{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:23:45Z","timestamp":1790270625564,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MRC Discovery Medicine North (DiMeN) Doctoral Training Partnership","award":["MR\/W006944\/1"],"award-info":[{"award-number":["MR\/W006944\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Mosquito-borne diseases are a major global health concern, infecting up to 700 million people annually and causing over one million deaths. Of the several genera of biting mosquitoes, species of Anopheles are mostly studied for their ability (vector competence) to transmit Plasmodium protozoan parasites, some species of which cause malaria. More than 480 species of Anopheles have been described worldwide, and about 70 of these are responsible for Plasmodium spp. transmission. However, the focus on Anopheles as vectors of Plasmodium has led to a relative lack of study about the ability of Anopheles to transmit viruses. Some Anopheles species have been experimentally confirmed as competent for various arboviruses. In most cases, they are secondary vectors, with relatively low competence, contributing to overall transmission while other species of mosquito or other vectors are responsible for sustained transmission. Although secondary vectors may contribute less to transmission, they may play important epidemiological roles by extending transmission seasons and\/or providing a means of overwintering viruses. Here, we conducted a review of scientific repositories to build a database of known Anopheles competence for arboviruses. After exclusions, we retained 427 laboratory-confirmed studies from 7343 papers reviewed. Our analysis suggests some Anopheles spp. could contribute to arbovirus overwintering in temperate regions.<\/jats:p>","DOI":"10.3390\/v18050541","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T20:11:13Z","timestamp":1778271073000},"page":"541","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating the Role of Anopheles Mosquitoes in the Global Spread of Arboviruses: A Review of Laboratory-Confirmed Viral Competence"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0430-0417","authenticated-orcid":false,"given":"Rosheen Sungeni","family":"Mthawanji","sequence":"first","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Baylis","sequence":"additional","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Wardeh","sequence":"additional","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZB, UK"},{"name":"Department of Computer Science, University of Liverpool, Ashton Building, Liverpool L69 3DR, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7510-167X","authenticated-orcid":false,"given":"Marcus S. C.","family":"Blagrove","sequence":"additional","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Goddard, J. (2018). Mosquito-Borne Diseases. Infectious Diseases and Arthropods, Humana Press. [3rd ed.].","DOI":"10.1007\/978-3-319-75874-9_3"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.actatropica.2018.11.010","article-title":"Molecular epidemiology of dengue, yellow fever, Zika and Chikungunya arboviruses: An update","volume":"190","author":"Higuera","year":"2019","journal-title":"Acta Trop."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Jones, R., Kulkarni, M.A., Davidson, T.M.V., Team, R.L.R., and Talbot, B. (2020). Arbovirus vectors of epidemiological concern in the Americas: A scoping review of entomological studies on Zika, dengue and Chikungunya virus vectors. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0220753"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Gianchecchi, E., Cianchi, V., Torelli, A., and Montomoli, E. (2022). Yellow Fever: Origin, Epidemiology, Preventive Strategies and Future Prospects. Vaccines, 10.","DOI":"10.3390\/vaccines10030372"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/MMBR.64.1.115-137.2000","article-title":"Genetics of Mosquito Vector Competence","volume":"64","author":"Beerntsen","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Diaz, L.A., Flores, F.S., Quaglia, A., and Contigiani, M.S. (2013). Intertwined arbovirus transmission activity: Reassessing the transmission cycle paradigm. Front. Physiol., 3.","DOI":"10.3389\/fphys.2012.00493"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"30452","DOI":"10.2807\/1560-7917.ES.2017.22.4.30452","article-title":"Widespread activity of multiple lineages of Usutu virus, western Europe, 2016","volume":"22","author":"Cadar","year":"2017","journal-title":"Eurosurveillance"},{"key":"ref_8","first-page":"681","article-title":"Vertical transmission of arboviruses in mosquitoes: A historical perspective","volume":"28","author":"Lequime","year":"2014","journal-title":"Infect. Genet. Evol. J. Mol. Epidemiol. Evol. Genet. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"559","DOI":"10.4269\/ajtmh.2012.11-0654","article-title":"Detection of Persistent West Nile Virus RNA in Experimentally and Naturally Infected Avian Hosts","volume":"87","author":"Wheeler","year":"2012","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/1475-2875-10-151","article-title":"The contribution of aestivating mosquitoes to the persistence of Anopheles gambiae in the Sahel","volume":"10","author":"Adamou","year":"2011","journal-title":"Malar. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1093\/aesa\/63.1.210","article-title":"Physiological Condition of Overwintering Female Anopheles freeborni in California (Diptera: Culicidae)","volume":"63","author":"Washino","year":"1970","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1093\/jme\/tjad049","article-title":"Host-seeking activity of adult Culicoides sonorensis (Diptera: Ceratopogonidae) during winter in southern California, USA, and assessment of bluetongue virus overwintering","volume":"60","author":"Zhang","year":"2023","journal-title":"J. Med. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"407","DOI":"10.4269\/ajtmh.1996.55.407","article-title":"Facilitation of Rift Valley fever virus transmission by Plasmodium berghei sporozoites in Anopheles stephensi mosquitoes","volume":"55","author":"Vaughan","year":"1996","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1186\/s13071-023-05725-0","article-title":"Vector competence of Anopheles stephensi for O\u2019nyong-nyong virus: A risk for global virus spread","volume":"16","author":"Mutsaers","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_15","first-page":"459","article-title":"A Structured review on the Viruses of Anopheles Mosquitoes: The Potential Importance for Public Health","volume":"8","author":"Correa","year":"2023","journal-title":"Trop. Med. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Cottis, S., Blisnick, A.A., Failloux, A.B., and Vernick, K.D. (2023). Determinants of Chikungunya and O\u2019nyong-Nyong Virus Specificity for Infection of Aedes and Anopheles Mosquito Vectors. Viruses, 15.","DOI":"10.3390\/v15030589"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/s13071-023-05713-4","article-title":"Evaluating the mosquito host range of Getah virus and the vector competence of selected medically important mosquitoes in Getah virus transmission","volume":"16","author":"Azerigyik","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1089\/vbz.2008.0037","article-title":"Vector competence of Australian mosquito species for a North American strain of West Nile virus","volume":"8","author":"Jansen","year":"2008","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"375","DOI":"10.2217\/fvl-2019-0051","article-title":"Mayaro virus: A neglected threat could cause the next worldwide viral epidemic","volume":"14","author":"Avilla","year":"2019","journal-title":"Future Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"412","DOI":"10.4269\/ajtmh.22-0417","article-title":"Anopheles albimanus is a Potential Alphavirus Vector in the Americas","volume":"108","author":"Terradas","year":"2023","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Brustolin, M., Pujhari, S., Henderson, C.A., and Rasgon, J.L. (2018). Anopheles mosquitoes may drive invasion and transmission of Mayaro virus across geographically diverse regions. PLoS Negl. Trop. Dis., 12.","DOI":"10.1101\/359349"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"303","DOI":"10.3201\/eid2802.203810","article-title":"Role of Anopheles Mosquitoes in Cache Valley Virus Lineage Displacement, New York, USA","volume":"28","author":"Dieme","year":"2022","journal-title":"Emerg. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"7Gaye, A., Diagne, M.M., Ndiaye, E.H., Dior Ndione, M.H., Faye, M., Talla, C., Fall, G., Ba, Y., Diallo, D., and Dia, I. (2020). Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal. Emerg. Microbes Infect., 9, 496\u2013504.","DOI":"10.1080\/22221751.2020.1730710"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1186\/s13071-022-05342-3","article-title":"Vector competence of Anopheles quadrimaculatus and Aedes albopictus for genetically distinct Jamestown Canyon virus strains circulating in the Northeast United States","volume":"15","author":"Dieme","year":"2022","journal-title":"Parasit. Vectors"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e3096","DOI":"10.7717\/peerj.3096","article-title":"Vector competence of Anopheles and Culex mosquitoes for Zika virus","volume":"5","author":"Dodson","year":"2017","journal-title":"PeerJ"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"136","DOI":"10.4269\/ajtmh.1996.54.136","article-title":"Vector competence of Egyptian mosquitoes for Rift Valley fever virus","volume":"54","author":"Turell","year":"1996","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1186\/s13071-020-04478-4","article-title":"Transmission potential of Mayaro virus by Aedes albopictus, and Anopheles quadrimaculatus from the USA","volume":"13","author":"Dieme","year":"2020","journal-title":"Parasit. Vectors"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1093\/jmedent\/34.3.346","article-title":"Vector competence of mosquitoes (Diptera: Culicidae) from Massachusetts for a sympatric isolate of eastern equine encephalomyelitis virus","volume":"34","author":"Vaidyanathan","year":"1997","journal-title":"J. Med. Entomol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/mve.12291","article-title":"Vector competence of Culex antennatus and Anopheles coustani mosquitoes for Rift Valley fever virus in Madagascar","volume":"32","author":"Nepomichene","year":"2018","journal-title":"Med. Vet. Entomol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Drouin, A., Chevalier, V., Durand, B., and Balenghien, T. (2022). Vector Competence of Mediterranean Mosquitoes for Rift Valley Fever Virus: A Meta-Analysis. Pathogens, 11.","DOI":"10.3390\/pathogens11050503"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4269\/ajtmh.1988.38.440","article-title":"Vector potential of selected North American mosquito species for Rift Valley fever virus","volume":"38","author":"Gargan","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1603\/ME13049","article-title":"Potential for mosquitoes (Diptera: Culicidae) from Florida to transmit Rift Valley fever virus","volume":"50","author":"Turell","year":"2013","journal-title":"J. Med. Entomol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/jmedent\/47.5.884","article-title":"Potential for North American mosquitoes (Diptera: Culicidae) to transmit rift valley fever virus","volume":"47","author":"Turell","year":"2010","journal-title":"J. Med. Entomol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e4324","DOI":"10.7717\/peerj.4324","article-title":"Vector competence of selected North American Anopheles and Culex mosquitoes for Zika virus","volume":"6","author":"Dodson","year":"2018","journal-title":"PeerJ"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1099\/vir.0.060764-0","article-title":"Sunguru virus: A novel virus in the family Rhabdoviridae isolated from a chicken in north-western Uganda","volume":"95","author":"Ledermann","year":"2014","journal-title":"J. Gen. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1603\/ME14063","article-title":"Vector Competence of Selected Mosquito Species in Kenya for Ngari and Bunyamwera Viruses","volume":"51","author":"Odhiambo","year":"2014","journal-title":"J. Med. Entomol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1093\/jmedent\/35.5.660","article-title":"Role of Anopheles quadrimaculatus and Coquillettidia perturbans (Diptera: Culicidae) in the Transmission Cycle of Cache Valley Virus (Bunyaviridae: Bunyavirus) in the Midwest, U.S.A","volume":"35","author":"Blackmore","year":"1998","journal-title":"J. Med. Entomol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Martinet, J.P., Bohers, C., Vazeille, M., Fert\u00e9, H., Mousson, L., Mathieu, B., Depaquit, J., and Failloux, A.-B. (2023). Assessing vector competence of mosquitoes from northeastern France to West Nile virus and Usutu virus. PLoS Neglected Trop. Dis., 17.","DOI":"10.1101\/2023.02.07.527438"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1186\/s13071-020-04072-8","article-title":"Anopheles gambiae densovirus (AgDNV) negatively affects Mayaro virus infection in Anopheles gambiae cells and mosquitoes","volume":"13","author":"Urakova","year":"2020","journal-title":"Parasit. Vectors"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Van den Eynde, C., Sohier, C., Matthijs, S., and De Regge, N. (2023). Belgian Anopheles plumbeus Mosquitoes Are Competent for Japanese Encephalitis Virus and Readily Feed on Pigs, Suggesting a High Vectorial Capacity. Microorganisms, 11.","DOI":"10.3390\/microorganisms11061386"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"616","DOI":"10.4269\/ajtmh.2005.72.616","article-title":"Differential infectivities of o\u2019nyong-nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes","volume":"72","author":"Vanlandingham","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1603\/0022-2585-37.5.701","article-title":"Effect of Eastern Equine Encephalomyelitis Virus on the Survival of Aedes albopictus, Anopheles quadrimaculatus, and Coquillettidia perturbans (Diptera: Culicidae)","volume":"37","author":"Moncayo","year":"2000","journal-title":"J. Med. Entomol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e0196022","DOI":"10.1128\/jvi.01960-22","article-title":"Culex tarsalis Is a Competent Host of the Insect-Specific Alphavirus Eilat Virus (EILV)","volume":"97","author":"Joseph","year":"2023","journal-title":"J. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"603","DOI":"10.4269\/ajtmh.1985.34.603","article-title":"Comparative susceptibility of mosquito species and strains to oral and parenteral infection with dengue and Japanese encephalitis viruses","volume":"34","author":"Rosen","year":"1985","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1111\/j.0962-1075.2004.00521.x","article-title":"Infection patterns of o\u2019nyong nyong virus in the malaria-transmitting mosquito, Anopheles gambiae","volume":"13","author":"Brault","year":"2004","journal-title":"Insect Mol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1186\/s13071-014-0595-2","article-title":"Eilat virus displays a narrow mosquito vector range","volume":"7","author":"Nasar","year":"2014","journal-title":"Parasit. Vectors"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Nanfack Minkeu, F., and Vernick, K.D. (2018). A Structured review of the Natural Virome of Anopheles Mosquitoes. Viruses, 10.","DOI":"10.3390\/v10050222"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1475-2875-12-56","article-title":"Successful malaria elimination strategies require interventions that target changing vector behaviours","volume":"12","author":"Russell","year":"2013","journal-title":"Malar. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.meegid.2017.11.003","article-title":"West Nile virus, Anopheles flavivirus, a novel flavivirus as well as Merida-like rhabdovirus Turkey in field-collected mosquitoes from Thrace and Anatolia","volume":"57","author":"Brinkmann","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.trstmh.2008.03.011","article-title":"Potential mosquito vectors of arboviruses in Portugal: Species, distribution, abundance and West Nile infection","volume":"102","author":"Almeida","year":"2008","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Seufi, A.M., and Galal, F.H. (2010). Role of Culex and Anopheles mosquito species as potential vectors of rift valley fever virus in Sudan outbreak, 2007. BMC Infect. Dis., 10.","DOI":"10.1186\/1471-2334-10-65"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1146\/annurev-ento-011613-162023","article-title":"Mosquito Diapause","volume":"59","author":"Denlinger","year":"2014","journal-title":"Annu. Rev. Entomol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1186\/s13071-024-06620-y","article-title":"Overwintering of Usutu virus in mosquitoes, The Netherlands","volume":"17","author":"Koenraadt","year":"2024","journal-title":"Parasit. Vectors"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Simonin, Y. (2024). Circulation of West Nile Virus and Usutu Virus in Europe: Overview and Challenges. Viruses, 16.","DOI":"10.3390\/v16040599"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1186\/s13071-024-06367-6","article-title":"Overwintering West Nile virus in active Culex pipiens mosquito populations in Greece","volume":"17","author":"Balatsos","year":"2024","journal-title":"Parasit. Vectors"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/5\/541\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T20:13:05Z","timestamp":1778271185000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/5\/541"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,8]]},"references-count":55,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["v18050541"],"URL":"https:\/\/doi.org\/10.3390\/v18050541","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,8]]}}}