{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:53:55Z","timestamp":1785405235662,"version":"3.56.0"},"reference-count":52,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UNIVERSIDAD NACIONAL AUT\u00d3NOMA DE M\u00c9XICO","award":["PIAPI CI2465"],"award-info":[{"award-number":["PIAPI CI2465"]}]},{"award":["PIAPI CI2465"],"award-info":[{"award-number":["PIAPI CI2465"]}],"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["829352"],"award-info":[{"award-number":["829352"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]},{"award":["829352"],"award-info":[{"award-number":["829352"]}],"id":[{"id":"https:\/\/ror.org\/059ex5q34","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>PPV5 NS1 is a nonstructural and multifunctional protein comprising helicase and nuclease domains. The helicase domain contains conserved motifs from superfamily 3 helicases, including Walker A, Walker B, Motif B\u2019, Motif C, and Box VII, whereas the nuclease domain consists of glutamate, a HUH motif, lysine, and tyrosine. In Mexico, the reported prevalence of PPV5 is higher than in other countries, with notable amino acid differences compared with pathogenic PPVs. This study compares the helicase and nuclease domains from a full-length PPV5 NS1 sequence with porcine parvovirus 1 (PPV1) and canine parvovirus (CPV) to characterize the protein further and perform three-dimensional (3D) modeling using bioinformatic tools, including solvent-accessible surface area (SASA) and electrostatic potential assessments. The main findings highlight the ATP-binding pocket, showing electrostatic values in PPV5 that contrast with PPV1 and CPV. The electrostatic potential 3D models suggest those differences involve non-conserved regions. In particular, the PPV5 Box VII surface is predominantly negative due to a glutamate substitution at position 7. In the nuclease domain, the interaction with Mg2+ differs between PPV5 and pathogenic PPV. The electrostatic findings suggest that these differences may have functional implications for both domains, but confirmation must be completed with functional assays.<\/jats:p>","DOI":"10.3390\/v18060631","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:17:31Z","timestamp":1780467451000},"page":"631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization of Helicase and Nuclease Domains in PPV5 NS1 from Mexican Full-Length Sequence"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9886-1532","authenticated-orcid":false,"given":"Diana Michele","family":"Araiza-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Master\u2019s and Doctoral Program in Animal Production and Health Sciences, Cuautitl\u00e1n College of Superior Studies, National Autonomous University of Mexico (UNAM), Carretera Cuautitl\u00e1n-Teoloyucan Km 2.5, Cuautitl\u00e1n Izcalli 54714, Estado de M\u00e9xico, Mexico"},{"name":"Department of Biological Sciences, Cuautitl\u00e1n College of Superior Studies, National Autonomous University of Mexico (UNAM), Carretera Cuautitl\u00e1n-Teoloyucan Km 2.5, Cuautitl\u00e1n Izcalli 54714, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Vargas-Ruiz","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Cuautitl\u00e1n College of Superior Studies, National Autonomous University of Mexico (UNAM), Carretera Cuautitl\u00e1n-Teoloyucan Km 2.5, Cuautitl\u00e1n Izcalli 54714, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1475-8982","authenticated-orcid":false,"given":"Ernesto","family":"Mar\u00edn-Flamand","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Cuautitl\u00e1n College of Superior Studies, National Autonomous University of Mexico (UNAM), Carretera Cuautitl\u00e1n-Teoloyucan Km 2.5, Cuautitl\u00e1n Izcalli 54714, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7430-5657","authenticated-orcid":false,"given":"Rosa Elena","family":"Sarmiento-Silva","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Department of Microbiology and Immunology, College of Veterinary Medicine and Animal Science, National Autonomous University of Mexico (UNAM), Av. Universidad 3000, Coyoac\u00e1n, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Iv\u00e1n","family":"S\u00e1nchez-Betancourt","sequence":"additional","affiliation":[{"name":"Department of Swine Medicine and Animal Science, College of Veterinary Medicine and Animal Science, National Autonomous University of Mexico (UNAM), Av. Universidad 3000, Coyoac\u00e1n, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Omar","family":"Hern\u00e1ndez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Cuautitl\u00e1n College of Superior Studies, National Autonomous University of Mexico (UNAM), Carretera Cuautitl\u00e1n-Teoloyucan Km 2.5, Cuautitl\u00e1n Izcalli 54714, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda Ang\u00e9lica","family":"Garc\u00eda-Camacho","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Cuautitl\u00e1n College of Superior Studies, National Autonomous University of Mexico (UNAM), Carretera Cuautitl\u00e1n-Teoloyucan Km 2.5, Cuautitl\u00e1n Izcalli 54714, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1053\/jcpa.1998.0295","article-title":"Experimental Reproduction of Severe Wasting Disease by Co-Infection of Pigs with Porcine Circovirus and Porcine Parvovirus","volume":"121","author":"Allan","year":"1999","journal-title":"J. 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