{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T20:43:12Z","timestamp":1770410592234,"version":"3.49.0"},"reference-count":80,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Bacteriophages are promising antibacterial agents for managing acne vulgaris caused by Cutibacterium acnes, particularly given increasing antibiotic resistance. Here, we isolated and characterized two lytic Cutibacterium phages, NS-ph1 and NS-ph2, from acne lesions. Both bacteriophages exhibited a broad lytic spectrum, with a high activity against 27 C. acnes strains. Adsorption assays indicated rapid attachment and one-step growth experiments revealed latent periods of 4 h (NS-ph1) and 2 h (NS-ph2) and burst sizes of 70 and 59 PFU per infected cell, respectively. After long-term storage at room temperature, both phages retained infectivity for 3 months. Genome sequencing revealed linear dsDNA genomes of 29,490 bp (NS-ph1) and 29,189 bp (NS-ph2) with 51 and 46 predicted ORFs, respectively, and no tRNAs. No genes associated with lysogeny, toxins, or antibiotic resistance were detected. Comparative genomics placed both phages within the genus Pahexavirus. Together, these data expand the diversity of Pahexavirus and provide two well-characterized lytic candidates for further evaluation in anti-acne phage therapy.<\/jats:p>","DOI":"10.3390\/v18020214","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T10:05:47Z","timestamp":1770372347000},"page":"214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Genomic Characterization of Two Lytic Cutibacterium acnes Phages Defines Two Novel Pahexavirus Species"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7680-766X","authenticated-orcid":false,"given":"Anastasia A.","family":"Vorobeva","sequence":"first","affiliation":[{"name":"G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4724-2464","authenticated-orcid":false,"given":"Aleksei M.","family":"Vorobev","sequence":"additional","affiliation":[{"name":"G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1646-9802","authenticated-orcid":false,"given":"Peter V.","family":"Evseev","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emil R.","family":"Mekhtiev","sequence":"additional","affiliation":[{"name":"G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1377-7153","authenticated-orcid":false,"given":"Andrei V.","family":"Chaplin","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria A.","family":"Pasivkina","sequence":"additional","affiliation":[{"name":"G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina S.","family":"Zubkova","sequence":"additional","affiliation":[{"name":"G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana S.","family":"Bochkareva","sequence":"additional","affiliation":[{"name":"G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2911-4907","authenticated-orcid":false,"given":"Mikhail A.","family":"Yaitsky","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9626-2336","authenticated-orcid":false,"given":"Nikolai A.","family":"Nikitin","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia B.","family":"Demina","sequence":"additional","affiliation":[{"name":"A.P. Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8587-7965","authenticated-orcid":false,"given":"Victoria A.","family":"Korol","sequence":"additional","affiliation":[{"name":"A.P. Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrei V.","family":"Aleshkin","sequence":"additional","affiliation":[{"name":"Limited Liability Company Orphan-BIO, 354340 Sirius, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anatolij N.","family":"Blintsov","sequence":"additional","affiliation":[{"name":"Limited Liability Company Natura Siberica, 119571 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria N.","family":"Anurova","sequence":"additional","affiliation":[{"name":"G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"},{"name":"A.P. Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.tim.2022.10.006","article-title":"Cutibacterium\u00a0acnes","volume":"31","author":"Ahle","year":"2023","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Br\u00fcggemann, H., Salar-Vidal, L., Gollnick, H.P.M., and Lood, R. (2021). A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes. Front. 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