{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:45:14Z","timestamp":1782801914824,"version":"3.54.5"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T00:00:00Z","timestamp":1782518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institutional Funds of Istituto Superiore di Sanit\u00e0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>HEV genotype 3 is classified into several subtypes. In Italy, 3f, 3c, 3e and, rarely, 3a subtypes are usually detected in human hepatitis E cases: the present study documents, for the first time, the detection of the 3i subtype, previously described in Italy exclusively from wild boars. Routine surveillance by short Sanger sequences highlighted five hepatitis E cases with unusual HEV subtype between 2019 and 2023 in a small geographical area in Umbria, Central Italy. Further characterization of the whole HEV genome by NGS was successful in three of them. Through phylogenetic and p-distance analysis, all the sequences could be classified as subtype 3i, and three of them proved to be highly related to some strains observed in wild boars sampled in the same geographical area. The 3i subtype had never been detected previously in humans in Italy; it is likely that increased local circulation of 3i-like strains in wild boars may have increased the chance for their transmission to humans. Investigation of the possible risk factors for HEV infection highlighted consumption of raw\/undercooked products from wild boars\/pigs in three cases. However, in the remaining two cases, this source seems unlikely, and transmission might have occurred indirectly from contaminated sources, such as wastewater and home-grown vegetables. Further studies are needed to investigate if these latter sources may be more common than previously thought or may play a role in relation to specific HEV subtypes and\/or only in rare and exceptional circumstances.<\/jats:p>","DOI":"10.3390\/v18070709","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T05:58:04Z","timestamp":1782799084000},"page":"709","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Identification of HEV Subtype 3i in Human Hepatitis E Cases in Central Italy"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3035-5510","authenticated-orcid":false,"given":"Roberto","family":"Bruni","sequence":"first","affiliation":[{"name":"Unit of Viral Hepatitis and Oncovirus and Retrovirus Diseases, Department of Infectious Diseases, Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Equestre","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2335-3154","authenticated-orcid":false,"given":"Valentina","family":"Curini","sequence":"additional","affiliation":[{"name":"National Reference Center for Whole Genome Sequencing of Microbial Pathogens, Istituto Zooprofilattico Sperimentale Abruzzo-Molise \u201cG. 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Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1099\/jgv.0.000393","article-title":"Proposed reference sequences for hepatitis E virus subtypes","volume":"97","author":"Smith","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1053\/j.gastro.2015.10.048","article-title":"Chronic infection with camelid hepatitis E virus in a liver transplant recipient who regularly consumes camel meat and milk","volume":"150","author":"Lee","year":"2016","journal-title":"Gastroenterology"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Doceul, V., Bagdassarian, E., Demange, A., and Pavio, N. (2016). Zoonotic hepatitis E virus: Classification, animal reservoirs and transmission routes. Viruses, 8.","DOI":"10.3390\/v8100270"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/978-981-99-1304-6_4","article-title":"Hepatitis E as a zoonosis","volume":"Volume 1417","author":"Wang","year":"2023","journal-title":"Hepatitis E Virus"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1086\/655898","article-title":"Pig liver sausage as a source of hepatitis E virus transmission to humans","volume":"202","author":"Colson","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jcv.2016.06.010","article-title":"Hepatitis E virus: Assessment of the epidemiological situation in humans in Europe, 2014\/15","volume":"82","author":"Adlhoch","year":"2016","journal-title":"J. Clin. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1099\/jgv.0.001435","article-title":"Update: Proposed reference sequences for subtypes of hepatitis E virus (species Orthohepevirus A)","volume":"101","author":"Smith","year":"2020","journal-title":"J. Gen. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Dencs, \u00c1., Hettmann, A., Zsichla, L., M\u00fcller, V., D\u00f6m\u00f6t\u00f6r, A., Barna-L\u00e1z\u00e1r, \u00c1., Barcsay, E., and Tak\u00e1cs, M. (2025). Molecular epidemiology of hepatitis E virus in Hungary (2018\u20132025): Emergence of rare subtypes and first detection of HEV-4 in central Europe. Viruses, 17.","DOI":"10.3390\/v17101389"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Minosse, C., Biliotti, E., Lapa, D., Rianda, A., Marchili, M., Luzzitelli, I., Capobianchi, M.R., McPhee, F., Garbuglia, A.R., and D\u2019Offizi, G. (2020). Clinical characteristics of acute hepatitis E and their correlation with HEV genotype 3 subtypes in Italy. Pathogens, 9.","DOI":"10.3390\/pathogens9100832"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s15010-024-02348-0","article-title":"HEV-3 subtypes and strains detected in cases of HEV infection in central Italy from 2015 to 2023","volume":"53","author":"Garbuglia","year":"2025","journal-title":"Infection"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"30299","DOI":"10.2807\/1560-7917.ES.2016.21.30.30299","article-title":"High prevalence of anti-hepatitis E virus antibodies among blood donors in central Italy; February to March 2014","volume":"21","author":"Lucarelli","year":"2016","journal-title":"Eurosurveillance"},{"key":"ref_13","first-page":"413","article-title":"A nationwide retrospective study on prevalence of hepatitis E virus infection in italian blood donors","volume":"16","author":"Spada","year":"2018","journal-title":"Blood Transfus."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2100516","DOI":"10.2807\/1560-7917.ES.2022.27.22.2100516","article-title":"Prevalence and risk factors for hepatitis E virus infection in blood donors: A nationwide survey in Italy, 2017 to 2019","volume":"27","author":"Spada","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1111\/tbed.12533","article-title":"Hepatitis E virus: A cross-sectional serological and virological study in pigs and humans at zoonotic risk within a high-density pig farming area","volume":"64","author":"Caruso","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_16","first-page":"1","article-title":"Proposal for a new subtype of the zoonotic genotype 3 hepatitis E virus: HEV-3l","volume":"248","author":"Lemey","year":"2018","journal-title":"Virus Res."},{"key":"ref_17","first-page":"2806835","article-title":"Molecular characterization and phylogenetic analysis of hepatitis E virus (HEV) strains from pigs farmed in eight european countries between 2020 and 2022","volume":"2023","author":"Ianiro","year":"2023","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1007\/s12560-024-09606-2","article-title":"Hepatitis E virus RNA detection in liver and muscle tissues sampled from home slaughtered domestic pigs in central Italy","volume":"16","author":"Ferri","year":"2024","journal-title":"Food Environ. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"110068","DOI":"10.1016\/j.ijfoodmicro.2022.110068","article-title":"Epidemiological and genetic evaluation of HEV in swine slaughtered in Sicily region (Italy)","volume":"388","author":"Lorusso","year":"2023","journal-title":"Int. J. Food Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s00705-014-2246-5","article-title":"Serological and virological survey of hepatitis E virus in wild boar populations in northwestern Italy: Detection of HEV subtypes 3e and 3f","volume":"160","author":"Caruso","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/s12560-015-9201-9","article-title":"Molecular investigation on the presence of hepatitis E virus (HEV) in wild game in north-western Italy","volume":"7","author":"Serracca","year":"2015","journal-title":"Food Environ. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.1007\/s00705-016-2964-y","article-title":"Detection and genetic characterization of hepatitis E virus (HEV) genotype 3 subtype c in wild boars in Italy","volume":"161","author":"Melegari","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1007\/s12560-021-09467-z","article-title":"Hepatitis E virus (HEV) spread and genetic diversity in game animals in northern Italy","volume":"13","author":"Arnaboldi","year":"2021","journal-title":"Food Environ. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.meegid.2019.03.005","article-title":"Identification of a zoonotic genotype 3 hepatitis E subtype in wildlife in north-eastern Italy","volume":"71","author":"Zecchin","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e25","DOI":"10.1111\/tbed.12661","article-title":"Molecular detection and phylogenetic analysis of hepatitis E virus strains circulating in wild boars in south-central Italy","volume":"65","author":"Aprea","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e05110","DOI":"10.1016\/j.heliyon.2020.e05110","article-title":"Phylogenetic analysis and epidemiological history of hepatitis E virus 3f and 3c in swine and wild boar, Italy","volume":"6","author":"Bruni","year":"2020","journal-title":"Heliyon"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Aprea, G., Scattolini, S., D\u2019Angelantonio, D., Chiaverini, A., Di Lollo, V., Olivieri, S., Marcacci, M., Mangone, I., Salucci, S., and Antoci, S. (2020). Whole genome sequencing characterization of HEV3-e and HEV3-f subtypes among the wild boar population in the Abruzzo region, Italy: First Report. Microorganisms, 8.","DOI":"10.3390\/microorganisms8091393"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Scattolini, S., Equestre, M., Curini, V., Bruni, R., Di Lollo, V., Secondini, B., Marcantonio, C., Madonna, E., Ciccaglione, A.R., and Camma, C. (2026, June 22). Genomic Investigation of Hepatitis E Virus Genotype 3 Circulation in the Animal Reservoir in Central Italy by a One Health Approach. 2026. 14p. Pre-Print Article (Not Peer Reviewed). Available online: https:\/\/papers.ssrn.com\/sol3\/papers.cfm?abstract_id=6255015.","DOI":"10.2139\/ssrn.6255015"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ferri, G., Lauteri, C., Festino, A.R., Piccinini, A., Olivastri, A., and Vergara, A. (2022). Hepatitis E virus detection in hunted wild boar liver and muscle tissues in central Italy. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081628"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/s12560-023-09554-3","article-title":"Hepatitis E virus RNA detection from hunted wild boars in central Italy: An epidemiological investigation","volume":"15","author":"Ferri","year":"2023","journal-title":"Food Environ. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1111\/tbed.12948","article-title":"Molecular survey of HEV infection in wild boar population in Italy","volume":"65","author":"Ostanello","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.fm.2019.02.005","article-title":"Quantification and genetic diversity of hepatitis E virus in wild boar (Sus scrofa) hunted for domestic consumption in central Italy","volume":"82","author":"Iaconelli","year":"2019","journal-title":"Food Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1007\/s11262-018-1597-5","article-title":"Phylogenetic analysis of two genotype 3 hepatitis E viruses from wild boar, Italy","volume":"54","author":"Vaccari","year":"2018","journal-title":"Virus Genes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1111\/tbed.13860","article-title":"Hepatitis E virus (HEV) genotype 3 diversity: Identification of a novel HEV subtype in wild boar in central Italy","volume":"68","author":"Pierini","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"La Bella, G., Basanisi, M.G., Nobili, G., Coppola, R., Damato, A.M., Donatiello, A., Occhiochiuso, G., Romano, A.C., Toce, M., and Palazzo, L. (2023). Evidence of circulation and phylogenetic analysis of hepatitis E virus (HEV) in wild boar in south-east Italy. Viruses, 15.","DOI":"10.3390\/v15102021"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"109671","DOI":"10.1016\/j.ijfoodmicro.2022.109671","article-title":"Occurrence of hepatitis E virus (HEV) in calabrian wild boars","volume":"371","author":"Lorusso","year":"2022","journal-title":"Int. J. Food Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1111\/tbed.13157","article-title":"Hepatitis E virus in sheep in Italy","volume":"66","author":"Sarchese","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.virusres.2016.09.008","article-title":"Detection of hepatitis E virus (HEV) in goats","volume":"225","author":"Melegari","year":"2016","journal-title":"Virus Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3126419","DOI":"10.1155\/2023\/3126419","article-title":"High prevalence and genetic heterogeneity of genotype 3 hepatitis E virus in wild boar in Umbria, central Italy","volume":"2023","author":"Beikpour","year":"2023","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ijfoodmicro.2017.06.029","article-title":"Porcine blood used as ingredient in meat productions may serve as a vehicle for hepatitis E virus transmission","volume":"257","author":"Boxman","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jviromet.2005.07.004","article-title":"A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus","volume":"131","author":"Jothikumar","year":"2006","journal-title":"J. Virol. Methods"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/jmv.10276","article-title":"Molecular investigation of hepatitis E virus infection in patients with acute hepatitis in Kathmandu, Nepal","volume":"69","author":"Shrestha","year":"2003","journal-title":"J. Med. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"msae263","DOI":"10.1093\/molbev\/msae263","article-title":"MEGA12: Molecular Evolutionary Genetic Analysis version 12 for adaptive and green computing","volume":"41","author":"Kumar","year":"2024","journal-title":"Mol. Biol. Evol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_45","unstructured":"(2025, November 13). Canva. Strumento per Creare Grafici (Tool for Creating Graphs). Available online: https:\/\/canva.com\/it_it\/diagramma\/."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/709\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:02:16Z","timestamp":1782799336000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,27]]},"references-count":45,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["v18070709"],"URL":"https:\/\/doi.org\/10.3390\/v18070709","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,27]]}}}