{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:23:16Z","timestamp":1783009396081,"version":"3.54.5"},"reference-count":42,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Canine distemper virus (CDV), currently classified within the species Morbillivirus canis, is a major vaccine-preventable pathogen of domestic dogs and a wide range of susceptible wildlife species. Still, laboratory-confirmed epidemiological data from Portugal remain scarce. This retrospective study investigated CDV molecular detection in 637 diagnostic samples from dogs with clinical suspicion of canine distemper, received from 190 veterinary medical centres across Portugal between 2013 and 2025. Cerebrospinal fluid, EDTA-anticoagulated whole blood, rectal swabs, and conjunctival swabs were tested for CDV RNA using a reverse transcription real-time PCR (RT-qPCR) assay in a qualitative approach. Overall, 215 submissions were CDV RT-qPCR-positive, corresponding to a positivity of 33.8% (95% confidence interval: 30.2\u201337.5%). Positivity was not significantly associated with sex, age, or Nomenclature of Territorial Units for Statistics level 2 (NUTS 2) region, but differed significantly according to specimen type, with the highest detection observed in EDTA-anticoagulated whole blood and conjunctival swabs. Mixed-breed dogs were over-represented among submitted samples and positive cases, probably reflecting management, exposure, and vaccination-related factors rather than intrinsic breed susceptibility. The central finding was a pronounced post-2020 decline in CDV RT-qPCR positivity, with very low or absent annual detection between 2021 and 2025. This pattern indicates reduced molecular detection within a passive diagnostic population but should not be interpreted as evidence of national elimination. Continued vaccination and strengthened surveillance at the domestic dog\u2013wildlife interface remain essential.<\/jats:p>","DOI":"10.3390\/v18070734","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T15:52:24Z","timestamp":1783007544000},"page":"734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection of Canine Distemper Virus in Portugal: What Explains the Post-2020 Decline? A Retrospective RT-qPCR Study"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9339-9932","authenticated-orcid":false,"given":"Ricardo","family":"Lopes","sequence":"first","affiliation":[{"name":"CEDIVET Veterinary Laboratories, Lionesa Business Hub, R. Lionesa 446 C24, 4465-671 Le\u00e7a do Balio, Portugal"},{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Department of Veterinary and Animal Sciences, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, Portugal"},{"name":"UNIPRO\u2014Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, Portugal"},{"name":"Molecular Diagnostics Laboratory, Unilabs Portugal, Lionesa Business Hub, R. Lionesa, 4465-671 Le\u00e7a do Balio, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Costa","family":"Santos","sequence":"additional","affiliation":[{"name":"RISE-Health, Department of Community Medicine, Information and Health Decision Sciences, Faculty of Medicine, University of Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5711-1905","authenticated-orcid":false,"given":"Hugo","family":"Lima de Carvalho","sequence":"additional","affiliation":[{"name":"CEDIVET Veterinary Laboratories, Lionesa Business Hub, R. Lionesa 446 C24, 4465-671 Le\u00e7a do Balio, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0206-6903","authenticated-orcid":false,"given":"Filipe","family":"Sampaio","sequence":"additional","affiliation":[{"name":"CEDIVET Veterinary Laboratories, Lionesa Business Hub, R. Lionesa 446 C24, 4465-671 Le\u00e7a do Balio, Portugal"},{"name":"Cytology and Hematology Diagnostic Services, Laboratory of Histology and Embryology, Department of Microscopy, ICBAS-School of Medicine and Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5031-679X","authenticated-orcid":false,"given":"C\u00e1tia","family":"Fernandes","sequence":"additional","affiliation":[{"name":"AniCura Santa Marinha Veterinary Hospital, R. Dom Henrique de Cernache 183, 4400-625 Vila Nova de Gaia, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7940-3141","authenticated-orcid":false,"given":"Andreia","family":"Garc\u00eas","sequence":"additional","affiliation":[{"name":"Wildlife Rehabilitation Centre (CRAS), Veterinary Teaching Hospital, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0708-6874","authenticated-orcid":false,"given":"Carlos","family":"Sousa","sequence":"additional","affiliation":[{"name":"Molecular Pathology Laboratory, SYNLAB Portugal, Av. Columbano Bordalo Pinheiro, 75A, 1070-061 Lisboa, Portugal"},{"name":"PerMed Research Group, RISE-Health, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Rita","family":"Silva","sequence":"additional","affiliation":[{"name":"Molecular Pathology Laboratory, SYNLAB Portugal, Av. Columbano Bordalo Pinheiro, 75A, 1070-061 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8098-7401","authenticated-orcid":false,"given":"Hugo","family":"Silva","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics Laboratory, Unilabs Portugal, Lionesa Business Hub, R. Lionesa, 4465-671 Le\u00e7a do Balio, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6145-7560","authenticated-orcid":false,"given":"Lu\u00eds","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuno","family":"Alegria","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0589-3533","authenticated-orcid":false,"given":"Elsa Leclerc","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Science and Technology, University of \u00c9vora, Polo da Mitra, Apartado 94, 7002-554 \u00c9vora, Portugal"},{"name":"Mediterranean Institute for Agriculture, Environment and Development (MED), Global Change and Sustainability Institute (CHANGE), University of \u00c9vora, Polo da Mitra, Apartado 94, 7002-554 \u00c9vora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7196-4179","authenticated-orcid":false,"given":"Ana Cl\u00e1udia","family":"Coelho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Candela, M.G., and Ortega, N. (2025). Canine distemper virus: Special issue editorial. Viruses, 17.","DOI":"10.3390\/v17121630"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"725","DOI":"10.7589\/0090-3558-47.3.725","article-title":"Domestic dog origin of canine distemper virus in free-ranging wolves in Portugal as revealed by hemagglutinin gene characterization","volume":"47","author":"Silva","year":"2011","journal-title":"J. Wildl. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6632068","DOI":"10.1155\/tbed\/6632068","article-title":"Geographical distribution of carnivore hosts and genotypes of canine distemper virus (CDV) worldwide: A scoping review and spatial meta-analysis","volume":"2025","author":"Wipf","year":"2025","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"O\u011fuzo\u011flu, T.\u00c7., and Ko\u00e7, B.T. (2025). Global phylogenetic analysis of the CDV hemagglutinin gene reveals positive selection on key receptor-binding sites. Viruses, 17.","DOI":"10.3390\/v17091149"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Liu, X., Ozkan, I.E., Naeem, R., Umar, S., Kayar, A., Tali, H.E., Y\u0131lmaz, A., Richt, J.A., Turan, N., and Yilmaz, H. (2025). Molecular epidemiology and emerging genotypes of canine distemper virus in Istanbul, T\u00fcrkiye. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-04963-x"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Wilkes, R.P. (2022). Canine distemper virus in endangered species: Species jump, clinical variations, and vaccination. Pathogens, 12.","DOI":"10.3390\/pathogens12010057"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Alfano, F., Lucibelli, M.G., D\u2019Alessio, N., Auriemma, C., Rea, S., Sgroi, G., Lucente, M.S., Pellegrini, F., Diakoudi, G., and De Carlo, E. (2025). Detection of canine distemper virus in wildlife in Italy (2022\u20132024). Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1527550"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/jsap.13718","article-title":"2024 Guidelines for the vaccination of dogs and cats-compiled by the Vaccination Guidelines Group (VGG) of the World Small Animal Veterinary Association (WSAVA)","volume":"65","author":"Squires","year":"2024","journal-title":"J. Small Anim. Pract."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Huang, J., Cortey, M., Darwich, L., Griffin, J., Ob\u00f3n, E., Molina, R., and Mart\u00edn, M. (2024). Study of canine distemper virus presence in Catalonia\u2019s wild carnivores through H gene amplification and sequencing. Animals, 14.","DOI":"10.3390\/ani14030436"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Prpi\u0107, J., Lojki\u0107, I., Keros, T., Kre\u0161i\u0107, N., and Jemer\u0161i\u0107, L. (2023). Canine Distemper virus infection in the free-living wild canines, the red fox (Vulpes vulpes) and jackal (Canis aureus moreoticus), in Croatia. Pathogens, 12.","DOI":"10.3390\/pathogens12060833"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"753","DOI":"10.7589\/JWD-D-23-00157","article-title":"A canine distemper outbreak in urban red foxes (Vulpes vulpes) in Brussels, Belgium, 2020","volume":"60","author":"Tavernier","year":"2024","journal-title":"J. Wildl. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"221","DOI":"10.7589\/0090-3558-45.1.221","article-title":"Serologic survey for canine distemper virus and canine parvovirus in free-ranging wild carnivores from Portugal","volume":"45","author":"Santos","year":"2009","journal-title":"J. Wildl. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.vetmic.2010.04.024","article-title":"Importance of Canine Distemper Virus (CDV) infection in free-ranging Iberian lynxes (Lynx pardinus)","volume":"146","author":"Meli","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.onehlt.2015.09.002","article-title":"Cross-species transmission of canine distemper virus\u2014An update","volume":"1","author":"Beineke","year":"2015","journal-title":"One Health"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s12985-019-1136-6","article-title":"Tropism and molecular pathogenesis of canine distemper virus","volume":"16","author":"Canal","year":"2019","journal-title":"Virol. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"163860","DOI":"10.1155\/2012\/163860","article-title":"Immunopathogenic and neurological mechanisms of canine distemper virus","volume":"2012","author":"Carvalho","year":"2012","journal-title":"Adv. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Van, T.M., Tran, D.T.A., Nguyen, C.T.P., Huynh, G.T., Luu, M.T.N., Le, T.Q., and Tran, B.N. (2025). Epidemiological, clinical, and molecular insights into canine distemper virus in the Mekong delta region of Vietnam. Viruses, 17.","DOI":"10.3390\/v17060781"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Freire, H.L., Iara, \u00cd.H.N., Ribeiro, L.S.R., Gon\u00e7alves, P.A.O., Matta, D.H., and Torres, B.B.J. (2025). Neurological manifestation of canine distemper virus: Increased risk in young Shih Tzu and Lhasa apso with seasonal prevalence in Autumn. Viruses, 17.","DOI":"10.20944\/preprints202504.2531.v1"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Chludzinski, E., Klemens, J., Ciurkiewicz, M., Geffers, R., P\u00f6pperl, P., Stoff, M., Shin, D.-L., Herrler, G., and Beineke, A. (2022). Phenotypic and transcriptional changes of pulmonary immune responses in dogs following canine distemper virus infection. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231710019"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1556\/004.2023.00809","article-title":"Pathological and molecular investigation of canine distemper virus: Phylogenetic analysis of co-circulating genetic lineages in T\u00fcrkiye","volume":"71","author":"Usta","year":"2023","journal-title":"Acta Vet. Hung."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e19344","DOI":"10.1016\/j.heliyon.2023.e19344","article-title":"Establishment and evaluation of a multiplex real-time RT-PCR for quantitative and differential detection of wild-type canine distemper virus from vaccine strains","volume":"9","author":"Sui","year":"2023","journal-title":"Heliyon"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jviromet.2006.05.004","article-title":"Detection of canine distemper virus in dogs by real-time RT-PCR","volume":"136","author":"Elia","year":"2006","journal-title":"J. Virol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jviromet.2013.08.002","article-title":"Detection and differentiation of field and vaccine strains of canine distemper virus using reverse transcription followed by nested real time PCR (RT-NqPCR) and RFLP analysis","volume":"194","author":"Fischer","year":"2013","journal-title":"J. Virol. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e0250521","DOI":"10.1128\/jcm.02505-21","article-title":"Development and validation of a pan-genotypic real-time quantitative reverse transcription-PCR assay to detect canine distemper virus and phocine distemper virus in domestic animals and wildlife","volume":"60","author":"Geiselhardt","year":"2022","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Halecker, S., Bock, S., Beer, M., and Hoffmann, B. (2021). A new molecular detection system for canine distemper virus based on a double-check strategy. Viruses, 13.","DOI":"10.3390\/v13081632"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Iribarnegaray, V., Godi\u00f1o, G., Larra\u00f1aga, C., Yamasaki, K., Verdes, J.M., and Puentes, R. (2024). Droplet digital PCR enhances sensitivity of canine distemper virus detection. Viruses, 16.","DOI":"10.20944\/preprints202409.1390.v1"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Rendon-Marin, S., Higuita-Guti\u00e9rrez, L.F., and Ruiz-Saenz, J. (2024). Safety and immunogenicity of Morbillivirus canis vaccines for domestic and wild animals: A scoping review. Viruses, 16.","DOI":"10.3390\/v16071078"},{"key":"ref_28","first-page":"15","article-title":"Characterization of canine distemper clinical presentations during the epidemic outbreak 2014\u20132018 in Lisbon metropolitan area, Portugal","volume":"116","author":"Machado","year":"2021","journal-title":"Rev. Port. Cien. Vet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101432","DOI":"10.1016\/j.cimid.2020.101432","article-title":"Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach","volume":"69","author":"Rosa","year":"2020","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_30","unstructured":"Dire\u00e7\u00e3o-Geral do Territ\u00f3rio (DGT) (2026, June 16). Carta Administrativa Oficial de Portugal (CAOP), Available online: https:\/\/www.dgterritorio.gov.pt\/atividades\/cartografia\/cartografia-tematica\/caop."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Alfano, F., Lanave, G., Lucibelli, M.G., Miletti, G., D\u2019Alessio, N., Gallo, A., Auriemma, C., Amoroso, M.G., Lucente, M.S., and De Carlo, E. (2022). Canine distemper virus in autochthonous and imported dogs, southern Italy (2014\u20132021). Animals, 12.","DOI":"10.3390\/ani12202852"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Vega-Mari\u00f1o, P., Olson, J., Howitt, B., Criollo, R., Figueroa, L., Orlando, S.A., Cruz, M., and Garcia-Bereguiain, M.A. (2023). A recent distemper virus outbreak in the growing canine populations of Galapagos islands: A persistent threat for the endangered Galapagos sea lion. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1154625"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ke, G.-M., Ho, C.-H., Chiang, M.-J., Sanno-Duanda, B., Chung, C.-S., Lin, M.-Y., Shi, Y.-Y., Yang, M.-H., Tyan, Y.-C., and Liao, P.-C. (2015). Phylodynamic analysis of the canine distemper virus hemagglutinin gene. BMC Vet. Res., 11.","DOI":"10.1186\/s12917-015-0491-9"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Marques, S., Gomes-Neves, E., Baptista, C.S., Pereira, F.R., Alves-Pereira, A., Os\u00f3rio, P., and M\u00fcller, A. (2023). A survey on vaccination and disease occurrence in municipal and non-profit animal shelters in Portugal. Animals, 13.","DOI":"10.3390\/ani13172723"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Owczarczak-Garstecka, S.C., Furtado, T., Graham, T.M., Lloyd, I., Singleton, D.A., Wallis, L., and Westgarth, C. (2022). Impacts of COVID-19 on owner\u2019s veterinary healthcare seeking behavior for dogs with chronic conditions: An exploratory mixed-methods study with a convenience sample. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.902219"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1002\/vms3.790","article-title":"Detection of canine distemper virus in cerebrospinal fluid, whole blood and mucosal specimens of dogs with distemper using RT-PCR and immunochromatographic assays","volume":"8","author":"Sarchahi","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Martinez-Gutierrez, M., and Ruiz-Saenz, J. (2016). Diversity of susceptible hosts in canine distemper virus infection: A systematic review and data synthesis. BMC Vet. Res., 12.","DOI":"10.1186\/s12917-016-0702-z"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Duque-Valencia, J., Sarute, N., Olarte-Castillo, X.A., and Ru\u00edz-S\u00e1enz, J. (2019). Evolution and interspecies transmission of canine distemper virus-an outlook of the diverse evolutionary landscapes of a multi-host virus. Viruses, 11.","DOI":"10.3390\/v11070582"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"155","DOI":"10.26873\/SVR-1909-2024","article-title":"Circulation and presentation of canine distemper virus among various fox species","volume":"61","author":"Silva","year":"2024","journal-title":"Slov. Vet. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e473","DOI":"10.1111\/tbed.14323","article-title":"Canine distemper virus in wildlife in south-western Europe","volume":"69","author":"Oleaga","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Trogu, T., Canziani, S., Salvato, S., Bianchi, A., Bertoletti, I., Gibelli, L.R., Alborali, G.L., Barbieri, I., Gaffuri, A., and Sala, G. (2021). Canine distemper outbreaks in wild carnivores in northern Italy. Viruses, 13.","DOI":"10.3390\/v13010099"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"199043","DOI":"10.1016\/j.virusres.2023.199043","article-title":"An appraisal of gene targets for phylogenetic classification of canine distemper virus: Is the hemagglutinin the best candidate?","volume":"325","author":"Becker","year":"2023","journal-title":"Virus Res."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/734\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T15:59:56Z","timestamp":1783007996000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,2]]},"references-count":42,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["v18070734"],"URL":"https:\/\/doi.org\/10.3390\/v18070734","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,2]]}}}