{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:35:15Z","timestamp":1789623315202,"version":"build-2803163510"},"reference-count":55,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100031746","name":"Science for Africa Foundation","doi-asserted-by":"crossref","award":["DEL-22-012"],"award-info":[{"award-number":["DEL-22-012"]}],"id":[{"id":"10.13039\/100031746","id-type":"DOI","asserted-by":"crossref"}]},{"award":["DEL-22-012"],"award-info":[{"award-number":["DEL-22-012"]}],"id":[{"id":"https:\/\/ror.org\/010cbts85","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/029chgv08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"UK Foreign, Commonwealth & Development Office"},{"id":[{"id":"https:\/\/ror.org\/037wke960","id-type":"ROR","asserted-by":"publisher"}]},{"name":"ELMA Relief Foundation"},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Disease","doi-asserted-by":"crossref","award":["5R01AI123422"],"award-info":[{"award-number":["5R01AI123422"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"award":["5R01AI123422"],"award-info":[{"award-number":["5R01AI123422"]}],"id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>During Zambia\u2019s 2023\u20132024 cholera outbreak, reliance on single-pathogen diagnostics risked overlooking co-circulating enteric pathogens. This study estimated the prevalence of rotavirus and described co-detected enteropathogens and rotavirus genotypes among patients admitted with suspected cholera. A sub-analysis was conducted on diarrhoeal stool specimens collected from patients who met the syndromic suspected cholera case definition. Samples were tested using the Bosphore\u00ae Gastroenteritis Panel v2, a multiplex PCR enteric panel, to detect rotavirus and other gastrointestinal pathogens. Rotavirus-positive specimens with sufficient viral load were further genotyped by RT-PCR targeting of the VP7 and VP4 genes. Among 319 suspected cholera admissions, rotavirus was detected in 18 patients (5.6%; 95% CI 3.4\u20138.8%), predominantly in children aged &lt;5 years (27.8%, 5\/18) and 6\u201317 years (27.8%, 5\/18). Co-infection was common, with 17\/18 (94.4%) of rotavirus-positive samples showing co-infection with at least one additional enteric pathogen, most frequently Campylobacter. Genotyping was successful in five samples and revealed heterogenous circulating strains, including G1P[8], G2P[4], G3P[6], G12P[6], and G1P[6]. Rotavirus accounted for a modest proportion of suspected cholera admissions and was frequently detected in mixed enteric infections, underscoring the value of multi-pathogen diagnostics and continued molecular surveillance during outbreak response.<\/jats:p>","DOI":"10.3390\/v18050508","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:43:51Z","timestamp":1777448631000},"page":"508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rotavirus Prevalence, Genetic Diversity, and Co-Infections During the 2023\u20132024 Cholera Outbreak in Zambia: Insights from Multi-Pathogen Diagnostics"],"prefix":"10.3390","volume":"18","author":[{"given":"Adriace","family":"Chauwa","sequence":"first","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Bosomprah","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"},{"name":"Department of Biostatistics, School of Public Health, University of Ghana, Accra P.O. Box LG13, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Phiri","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6217-0289","authenticated-orcid":false,"given":"Natasha M.","family":"Laban","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7813-8881","authenticated-orcid":false,"given":"Dhvani H.","family":"Kuntawala","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6338-4841","authenticated-orcid":false,"given":"Dennis","family":"Ngosa","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harriet","family":"Ng\u2019ombe","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"},{"name":"Centre for Epidemic Response and Innovation, Stellenbosch University, Private Bag X1, Stellenbosch 7599, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fraser","family":"Liswaniso","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaluma C.","family":"Luchen","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"},{"name":"Department of Global Health, Amsterdam Institute for Global Health and Development, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3163-712X","authenticated-orcid":false,"given":"Mutinta","family":"Muchimba","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Innocent","family":"Mwape","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"},{"name":"Zambia National Public Health Institute Reference Laboratory, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertha T.","family":"Nzangwa","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4164-8060","authenticated-orcid":false,"given":"Sekayi F.","family":"Tigere","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9539-4756","authenticated-orcid":false,"given":"Kennedy","family":"Chibesa","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8038-7107","authenticated-orcid":false,"given":"Suwilanji","family":"Silwamba","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7348-2835","authenticated-orcid":false,"given":"Michelo","family":"Simuyandi","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3309-0538","authenticated-orcid":false,"given":"Nyuma","family":"Mbewe","sequence":"additional","affiliation":[{"name":"Zambia National Public Health Institute Reference Laboratory, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0221-9527","authenticated-orcid":false,"given":"Roma","family":"Chilengi","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"},{"name":"Zambia National Public Health Institute Reference Laboratory, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7850-7915","authenticated-orcid":false,"given":"Caroline","family":"Chisenga","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Research in Zambia, P.O. Box 34681, Lusaka 10101, Zambia"},{"name":"Parasites and Microbes Programme, Wellcome Sanger Institute, Cambridge CB10 1SA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Thystrup, C., Majowicz, S.E., Kitila, D.B., Desta, B.N., Fayemi, O.E., Ayolabi, C.I., Hugho, E., Buys, E.M., Akanni, G.B., and Machava, N.E. (2024). Etiology-Specific Incidence and Mortality of Diarrheal Diseases in the African Region: A Systematic Review and Meta-Analysis. 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