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There are no approved antiviral drugs for the treatment of infections with alphaviruses. Several alphaviruses are classified as risk group 3 agents which require handling in high-containment laboratories. To facilitate antiviral screening efforts against alphaviruses, we established a high-throughput antiviral screening assay using reporter Sindbis virus [SINV-GFP; expresses the green fluorescent protein] as a surrogate model for more pathogenic alphaviruses. The assay has strong reproducibility and was validated by reference small-molecule antivirals with various mechanisms of action. The use of high-content imaging as a readout, as demonstrated here, allows for high-throughput antiviral screening and provides a tool for early-stage antiviral discovery against emerging alphavirus threats at a lower biosafety level.<\/jats:p>","DOI":"10.3390\/v18030290","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T15:52:38Z","timestamp":1772207558000},"page":"290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A High-Throughput Antiviral Assay Based on a Sindbis Virus-GFP for the Discovery of Inhibitors of Alphavirus Replication"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2479-4429","authenticated-orcid":false,"given":"Gabriel Augusto","family":"Pires de Souza","sequence":"first","affiliation":[{"name":"VirusBank Platform, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, 3001 Leuven, 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Opin. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.trstmh.2008.07.025","article-title":"Impact of Climate Change and Other Factors on Emerging Arbovirus Diseases","volume":"103","author":"Gould","year":"2008","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/nature06536","article-title":"Global Trends in Emerging Infectious Diseases","volume":"451","author":"Jones","year":"2008","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1099\/jgv.0.001072","article-title":"ICTV Virus Taxonomy Profile: Togaviridae","volume":"99","author":"Chen","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/bs.aivir.2020.06.002","article-title":"Immunopathogenesis of Alphaviruses","volume":"107","author":"Baxter","year":"2020","journal-title":"Adv. Virus Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/nrmicro1006","article-title":"Transmission Cycles, Host Range, Evolution and Emergence of Arboviral Disease","volume":"2","author":"Weaver","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1099\/vir.0.82858-0","article-title":"Changing Patterns of Chikunya Virus: Re-Emergence of a Zoonotic Arbovirus","volume":"88","author":"Powers","year":"2007","journal-title":"J. Gen. Virol."},{"key":"ref_8","first-page":"1","article-title":"Arthralgia Resolution Rate Following Chikungunya Virus Infection","volume":"112","author":"Salje","year":"2021","journal-title":"Int. J. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.mcna.2008.07.008","article-title":"Chikungunya: A Paradigm of Emergence and Globalization of Vector-Borne Diseases","volume":"92","author":"Simon","year":"2008","journal-title":"Med. Clin. N. Am."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1056\/NEJMra1406035","article-title":"Chikungunya Virus and the Global Spread of a Mosquito-Borne Disease","volume":"372","author":"Weaver","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.antiviral.2014.11.001","article-title":"The Green Tea Catechin, Epigallocatechin Gallate Inhibits Chikungunya Virus Infection","volume":"113","author":"Weber","year":"2015","journal-title":"Antivir. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1128\/JVI.03213-14","article-title":"Mutations Conferring a Noncytotoxic Phenotype on Chikungunya Virus Replicons Compromise Enzymatic Properties of Nonstructural Protein 2","volume":"89","author":"Utt","year":"2015","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Pohjala, L., Utt, A., Varjak, M., Lulla, A., Merits, A., Ahola, T., and Tammela, P. (2011). Inhibitors of Alphavirus Entry and Replication Identified with a Stable Chikungunya Replicon Cell Line and Virus-Based Assays. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028923"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Broeckel, R., Sarkar, S., May, N.A., Totonchy, J., Kreklywich, C.N., Smith, P., Graves, L., DeFilippis, V.R., Heise, M.T., and Morrison, T.E. (2019). Src Family Kinase Inhibitors Block Translation of Alphavirus Subgenomic MRNAs. Antimicrob. Agents Chemother., 63.","DOI":"10.1128\/AAC.02325-18"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"113777","DOI":"10.1016\/j.bcp.2019.113777","article-title":"Antiviral Drug Discovery against Arthritogenic Alphaviruses: Tools and Molecular Targets","volume":"174","author":"Abdelnabi","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.virs.2023.06.007","article-title":"A High Throughput Antiviral Screening Platform for Alphaviruses Based on Semliki Forest Virus Expressing EGFP Reporter Gene","volume":"38","author":"Shi","year":"2023","journal-title":"Virol. Sin."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/mr.58.3.491-562.1994","article-title":"The Alphaviruses: Gene Expression, Replication, and Evolution","volume":"58","author":"Strauss","year":"1994","journal-title":"Microbiol. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/j.1365-2796.2004.01413.x","article-title":"Sindbis Viruses and Other Alphaviruses as Cause of Human Arthritic Disease","volume":"256","author":"Laine","year":"2004","journal-title":"J. Intern. Med."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Michie, A., Ernst, T., Pyke, A.T., Nicholson, J., Mackenzie, J.S., Smith, D.W., and Imrie, A. (2024). Genomic Analysis of Sindbis Virus Reveals Uncharacterized Diversity within the Australasian Region, and Support for Revised SINV Taxonomy. Viruses, 16.","DOI":"10.3390\/v16010007"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0042-6822(02)00139-3","article-title":"Human Immunodeficiency Virus Type 1 Envelope-Mediated Neuropathogenesis: Targeted Gene Delivery by a Sindbis Virus Expression Vector","volume":"309","author":"Ethier","year":"2003","journal-title":"Virology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1089\/vbz.2019.2562","article-title":"Sindbis Virus Strains of Divergent Origin Isolated from Humans and Mosquitoes During a Recent Outbreak in Finland","volume":"20","author":"Korhonen","year":"2020","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.1086\/430007","article-title":"Clinical and Laboratory Manifestations of Sindbis Virus Infection: Prospective Study, Finland, 2002-2003","volume":"191","author":"Kurkela","year":"2005","journal-title":"J. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3201\/eid1401.070510","article-title":"Sindbis Virus Infection in Resident Birds, Migratory Birds, and Humans, Finland","volume":"14","author":"Kurkela","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"100947","DOI":"10.1016\/j.onehlt.2024.100947","article-title":"First Isolation of the Sindbis Virus in Mosquitoes from Southwestern Spain Reveals a New Recent Introduction from Africa","volume":"20","author":"Ledesma","year":"2025","journal-title":"One Health"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Bakar, F.A., and Ng, L.F.P. (2018). Nonstructural Proteins of Alphavirus\u2014Potential Targets for Drug Development. Viruses, 10.","DOI":"10.3390\/v10020071"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"16534","DOI":"10.1073\/pnas.1210418109","article-title":"Structural and Functional Insights into Alphavirus Polyprotein Processing and Pathogenesis","volume":"109","author":"Shin","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Lello, L.S., Utt, A., Bartholomeeusen, K., Wang, S., Rausalu, K., Kendall, C., Coppens, S., Fragkoudis, R., Tuplin, A., and Alphey, L. (2020). Cross-Utilisation of Template RNAs by Alphavirus Replicases. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008825"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0035521","DOI":"10.1128\/JVI.00355-21","article-title":"NsP4 Is a Major Determinant of Alphavirus Replicase Activity and Template Selectivity","volume":"95","author":"Lello","year":"2021","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1186\/1743-422X-8-356","article-title":"Infectious Alphavirus Production from a Simple Plasmid Transfection+","volume":"8","author":"Steel","year":"2011","journal-title":"Virol. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays","volume":"4","author":"Zhang","year":"1999","journal-title":"J. Biomol. Screen."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s00216-010-3788-3","article-title":"The Beautiful Cell: High-Content Screening in Drug Discovery","volume":"398","author":"Bickle","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Mudhasani, R., Kota, K.P., Retterer, C., Tran, J.P., Whitehouse, C.A., and Bavari, S. (2014). High Content Image-Based Screening of a Protease Inhibitor Library Reveals Compounds Broadly Active against Rift Valley Fever Virus and Other Highly Pathogenic RNA Viruses. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0003095"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"105764","DOI":"10.1016\/j.antiviral.2023.105764","article-title":"High Content Screening Strategies for Large-Scale Compound Libraries with a Focus on High-Containment Viruses","volume":"221","author":"Subramani","year":"2024","journal-title":"Antivir. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1128\/jvi.67.6.3363-3374.1993","article-title":"Sindbis Virus Attachment: Isolation and Characterization of Mutants with Impaired Binding to Vertebrate Cells","volume":"67","author":"Dubuisson","year":"1993","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.cels.2023.05.008","article-title":"High-Throughput Discovery and Characterization of Viral Transcriptional Effectors in Human Cells","volume":"14","author":"Ludwig","year":"2023","journal-title":"Cell Syst."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1128\/MMBR.64.2.239-280.2000","article-title":"Translational Control of Viral Gene Expression in Eukaryotes","volume":"64","author":"Tan","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.virol.2003.08.012","article-title":"Translation Efficiency Determines Differences in Cellular Infection among Dengue Virus Type 2 Strains","volume":"317","author":"Edgil","year":"2003","journal-title":"Virology"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Pires De Souza, G.A., Le Bideau, M., Boschi, C., Wurtz, N., Colson, P., Aherfi, S., Devaux, C., and La Scola, B. (2022). Choosing a Cellular Model to Study SARS-CoV-2. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.1003608"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9031","DOI":"10.1128\/JVI.00850-09","article-title":"Random Insertion Mutagenesis of Sindbis Virus Nonstructural Protein 2 and Selection of Variants Incapable of Downregulating Cellular Transcription","volume":"83","author":"Frolov","year":"2009","journal-title":"J. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e01388-18","DOI":"10.1128\/JVI.01388-18","article-title":"Sindbis Virus Infection Causes Cell Death by NsP2-Induced Transcriptional Shutoff or by NsP3-Dependent Translational Shutoff","volume":"92","author":"Akhrymuk","year":"2018","journal-title":"J. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"199002","DOI":"10.1016\/j.virusres.2022.199002","article-title":"A Study of the MAYV Replication Cycle: Correlation between the Kinetics of Viral Multiplication and Viral Morphogenesis","volume":"323","author":"Reis","year":"2023","journal-title":"Virus Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e0173221","DOI":"10.1128\/JVI.01732-21","article-title":"In-Depth Characterization of the Chikungunya Virus Replication Cycle","volume":"9","author":"Reis","year":"2022","journal-title":"J. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Zheng, Y., Li, S., Song, K., Ye, J., Li, W., Zhong, Y., Feng, Z., Liang, S., Cai, Z., and Xu, K. (2022). A Broad Antiviral Strategy: Inhibitors of Human DHODH Pave the Way for Host-Targeting Antivirals against Emerging and Re-Emerging Viruses. Viruses, 14.","DOI":"10.3390\/v14050928"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Lucas-Hourani, M., Dauzonne, D., Jorda, P., Cousin, G., Lupan, A., Helynck, O., Caignard, G., Janvier, G., Andr\u00e9-Leroux, G., and Khiar, S. (2013). Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003678"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.antiviral.2019.01.003","article-title":"Approved Drugs Screening against the NsP1 Capping Enzyme of Venezuelan Equine Encephalitis Virus Using an Immuno-Based Assay","volume":"163","author":"Li","year":"2019","journal-title":"Antivir. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"106186","DOI":"10.1016\/j.antiviral.2025.106186","article-title":"Combinations of Approved Oral Nucleoside Analogues Confer Potent Suppression of Alphaviruses in Vitro and in Vivo","volume":"239","author":"Verwimp","year":"2025","journal-title":"Antivir. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e01965-17","DOI":"10.1128\/JVI.01965-17","article-title":"\u03b2-d-N4-Hydroxycytidine Is a Potent Anti-Alphavirus Compound That Induces a High Level of Mutations in the Viral Genome","volume":"92","author":"Urakova","year":"2018","journal-title":"J. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"105858","DOI":"10.1016\/j.antiviral.2024.105858","article-title":"\u03b2-D-N4-Hydroxycytidine (NHC, EIDD-1931) Inhibits Chikungunya Virus Replication in Mosquito Cells and Ex Vivo Aedes Aegypti Guts, but Not When Ingested during Blood-Feeding","volume":"225","author":"Soto","year":"2024","journal-title":"Antivir. Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Langendries, L., Abdelnabi, R., Neyts, J., and Delang, L. (2021). Repurposing Drugs for Mayaro Virus: Identification of Eidd-1931, Favipiravir and Suramin as Mayaro Virus Inhibitors. Microorganisms, 9.","DOI":"10.3390\/microorganisms9040734"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e02395-16","DOI":"10.1128\/AAC.02395-16","article-title":"Characterization of \u03b2-D-N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus","volume":"61","author":"Ehteshami","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5883","DOI":"10.1126\/scitranslmed.abb5883","article-title":"An Orally Bioavailable Broad-Spectrum Antiviral Inhibits SARS-CoV-2 in Human Airway Epithelial Cell Cultures and Multiple Coronaviruses in Mice","volume":"12","author":"Sheahan","year":"2020","journal-title":"Sci. Transl. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1099\/0022-1317-83-7-1535","article-title":"Dissecting Virus Entry via Endocytosis","volume":"83","author":"Sieczkarski","year":"2002","journal-title":"J. Gen. Virol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ijantimicag.2007.05.015","article-title":"Recycling of Chloroquine and Its Hydroxyl Analogue to Face Bacterial, Fungal and Viral Infections in the 21st Century","volume":"30","author":"Rolain","year":"2007","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1089\/vbz.2008.0049","article-title":"Le On Chikungunya Acute Infection and Chloroquine Treatment","volume":"8","author":"Boisson","year":"2008","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.3390\/v7072792","article-title":"Early Events in Chikungunya Virus Infection\u2014From Virus Cell Binding to Membrane Fusion","volume":"7","author":"Hoornweg","year":"2015","journal-title":"Viruses"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/3\/290\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T05:17:01Z","timestamp":1772342221000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/3\/290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,27]]},"references-count":55,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["v18030290"],"URL":"https:\/\/doi.org\/10.3390\/v18030290","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,27]]}}}