{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:41:01Z","timestamp":1785426061033,"version":"3.56.0"},"reference-count":91,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006756","name":"Brigham Young University","doi-asserted-by":"publisher","award":["not applicable"],"award-info":[{"award-number":["not applicable"]}],"id":[{"id":"10.13039\/100006756","id-type":"DOI","asserted-by":"publisher"}]},{"award":["not applicable"],"award-info":[{"award-number":["not applicable"]}],"id":[{"id":"https:\/\/ror.org\/047rhhm47","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The rate at which bacteria are gaining resistance to antibiotics is outpacing the discovery of new drugs. The rise of superbugs such as Carbapenem-resistant and Extended-Spectrum Beta-Lactamase Producing Enterobacteriaceae are leading to infections that are resistant to our last lines of defense. One of the most prolific genera of these bacteria is Klebsiella, which causes one third of Gram-negative infections. The need for alternative and companion treatments has never been greater. Bacteriophages are bacteria-infecting viruses with high specificity to their host. They show great promise as a potential treatment for antibiotic-resistant infections. Here, we describe the characterization of five closely related bacteriophages (ValerieMcCarty01\u201305) isolated against an antibiotic-resistant clinical strain of Klebsiella oxytoca, which is an emerging antimicrobial-resistant threat within the Klebsiella genus. These phages demonstrate high similarity at both the genomic and proteomic levels and share homology with other T4-like Enterobacterales phage. Two phages were further characterized through a mass spectrometry analysis of purified virions, identifying peptide spectrum matches for 40 proteins which appear to be virion proteins. In addition, the peptide spectrum matches for 39 hypothetical proteins suggest they are indeed proteins. Amino acid alignment revealed that the tail fibers display more variability than most of their genome, suggesting possible adaptive tail fiber gene shuffling. Despite this variability, these phages maintained broad but high specificity for Klebsiella species in this paper, including K. oxytoca, K. pneumoniae and K. aerogenes and several clinical Klebsiella isolates, with infectivity differences seen only in efficiency. This specificity for Klebsiella is consistent with the genus to which they belong (the Jiaodavirus, which contains only Klebsiella phages) and suggests they may be involved in the evolution of Klebsiella and be useful therapeutics.<\/jats:p>","DOI":"10.3390\/v18040430","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T07:57:55Z","timestamp":1775116675000},"page":"430","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of Klebsiella Phages Isolated Against a Clinical Host with High Genome and Proteome Identity but Variable Tail Fibers"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9562-4673","authenticated-orcid":false,"given":"Jessica M.","family":"Lewis","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84040, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9812-149X","authenticated-orcid":false,"given":"Daniel K.","family":"Arens","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84040, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan R.","family":"Zuniga","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Brigham Young University, Provo, UT 84040, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8816-6635","authenticated-orcid":false,"given":"Julianne H.","family":"Grose","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84040, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Aminov, R.I. (2010). A brief history of the antibiotic era: Lessons learned and challenges for the future. Front. Microbiol., 1.","DOI":"10.3389\/fmicb.2010.00134"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"E40","DOI":"10.1016\/S1473-3099(18)30513-9","article-title":"Analysis of the clinical antibacterial and antituberculosis pipeline","volume":"19","author":"Theuretzbacher","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1111\/j.1476-5381.2011.01250.x","article-title":"Novel classes of antibiotics or more of the same?","volume":"163","author":"Coates","year":"2011","journal-title":"Br. J. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"da Cunha, B.R., Fonseca, L.P., and Calado, C.R.C. (2019). Antibiotic Discovery: Where Have We Come from, Where Do We Go?. Antibiotics, 8.","DOI":"10.3390\/antibiotics8020045"},{"key":"ref_5","unstructured":"(2026, March 26). 2019 Antibiotic Resistance Threats Report, Available online: https:\/\/www.cdc.gov\/antimicrobial-resistance\/data-research\/threats\/index.html."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1016\/S0140-6736(88)92919-4","article-title":"Fluoconazole resistance in candida glabrata","volume":"2","author":"Warnock","year":"1988","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/S0140-6736(67)92346-X","article-title":"A resistant pneumococcus","volume":"2","author":"Hansman","year":"1967","journal-title":"Lancet"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/S0140-6736(76)92466-1","article-title":"Beta-lactamase-producing, penicillin-resistant gonococcus","volume":"308","author":"Phillips","year":"1976","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1136\/bmj.1.5219.124-a","article-title":"celbenin-resistant staphylococci","volume":"1","author":"Jevons","year":"1961","journal-title":"Br. Med. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/nature10388","article-title":"Antibiotic resistance is ancient","volume":"477","author":"King","year":"2011","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1101\/cshperspect.a025197","article-title":"The Prehistory of Antibiotic Resistance","volume":"6","author":"Perry","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"13803","DOI":"10.1038\/ncomms13803","article-title":"A diverse intrinsic antibiotic resistome from a cave bacterium","volume":"7","author":"Pawlowski","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1111\/1758-2229.12453","article-title":"Exploring divergent antibiotic resistance genes in ancient metagenomes and discovery of a novel beta-lactamase family","volume":"8","author":"Rascovan","year":"2016","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_14","unstructured":"(2025, February 12). WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789240093461."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1093\/gbe\/evx019","article-title":"Population structure of multidrug resistant Klebsiella oxytoca within hospitals across the UK and Ireland identifies sharing of virulence and resistance genes with K. pneumoniae","volume":"9","author":"Moradigaravand","year":"2017","journal-title":"Genome Biol. Evol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"64","DOI":"10.12703\/P6-64","article-title":"Klebsiella: A long way to go towards understanding this enigmatic jet-setter","volume":"6","author":"Broberg","year":"2014","journal-title":"F1000Prime Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"S59","DOI":"10.1016\/j.mjafi.2016.05.002","article-title":"Klebsiella oxytoca: An emerging pathogen?","volume":"72","author":"Singh","year":"2016","journal-title":"Med. J. Armed Forces India"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.resmic.2008.05.001","article-title":"Exploring the prokaryotic virosphere","volume":"159","author":"Comeau","year":"2008","journal-title":"Res. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/bies.201400152","article-title":"A century of phage research: Bacteriophages and the shaping of modern biology","volume":"37","author":"Keen","year":"2015","journal-title":"Bioessays"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1016\/j.cell.2017.10.045","article-title":"Contemporary Phage Biology: From Classic Models to New Insights","volume":"172","author":"Ofir","year":"2018","journal-title":"Cell"},{"key":"ref_21","first-page":"23","article-title":"Antibacterial applications of bacteriophages","volume":"24","author":"Ansaldi","year":"2020","journal-title":"Virologie"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Lawrence, D., Baldridge, M.T., and Handley, S.A. (2019). Phages and Human Health: More Than Idle Hitchhikers. Viruses, 11.","DOI":"10.3390\/v11070587"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2174\/1567201813666160406115744","article-title":"An Overview on Bacteriophages: A Natural Nanostructured Antibacterial Agent","volume":"15","author":"Rastogi","year":"2018","journal-title":"Curr. Drug Deliv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"104048","DOI":"10.1016\/j.micpath.2020.104048","article-title":"Therapeutic applications of lytic phages in human medicine","volume":"142","author":"Guo","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"279","DOI":"10.5694\/mja2.50355","article-title":"Phage therapy for severe bacterial infections: A narrative review","volume":"212","author":"Fabijan","year":"2020","journal-title":"Med. J. Aust."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1111\/jam.14535","article-title":"Therapeutic bacteriophages as a rescue treatment for drug-resistant infections\u2014An in vivo studies overview","volume":"128","author":"Kwiatek","year":"2020","journal-title":"J. Appl. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"a023879","DOI":"10.1101\/cshperspect.a023879","article-title":"Phage Therapy in the Era of Synthetic Biology","volume":"8","author":"Barbu","year":"2016","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0083623","DOI":"10.1128\/MRA.00836-23","article-title":"Genome sequences of two Klebsiella phages isolated from wastewater treatment samples that infect a clinical Klebsiella isolate","volume":"14","author":"Lewis","year":"2025","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_30","unstructured":"Lawrence, J. (2022, January 05). DNA Master. Available online: http:\/\/cobamide2.bio.pitt.edu\/computer.htm."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1093\/nar\/29.12.2607","article-title":"GeneMarkS: A self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions","volume":"29","author":"Besemer","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: A new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.1111\/j.1742-4658.2005.04945.x","article-title":"Protein database searches using compositionally adjusted substitution matrices","volume":"272","author":"Altschul","year":"2005","journal-title":"FEBS J."},{"key":"ref_35","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Ram","year":"2004","journal-title":"Biophotonics Int."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1093\/nar\/gkn1006","article-title":"Kalign2: High-performance multiple alignment of protein and nucleotide sequences allowing external features","volume":"37","author":"Lassmann","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1093\/bioinformatics\/btm039","article-title":"Gepard: A rapid and sensitive tool for creating dotplots on genome scale","volume":"23","author":"Krumsiek","year":"2007","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Moraru, C. (2023). VirClust-A Tool for Hierarchical Clustering, Core Protein Detection and Annotation of (Prokaryotic) Viruses. Viruses, 15.","DOI":"10.3390\/v15041007"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1093\/bioinformatics\/btm076","article-title":"COBALT: Constraint-based alignment tool for multiple protein sequences","volume":"23","author":"Papadopoulos","year":"2007","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1093\/bib\/bbn013","article-title":"Recent developments in the MAFFT multiple sequence alignment program","volume":"9","author":"Katoh","year":"2008","journal-title":"Brief. Bioinform."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/(SICI)1097-0134(19980701)32:1<88::AID-PROT10>3.0.CO;2-J","article-title":"Generalized affine gap costs for protein sequence alignment","volume":"32","author":"Altschul","year":"1998","journal-title":"Proteins"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"EMBOSS: The European Molecular Biology Open Software Suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"M111 010587","DOI":"10.1074\/mcp.M111.010587","article-title":"PEAKS DB: De novo sequencing assisted database search for sensitive and accurate peptide identification","volume":"11","author":"Zhang","year":"2012","journal-title":"Mol. Cell. Proteom."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e85183","DOI":"10.7554\/eLife.85183","article-title":"Phage tRNAs evade tRNA-targeting host defenses through anticodon loop mutations","volume":"12","author":"Costa","year":"2023","journal-title":"eLife"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1101\/gr.6649807","article-title":"Causes for the intriguing presence of tRNAs in phages","volume":"17","author":"Vergassola","year":"2007","journal-title":"Genome Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e1219441","DOI":"10.1080\/21597081.2016.1219441","article-title":"Testing hypotheses for the presence of tRNA genes in mycobacteriophage genomes","volume":"6","author":"Delesalle","year":"2016","journal-title":"Bacteriophage"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.virol.2014.08.024","article-title":"Understanding the enormous diversity of bacteriophages: The tailed phages that infect the bacterial family Enterobacteriaceae","volume":"468","author":"Grose","year":"2014","journal-title":"Virology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s00705-022-05694-2","article-title":"Abolishment of morphology-based taxa and change to binomial species names: 2022 taxonomy update of the ICTV bacterial viruses subcommittee","volume":"168","author":"Turner","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e00919-17","DOI":"10.1128\/genomeA.00919-17","article-title":"Complete Annotated Genome Sequences of Four Klebsiella pneumoniae Phages Isolated from Sewage in Poland","volume":"5","author":"Labudda","year":"2017","journal-title":"Genome Announc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.jmb.2010.01.011","article-title":"Comparative genomic analysis of 60 Mycobacteriophage genomes: Genome clustering, gene acquisition, and gene size","volume":"397","author":"Hatfull","year":"2010","journal-title":"J. Mol. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Adriaenssens, E., and Brister, J.R. (2017). How to Name and Classify Your Phage: An Informal Guide. Viruses, 9.","DOI":"10.1101\/111526"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3035","DOI":"10.1111\/j.1462-2920.2010.02280.x","article-title":"Genomic analysis of oceanic cyanobacterial myoviruses compared with T4-like myoviruses from diverse hosts and environments","volume":"12","author":"Sullivan","year":"2010","journal-title":"Environ. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1093\/bioinformatics\/btz795","article-title":"Kalign 3: Multiple sequence alignment of large data sets","volume":"36","author":"Lassmann","year":"2019","journal-title":"Bioinformatics"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Lassmann, T., and Sonnhammer, E.L. (2005). Kalign--an accurate and fast multiple sequence alignment algorithm. BMC Bioinform., 6.","DOI":"10.1186\/1471-2105-6-298"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.virol.2006.12.031","article-title":"Modular architecture of the T4 phage superfamily: A conserved core genome and a plastic periphery","volume":"362","author":"Comeau","year":"2007","journal-title":"Virology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1093\/molbev\/msl036","article-title":"A selective barrier to horizontal gene transfer in the T4-type bacteriophages that has preserved a core genome with the viral replication and structural genes","volume":"23","author":"Filee","year":"2006","journal-title":"Mol. Biol. Evol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zafar, N., Mazumder, R., and Seto, D. (2002). CoreGenes: A computational tool for identifying and cataloging \u201ccore\u201d genes in a set of small genomes. BMC Bioinform., 3.","DOI":"10.1186\/1471-2105-3-12"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Ciacci, N., D\u2019Andrea, M.M., Marmo, P., Dematte, E., Amisano, F., Di Pilato, V., Fraziano, M., Lupetti, P., Rossolini, G.M., and Thaller, M.C. (2018). Characterization of vB_Kpn_F48, a Newly Discovered Lytic Bacteriophage for Klebsiella pneumoniae of Sequence Type 101. Viruses, 10.","DOI":"10.3390\/v10090482"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e00255-19","DOI":"10.1128\/MRA.00255-19","article-title":"Complete Genome Sequence of Salmonella enterica Serovar Newport Myophage Melville","volume":"8","author":"Zhang","year":"2019","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e01427-14","DOI":"10.1128\/genomeA.01427-14","article-title":"Complete Genome Sequence of Citrobacter freundii Myophage Moon","volume":"3","author":"Edwards","year":"2015","journal-title":"Genome Announc."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e01137-15","DOI":"10.1128\/genomeA.01137-15","article-title":"Complete Genome Sequence of Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Myophage Miro","volume":"3","author":"Mijalis","year":"2015","journal-title":"Genome Announc."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1111\/j.1365-2958.2008.06344.x","article-title":"The headful packaging nuclease of bacteriophage T4","volume":"69","author":"Alam","year":"2008","journal-title":"Mol. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/B978-0-12-394621-8.00018-2","article-title":"Structure, assembly, and DNA packaging of the bacteriophage T4 head","volume":"82","author":"Black","year":"2012","journal-title":"Adv. Virus Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1006\/jmbi.1996.0291","article-title":"A discontinuous headful packaging model for packaging less than headful length DNA molecules by bacteriophage T4","volume":"258","author":"Leffers","year":"1996","journal-title":"J. Mol. Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e00648","DOI":"10.1128\/mBio.00648-15","article-title":"Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9","volume":"6","author":"Bryson","year":"2015","journal-title":"mBio"},{"key":"ref_69","unstructured":"Tabor, S. (2001). RNA ligases. Curr. Protoc. Mol. Biol., 3."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0092-8674(05)80091-1","article-title":"Bacteriophage T4 Alc protein: A transcription termination factor sensing local modification of DNA","volume":"75","author":"Kashlev","year":"1993","journal-title":"Cell"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"7262","DOI":"10.1128\/JB.186.21.7262-7272.2004","article-title":"ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: Catalytic properties and mutation analysis","volume":"186","author":"Tiemann","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1038\/s41579-018-0070-8","article-title":"Targeting mechanisms of tailed bacteriophages","volume":"16","author":"Nobrega","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1186\/1743-422X-7-355","article-title":"Morphogenesis of the T4 tail and tail fibers","volume":"7","author":"Leiman","year":"2010","journal-title":"Virol. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e00257-19","DOI":"10.1128\/MRA.00257-19","article-title":"Complete Genome Sequence of Klebsiella pneumoniae Myophage Mineola","volume":"8","author":"Boeckman","year":"2019","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e0031123","DOI":"10.1128\/MRA.00311-23","article-title":"Isolation and genome sequencing of five lytic bacteriophages from hospital wastewater in the Philippines","volume":"12","author":"Nada","year":"2023","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e0050622","DOI":"10.1128\/aac.00506-22","article-title":"A Lysozyme Murein Hydrolase with Broad-Spectrum Antibacterial Activity from Enterobacter Phage myPSH1140","volume":"66","author":"Ramesh","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"199025","DOI":"10.1016\/j.virusres.2022.199025","article-title":"Characterization and the host specificity of Pet-CM3-4, a new phage infecting Cronobacter and Enterobacter strains","volume":"324","author":"Andrezal","year":"2023","journal-title":"Virus Res."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Petrzik, K., Brazdova, S., and Krawczyk, K. (2021). Novel Viruses That Lyse Plant and Human Strains of Kosakonia cowanii. Viruses, 13.","DOI":"10.3390\/v13081418"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1007\/s00253-023-12547-8","article-title":"CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers","volume":"107","author":"Oliveira","year":"2023","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Cao, Y., Ma, R., Li, Z., Mao, X., Li, Y., Wu, Y., Wang, L., Han, K., Li, L., and Ma, D. (2022). Broad-Spectrum Salmonella Phages PSE-D1 and PST-H1 Controls Salmonella in Foods. Viruses, 14.","DOI":"10.3390\/v14122647"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1128\/IAI.68.2.953-955.2000","article-title":"Capsular polysaccharide is a major complement resistance factor in lipopolysaccharide O side chain-deficient Klebsiella pneumoniae clinical isolates","volume":"68","author":"Alvarez","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1111\/cmi.12466","article-title":"Klebsiella pneumoniae survives within macrophages by avoiding delivery to lysosomes","volume":"17","author":"Cano","year":"2015","journal-title":"Cell. Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.2147\/IDR.S460155","article-title":"Study of the Epidemiological and Mechanistic Differences Between Carbapenem-Resistant Klebsiella pneumoniae Infections in Children and Adults","volume":"17","author":"Zhou","year":"2024","journal-title":"Infect. Drug Resist."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Fevre, C., Passet, V., Deletoile, A., Barbe, V., Frangeul, L., Almeida, A.S., Sansonetti, P., Tournebize, R., and Brisse, S. (2011). PCR-based identification of Klebsiella pneumoniae subsp. rhinoscleromatis, the agent of rhinoscleroma. PLoS Neglected Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001052"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1099\/jmm.0.015198-0","article-title":"PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets","volume":"59","author":"Turton","year":"2010","journal-title":"J. Med. Microbiol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1086\/499968","article-title":"Genetic determinants of capsular serotype K1 of Klebsiella pneumoniae causing primary pyogenic liver abscess","volume":"193","author":"Chuang","year":"2006","journal-title":"J. Infect. Dis."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"4239","DOI":"10.1128\/JCM.05297-11","article-title":"IMP-producing carbapenem-resistant Klebsiella pneumoniae in the United States","volume":"49","author":"Limbago","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Pan, Y.J., Lin, T.L., Chen, Y.H., Hsu, C.R., Hsieh, P.F., Wu, M.C., and Wang, J.T. (2013). Capsular types of Klebsiella pneumoniae revisited by wzc sequencing. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0080670"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1038\/s41598-020-58012-8","article-title":"Pathogenomics and Evolutionary Epidemiology of Multi-Drug Resistant Clinical Klebsiella pneumoniae Isolated from Pretoria, South Africa","volume":"10","author":"Mbelle","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"e1251379","DOI":"10.1080\/21597081.2016.1251379","article-title":"Phagebiotics in treatment and prophylaxis of healthcare-associated infections","volume":"6","author":"Aleshkin","year":"2016","journal-title":"Bacteriophage"},{"key":"ref_91","first-page":"758","article-title":"Cross-reactivation differences in bacteriophage T4D","volume":"26","author":"Womack","year":"1965","journal-title":"Virology"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/4\/430\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T03:13:51Z","timestamp":1775445231000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/4\/430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,1]]},"references-count":91,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["v18040430"],"URL":"https:\/\/doi.org\/10.3390\/v18040430","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,1]]}}}