{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:59:59Z","timestamp":1780639199944,"version":"3.54.1"},"reference-count":99,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013832","name":"Emerging Pathogens Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100013832","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Under a One Health framework, viruses of veterinary and zoonotic importance pose significant threats to animal and human health, food security, and livelihoods, particularly in regions with intense human\u2013animal interactions. In West Africa, despite recent advances in surveillance programs, important gaps remain in understanding viral diversity and cross-species transmission at wildlife\u2013livestock interfaces. We conducted metagenomic surveillance to characterize viruses circulating across livestock, domestic animals, and wildlife in rural Ghana in 165 animals sampled across five regions. Viral RNA from serum and tissue samples was sequenced with the Illumina platform, and genomes were de novo assembled with MEGAHIT. Phylogenetic relationships were reconstructed using Bayesian approaches. We report the first genomic sequences of porcine parvovirus 3, canine parvovirus, rotavirus A genotype R16, and bovine hepacivirus subtype B from Ghana in over a decade. Phylogenetic analyses revealed intercontinental linkages between Africa and Europe for parvoviruses, persistence of hepacivirus lineages, and evidence of cross-species transmission for rotavirus. Notably, detection in apparently healthy animals highlights underrecognized circulation, gaps in vaccination effectiveness, trade-related biosecurity vulnerabilities, and the role of wildlife in viral maintenance and transmission. Our findings reveal dynamic viral diversity and connectivity across animal populations and ecological interfaces, emphasizing the fluid and interconnected nature of pathogen circulation within One Health systems. By integrating metagenomics and phylogenetics, this study provides a scalable framework for enhancing surveillance capacity, enabling the early detection of emerging threats and informing targeted strategies to mitigate zoonotic and economically important viral diseases in West Africa.<\/jats:p>","DOI":"10.3390\/v18060644","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:08:07Z","timestamp":1780510087000},"page":"644","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["One Health Genomic Surveillance at Human\u2013Animal Interfaces in Rural Ghana Reveals Underreported Viruses of Zoonotic and Economic Concern"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0273-1128","authenticated-orcid":false,"given":"Julia E.","family":"Paoli","sequence":"first","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA"},{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32610, USA"},{"name":"Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"One Health Center of Excellence, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2106-1166","authenticated-orcid":false,"given":"N\u00eddia S.","family":"Trov\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA"},{"name":"Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA"},{"name":"National Center for Supercomputing Applications, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7092-7237","authenticated-orcid":false,"given":"Theophilus","family":"Odoom","sequence":"additional","affiliation":[{"name":"Accra Veterinary Laboratory, Veterinary Services Directorate, Accra P.O. Box M161, Ghana"},{"name":"School of Veterinary Medicine, College of Basic and Applied Sciences, University of Ghana, Legon, Accra P.O. Box LG139, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quaneeta","family":"Mohktar","sequence":"additional","affiliation":[{"name":"Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9367-224X","authenticated-orcid":false,"given":"Kwame Boamah","family":"Buabeng","sequence":"additional","affiliation":[{"name":"Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bright","family":"Adu","sequence":"additional","affiliation":[{"name":"Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2125-6382","authenticated-orcid":false,"given":"William","family":"Tasiame","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1509-691X","authenticated-orcid":false,"given":"Benita","family":"Anderson","sequence":"additional","affiliation":[{"name":"Veterinary Services Directorate, Ministry of Food and Agriculture, Accra P.O. Box M37, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Nana Yaw","family":"Tawiah-Yingar","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, College of Basic and Applied Sciences, University of Ghana, Legon, Accra P.O. Box LG139, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuttichantran","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA"},{"name":"Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2938-4549","authenticated-orcid":false,"given":"Michael E.","family":"von Fricken","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA"},{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32610, USA"},{"name":"One Health Center of Excellence, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6499-5946","authenticated-orcid":false,"given":"Gloria Ivy","family":"Mensah","sequence":"additional","affiliation":[{"name":"Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8957-3363","authenticated-orcid":false,"given":"Mario","family":"Mietzsch","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, College of Medicine, Center for Structural Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2792-935X","authenticated-orcid":false,"given":"Robert","family":"McKenna","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, College of Medicine, Center for Structural Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0829-5774","authenticated-orcid":false,"given":"Sherry Ama Mawuko","family":"Johnson","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, College of Basic and Applied Sciences, University of Ghana, Legon, Accra P.O. Box LG139, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3819-5520","authenticated-orcid":false,"given":"Carla N.","family":"Mavian","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA"},{"name":"Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"Centre for Epidemic Response and Innovation, School for Data Science and Computational Thinking, Stellenbosch University, Stellenbosch 7600, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gebreyes, W.A., Dupouy-Camet, J., Newport, M.J., Oliveira, C.J.B., Schlesinger, L.S., Saif, Y.M., Kariuki, S., Saif, L.J., Saville, W., and Wittum, T. (2014). 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