights to Australia","volume":"158","author":"Ahmed","year":"2022","journal-title":"Environ. Int."},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"150572","DOI":"10.1016\/j.scitotenv.2021.150572","article-title":"Analytical performance comparison of four SARS-CoV-2 RT-qPCR primer-probe sets for wastewater samples","volume":"806","author":"Zhang","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_182","first-page":"100086","article-title":"Early-pandemic wastewater surveillance of SARS-CoV-2 in Southern Nevada: Methodology, occurrence, and incidence\/prevalence considerations","volume":"10","author":"Gerrity","year":"2021","journal-title":"Water Res. X"},{"key":"ref_183","doi-asserted-by":"crossref","unstructured":"Li, Y., Ash, K.T., Joyner, D.C., Williams, D.E., Alamilla, I., McKay, P.J., Iler, C., and Hazen, T.C. (2023). Evaluating various composite sampling modes for detecting pathogenic SARS-CoV-2 virus in raw sewage. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1305967"},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"149177","DOI":"10.1016\/j.scitotenv.2021.149177","article-title":"Lessons learned from SARS-CoV-2 measurements in wastewater","volume":"798","author":"Sharkey","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"140621","DOI":"10.1016\/j.scitotenv.2020.140621","article-title":"First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA","volume":"743","author":"Sherchan","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_186","doi-asserted-by":"crossref","first-page":"149930","DOI":"10.1016\/j.scitotenv.2021.149930","article-title":"SARS-CoV-2 variant detection at a university dormitory using wastewater genomic tools","volume":"805","author":"Vo","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"16141","DOI":"10.1038\/s41598-022-20219-2","article-title":"Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities","volume":"12","author":"Li","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_188","doi-asserted-by":"crossref","first-page":"e15079","DOI":"10.7717\/peerj.15079","article-title":"Wastewater monitoring of SARS-CoV-2 RNA at K-12 schools: Comparison to pooled clinical testing data","volume":"11","author":"Kim","year":"2023","journal-title":"PeerJ"},{"key":"ref_189","first-page":"88","article-title":"Solid\u2013liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA","volume":"11","author":"Oost","year":"2024","journal-title":"Environ. Sci. Water Res. Technol."},{"key":"ref_190","doi-asserted-by":"crossref","first-page":"117093","DOI":"10.1016\/j.watres.2021.117093","article-title":"Fine-Scale Temporal Dynamics of SARS-CoV-2 RNA Abundance in Wastewater during A COVID-19 Lockdown","volume":"197","author":"Li","year":"2021","journal-title":"Water Res."},{"key":"ref_191","doi-asserted-by":"crossref","first-page":"117400","DOI":"10.1016\/j.watres.2021.117400","article-title":"Wastewater surveillance of SARS-CoV-2 across 40 U.S. States from February to June 2020","volume":"202","author":"Wu","year":"2021","journal-title":"Water Res."},{"key":"ref_192","doi-asserted-by":"crossref","first-page":"e00614-20","DOI":"10.1128\/mSystems.00614-20","article-title":"SARS-CoV-2 titers in wastewater are higher than expected from clinically confirmed cases","volume":"5","author":"Wu","year":"2020","journal-title":"mSystems"},{"key":"ref_193","doi-asserted-by":"crossref","first-page":"119648","DOI":"10.1016\/j.watres.2023.119648","article-title":"Wastewater surveillance of SARS-CoV-2 and influenza in preK-12 schools shows school, community, and citywide infections","volume":"231","author":"Wolken","year":"2023","journal-title":"Water Res."},{"key":"ref_194","doi-asserted-by":"crossref","first-page":"114277","DOI":"10.1016\/j.envres.2022.114277","article-title":"Building-level wastewater surveillance of SARS-CoV-2 is associated with transmission and variant trends in a university setting","volume":"215","author":"Sellers","year":"2022","journal-title":"Environ. Res."},{"key":"ref_195","doi-asserted-by":"crossref","first-page":"159996","DOI":"10.1016\/j.scitotenv.2022.159996","article-title":"Detection of SARS-CoV-2 RNA in wastewater and comparison to COVID-19 cases in two sewersheds, North Carolina, USA","volume":"858","author":"Grube","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_196","doi-asserted-by":"crossref","first-page":"151534","DOI":"10.1016\/j.scitotenv.2021.151534","article-title":"SARS-CoV-2 monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data","volume":"816","author":"Nagarkar","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_197","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1021\/acsestwater.2c00275","article-title":"Retrospective Analysis of Wastewater-Based Epidemiology of SARS-CoV-2 in Residences on a Large College Campus: Relationships between Wastewater Outcomes and COVID-19 Cases across Two Semesters with Different COVID-19 Mitigation Policies","volume":"3","author":"Rainey","year":"2023","journal-title":"ACS EST Water"},{"key":"ref_198","doi-asserted-by":"crossref","first-page":"155410","DOI":"10.1016\/j.scitotenv.2022.155410","article-title":"Use of wastewater surveillance for early detection of Alpha and Epsilon SARS-CoV-2 variants of concern and estimation of overall COVID-19 infection burden","volume":"835","author":"Vo","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_199","doi-asserted-by":"crossref","first-page":"111374","DOI":"10.1016\/j.envres.2021.111374","article-title":"Targeted wastewater surveillance of SARS-CoV-2 on a university campus for COVID-19 outbreak detection and mitigation","volume":"200","author":"Scott","year":"2021","journal-title":"Environ. Res."},{"key":"ref_200","doi-asserted-by":"crossref","unstructured":"Innes, G.K., Schmitz, B.W., Brierley, P.E., Guzman, J., Prasek, S.M., Ruedas, M., Sanchez, A., Bhattacharjee, S., and Slinski, S. (2022). Wastewater-Based Epidemiology Mitigates COVID-19 Outbreaks at a Food Processing Facility near the Mexico\u2013U.S. Border\u2014November 2020\u2013March 2022. Viruses, 14.","DOI":"10.3390\/v14122684"},{"key":"ref_201","doi-asserted-by":"crossref","first-page":"158350","DOI":"10.1016\/j.scitotenv.2022.158350","article-title":"Surveillance of SARS-CoV-2 in nine neighborhood sewersheds in Detroit Tri-County area, United States: Assessing per capita SARS-CoV-2 estimations and COVID-19 incidence","volume":"851","author":"Li","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_202","doi-asserted-by":"crossref","unstructured":"Zhao, L., Geng, Q., Corchis-Scott, R., McKay, R.M., Norton, J., and Xagoraraki, I. (2023). Targeting a free viral fraction enhances the early alert potential of wastewater surveillance for SARS-CoV-2: A methods comparison spanning the transition between delta and omicron variants in a large urban center. Front. Public Health., 11.","DOI":"10.3389\/fpubh.2023.1140441"},{"key":"ref_203","doi-asserted-by":"crossref","first-page":"152503","DOI":"10.1016\/j.scitotenv.2021.152503","article-title":"Tracking the temporal variation of COVID-19 surges through wastewater-based epidemiology during the peak of the pandemic: A six-month long study in Charlotte, North Carolina","volume":"814","author":"Barua","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_204","doi-asserted-by":"crossref","first-page":"155401","DOI":"10.1016\/j.scitotenv.2022.155401","article-title":"Predictive values of time-dense SARS-CoV-2 wastewater analysis in university campus buildings","volume":"835","author":"Welling","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_205","doi-asserted-by":"crossref","unstructured":"Godinez, A., Hill, D., Dandaraw, B., Green, H., Kilaru, P., Middleton, F., Run, S., Kmush, B.L., and Larsen, D.A. (2022). High Sensitivity and Specificity of Dormitory-Level Wastewater Surveillance for COVID-19 during Fall Semester 2020 at Syracuse University, New York. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19084851"},{"key":"ref_206","doi-asserted-by":"crossref","unstructured":"Acer, P.T., Kelly, L.M., Lover, A.A., and Butler, C.S. (2022). Quantifying the Relationship between SARS-CoV-2 Wastewater Concentrations and Building-Level COVID-19 Prevalence at an Isolation Residence: A Passive Sampling Approach. Int. J. Environ. Res. Public Health, 19.","DOI":"10.1101\/2022.04.07.22273534"},{"key":"ref_207","doi-asserted-by":"crossref","first-page":"148834","DOI":"10.1016\/j.scitotenv.2021.148834","article-title":"A direct capture method for purification and detection of viral nucleic acid enables epidemiological surveillance of SARS-CoV-2","volume":"795","author":"Mondal","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_208","doi-asserted-by":"crossref","first-page":"150390","DOI":"10.1016\/j.scitotenv.2021.150390","article-title":"Spatial and temporal variability and data bias in wastewater surveillance of SARS-CoV-2 in a sewer system","volume":"805","author":"Haak","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_209","doi-asserted-by":"crossref","unstructured":"Thakali, O., Shahin, S., and Sherchan, S.P. (2024). Wastewater Surveillance of SARS-CoV-2 RNA in a Prison Facility. Water, 16.","DOI":"10.3390\/w16040570"},{"key":"ref_210","doi-asserted-by":"crossref","first-page":"xtab015","DOI":"10.1093\/femsmc\/xtab015","article-title":"Wastewater surveillance of SARS-CoV-2 corroborates heightened community infection during the initial peak of COVID-19 in Bexar County, Texas","volume":"2","author":"Moghadam","year":"2021","journal-title":"FEMS Microbes"},{"key":"ref_211","doi-asserted-by":"crossref","first-page":"149595","DOI":"10.1016\/j.scitotenv.2021.149595","article-title":"A comparison of four commercially available RNA extraction kits for wastewater surveillance of SARS-CoV-2 in a college population","volume":"801","author":"Rundell","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_212","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1039\/D3VA00015J","article-title":"Wastewater-based surveillance of COVID-19 and removal of SARS-CoV-2 RNA across a major wastewater treatment plant in San Antonio, Texas","volume":"2","author":"Phan","year":"2023","journal-title":"Environ. Sci. Adv."},{"key":"ref_213","doi-asserted-by":"crossref","first-page":"157546","DOI":"10.1016\/j.scitotenv.2022.157546","article-title":"Temporal dynamics of SARS-CoV-2 genome and detection of variants of concern in wastewater influent from two metropolitan areas in Arkansas","volume":"849","author":"Silva","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_214","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1039\/D3EW90014B","article-title":"Evaluation of intra- and inter-lab variability in quantifying SARS-CoV-2 in a state-wide wastewater monitoring network","volume":"9","author":"Davis","year":"2023","journal-title":"Environ. Sci. Water Res. Technol."},{"key":"ref_215","doi-asserted-by":"crossref","first-page":"121338","DOI":"10.1016\/j.watres.2024.121338","article-title":"Long-term monitoring of SARS-CoV-2 variants in wastewater using a coordinated workflow of droplet digital PCR and nanopore sequencing","volume":"254","author":"Vigil","year":"2024","journal-title":"Water Res."},{"key":"ref_216","doi-asserted-by":"crossref","first-page":"978","DOI":"10.2166\/wh.2024.303","article-title":"Monitoring SARS-CoV-2 RNA in wastewater from a shared septic system and sub-sewershed sites to expand COVID-19 disease surveillance","volume":"22","author":"Pasha","year":"2024","journal-title":"J. Water Health."},{"key":"ref_217","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1021\/acs.estlett.3c00938","article-title":"Environmental Surveillance of Flood Control Infrastructure Impacted by Unsheltered Individuals Leads to the Detection of SARS-CoV-2 and Novel Mutations in the Spike Gene","volume":"11","author":"Harrington","year":"2024","journal-title":"Environ. Sci. Technol. Lett."},{"key":"ref_218","doi-asserted-by":"crossref","unstructured":"Li, Y., Ash, K., Alamilla, I., Joyner, D., Williams, D.E., McKay, P.J., Green, B., DeBlander, S., North, C., and Kara-Murdoch, F. (2024). COVID-19 trends at the University of Tennessee: Predictive insights from raw sewage SARS-CoV-2 detection and evaluation and PMMoV as an indicator for human waste. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1379194"},{"key":"ref_219","doi-asserted-by":"crossref","first-page":"e01122-23","DOI":"10.1128\/spectrum.01122-23","article-title":"Analytical validation of a semi-automated methodology for quantitative measurement of SARS-CoV-2 RNA in wastewater collected in northern New England","volume":"12","author":"Robbins","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"ref_220","doi-asserted-by":"crossref","first-page":"e00829-21","DOI":"10.1128\/msystems.00829-21","article-title":"High-Frequency, High-Throughput Quantification of SARS-CoV-2 RNA in Wastewater Settled Solids at Eight Publicly Owned Treatment Works in Northern California Shows Strong Association with COVID-19 Incidence","volume":"6","author":"Wolfe","year":"2021","journal-title":"mSystems"},{"key":"ref_221","doi-asserted-by":"crossref","first-page":"115351","DOI":"10.1016\/j.envres.2023.115351","article-title":"Highly socially vulnerable communities exhibit disproportionately increased viral loads as measured in community wastewater","volume":"222","author":"Baldwin","year":"2023","journal-title":"Environ. Res."},{"key":"ref_222","doi-asserted-by":"crossref","first-page":"161467","DOI":"10.1016\/j.scitotenv.2023.161467","article-title":"Wastewater surveillance of SARS-CoV-2 at intra-city level demonstrated high resolution in tracking COVID-19 and calibration using chemical indicators","volume":"866","author":"Saingam","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_223","doi-asserted-by":"crossref","first-page":"161101","DOI":"10.1016\/j.scitotenv.2022.161101","article-title":"Parallel deployment of passive and composite samplers for surveillance and variant profiling of SARS-CoV-2 in sewage","volume":"866","author":"Cha","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_224","doi-asserted-by":"crossref","first-page":"e230550","DOI":"10.1001\/jamanetworkopen.2023.0550","article-title":"Detection of the Omicron BA.1 Variant of SARS-CoV-2 in Wastewater from a Las Vegas Tourist Area","volume":"6","author":"Vo","year":"2023","journal-title":"JAMA Netw. Open."},{"key":"ref_225","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1039\/D1EW00672J","article-title":"SARS-CoV-2 RNA abundance in wastewater as a function of distinct urban sewershed size","volume":"8","author":"Holm","year":"2022","journal-title":"Environ. Sci. Water Res. Technol."},{"key":"ref_226","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s12560-023-09557-0","article-title":"Detection of Enteroviruses and SARS-CoV-2 in Tunisian Wastewater","volume":"15","author":"Ibrahim","year":"2023","journal-title":"Food Environ. Virol."},{"key":"ref_227","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.2166\/wh.2024.377","article-title":"Tracking SARS-CoV-2 and its variants in wastewater in Tunisia","volume":"22","author":"Othman","year":"2024","journal-title":"J. Water Health."},{"key":"ref_228","doi-asserted-by":"crossref","first-page":"751","DOI":"10.2217\/fvl-2021-0050","article-title":"Detection of SARS-CoV-2 in the sewerage system in Tunisia: A promising tool to confront COVID-19 pandemic","volume":"16","author":"Jmii","year":"2021","journal-title":"Future Virol."},{"key":"ref_229","doi-asserted-by":"crossref","first-page":"5062","DOI":"10.1021\/acs.est.1c04705","article-title":"Detection of SARS-CoV-2 Proteins in Wastewater Samples by Mass Spectrometry","volume":"56","author":"Islam","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"ref_230","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jes.2022.10.006","article-title":"An efficient method to enhance recovery and detection of SARS-CoV-2 RNA in wastewater","volume":"130","author":"Kumblathan","year":"2023","journal-title":"J. Environ. Sci."},{"key":"ref_231","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/j.jes.2022.04.047","article-title":"Number of COVID-19 cases required in a population to detect SARS-CoV-2 RNA in wastewater in the province of Alberta, Canada: Sensitivity assessment","volume":"125","author":"Li","year":"2023","journal-title":"J. Environ. Sci."},{"key":"ref_232","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1021\/acsestwater.2c00143","article-title":"Wastewater-Based Surveillance Is an Effective Tool for Trending COVID-19 Prevalence in Communities: A Study of 10 Major Communities for 17 Months in Alberta","volume":"2","author":"Pang","year":"2022","journal-title":"ACS EST Water"},{"key":"ref_233","doi-asserted-by":"crossref","first-page":"151434","DOI":"10.1016\/j.scitotenv.2021.151434","article-title":"Validating and optimizing the method for molecular detection and quantification of SARS-CoV-2 in wastewater","volume":"812","author":"Qiu","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_234","doi-asserted-by":"crossref","first-page":"164261","DOI":"10.1016\/j.scitotenv.2023.164261","article-title":"Simultaneous detection of SARS-CoV-2, influenza A, respiratory syncytial virus, and measles in wastewater by multiplex RT-qPCR","volume":"889","author":"Hayes","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_235","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1039\/D4EW00350K","article-title":"Detection of Omicron variant in November 2021: A retrospective analysis through wastewater in Halifax, Canada","volume":"11","author":"Hayes","year":"2024","journal-title":"Environ. Sci. Water Res. Technol."},{"key":"ref_236","doi-asserted-by":"crossref","first-page":"117369","DOI":"10.1016\/j.watres.2021.117369","article-title":"A multicenter study investigating SARS-CoV-2 in tertiary-care hospital wastewater. viral burden correlates with increasing hospitalized cases as well as hospital-associated transmissions and outbreaks","volume":"201","author":"Acosta","year":"2021","journal-title":"Water Res."},{"key":"ref_237","doi-asserted-by":"crossref","first-page":"e00792-21","DOI":"10.1128\/Spectrum.00792-21","article-title":"Averting an Outbreak of SARS-CoV-2 in a University Residence Hall through Wastewater Surveillance","volume":"9","author":"Geng","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"ref_238","doi-asserted-by":"crossref","unstructured":"Avgeris, M., Adamopoulos, P.G., Galani, A., Xagorari, M., Gourgiotis, D., Trougakos, I.P., Voulgaris, N., Dimopoulos, M.-A., Thomaidis, N.S., and Scorilas, A. (2021). Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22168498"},{"key":"ref_239","doi-asserted-by":"crossref","first-page":"2659","DOI":"10.1038\/s41598-022-06625-6","article-title":"Detecting SARS-CoV-2 lineages and mutational load in municipal wastewater and a use-case in the metropolitan area of Thessaloniki, Greece","volume":"12","author":"Pechlivanis","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_240","doi-asserted-by":"crossref","first-page":"150264","DOI":"10.1016\/j.scitotenv.2021.150264","article-title":"A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal","volume":"804","author":"Monteiro","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_241","doi-asserted-by":"crossref","unstructured":"Murni, I.K., Oktaria, V., Handley, A., McCarthy, D.T., Donato, C.M., Nuryastuti, T., Supriyati, E., Putri, D.A.D., Sari, H.M., and Laksono, I.S. (2022). The feasibility of SARS-CoV-2 surveillance using wastewater and environmental sampling in Indonesia. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0274793"},{"key":"ref_242","doi-asserted-by":"crossref","unstructured":"Sanju\u00e1n, R., and Domingo-Calap, P. (2021). Reliability of Wastewater Analysis for Monitoring COVID-19 Incidence Revealed by a Long-Term Follow-Up Study. Front. Virol., 1.","DOI":"10.3389\/fviro.2021.776998"},{"key":"ref_243","doi-asserted-by":"crossref","first-page":"157370","DOI":"10.1016\/j.scitotenv.2022.157370","article-title":"Monitoring the evolution of SARS-CoV-2 on a Spanish university campus through wastewater analysis: A pilot project for the reopening strategy","volume":"845","author":"Barneo","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_244","doi-asserted-by":"crossref","first-page":"115942","DOI":"10.1016\/j.watres.2020.115942","article-title":"SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area","volume":"181","author":"Randazzo","year":"2020","journal-title":"Water Res."},{"key":"ref_245","doi-asserted-by":"crossref","unstructured":"de Alba, \u00c1.E.M., Mor\u00e1n-Diez, M.E., Garc\u00eda-Prieto, J.C., Garc\u00eda-Bernalt Diego, J., Fern\u00e1ndez-Soto, P., Serrano Le\u00f3n, E., Monsalvo, V., Casao, M., Rubio, M.B., and Hermosa, R. (2024). SARS-CoV-2 RNA Detection in Wastewater and Its Effective Correlation with Clinical Data during the Outbreak of COVID-19 in Salamanca. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25158071"},{"key":"ref_246","doi-asserted-by":"crossref","unstructured":"Trigo-Tasende, N., Vallejo, J.A., Rumbo-Feal, S., Conde-P\u00e9rez, K., Nasser-Ali, M., Tarr\u00edo-Saavedra, J., Barbeito, I., Lamelo, F., Cao, R., and Ladra, S. (2023). Building-Scale Wastewater-Based Epidemiology for SARS-CoV-2 Surveillance at Nursing Homes in A Coru\u00f1a, Spain. Environments, 10.","DOI":"10.3390\/environments10110189"},{"key":"ref_247","first-page":"16","article-title":"Wastewater monitoring of a community COVID-19 outbreak in a Spanish municipality","volume":"2","author":"Bijlsma","year":"2023","journal-title":"J. Environ. Expo. Assess."},{"key":"ref_248","doi-asserted-by":"crossref","first-page":"147463","DOI":"10.1016\/j.scitotenv.2021.147463","article-title":"Monitoring waves of the COVID-19 pandemic: Inferences from WWTPs of different sizes","volume":"787","author":"Zammit","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_249","doi-asserted-by":"crossref","first-page":"144704","DOI":"10.1016\/j.scitotenv.2020.144704","article-title":"Surveillance of SARS-CoV-2 spread using wastewater-based epidemiology: Comprehensive study","volume":"768","author":"Hemalatha","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_250","doi-asserted-by":"crossref","first-page":"402","DOI":"10.2166\/wh.2023.281","article-title":"Wastewater-based SARS-CoV-2 airport surveillance: Key trends at the Cape Town International Airport","volume":"21","author":"Nkambule","year":"2023","journal-title":"J. Water Health."},{"key":"ref_251","doi-asserted-by":"crossref","first-page":"111877","DOI":"10.1016\/j.envres.2021.111877","article-title":"Effect of selected wastewater characteristics on estimation of SARS-CoV-2 viral load in wastewater","volume":"203","author":"Amoah","year":"2022","journal-title":"Environ. Res."},{"key":"ref_252","doi-asserted-by":"crossref","unstructured":"Ngqwala, B., Msolo, L., Ebomah, K.E., Nontongana, N., and Okoh, A.I. (2024). Distribution of SARS-CoV-2 Genomes in Wastewaters and the Associated Potential Infection Risk for Plant Workers in Typical Urban and Peri-Urban Communities of the Buffalo City Region, South Africa. Viruses, 16.","DOI":"10.3390\/v16060871"},{"key":"ref_253","doi-asserted-by":"crossref","unstructured":"Tambe, L.A.M., Mathobo, P., Matume, N.D., Munzhedzi, M., Edokpayi, J.N., Viraragavan, A., Glanzmann, B., Tebit, D.M., Mavhandu-Ramarumo, L.G., and Street, R. (2023). Molecular epidemiology of SARS-CoV-2 in Northern South Africa: Wastewater surveillance from January 2021 to May 2022. Front. Public Health, 11.","DOI":"10.3389\/fpubh.2023.1309869"},{"key":"ref_254","doi-asserted-by":"crossref","first-page":"163785","DOI":"10.1016\/j.scitotenv.2023.163785","article-title":"Long-term study on wastewater SARS-CoV-2 surveillance across United Arab Emirates","volume":"887","author":"Wadi","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_255","doi-asserted-by":"crossref","unstructured":"Wilhelm, A., Agrawal, S., Schoth, J., Meinert-Berning, C., Bastian, D., Orschler, L., Ciesek, S., Teichgr\u00e4ber, B., Wintgens, T., and Lackner, S. (2022). Early Detection of SARS-CoV-2 Omicron BA.4 and BA.5 in German Wastewater. Viruses, 14.","DOI":"10.1101\/2022.07.27.22278003"},{"key":"ref_256","doi-asserted-by":"crossref","first-page":"157375","DOI":"10.1016\/j.scitotenv.2022.157375","article-title":"Wastewater surveillance allows early detection of SARS-CoV-2 omicron in North Rhine-Westphalia, Germany","volume":"846","author":"Wilhelm","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_257","doi-asserted-by":"crossref","first-page":"149031","DOI":"10.1016\/j.scitotenv.2021.149031","article-title":"Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich\u2014One year of experience","volume":"797","author":"Beyerl","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_258","doi-asserted-by":"crossref","unstructured":"Dumke, R., Geissler, M., Skupin, A., Helm, B., Mayer, R., Schubert, S., Oertel, R., Renner, B., and Dalpke, A.H. (2022). Simultaneous Detection of SARS-CoV-2 and Influenza Virus in Wastewater of Two Cities in Southeastern Germany, January to May 2022. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph192013374"},{"key":"ref_259","doi-asserted-by":"crossref","unstructured":"de la Cruz Barron, M., Kneis, D., Geissler, M., Dumke, R., Dalpke, A., and Berendonk, T.U. (2023). Evaluating the sensitivity of droplet digital PCR for the quantification of SARS-CoV-2 in wastewater. Front. Public Health, 11.","DOI":"10.3389\/fpubh.2023.1271594"},{"key":"ref_260","doi-asserted-by":"crossref","first-page":"165458","DOI":"10.1016\/j.scitotenv.2023.165458","article-title":"Analyzing community wastewater in sub-sewersheds for the small-scale detection of SARS-CoV-2 variants in a German metropolitan area","volume":"898","author":"Schmiege","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_261","doi-asserted-by":"crossref","unstructured":"Bartel, A., Grau, J.H., Bitzegeio, J., Werber, D., Linzner, N., Schumacher, V., Garske, S., Liere, K., Hackenbeck, T., and Rupp, S.I. (2024). Timely Monitoring of SARS-CoV-2 RNA Fragments in Wastewater Shows the Emergence of JN.1 (BA.2.86.1.1, Clade 23I) in Berlin, Germany. Viruses, 16.","DOI":"10.3390\/v16010102"},{"key":"ref_262","doi-asserted-by":"crossref","first-page":"143578","DOI":"10.1016\/j.scitotenv.2020.143578","article-title":"Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak","volume":"758","author":"Hata","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_263","doi-asserted-by":"crossref","first-page":"144587","DOI":"10.1016\/j.scitotenv.2020.144587","article-title":"Efficient detection of SARS-CoV-2 RNA in the solid fraction of wastewater","volume":"763","author":"Kitamura","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_264","doi-asserted-by":"crossref","first-page":"157613","DOI":"10.1016\/j.scitotenv.2022.157613","article-title":"Development of a magnetic nanoparticle-based method for concentrating SARS-CoV-2 in wastewater","volume":"848","author":"Angga","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_265","doi-asserted-by":"crossref","first-page":"165270","DOI":"10.1016\/j.scitotenv.2023.165270","article-title":"Long-term SARS-CoV-2 surveillance in wastewater and estimation of COVID-19 cases: An application of wastewater-based epidemiology","volume":"896","author":"Shrestha","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_266","doi-asserted-by":"crossref","first-page":"459","DOI":"10.2166\/wh.2022.183","article-title":"COVID-19 case prediction via wastewater surveillance in a low-prevalence urban community: A modeling approach","volume":"20","author":"Zhu","year":"2022","journal-title":"J. Water Health."},{"key":"ref_267","doi-asserted-by":"crossref","first-page":"167844","DOI":"10.1016\/j.scitotenv.2023.167844","article-title":"Development of highly sensitive one-step reverse transcription-quantitative PCR for SARS-CoV-2 detection in wastewater","volume":"907","author":"Raya","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_268","doi-asserted-by":"crossref","first-page":"150722","DOI":"10.1016\/j.scitotenv.2021.150722","article-title":"Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater","volume":"807","author":"Torii","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_269","doi-asserted-by":"crossref","unstructured":"Tanimoto, Y., Ito, E., Miyamoto, S., Mori, A., Nomoto, R., Nakanishi, N., Oka, N., Morimoto, T., and Iwamoto, T. (2022). SARS-CoV-2 RNA in Wastewater Was Highly Correlated with the Number of COVID-19 Cases During the Fourth and Fifth Pandemic Wave in Kobe City, Japan. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.892447"},{"key":"ref_270","doi-asserted-by":"crossref","first-page":"166526","DOI":"10.1016\/j.scitotenv.2023.166526","article-title":"Quantitative analysis of SARS-CoV-2 RNA in wastewater and evaluation of sampling frequency during the downward period of a COVID-19 wave in Japan","volume":"906","author":"Kuroita","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_271","doi-asserted-by":"crossref","first-page":"170103","DOI":"10.1016\/j.scitotenv.2024.170103","article-title":"Correspondence of SARS-CoV-2 genomic sequences obtained from wastewater samples and COVID-19 patient at long-term care facilities","volume":"916","author":"Kadoya","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_272","doi-asserted-by":"crossref","first-page":"4867626","DOI":"10.1155\/2022\/4867626","article-title":"A Simple Method to Detect SARS-CoV-2 in Wastewater at Low Virus Concentration","volume":"2022","author":"Thongpradit","year":"2022","journal-title":"J. Environ. Public Health."},{"key":"ref_273","first-page":"101815","article-title":"Surveillance of SARS-CoV-2 in sewage and wastewater treatment plants in Mexico","volume":"40","year":"2020","journal-title":"J. Water Process Eng."},{"key":"ref_274","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/s12560-021-09492-y","article-title":"Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico","volume":"13","author":"Betancourt","year":"2021","journal-title":"Food Environ. Virol."},{"key":"ref_275","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Reyes, J.R., Hern\u00e1ndez-Flores, M.d.l.L., Paredes-Zarco, J.E., T\u00e9llez-Jurado, A., Fayad-Meneses, O., and Carranza-Ram\u00edrez, L. (2021). Detection of SARS-CoV-2 in Wastewater Northeast of Mexico City: Strategy for Monitoring and Prevalence of COVID-19. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18168547"},{"key":"ref_276","doi-asserted-by":"crossref","unstructured":"Sosa-Hern\u00e1ndez, J.E., Oyervides-Mu\u00f1oz, M.A., Melchor-Mart\u00ednez, E.M., Driver, E.M., Bowes, D.A., Kraberger, S., Lucero-Saucedo, S.L., Fontenele, R.S., Parra-Arroyo, L., and Holland, L.A. (2022). Extensive Wastewater-Based Epidemiology as a Resourceful Tool for SARS-CoV-2 Surveillance in a Low-to-Middle-Income Country through a Successful Collaborative Quest: WBE, Mobility, and Clinical Tests. Water, 14.","DOI":"10.3390\/w14121842"},{"key":"ref_277","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.2166\/wh.2022.098","article-title":"The first detection of SARS-CoV-2 RNA in urban wastewater in Giza, Egypt","volume":"20","author":"Shaheen","year":"2022","journal-title":"J. Water Health."},{"key":"ref_278","doi-asserted-by":"crossref","first-page":"e16607","DOI":"10.1016\/j.heliyon.2023.e16607","article-title":"Is SARS-CoV-2 a concern in the largest wastewater treatment plant in middle east?","volume":"9","author":"Pasalari","year":"2023","journal-title":"Heliyon"},{"key":"ref_279","doi-asserted-by":"crossref","first-page":"148205","DOI":"10.1016\/j.scitotenv.2021.148205","article-title":"Moore swab performs equal to composite and outperforms grab sampling for SARS-CoV-2 monitoring in wastewater","volume":"790","author":"Rafiee","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_280","doi-asserted-by":"crossref","first-page":"159350","DOI":"10.1016\/j.scitotenv.2022.159350","article-title":"A 30-day follow-up study on the prevalence of SARS-COV-2 genetic markers in wastewater from the residence of COVID-19 patient and comparison with clinical positivity","volume":"858","author":"Islam","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_281","doi-asserted-by":"crossref","first-page":"161424","DOI":"10.1016\/j.scitotenv.2023.161424","article-title":"Dependency of sanitation infrastructure on the discharge of faecal coliform and SARS-CoV-2 viral RNA in wastewater from COVID and non-COVID hospitals in Dhaka, Bangladesh","volume":"867","author":"Amin","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_282","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s40246-023-00505-4","article-title":"Monitoring SARS-CoV-2 variants in wastewater of Dhaka City, Bangladesh: Approach to complement public health surveillance systems","volume":"17","author":"Haque","year":"2023","journal-title":"Hum. Genom."},{"key":"ref_283","doi-asserted-by":"crossref","unstructured":"Tharak, A., Kopperi, H., Hemalatha, M., Kiran, U., Gokulan, C.G., Moharir, S., Mishra, R.K., and Mohan, S.V. (2022). Longitudinal and Long-Term Wastewater Surveillance for COVID-19: Infection Dynamics and Zoning of Urban Community. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19052697"},{"key":"ref_284","doi-asserted-by":"crossref","first-page":"146252","DOI":"10.1016\/j.scitotenv.2021.146252","article-title":"First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India: Methods, occurrence and concurrence","volume":"778","author":"Chakraborty","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_285","doi-asserted-by":"crossref","first-page":"110946","DOI":"10.1016\/j.envres.2021.110946","article-title":"Unravelling the early warning capability of wastewater surveillance for COVID-19: A temporal study on SARS-CoV-2 RNA detection and need for the escalation","volume":"196","author":"Kumar","year":"2021","journal-title":"Environ. Res."},{"key":"ref_286","doi-asserted-by":"crossref","first-page":"141326","DOI":"10.1016\/j.scitotenv.2020.141326","article-title":"First proof of the capability of wastewater surveillance for COVID-19 in India through detection of genetic material of SARS-CoV-2","volume":"746","author":"Kumar","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_287","doi-asserted-by":"crossref","unstructured":"Arora, S., Nag, A., Rajpal, A., Tyagi, V.K., Tiwari, S.B., Sethi, J., Sutaria, D., Rajvanshi, J., Saxena, S., and Shrivastava, S.K. (2021). Imprints of Lockdown and Treatment Processes on the Wastewater Surveillance of SARS-CoV-2: A Curious Case of Fourteen Plants in Northern India. Water, 13.","DOI":"10.3390\/w13162265"},{"key":"ref_288","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.2166\/wh.2024.019","article-title":"Assessing the prevalence of FRNA bacteriophages and their correlation with SARS-CoV-2 RNA in the wastewater of Mumbai city","volume":"22","author":"Desai","year":"2024","journal-title":"J. Water Health."},{"key":"ref_289","doi-asserted-by":"crossref","first-page":"153816","DOI":"10.1016\/j.scitotenv.2022.153816","article-title":"Detection of SARS-CoV-2 RNA in wastewater, river water, and hospital wastewater of Nepal","volume":"824","author":"Tandukar","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_290","doi-asserted-by":"crossref","unstructured":"Shempela, D.M., Muleya, W., Mudenda, S., Daka, V., Sikalima, J., Kamayani, M., Sandala, D., Chipango, C., Muzala, K., and Musonda, K. (2024). Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool. Int. J. Mol. Sci., 25.","DOI":"10.20944\/preprints202406.1828.v1"},{"key":"ref_291","doi-asserted-by":"crossref","unstructured":"Dinssa, D.A., Gebremicael, G., Mengistu, Y., Hull, N.C., Chalchisa, D., Berhanu, G., Gebreegziabxier, A., Norberg, A., Snyder, S., and Wright, S. (2024). Longitudinal wastewater-based surveillance of SARS-CoV-2 during 2023 in Ethiopia. Front Public Health, 12.","DOI":"10.3389\/fpubh.2024.1394798"},{"key":"ref_292","doi-asserted-by":"crossref","unstructured":"Ali, S., Gudina, E.K., Gize, A., Aliy, A., Adankie, B.T., Tsegaye, W., Hundie, G.B., Muleta, M.B., Chibssa, T.R., and Belaineh, R. (2022). Community Wastewater-Based Surveillance Can Be a Cost-Effective Approach to Track COVID-19 Outbreak in Low-Resource Settings: Feasibility Assessment for Ethiopia Context. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19148515"},{"key":"ref_293","doi-asserted-by":"crossref","first-page":"9975781","DOI":"10.1155\/2024\/9975781","article-title":"First Molecular Detection of SARS-CoV-2 in Sewage and Wastewater in Ghana","volume":"2024","author":"Duker","year":"2024","journal-title":"BioMed Res. Int."},{"key":"ref_294","doi-asserted-by":"crossref","first-page":"148043","DOI":"10.1016\/j.scitotenv.2021.148043","article-title":"An alternative approach for bioanalytical assay optimization for wastewater-based epidemiology of SARS-CoV-2","volume":"789","author":"Boogaerts","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_295","doi-asserted-by":"crossref","unstructured":"Otero, M.C.B., Murao, L.A.E., Limen, M.A.G., Caalim, D.R.A., Gaite, P.L.A., Bacus, M.G., Acaso, J.T., Miguel, R.M., Corazo, K., and Knot, I.E. (2022). Multifaceted Assessment of Wastewater-Based Epidemiology for SARS-CoV-2 in Selected Urban Communities in Davao City, Philippines: A Pilot Study. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19148789"},{"key":"ref_296","doi-asserted-by":"crossref","first-page":"164519","DOI":"10.1016\/j.scitotenv.2023.164519","article-title":"Wastewater-based prediction of COVID-19 cases using a random forest algorithm with strain prevalence data: A case study of five municipalities in Latvia","volume":"891","author":"Dejus","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_297","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1007\/s11270-022-05772-w","article-title":"Wastewater-Based Epidemiology: Detection of SARS-CoV-2 RNA in Different Stages of Domestic Wastewater Treatment in Santa Fe, Argentina","volume":"233","author":"Reno","year":"2022","journal-title":"Water Air Soil Pollut."},{"key":"ref_298","doi-asserted-by":"crossref","first-page":"160573","DOI":"10.1016\/j.scitotenv.2022.160573","article-title":"Sewershed surveillance as a tool for smart management of a pandemic in threshold countries. Case study: Tracking SARS-CoV-2 during COVID-19 pandemic in a major urban metropolis in northwestern Argentina","volume":"862","author":"Cruz","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_299","doi-asserted-by":"crossref","first-page":"148887","DOI":"10.1016\/j.scitotenv.2021.148887","article-title":"Monitoring of SARS-CoV-2 RNA in wastewater as an epidemiological surveillance tool in Mendoza, Argentina","volume":"796","author":"Cuervo","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_300","doi-asserted-by":"crossref","first-page":"118541","DOI":"10.1016\/j.watres.2022.118541","article-title":"Wastewater based epidemiology as a silent sentinel of the trend of SARS-CoV-2 circulation in the community in central Argentina","volume":"219","author":"Masachessi","year":"2022","journal-title":"Water Res."},{"key":"ref_301","doi-asserted-by":"crossref","first-page":"7751","DOI":"10.1038\/s41598-024-58113-8","article-title":"Detection of SARS-COV-2 variants and their proportions in wastewater samples using next-generation sequencing in Finland","volume":"14","author":"Lipponen","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_302","doi-asserted-by":"crossref","first-page":"118220","DOI":"10.1016\/j.watres.2022.118220","article-title":"Detection and quantification of SARS-CoV-2 RNA in wastewater influent in relation to reported COVID-19 incidence in Finland","volume":"215","author":"Tiwari","year":"2022","journal-title":"Water Res."},{"key":"ref_303","doi-asserted-by":"crossref","first-page":"171401","DOI":"10.1016\/j.scitotenv.2024.171401","article-title":"Developing wastewater-based surveillance schemes for multiple pathogens: The WastPan project in Finland","volume":"926","author":"Tiwari","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_304","doi-asserted-by":"crossref","first-page":"118343","DOI":"10.1016\/j.watres.2022.118343","article-title":"Monitoring COVID-19 spread in Prague local neighborhoods based on the presence of SARS-CoV-2 RNA in wastewater collected throughout the sewer network","volume":"216","author":"Zdenkova","year":"2022","journal-title":"Water Res."},{"key":"ref_305","doi-asserted-by":"crossref","first-page":"161935","DOI":"10.1016\/j.scitotenv.2023.161935","article-title":"Monitoring COVID-19 spread in selected Prague\u2019s schools based on the presence of SARS-CoV-2 RNA in wastewater","volume":"871","author":"Zdenkova","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_306","first-page":"100220","article-title":"SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters","volume":"23","author":"Bencko","year":"2024","journal-title":"Water Res. X"},{"key":"ref_307","doi-asserted-by":"crossref","first-page":"100299","DOI":"10.1016\/j.lansea.2023.100299","article-title":"Environmental surveillance for COVID-19 using SARS-CoV-2 RNA concentration in wastewater\u2014A study in District East, Karachi, Pakistan","volume":"20","author":"Ansari","year":"2024","journal-title":"Lancet Reg. Health\u2014Southeast Asia"},{"key":"ref_308","doi-asserted-by":"crossref","unstructured":"Sharif, S., Ikram, A., Khurshid, A., Salman, M., Mehmood, N., Arshad, Y., Ahmed, J., Safdar, R.M., Rehman, L., and Mujtaba, G. (2021). Detection of SARs-CoV-2 in wastewater using the existing environmental surveillance network: A potential supplementary system for monitoring COVID-19 transmission. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0249568"},{"key":"ref_309","doi-asserted-by":"crossref","unstructured":"B\u0103icu\u0219, A., Cherciu, C.M., and Laz\u0103r, M. (2021). Identification of SARS-CoV-2 and Enteroviruses in Sewage Water\u2014A Pilot Study. Viruses, 13.","DOI":"10.3390\/v13050844"},{"key":"ref_310","doi-asserted-by":"crossref","first-page":"143226","DOI":"10.1016\/j.scitotenv.2020.143226","article-title":"Detection of SARS-CoV-2 RNA in hospital wastewater from a low COVID-19 disease prevalence area","volume":"755","author":"Koritnik","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_311","doi-asserted-by":"crossref","first-page":"120406","DOI":"10.1016\/j.watres.2023.120406","article-title":"Evaluation of wastewater-based epidemiology of COVID-19 approaches in Singapore\u2019s \u2018closed-system\u2019 scenario: A long-term country-wide assessment","volume":"244","author":"Caixia","year":"2023","journal-title":"Water Res."},{"key":"ref_312","doi-asserted-by":"crossref","first-page":"109029","DOI":"10.1016\/j.envint.2024.109029","article-title":"Wastewater genomic surveillance to track infectious disease-causing pathogens in low-income countries: Advantages, limitations, and perspectives","volume":"192","author":"Wardi","year":"2024","journal-title":"Environ. Int."},{"key":"ref_313","doi-asserted-by":"crossref","first-page":"147398","DOI":"10.1016\/j.scitotenv.2021.147398","article-title":"Ahead of the second wave: Early warning for COVID-19 by wastewater surveillance in Hungary","volume":"786","author":"Khayer","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_314","doi-asserted-by":"crossref","first-page":"e30267","DOI":"10.1016\/j.heliyon.2024.e30267","article-title":"Leveraging wastewater surveillance for managing the spread of SARS-CoV-2 and concerned pathogens during FIFA World Cup Qatar 2022","volume":"10","author":"Saththasivam","year":"2024","journal-title":"Heliyon"},{"key":"ref_315","doi-asserted-by":"crossref","first-page":"102775","DOI":"10.1016\/j.eti.2022.102775","article-title":"Application of human RNase P normalization for the realistic estimation of SARS-CoV-2 viral load in wastewater: A perspective from Qatar wastewater surveillance","volume":"27","author":"Saththasivam","year":"2022","journal-title":"Environ. Technol. Innov."},{"key":"ref_316","doi-asserted-by":"crossref","first-page":"110748","DOI":"10.1016\/j.envres.2021.110748","article-title":"Estimating the minimum number of SARS-CoV-2 infected cases needed to detect viral RNA in wastewater: To what extent of the outbreak can surveillance of wastewater tell us?","volume":"195","author":"Hong","year":"2021","journal-title":"Environ. Res."},{"key":"ref_317","doi-asserted-by":"crossref","first-page":"162466","DOI":"10.1016\/j.scitotenv.2023.162466","article-title":"SARS-CoV-2 wastewater-based epidemiology in an enclosed compound: A 2.5-year survey to identify factors contributing to local community dissemination","volume":"875","author":"Wang","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_318","first-page":"100082","article-title":"Long term detection and quantification of SARS-CoV-2 RNA in wastewater in Bahrain","volume":"7","author":"Naylor","year":"2022","journal-title":"J. Hazard. Mater. Adv."},{"key":"ref_319","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Aravena, M., Galb\u00e1n-Malag\u00f3n, C., Castro-Nallar, E., Barriga, G.P., Neira, V., Kr\u00fcger, L., Adell, A.D., and Olivares-Pacheco, J. (2024). Detection of SARS-CoV-2 in Wastewater Associated with Scientific Stations in Antarctica and Possible Risk for Wildlife. Microorganisms, 12.","DOI":"10.20944\/preprints202402.0240.v1"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/2\/205\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:26:51Z","timestamp":1770960411000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/2\/205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,4]]},"references-count":319,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["v18020205"],"URL":"https:\/\/doi.org\/10.3390\/v18020205","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,4]]}}}