{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T14:00:44Z","timestamp":1774274444960,"version":"3.50.1"},"reference-count":28,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T00:00:00Z","timestamp":1774051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Cytomegalovirus (CMV) remains a major opportunistic pathogen in individuals with HIV. The aim of this study was to investigate the seroprevalence and reactivation rates of CMV among HIV-positive individuals. A total of 300 people with HIV presenting to the Istanbul Faculty of Medicine were enrolled. Serological assessments were performed using enzyme-linked immunosorbent assay (ELISA), while molecular analyses were conducted through PCR-based methods. Sociodemographic and clinical characteristics of the patients were also evaluated. Of the participants, 90% were male, with an age range of 18\u201376 years. Serological testing demonstrated CMV IgG positivity in 292 patients (97.3%) and CMV IgM positivity in 11 patients (4.07%). CMV DNA was detected in 91 patients (30.3%) by molecular assays, with viral loads ranging from &lt;150 to 2,404,678 copies\/mL. CMV DNA positivity was significantly more frequent in older patients (p &lt; 0.05) and was associated with lower CD4+ T lymphocyte counts. CMV disease was identified in 50 patients (16.7%), with organ involvement (64%) representing the most common clinical manifestation. CMV seropositivity is remarkably high in HIV-positive individuals, and reactivation rates are increased, particularly in older patients and those with advanced immunosuppression. These findings underscore the clinical relevance of routine CMV surveillance in the management of HIV infection.<\/jats:p>","DOI":"10.3390\/v18030394","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T13:07:06Z","timestamp":1774271226000},"page":"394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seroepidemiology and Reactivation Rates of Cytomegalovirus in HIV-Positive Patients in Istanbul: A Retrospective Analysis"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0526-6759","authenticated-orcid":false,"given":"Derya","family":"Sevimli Saydan","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat Hakan","family":"Kir","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8411-2795","authenticated-orcid":false,"given":"Muammer Osman","family":"K\u00f6ksal","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6487-102X","authenticated-orcid":false,"given":"Kutay","family":"Sarsar","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0592-7297","authenticated-orcid":false,"given":"Arat","family":"Hulikyan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atahan","family":"Cagatay","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9670-2426","authenticated-orcid":false,"given":"Mehmet","family":"Demirci","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, K\u0131rklareli University, 39100 K\u0131rklareli, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u0131nar","family":"Soguksu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0565-6407","authenticated-orcid":false,"given":"Eray","family":"Yurtseven","sequence":"additional","affiliation":[{"name":"Department of Biostatics, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4263-237X","authenticated-orcid":false,"given":"Serra Zeynep","family":"Akkoyunlu","sequence":"additional","affiliation":[{"name":"Department of Biostatics, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-0180","authenticated-orcid":false,"given":"Sevim","family":"Me\u015fe","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Agacfidan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2476-3796","authenticated-orcid":false,"given":"Hayriye","family":"Kirkoyun Uysal","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,21]]},"reference":[{"key":"ref_1","unstructured":"Baron, S. 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