{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:36:12Z","timestamp":1777869372627,"version":"3.51.4"},"reference-count":20,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Prevention and Control of Emerging and Major Infectious Diseases-National Science and Technology Major Project","award":["2025ZD01900100"],"award-info":[{"award-number":["2025ZD01900100"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Bovine adenovirus (BAdV) is associated with respiratory and enteric diseases in cattle. In this study, the complete genomic sequence of a novel BAdV strain (named BAdV\/LN\/CHN\/2023) was sequenced and annotated using the next-generation sequencing (NGS) technology. The viral genome comprises 32,391 base pairs with a GC content of 44.93%, encoding 33 predicted open reading frames (ORFs), consistent with the genomic organization of mastadenoviruses. Comparative genomic analysis confirmed that BAdV\/LN\/CHN\/2023 contains conserved structural and functional motifs characteristic of the genus Mastadenovirus. Phylogenetic analysis revealed that BAdV\/LN\/CHN\/2023 shares low similarity with all currently recognized bovine mastadenoviruses classified by the International Committee on Taxonomy of Viruses (ICTV). In addition, an open reading frame (ORF) encoding the 146R protein was annotated in this strain; this feature has not been identified in any previously recognized bovine mastadenoviruses. This study presents the first full-length genomic sequence of a putative BAdV-11 strain, and based on ICTV criteria, we propose that this strain represents a novel mastadenovirus species, supported by phylogenetic distance and genomic divergence. Our findings expand the known genetic diversity of BAdVs and contribute to a better understanding of their evolutionary relationships.<\/jats:p>","DOI":"10.3390\/v18050522","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:32:21Z","timestamp":1777555941000},"page":"522","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Characterization of a Novel Bovine Adenovirus Which Represents a Distinct Evolutionary Branch"],"prefix":"10.3390","volume":"18","author":[{"given":"Jinyu","family":"Sui","sequence":"first","affiliation":[{"name":"China Animal Health and Epidemiology Center, Qingdao 266032, China"},{"name":"Key Laboratory of Animal Biosafty Risk Prevention and Control (South), Ministry of Agriculture and Rural Affairs, Qingdao 266032, China"},{"name":"Key Laboratory of Animal Biosafty Risk Prevention, Qingdao 266032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suchun","family":"Wang","sequence":"additional","affiliation":[{"name":"China Animal Health and Epidemiology Center, Qingdao 266032, China"},{"name":"Key Laboratory of Animal Biosafty Risk Prevention and Control (South), Ministry of Agriculture and Rural Affairs, Qingdao 266032, China"},{"name":"Key Laboratory of Animal Biosafty Risk Prevention, Qingdao 266032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihao","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaicheng","family":"Wang","sequence":"additional","affiliation":[{"name":"China Animal Health and Epidemiology Center, Qingdao 266032, China"},{"name":"Key Laboratory of Animal Biosafty Risk Prevention and Control (South), Ministry of Agriculture and Rural Affairs, Qingdao 266032, China"},{"name":"Key Laboratory of Animal Biosafty Risk Prevention, Qingdao 266032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1128\/CMR.00052-07","article-title":"Adenoviruses in immunocompromised hosts","volume":"21","author":"Echavarria","year":"2008","journal-title":"Clin. 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