{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:44:40Z","timestamp":1785433480142,"version":"3.56.0"},"reference-count":113,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, Portugal","award":["2022.13408.BD"],"award-info":[{"award-number":["2022.13408.BD"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, Portugal","award":["UID\/00102\/2025"],"award-info":[{"award-number":["UID\/00102\/2025"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, Portugal","award":["UID\/PRR\/00102\/2025"],"award-info":[{"award-number":["UID\/PRR\/00102\/2025"]}]},{"award":["2022.13408.BD"],"award-info":[{"award-number":["2022.13408.BD"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UID\/00102\/2025"],"award-info":[{"award-number":["UID\/00102\/2025"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UID\/PRR\/00102\/2025"],"award-info":[{"award-number":["UID\/PRR\/00102\/2025"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>JC polyomavirus (JCPyV) is a worldwide-distributed human virus. Primary infection with JCPyV is usually asymptomatic and followed by a lifelong persistent infection. In situations of profound immunosuppression or prolonged treatment with specific immunomodulatory drugs, such as natalizumab, viral reactivation can occur and lead to the development of JCPyV-associated diseases. Progressive multifocal leukoencephalopathy (PML), a severe demyelinating disease of the central nervous system, is the most common clinical manifestation of JCPyV reactivation. Less frequently, viral reactivation may be associated with granule cell neuronopathy, encephalopathy, and meningitis. However, the pathogenesis of these diseases remains a subject of debate. To date, no treatment is available for JCPyV infection. Nevertheless, some therapeutic options have been explored. Despite its ubiquity, the main mode of JCPyV transmission remains unclear. Epidemiological data suggests that primary infection may be acquired in childhood and throughout life, with the involvement of different routes of transmission. In the absence of an effective treatment, the prevention of infection is crucial in risk groups, such as immunosuppressed or natalizumab-treated patients. Therefore, until the achievement of an effective antiviral molecule or a prophylactic vaccine, prevention measures will rely on avoiding transmission, for which it is crucial to understand how transmission occurs. The present review emphasizes the current data on JCPyV transmission routes and associated diseases, including pathogenesis, diagnosis and potential treatment options, highlighting the importance of further studies.<\/jats:p>","DOI":"10.3390\/v18070716","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T05:58:04Z","timestamp":1782799084000},"page":"716","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Current Perspectives on JC Polyomavirus Transmission and Associated Diseases: Implications for Prevention in Risk Populations"],"prefix":"10.3390","volume":"18","author":[{"given":"Joana M","family":"Oliveira","sequence":"first","affiliation":[{"name":"CERES\u2014Chemical Engineering and Renewable Resources for Sustainability, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Centre for Functional Ecology (CFE), Associate Laboratory TERRA, Department of Life Sciences, University of Coimbra, 3000-504 Coimbra, Portugal"},{"name":"Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Luxo","sequence":"additional","affiliation":[{"name":"CERES\u2014Chemical Engineering and Renewable Resources for Sustainability, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M","family":"Matos","sequence":"additional","affiliation":[{"name":"CERES\u2014Chemical Engineering and Renewable Resources for Sustainability, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Laboratory of Clinical Analysis of University of Coimbra, 3004-504 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Moens, U., Prezioso, C., and Pietropaolo, V. (2020). Genetic Diversity of the Noncoding Control Region of the Novel Human Polyomaviruses. Viruses, 12.","DOI":"10.3390\/v12121406"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1128\/microbiolspec.DMIH2-0010-2015","article-title":"Polyomaviruses","volume":"4","author":"Cook","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.idc.2020.02.007","article-title":"JC Polyomavirus Infection Potentiated by Biologics","volume":"34","author":"Multani","year":"2020","journal-title":"Infect. Dis. Clin. North Am."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1007\/s13365-023-01164-w","article-title":"Revisiting JC Virus and Progressive Multifocal Leukoencephalopathy","volume":"29","author":"Rocchi","year":"2023","journal-title":"J. Neurovirol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S1474-4422(10)70040-5","article-title":"Progressive Multifocal Leukoencephalopathy and Other Disorders Caused by JC Virus: Clinical Features and Pathogenesis","volume":"9","author":"Tan","year":"2010","journal-title":"Lancet Neurol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41582-020-00427-y","article-title":"Progressive Multifocal Leukoencephalopathy and the Spectrum of JC Virus-Related Disease","volume":"17","author":"Cortese","year":"2021","journal-title":"Nat. Rev. Neurol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1002\/jmv.21710","article-title":"Characterization of JC Human Polyomavirus Infection in a Portuguese Population","volume":"82","author":"Matos","year":"2010","journal-title":"J. Med. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"116426","DOI":"10.1016\/j.jns.2019.116426","article-title":"JC Virus Antibodies in Portuguese Multiple Sclerosis Patients: JUSTIFY Study Results","volume":"406","author":"Nunes","year":"2019","journal-title":"J. Neurol. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jns.2016.03.050","article-title":"Anti-JC Virus Seroprevalence in a Spanish Multiple Sclerosis Cohort JC Virus Seroprevalence in Spain","volume":"365","author":"Aladro","year":"2016","journal-title":"J. Neurol. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1128\/CVI.05175-11","article-title":"Age-Specific Seroprevalence of Merkel Cell Polyomavirus, BK Virus, and JC Virus","volume":"18","author":"Viscidi","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/s41598-023-27850-7","article-title":"Seroprevalence of Polyomaviruses BK and JC in Finnish Women and Their Spouses Followed-up for Three Years","volume":"13","author":"Laine","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Vilibic-Cavlek, T., Bogdanic, M., Peric, T., Radmanic, L., Antolasic, L., Milasincic, L., and Zidovec-Lepej, S. (2024). Prevalence of JC Polyomavirus in Patients with Neuroinvasive Disease of Unknown Etiology in Croatia. Medicina, 60.","DOI":"10.3390\/medicina60010069"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1099\/vir.0.020115-0","article-title":"Prevalence and Stability of Antibodies to the BK and JC Polyomaviruses: A Long-Term Longitudinal Study of Australians","volume":"91","author":"Antonsson","year":"2010","journal-title":"J. Gen. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kamminga, S., Van Der Meijden, E., Feltkamp, M.C.W., and Zaaijer, H.L. (2018). Seroprevalence of Fourteen Human Polyomaviruses Determined in Blood Donors. PLoS ONE, 13.","DOI":"10.1101\/357350"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s13365-016-0477-9","article-title":"JC Virus Infection Is Acquired Very Early in Life: Evidence from a Longitudinal Serological Study","volume":"23","author":"Elia","year":"2017","journal-title":"J. Neurovirol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kean, J.M., Rao, S., Wang, M., and Garcea, R.L. (2009). Seroepidemiology of Human Polyomaviruses. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000363"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kimla, L.J., and Segal, D. (2026). JC Polyomavirus (JCV, HPyV2) Seropositivity Prevalence in Healthy Subjects: Systematic Review and Meta-Analysis. PLoS ONE, 21.","DOI":"10.1371\/journal.pone.0341146"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e38996","DOI":"10.1016\/j.heliyon.2024.e38996","article-title":"Identification of JC Polyomavirus in Upper Respiratory Samples from Portuguese Children","volume":"10","author":"Oliveira","year":"2024","journal-title":"Heliyon"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"117368","DOI":"10.1016\/j.ecoenv.2024.117368","article-title":"Municipal Secondary-Treated Effluent Data Seem to Be a Suitable Source of Information for Human Viral Trends","volume":"288","author":"Rodrigues","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.jcv.2009.05.002","article-title":"Detection of BK, JC, WU, or KI Polyomaviruses in Faecal, Urine, Blood, Cerebrospinal Fluid and Respiratory Samples","volume":"45","author":"Bialasiewicz","year":"2009","journal-title":"J. Clin. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s12560-021-09496-8","article-title":"Assessment of Microbiological Quality of Fresh Vegetables and Oysters Produced in Buenos Aires Province, Argentina","volume":"13","author":"Cammarata","year":"2021","journal-title":"Food Environ. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.watres.2016.09.010","article-title":"Human Polyomavirus: Advantages and Limitations as a Human-Specific Viral Marker in Aquatic Environments","volume":"105","author":"Rachmadi","year":"2016","journal-title":"Water Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"107992","DOI":"10.1016\/j.ijid.2025.107992","article-title":"First Detection of JC Polyomavirus in Vaginal Secretions from Non-Pregnant Women: Exploring Sexual Transmission as a Potential Route of Infection","volume":"159","author":"Oliveira","year":"2025","journal-title":"Int. J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Comar, M., Zanotta, N., Croci, E., Murru, I., Marci, R., Pancaldi, C., Dolcet, O., Luppi, S., Martinelli, M., and Giolo, E. (2012). Association between the JC Polyomavirus Infection and Male Infertility. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0042880"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1002\/jcp.25686","article-title":"Tracing Males From Different Continents by Genotyping JC Polyomavirus in DNA From Semen Samples","volume":"232","author":"Rotondo","year":"2017","journal-title":"J. Cell. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/aje\/kwv155","article-title":"Seroepidemiology of Human Polyomaviruses in a US Population","volume":"183","author":"Gossai","year":"2016","journal-title":"Am. J. Epidemiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1158\/1055-9965.EPI-15-1111","article-title":"Prospective Study of Human Polyomaviruses and Risk of Cutaneous Squamous Cell Carcinoma in the United States","volume":"25","author":"Gossai","year":"2016","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1080\/13550280260422758","article-title":"High Incidence of JC Viruria in JC-Seropositive Older Individuals","volume":"8","author":"Chang","year":"2002","journal-title":"J. Neurovirol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1038\/bjc.2016.285","article-title":"Serum Biomarkers of Polyomavirus Infection and Risk of Lung Cancer in Never Smokers","volume":"115","author":"Malhotra","year":"2016","journal-title":"Br. J. Cancer"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1093\/aje\/kwv281","article-title":"The Natural History of Human Polyomaviruses and Herpesviruses in Early Life\u2014The Rhea Birth Cohort in Greece","volume":"183","author":"Karachaliou","year":"2016","journal-title":"Am. J. Epidemiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1086\/597126","article-title":"Prevalence of Polyomavirus BK and JC Infection and Replication in 400 Healthy Blood Donors","volume":"199","author":"Egli","year":"2009","journal-title":"J. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5513","DOI":"10.1002\/jcp.26457","article-title":"Serum IgG Antibodies from Healthy Subjects up to 100 Years Old React to JC Polyomavirus","volume":"233","author":"Bononi","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1002\/jmv.23841","article-title":"Seroprevalence Rates of BKV, JCV, and MCPyV Polyomaviruses in the General Czech Republic Population","volume":"86","author":"Fraiberk","year":"2014","journal-title":"J. Med. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1099\/vir.0.18842-0","article-title":"Seroepidemiology of the Human Polyomaviruses","volume":"84","author":"Stolt","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1002\/ijc.21277","article-title":"Antibodies to JC and BK Viruses among Persons with Non-Hodgkin Lymphoma","volume":"117","author":"Engels","year":"2005","journal-title":"Int. J. Cancer"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.meegid.2017.06.011","article-title":"Biology, Evolution, and Medical Importance of Polyomaviruses: An Update","volume":"54","author":"Moens","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.virol.2017.03.007","article-title":"Isolation and Characterization of a Novel Putative Human Polyomavirus","volume":"506","author":"Gheit","year":"2017","journal-title":"Virology"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Saribas, S., and Safak, M. (2020). A Comprehensive Proteomics Analysis of the JC Virus (JCV) Large and Small Tumor Antigen Interacting Proteins: Large t Primarily Targets the Host Protein Complexes with V-ATPase and Ubiquitin Ligase Activities While Small t Mostly Associates with Those Having Phosphatase and Chromatin-Remodeling Functions. Viruses, 12.","DOI":"10.3390\/v12101192"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1111\/j.1348-0421.2012.00484.x","article-title":"Role of JC Virus Agnoprotein in Virion Formation","volume":"56","author":"Suzuki","year":"2012","journal-title":"Microbiol. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"8295","DOI":"10.1002\/jcp.27715","article-title":"Expression of Novel Proteins by Polyomaviruses and Recent Advances in the Structural and Functional Features of Agnoprotein of JC Virus, BK Virus, and Simian Virus 40","volume":"234","author":"Saribas","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.virol.2020.11.004","article-title":"Human Neurotropic Polyomavirus, JC Virus, Agnoprotein Targets Mitochondrion and Modulates Its Functions","volume":"553","author":"Saxena","year":"2021","journal-title":"Virology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1128\/JVI.01680-07","article-title":"JC Virus Agnoprotein Inhibits In Vitro Differentiation of Oligodendrocytes and Promotes Apoptosis","volume":"82","author":"Merabova","year":"2008","journal-title":"J. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.virol.2014.07.054","article-title":"The Agnoprotein of Polyomavirus JC Is Released by Infected Cells: Evidence for Its Cellular Uptake by Uninfected Neighboring Cells","volume":"468\u2013470","author":"Otlu","year":"2014","journal-title":"Virology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/s40588-017-0069-3","article-title":"JC Polyomavirus Attachment and Entry: Potential Sites for PML Therapeutics","volume":"4","author":"Mayberry","year":"2017","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"ref_45","first-page":"177","article-title":"Cellular Entry of Polyomaviruses","volume":"343","author":"Tsai","year":"2010","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"DuShane, J.K., Mayberry, C.L., Wilczek, M.P., Nichols, S.L., and Maginnis, M.S. (2019). JCPyV-Induced MAPK Signaling Activates Transcription Factors during Infection. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194779"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1080\/13550280152537201","article-title":"Predicted Amino Acid Sequences for 100 JCV Strains","volume":"7","author":"Cubitt","year":"2001","journal-title":"J. Neurovirol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1086\/317927","article-title":"JC Virus Genotypes in France: Molecular Epidemiology and Potential Significance for Progressive Multifocal Leukoencephalopathy","volume":"183","author":"Dubois","year":"2001","journal-title":"J. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s13365-025-01262-x","article-title":"Molecular Epidemiology of JC Polyomavirus Genotypes in PLWH from Turkey","volume":"31","author":"Mete","year":"2025","journal-title":"J. Neurovirol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1097\/00002030-200304110-00004","article-title":"Polyomavirus JCV Excretion and Genotype Analysis in HIV-Infected Patients Receiving Highly Active Antiretroviral Therapy","volume":"17","author":"Lednicky","year":"2003","journal-title":"AIDS"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1002\/jmv.23796","article-title":"Distribution of JC Virus Genotypes among Serbian Patients Infected with HIV and in Healthy Donors","volume":"86","author":"Karalic","year":"2014","journal-title":"J. Med. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1002\/jmv.23585","article-title":"Molecular Epidemiology of JCV Genotypes in Patients and Healthy Subjects from Northern Italy","volume":"85","author":"Zanotta","year":"2013","journal-title":"J. Med. Virol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Yang, J.F., and You, J. (2020). Regulation of Polyomavirus Transcription by Viral and Cellular Factors. Viruses, 12.","DOI":"10.3390\/v12101072"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1093\/infdis\/jiq097","article-title":"Pathogenesis of Progressive Multifocal Leukoencephalopathy\u2014Revisited","volume":"203","author":"White","year":"2011","journal-title":"J. Infect. Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"839719","DOI":"10.1155\/2013\/839719","article-title":"New Insights on Human Polyomavirus JC and Pathogenesis of Progressive Multifocal Leukoencephalopathy","volume":"2013","author":"Bellizzi","year":"2013","journal-title":"Clin. Dev. Immunol."},{"key":"ref_56","first-page":"179","article-title":"John Cunningham Virus: An Overview on Biology and Disease of the Etiological Agent of the Progressive Multifocal Leukoencephalopathy","volume":"41","author":"Pietropaolo","year":"2018","journal-title":"New Microbiol."},{"key":"ref_57","first-page":"24","article-title":"Progressive Multifocal Leukoencephalopathy-Epidemiology, Immune Response, Clinical Differences, Treatment","volume":"68","author":"Snopek","year":"2019","journal-title":"Epidemiol. Mikrobiol. Imunol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"10290","DOI":"10.1128\/JVI.75.21.10290-10299.2001","article-title":"Potential Transmission of Human Polyomaviruses through the Gastrointestinal Tract after Exposure to Virions or Viral DNA","volume":"75","author":"Calafell","year":"2001","journal-title":"J. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"9918","DOI":"10.1128\/JVI.72.12.9918-9923.1998","article-title":"Detection of JC Virus DNA in Human Tonsil Tissue: Evidence for Site of Initial Viral Infection","volume":"72","author":"Monaco","year":"1998","journal-title":"J. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"7004","DOI":"10.1128\/jvi.70.10.7004-7012.1996","article-title":"JC Virus Infection of Hematopoietic Progenitor Cells, Primary B Lymphocytes, and Tonsillar Stromal Cells: Implications for Viral Latency","volume":"70","author":"Monaco","year":"1996","journal-title":"J. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e9","DOI":"10.1086\/504947","article-title":"JC Virus Detection in Bodily Fluids: Clues to Transmission","volume":"43","author":"Berger","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jcv.2017.02.003","article-title":"Polyomavirus BK and JC in Individuals with Chronic Kidney Failure, Kidney Transplantation, and Healthy Controls","volume":"89","author":"Castro","year":"2017","journal-title":"J. Clin. Virol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1111\/odi.12707","article-title":"Detection of Human Polyomaviruses JC and BK in Liver Pretransplant Patients","volume":"23","author":"Figueiredo","year":"2017","journal-title":"Oral Dis."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1099\/vir.0.036798-0","article-title":"Individuals Infected with JC Polyomavirus Do Not Present Detectable JC Virus DNA in Oropharyngeal Fluids","volume":"93","author":"Matos","year":"2012","journal-title":"J. Gen. Virol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s10096-018-3404-6","article-title":"Human Polyomavirus KI, WU, BK, and JC in Healthy Volunteers","volume":"38","author":"Pena","year":"2019","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/j.1469-0691.2004.00882.x","article-title":"Prevalence and Patterns of Polyomavirus Urinary Excretion in Immunocompetent Adults and Children","volume":"10","author":"Polo","year":"2004","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/S1386-6532(03)00155-0","article-title":"Detection of BK Virus and JC Virus DNA in Urine Samples from Immunocompromised (HIV-Infected) and Immunocompetent (HIV-Non-Infected) Patients Using Polymerase Chain Reaction and Microplate Hybridisation","volume":"29","author":"Klapper","year":"2004","journal-title":"J. Clin. Virol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.1128\/JCM.02472-08","article-title":"Polyomavirus Shedding in the Stool of Healthy Adults","volume":"47","author":"Vanchiere","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"3379","DOI":"10.1128\/AEM.02302-08","article-title":"Quantification of Human Polyomaviruses JC Virus and BK Virus by TaqMan Quantitative PCR and Comparison to Other Water Quality Indicators in Water and Fecal Samples","volume":"75","author":"McQuaig","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1080\/13550280390195306","article-title":"Role of the Environment in the Transmission of JC Virus","volume":"9","author":"Girones","year":"2003","journal-title":"J. Neurovirol."},{"key":"ref_71","first-page":"1920891","article-title":"Quantification of Human and Animal Viruses to Differentiate the Origin of the Fecal Contamination Present in Environmental Samples","volume":"2013","author":"Hundesa","year":"2013","journal-title":"BioMed Res. Int."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Gomes, J., Domingues, E., Frasson, D., Martins, R.C., and Matos, A.M. (2024). Virus Removal from Real Wastewater as an Environmental Management Approach. Molecules, 29.","DOI":"10.3390\/molecules29235601"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"104244","DOI":"10.1016\/j.msard.2022.104244","article-title":"JCV Seroconversion Rate during the SARS COVID-19 Pandemic","volume":"68","author":"Vigiser","year":"2022","journal-title":"Mult. Scler. Relat. Disord."},{"key":"ref_74","first-page":"100112","article-title":"Trends in Respiratory Virus Circulation Following COVID-19-Targeted Nonpharmaceutical Interventions in Germany, January\u2013September 2020: Analysis of National Surveillance Data","volume":"6","author":"Oh","year":"2021","journal-title":"Lancet Reg. Health-Eur."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1002\/(SICI)1096-9071(199812)56:4<372::AID-JMV14>3.0.CO;2-4","article-title":"Transplacental Transmission of Human Polyomavirus BK","volume":"56","author":"Pietropaolo","year":"1998","journal-title":"J. Med. Virol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1099\/vir.0.028571-0","article-title":"Serological Evidence of Vertical Transmission of JC and BK Polyomaviruses in Humans","volume":"92","author":"Boldorini","year":"2011","journal-title":"J. Gen. Virol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1080\/00313020701716458","article-title":"Latent Human Polyomavirus Infection in Pregnancy: Investigation of Possible Transplacental Transmission","volume":"40","author":"Boldorini","year":"2008","journal-title":"Pathology"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.jinf.2020.02.006","article-title":"Mother-to-Child Transmission of Oncogenic Polyomaviruses BKPyV, JCPyV and SV40","volume":"80","author":"Mazzoni","year":"2020","journal-title":"J. Infect."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Atkinson, A.L., and Atwood, W.J. (2020). Fifty Years of JC Polyomavirus: A Brief Overview and Remaining Questions. Viruses, 12.","DOI":"10.3390\/v12090969"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1002\/ana.24371","article-title":"Persistence and Pathogenesis of the Neurotropic Polyomavirus JC","volume":"77","author":"Wollebo","year":"2015","journal-title":"Ann. Neurol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s00401-015-1471-7","article-title":"Progressive Multifocal Leukoencephalopathy and Immune Reconstitution Inflammatory Syndrome (IRIS)","volume":"130","author":"Bauer","year":"2015","journal-title":"Acta Neuropathol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s00401-005-0983-y","article-title":"Fulminant Inflammatory Leukoencephalopathy Associated with HAART-Induced Immune Restoration in AIDS-Related Progressive Multifocal Leukoencephalopathy","volume":"109","author":"Vendrely","year":"2005","journal-title":"Acta Neuropathol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"969","DOI":"10.2165\/11537510-000000000-00000","article-title":"Progressive Multifocal Leukoencephalopathy and Newer Biological Agents","volume":"33","author":"Berger","year":"2010","journal-title":"Drug Saf."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1136\/jim-2016-000339","article-title":"Update on Disease-Modifying Therapies for Multiple Sclerosis","volume":"65","author":"Vargas","year":"2017","journal-title":"J. Investig. Med."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"259","DOI":"10.2147\/tcrm.2007.3.2.259","article-title":"Natalizumab: A New Treatment for Relapsing Remitting Multiple Sclerosis","volume":"3","author":"Hutchinson","year":"2007","journal-title":"Ther. Clin. Risk Manag."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1002\/ana.26198","article-title":"Advances in Treatment of Progressive Multifocal Leukoencephalopathy","volume":"90","author":"Koralnik","year":"2021","journal-title":"Ann. Neurol."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"van der Meijden, E., Janssens, R.W.A., Lauber, C., Bavinck, J.N.B., Gorbalenya, A.E., and Feltkamp, M.C.W. (2010). Discovery of a New Human Polyomavirus Associated with Trichodysplasia Spinulosa in an Immunocompromized Patient. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1001024"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1017\/cjn.2018.28","article-title":"JC Virus Granule Cell Neuronopathy as AIDS-Presenting Illness","volume":"45","author":"Dang","year":"2018","journal-title":"Can. J. Neurol. Sci."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1002\/ana.25608","article-title":"Fatal Encephalopathy with Wild-Type JC Virus and Ruxolitinib Therapy","volume":"86","author":"Reoma","year":"2019","journal-title":"Ann. Neurol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1002\/ana.24192","article-title":"A Fatal Case of JC Virus Meningitis Presenting with Hydrocephalus in a Human Immunodeficiency Virus-Seronegative Patient","volume":"76","author":"Agnihotri","year":"2014","journal-title":"Ann. Neurol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"105797","DOI":"10.1016\/j.micpath.2022.105797","article-title":"Association between Human Polyomavirus Infection and Brain Cancer: A Systematic Review and Meta-Analysis","volume":"173","author":"Far","year":"2022","journal-title":"Microb. Pathog."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1080\/23744235.2019.1692145","article-title":"Association between JC Virus and Colorectal Cancer: Systematic Review and Meta-Analysis","volume":"52","author":"Shavaleh","year":"2020","journal-title":"Infect. Dis."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Ki\u015b, J., Sikora, D., Jarosz, M.J., and Polz-Dacewicz, M. (2025). JC Polyomavirus in Prostate Cancer\u2014Friend or Foe?. Cancers, 17.","DOI":"10.20944\/preprints202504.0231.v1"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1002\/pros.10157","article-title":"Detection of Human Polyomaviruses and Papillomaviruses in Prostatictissue Reveals the Prostate as a Habitat for Multipleviral Infections","volume":"53","author":"Zambrano","year":"2002","journal-title":"Prostate"},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Tomb, R.M., McManus, S.K., Kipgen, D., Yaqub, S., Taylor, S., and Gunson, R.N. (2025). JC Polyomavirus-Associated Nephropathy Case Report: Clinical and Laboratory Learning. Br. J. Biomed. Sci., 82.","DOI":"10.3389\/bjbs.2025.14170"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"e12654","DOI":"10.1111\/tid.12654","article-title":"JC Polyomavirus Nephropathy, a Rare Cause of Transplant Dysfunction: Case Report and Review of Literature","volume":"19","author":"Yang","year":"2017","journal-title":"Transpl. Infect. Dis."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1399-3062.2010.00567.x","article-title":"JC Virus-Associated Nephropathy in a Renal Transplant Recipient and Comparative Analysis of Previous Cases","volume":"13","author":"Kantarci","year":"2011","journal-title":"Transpl. Infect. Dis."},{"key":"ref_98","first-page":"35","article-title":"Mirtazapine in an HIV-1 Infected Patient with Progressive Multifocal Leukoencephalopathy","volume":"17","author":"Lanzafame","year":"2009","journal-title":"Infez. Med."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1001\/archneurol.2008.557","article-title":"Mirtazapine Use in Human Immunodeficiency Virus-Infected Patients With Progressive Multifocal Leukoencephalopathy","volume":"66","author":"Cettomai","year":"2009","journal-title":"Arch. Neurol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1007\/s40261-016-0433-8","article-title":"Treatment of Progressive Multifocal Leukoencephalopathy With Mirtazapine","volume":"36","author":"Jamilloux","year":"2016","journal-title":"Clin. Drug Investig."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s13365-013-0173-y","article-title":"A Study of Mefloquine Treatment for Progressive Multifocal Leukoencephalopathy: Results and Exploration of Predictors of PML Outcomes","volume":"19","author":"Clifford","year":"2013","journal-title":"J. Neurovirol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"e213","DOI":"10.1212\/NXI.0000000000000213","article-title":"Interleukin-7 Treatment of PML in a Patient with Idiopathic Lymphocytopenia","volume":"3","author":"Miskin","year":"2016","journal-title":"Neurol. Neuroimmunol. Neuroinflamm."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1093\/cid\/ciu682","article-title":"Treating Progressive Multifocal Leukoencephalopathy with Interleukin 7 and Vaccination with JC Virus Capsid Protein VP1","volume":"59","author":"Sospedra","year":"2014","journal-title":"Clin. Infect. Dis."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/nri2970","article-title":"Harnessing the Biology of IL-7 for Therapeutic Application","volume":"11","author":"MacKall","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1007\/s13365-018-0657-x","article-title":"Successful Treatment of Progressive Multifocal Leukoencephalopathy with Recombinant Interleukin-7 and Maraviroc in a Patient with Idiopathic CD4 Lymphocytopenia","volume":"24","author":"Harel","year":"2018","journal-title":"J. Neurovirol."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Kanse, S., Khandelwal, M., Pandey, R.K., Khokhar, M., Desai, N., and Kumbhar, B.V. (2023). Designing a Multi-Epitope Subunit Vaccine against VP1 Major Coat Protein of JC Polyomavirus. Vaccines, 11.","DOI":"10.20944\/preprints202304.1067.v1"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"429","DOI":"10.2217\/imt-2018-0110","article-title":"Programmed Cell Death-1\/Programmed Cell Death Ligand-1 Checkpoint Inhibitors: Differences in Mechanism of Action","volume":"11","author":"Zhang","year":"2019","journal-title":"Immunotherapy"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1056\/NEJMoa1815039","article-title":"Pembrolizumab Treatment for Progressive Multifocal Leukoencephalopathy","volume":"380","author":"Cortese","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_109","first-page":"1","article-title":"Novel Treatments for Progressive Multifocal Leukoencephalopathy","volume":"81","author":"Bennett","year":"2020","journal-title":"Br. J. Hosp. Med."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1038\/bmt.2010.221","article-title":"Polyomavirus JC-Targeted T-Cell Therapy for Progressive Multiple Leukoencephalopathy in a Hematopoietic Cell Transplantation Recipient","volume":"46","author":"Balduzzi","year":"2011","journal-title":"Bone Marrow Transpl."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1056\/NEJMoa1801540","article-title":"Allogeneic BK Virus\u2013Specific T Cells for Progressive Multifocal Leukoencephalopathy","volume":"379","author":"Muftuoglu","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1126\/science.1152586","article-title":"Clonal Integration of a Polyomavirus in Human Merkel Cell Carcinoma","volume":"319","author":"Feng","year":"2008","journal-title":"Science"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s13073-020-00727-4","article-title":"Clinical and Molecular Characterization of Virus-Positive and Virus-Negative Merkel Cell Carcinoma","volume":"12","author":"Starrett","year":"2020","journal-title":"Genome Med."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/716\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:05:31Z","timestamp":1782799531000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":113,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["v18070716"],"URL":"https:\/\/doi.org\/10.3390\/v18070716","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,29]]}}}