{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T05:57:41Z","timestamp":1772517461566,"version":"3.50.1"},"reference-count":36,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:00:00Z","timestamp":1772236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004417","name":"Global Fund","doi-asserted-by":"crossref","award":["FA_202400455"],"award-info":[{"award-number":["FA_202400455"]}],"id":[{"id":"10.13039\/100004417","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Wellcome Trust Hosts, Pathogens & Global Health programme","award":["218471\/Z\/19\/Z"],"award-info":[{"award-number":["218471\/Z\/19\/Z"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Dengue is the leading mosquito-borne viral cause of human illness and death. More than four billion people globally are at risk of dengue virus (DENV) infection, and most infections are asymptomatic or present with a non-specific febrile illness. We characterise the first complete DENV-2 genome from Sierra Leone, recovered from a febrile adult who tested RT-PCR\u2013positive. The sequence was identified as DENV-2 genotype II, lineage F.1.1. Phylogenetically, the Sierra Leone genome formed a well-supported sister lineage with a 2024 USA DENV-2 genome; both were nested within but clearly diverged from Indian DENV-2 sequences (2021\u20132022) and were distinct from the R\u00e9union DENV-2 clade. The degree of genetic divergence was incompatible with a recent or direct import of a South Asian lineage and was more consistent with diversification in an under-sampled Indian Ocean\/South Asia network or outside this region in Africa. With a single Sierra Leone genome, the source and extent of local transmission remain unresolved. These findings underscore the benefits of integrating differential diagnostics and genomics into routine care for febrile illness and sustaining regional arboviral surveillance.<\/jats:p>","DOI":"10.3390\/v18030298","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T14:55:01Z","timestamp":1772463301000},"page":"298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterisation of the First Complete Dengue Genome in Sierra Leone"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9178-1556","authenticated-orcid":false,"given":"Allan K. O.","family":"Campbell","sequence":"first","affiliation":[{"name":"Central Public Health Reference Laboratory, National Public Health Agency, Freetown P.O. Box 529, Sierra Leone"},{"name":"National Public Health Agency, Ministry of Health, Freetown P.O. Box 529, Sierra Leone"},{"name":"Faculty of Medical Laboratory Sciences and Diagnostics, College of Medicine and Allied Health Sciences, Freetown P.O. Box 529, Sierra Leone"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ifeanyi","family":"Omah","sequence":"additional","affiliation":[{"name":"Institute of Ecology and Evolution, University of Edinburgh, Edinburgh EH9 3FL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andy M.","family":"Diouf","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar 22000, Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1030-3796","authenticated-orcid":false,"given":"Mignane","family":"Ndiaye","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar 22000, Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian S. O.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Central Public Health Reference Laboratory, National Public Health Agency, Freetown P.O. Box 529, Sierra Leone"},{"name":"Faculty of Medical Laboratory Sciences and Diagnostics, College of Medicine and Allied Health Sciences, Freetown P.O. Box 529, Sierra Leone"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edyth","family":"Parker","sequence":"additional","affiliation":[{"name":"Institute of Genomics and Global Health, Redeemer\u2019s University, Ede 232101, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vidalyn","family":"Folorunso","sequence":"additional","affiliation":[{"name":"Central Public Health Reference Laboratory, National Public Health Agency, Freetown P.O. 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Box 529, Sierra Leone"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Daniel-Sesay","sequence":"additional","affiliation":[{"name":"Central Public Health Reference Laboratory, National Public Health Agency, Freetown P.O. Box 529, Sierra Leone"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zein","family":"Souma","sequence":"additional","affiliation":[{"name":"Central Public Health Reference Laboratory, National Public Health Agency, Freetown P.O. Box 529, Sierra Leone"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Choe","family":"Miller","sequence":"additional","affiliation":[{"name":"Central Public Health Reference Laboratory, National Public Health Agency, Freetown P.O. 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