{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:10:52Z","timestamp":1792026652681,"version":"4.4.0"},"reference-count":39,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n\/Ministerio de Ciencia, Innovaci\u00f3n y Universidades, Spain","doi-asserted-by":"publisher","award":["JDC2022-048632-I"],"award-info":[{"award-number":["JDC2022-048632-I"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"award":["JDC2022-048632-I"],"award-info":[{"award-number":["JDC2022-048632-I"]}],"id":[{"id":"https:\/\/ror.org\/05r0vyz12","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["304106-22"],"award-info":[{"award-number":["304106-22"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Emerging and re-emerging pathogens have been increasingly investigated in cetaceans worldwide, particularly in odontocetes, whereas infectious diseases in mysticetes remain poorly understood. This study surveyed herpesvirus in 51 baleen whales stranded along the Brazilian coast, between 2008 and 2022: humpback whales (Megaptera novaeangliae, n = 46), Bryde\u2019s whales (Balaenoptera brydei, n = 3), a southern right whale (Eubalaena australis, n = 1), and a common minke whale (Balaenoptera acutorostrata, n = 1). A total of 159 tissue samples were analyzed using nested PCR assays targeting the herpesviral DNA polymerase (DPOL) and glycoprotein B (gB) genes. Herpesvirus DNA was detected in 25.5% (13\/51) of individuals, including 23.9% (11\/46) of humpback whales, one Bryde\u2019s whale, and one common minke whale. Positive animals included six calves, six juveniles, and one adult. All 13 cases were detected by DPOL PCR, yielding eight sequence types (five alphaherpesviruses and three gammaherpesviruses). Additionally, six of them were also positive for gB, identifying four gammaherpesvirus sequence types within the genus Rhadinovirus. Humpback whales showed both alpha- and gammaherpesviruses, including one coinfection, whereas gammaherpesviruses were detected in Bryde\u2019s and minke whales. This study reports the first herpesvirus in Bryde\u2019s whales and expands the geographic range of infection in minke whales. The high prevalence in calves suggests vertical transmission.<\/jats:p>","DOI":"10.3390\/v18060612","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:31:56Z","timestamp":1779885116000},"page":"612","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Herpesvirus Diversity in Stranded Mysticetes of Brazil, Southwestern Atlantic"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7162-2371","authenticated-orcid":false,"given":"Vanessa","family":"Dal Bianco","sequence":"first","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2999-3395","authenticated-orcid":false,"given":"Jos\u00e9 Luiz","family":"Cat\u00e3o-Dias","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Zamana-Ramblas","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-1870","authenticated-orcid":false,"given":"Samira","family":"Costa-Silva","sequence":"additional","affiliation":[{"name":"Instituto Baleia Jubarte, Caravelas 45900-000, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina","family":"Ewbank","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA), CSIC, 28130 Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara Sophia","family":"Codeas","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Castaldo Colosio","sequence":"additional","affiliation":[{"name":"Instituto Baleia Jubarte, Caravelas 45900-000, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3521-1409","authenticated-orcid":false,"given":"Marta J.","family":"Cremer","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ecologia e Conserva\u00e7\u00e3o de Tetr\u00e1podes Marinhos e Costeiros\u2014TETRAMAR, Universidade da Regi\u00e3o de Joinville, Joinville 89240-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenyffer V.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ecologia e Conserva\u00e7\u00e3o de Tetr\u00e1podes Marinhos e Costeiros\u2014TETRAMAR, Universidade da Regi\u00e3o de Joinville, Joinville 89240-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9701-8236","authenticated-orcid":false,"given":"Giulia G.","family":"Lemos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ecologia e Conserva\u00e7\u00e3o de Tetr\u00e1podes Marinhos e Costeiros\u2014TETRAMAR, Universidade da Regi\u00e3o de Joinville, Joinville 89240-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6111-6301","authenticated-orcid":false,"given":"Carlos","family":"Sacrist\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA), CSIC, 28130 Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aricia","family":"Duarte-Benvenuto","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"ref_1","unstructured":"Lodi, L., and Borobia, M. (2013). Baleias, Botos e Golfinhos do Brasil: Guia de Identifica\u00e7\u00e3o, Technica Books Editora."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1644\/07-MAMM-S-312R1.1","article-title":"Marine mammals as ecosystem sentinels","volume":"89","author":"Moore","year":"2008","journal-title":"J. Mammal."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1177\/0300985810388525","article-title":"Marine mammals as sentinel species for oceans and human health","volume":"48","author":"Bossart","year":"2011","journal-title":"Vet. Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"411","DOI":"10.4257\/oeco.2013.1703.09","article-title":"Exposi\u00e7\u00e3o de cet\u00e1ceos a compostos butilest\u00e2nicos: Uma revis\u00e3o","volume":"17","author":"Schilithz","year":"2013","journal-title":"Oecol. Aust."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1111\/mms.12241","article-title":"Habitat use by southern right whales (Eubalaena australis (Desmoulins, 1822)) in their main northernmost calving area in the western South Atlantic","volume":"31","author":"Seyboth","year":"2015","journal-title":"Mar. Mammal Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1590\/1678-4685-gmb-2017-0052","article-title":"Effective population size and the genetic consequences of commercial whaling on the humpback whales (Megaptera novaeangliae) from the Southwestern Atlantic Ocean","volume":"41","author":"Engel","year":"2018","journal-title":"Genet. Mol. Biol."},{"key":"ref_7","unstructured":"International Whaling Commission (2026, January 23). IWC Regulations and Guidelines. Available online: https:\/\/iwc.int."},{"key":"ref_8","unstructured":"Barreto, R.K.M. (2012). Hist\u00f3rico da Ca\u00e7a a Cet\u00e1ceos da Superfam\u00edlia Mysticeti no Brasil e no Mundo. [Bachelor\u2019s Thesis, Universidade Federal do Rio Grande do Sul]. Available online: http:\/\/hdl.handle.net\/10183\/72379."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/tbed.12781","article-title":"Paracoccidioidomycosis ceti in an Atlantic bottlenose dolphin (Tursiops truncatus), Brazil","volume":"65","author":"Ewbank","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Groch, K.R., Diaz-Delgado, J., Marcondes, M.C.C., Colosio, A.C., Santos-Neto, E.B., Carvalho, V.L., Boos, G.S., de Meirelles, A.C.O., Ramos, H.G.d.C., and Guimar\u00e3es, J.P. (2018). Pathology and causes of death in stranded humpback whales (Megaptera novaeangliae) from Brazil. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194872"},{"key":"ref_11","first-page":"1674","article-title":"Molecular, serological, pathological, immunohistochemical and microbiological investigation of Brucella spp. in marine mammals of Brazil reveals new cetacean hosts","volume":"66","author":"Carvalho","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"214","DOI":"10.3201\/eid2901.221549","article-title":"Short-finned pilot whale strandings associated with pilot whale morbillivirus, Brazil","volume":"29","author":"Soares","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0147-9571(02)00036-X","article-title":"Pathological and serological evidence of Brucella infection in baleen whales (Mysticeti) in the western North Pacific","volume":"26","author":"Ohishi","year":"2003","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3354\/dao03207","article-title":"Pulmonary and systemic fungal infections in an Atlantic spotted dolphin and a Bryde\u2019s whale, Brazil","volume":"128","author":"Groch","year":"2018","journal-title":"Dis. Aquat. Org."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3354\/dao03293","article-title":"Omphalitis, urachocystitis and septicemia by Streptococcus dysgalactiae in a southern right whale calf (Eubalaena australis), Brazil","volume":"131","author":"Bianchi","year":"2018","journal-title":"Dis. Aquat. Org."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00705-008-0278-4","article-title":"The order Herpesvirales","volume":"154","author":"Davison","year":"2009","journal-title":"Arch. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.actatropica.2018.11.021","article-title":"Novel herpesviruses in riverine and marine cetaceans from South America","volume":"190","author":"Ewbank","year":"2019","journal-title":"Acta Trop."},{"key":"ref_18","first-page":"2403","article-title":"Highly divergent herpesviruses in threatened river dolphins from Brazil","volume":"11","author":"Novoselecki","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"35S","DOI":"10.1016\/S0002-9343(98)00160-0","article-title":"Stress-associated immune modulation: Relevance to viral infections and chronic fatigue syndrome","volume":"105","author":"Glaser","year":"1998","journal-title":"Am. J. Med."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Arbelo, M., Belli\u00e8re, E.N., Sierra, E., Sacchini, S., Esper\u00f3n, F., Andrada, M., Rivero, M., Diaz-Delgado, J., and Fern\u00e1ndez, A. (2012). Herpesvirus infection associated with interstitial nephritis in a beaked whale (Mesoplodon densirostris). BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-243"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Sierra, E., Fern\u00e1ndez, A., Fern\u00e1ndez-Maldonado, C., Sacchini, S., Felipe-Jim\u00e9nez, I., Segura-G\u00f6thlin, S., Colom-Rivero, A., C\u00e2mara, N., Puig-Lozano, R., and Rambaldi, A.M. (2022). Molecular characterization of herpesviral encephalitis in cetaceans: Correlation with histopathological and immunohistochemical findings. Animals, 12.","DOI":"10.3390\/ani12091149"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Melero, M., Crespo-Picazo, J.L., Rubio-Guerri, C., Garc\u00eda-P\u00e1rraga, D., and S\u00e1nchez-Vizca\u00edno, J.M. (2015). First molecular determination of herpesvirus from two mysticete species stranded in the Mediterranean Sea. BMC Vet. Res., 11.","DOI":"10.1186\/s12917-015-0596-1"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3354\/dao03590","article-title":"Meningoencephalitis in a common minke whale (Balaenoptera acutorostrata) associated with Brucella pinnipedialis and gamma-herpesvirus infection","volume":"144","author":"Davison","year":"2021","journal-title":"Dis. Aquat. Org."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.1038\/s41598-023-45315-9","article-title":"Survey of selected viral agents (herpesvirus, adenovirus and hepatitis E virus) in liver and lung samples of cetaceans, Brazil","volume":"14","author":"Ewbank","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10344-024-01782-7","article-title":"Molecular detection of herpesvirus in a skin lesion of a humpback whale (Megaptera novaeangliae) from the Western Mediterranean Sea","volume":"70","year":"2024","journal-title":"Eur. J. Wildl. Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sekine, W., Kawasaki, J., Ohira, K., Li, K., Katayama, M., Ichikawa, A., Wakabayashi, Y., Takenaka-Uema, A., Murakami, S., and Horimoto, T. (2025). First detection of an alphaherpesvirus gene in humpback whale blow samples collected non-invasively using unmanned aerial vehicles. Viruses, 17.","DOI":"10.3390\/v17111411"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Costa, H., Ramstedt, P., Bergsma, M., Jourdain, E., Morange, Z., Bl\u00e9vin, P., Basran, C.J., Rasmussen, M.H., Dawson, T.P., and Dawson, H.Y. (2025). Deep breath out: Molecular survey of selected pathogens in blow and skin biopsies from North Atlantic cetaceans. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-05152-6"},{"key":"ref_28","unstructured":"Geraci, J.R., and Lounsbury, V.J. (2026, January 28). Marine Mammals Ashore: A Field Guide for Strandings, Available online: https:\/\/repository.library.noaa.gov\/view\/noaa\/39015."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e01468","DOI":"10.1002\/ecs2.1468","article-title":"Noninvasive unmanned aerial vehicle provides estimates of the energetic cost of reproduction in humpback whales","volume":"7","author":"Christiansen","year":"2016","journal-title":"Ecosphere"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1128\/jcm.34.7.1666-1671.1996","article-title":"Detection and analysis of diverse herpesviral species by consensus primer PCR","volume":"34","author":"VanDevanter","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1128\/JVI.02646-07","article-title":"Novel mammalian herpesviruses and lineages within the Gammaherpesvirinae: Cospeciation and interspecies transfer","volume":"82","author":"Ehlers","year":"2008","journal-title":"J. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1093\/bioinformatics\/btr088","article-title":"ProtTest 3: Fast selection of best-fit models of protein evolution","volume":"27","author":"Darriba","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Vargas-Castro, I., Melero, M., Crespo-Picazo, J.L., Jim\u00e9nez, M.L.\u00c1., Sierra, E., Rubio-Guerri, C., Arbelo, M., Fern\u00e1ndez, A., Garc\u00eda-P\u00e1rraga, D., and S\u00e1nchez-Vizca\u00edno, J.M. (2021). Systematic determination of herpesvirus in free-ranging cetaceans stranded in the Western Mediterranean: Tissue tropism and associated lesions. Viruses, 13.","DOI":"10.3390\/v13112180"},{"key":"ref_35","first-page":"222","article-title":"Herpesvirus infection in marine mammals: A retrospective molecular survey of stranded cetaceans in the Portuguese coastline","volume":"67","author":"Bento","year":"2019","journal-title":"Infect. Genet. Evol. J. Mol. Epidemiol. Evol. Genet. Infect. Dis."},{"key":"ref_36","unstructured":"Petrobras (2026, January 23). SIMBA\u2014Sistema de Monitoramento da Biota Aqu\u00e1tica. Available online: https:\/\/simba.petrobras.com.br."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.jpeds.2004.06.017","article-title":"Congenital infections with human herpesvirus 6 (HHV6) and human herpesvirus 7 (HHV7)","volume":"145","author":"Hall","year":"2004","journal-title":"J. Pediatr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.micpath.2010.10.006","article-title":"Symptomless intrauterine transmission of bovine herpesvirus 4 to bovine fetuses","volume":"50","author":"Egyed","year":"2011","journal-title":"Microb. Pathog."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"79","DOI":"10.2746\/0425164044864732","article-title":"Equine herpesvirus-1 abortion: Atypical cases with lesions largely or wholly restricted to the placenta","volume":"36","author":"Smith","year":"2004","journal-title":"Equine Vet. J."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/6\/612\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:47:20Z","timestamp":1779886040000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/6\/612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,27]]},"references-count":39,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["v18060612"],"URL":"https:\/\/doi.org\/10.3390\/v18060612","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,27]]}}}