{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:34:18Z","timestamp":1785418458547,"version":"3.56.0"},"reference-count":53,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32560869"],"award-info":[{"award-number":["32560869"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32503078"],"award-info":[{"award-number":["32503078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32560869"],"award-info":[{"award-number":["32560869"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32503078"],"award-info":[{"award-number":["32503078"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Jiangxi Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["20242BAB23068"],"award-info":[{"award-number":["20242BAB23068"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Jiangxi Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["20232BAB215001"],"award-info":[{"award-number":["20232BAB215001"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus that causes severe diarrhea in piglets, leading to extensive economic losses in the global swine industry. Previous studies have reported that PDCoV can experimentally infect multiple animal species. However, the replication capability of PDCoV in non-natural host cell lines remains largely unknown. Herein, we investigated the effects of exogenous trypsin on PDCoV replication and systematically evaluated the susceptibility of various cell lines derived from human, murine, and feline to PDCoV. The results showed that highly permissive cell lines (H1299, Caco-2, MC-38) supported efficient viral replication without the requirement for exogenous trypsin, and high concentrations of trypsin induced prominent syncytia formation in infected H1299, U251, and Neuro-2a cells. Furthermore, this study also found that trypsin can enhance PDCoV replication in a concentration-dependent manner in semi-permissive cell lines. PDCoV can replicate in multiple cell lines of human, murine, and feline origin. Human cells (H1299, Caco-2) and murine MC-38 are highly susceptible to PDCoV infection, whereas MCF-7, TE-10, and KYSE-410 cells were completely non-permissive. The results also revealed a potential correlation between basal APN expression and cellular susceptibility to PDCoV. Collectively, this study provides a basis for assessing the interspecies transmission and zoonotic risk of PDCoV.<\/jats:p>","DOI":"10.3390\/v18070776","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T08:08:34Z","timestamp":1784189314000},"page":"776","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Susceptibility of Cell Lines from Different Species to Porcine Deltacoronavirus"],"prefix":"10.3390","volume":"18","author":[{"given":"Hui","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoguo","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimin","family":"Chu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nannan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanying","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyang","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Medical and Information Engineering, Gannan Medical University, Ganzhou 341000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8128-1697","authenticated-orcid":false,"given":"Qi","family":"Peng","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.virol.2021.12.006","article-title":"Pathogenicity, infective dose and altered gut microbiota in piglets infected with porcine deltacoronavirus","volume":"567","author":"Li","year":"2022","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.3201\/eid2007.140296","article-title":"Detection and genetic characterization of deltacoronavirus in pigs, Ohio, USA, 2014","volume":"20","author":"Wang","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.3201\/eid2009.140756","article-title":"Porcine coronavirus HKU15 detected in 9 US states, 2014","volume":"20","author":"Wang","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/tbed.12399","article-title":"Newly Emerged Porcine Deltacoronavirus Associated with Diarrhoea in Swine in China: Identification, Prevalence and Full-Length Genome Sequence Analysis","volume":"62","author":"Song","year":"2015","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1111\/tbed.12690","article-title":"Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014\u20132016","volume":"64","author":"Jang","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.meegid.2018.03.030","article-title":"Genetic characterization and pathogenicity of Japanese porcine deltacoronavirus","volume":"61","author":"Suzuki","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"757","DOI":"10.3201\/eid2204.151852","article-title":"Porcine Deltacoronavirus, Thailand, 2015","volume":"22","author":"Janetanakit","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1111\/tbed.12858","article-title":"Herd-level prevalence and incidence of porcine epidemic diarrhoea virus (PEDV) and porcine deltacoronavirus (PDCoV) in swine herds in Ontario, Canada","volume":"65","author":"Ajayi","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/tbed.13339","article-title":"Retrospective study, full-length genome characterization and evaluation of viral infectivity and pathogenicity of chimeric porcine deltacoronavirus detected in Vietnam","volume":"67","author":"Jermsutjarit","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/tbed.12585","article-title":"Different Lineage of Porcine Deltacoronavirus in Thailand, Vietnam and Lao PDR in 2015","volume":"64","author":"Lorsirigool","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_11","first-page":"e1561","article-title":"First isolation and whole genome characterization of porcine deltacoronavirus from pigs in Peru","volume":"69","author":"Hause","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e70108","DOI":"10.1002\/vms3.70108","article-title":"Development and Application of an Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs","volume":"10","year":"2024","journal-title":"Vet. Med. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Tian, Y., Yu, T., Wang, J., Zhang, H., Jian, Y., Li, X., Wang, G., Wang, G., Hu, Y., and Lu, C. (2024). Genetic characterization of the first Deltacoronavirus from wild birds around Qinghai Lake. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1423367"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9997711","DOI":"10.1155\/tbed\/9997711","article-title":"Susceptibility of Ferret and Cat to Porcine Deltacoronavirus: Evidence of Infection in Ferrets But Not Cats","volume":"2025","author":"Meng","year":"2025","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Liang, Q., Zhang, H., Li, B., Ding, Q., Wang, Y., Gao, W., Guo, D., Wei, Z., and Hu, H. (2019). Susceptibility of Chickens to Porcine Deltacoronavirus Infection. Viruses, 11.","DOI":"10.3390\/v11060573"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3201\/eid2602.190346","article-title":"Porcine Deltacoronavirus Infection and Transmission in Poultry, United States(1)","volume":"26","author":"Boley","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1007\/s00705-017-3351-z","article-title":"Calves are susceptible to infection with the newly emerged porcine deltacoronavirus, but not with the swine enteric alphacoronavirus, porcine epidemic diarrhea virus","volume":"162","author":"Jung","year":"2017","journal-title":"Arch. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5723","DOI":"10.1002\/jmv.28048","article-title":"Susceptibility to mice and potential evolutionary characteristics of porcine deltacoronavirus","volume":"94","author":"Zhang","year":"2022","journal-title":"J. Med. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/s41586-021-04111-z","article-title":"Independent infections of porcine deltacoronavirus among Haitian children","volume":"600","author":"Lednicky","year":"2021","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00278-14","DOI":"10.1128\/genomeA.00278-14","article-title":"Full-Length Genome Sequence of Porcine Deltacoronavirus Strain USA\/IA\/2014\/8734","volume":"2","author":"Li","year":"2014","journal-title":"Genome Announc."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Song, Y., Ren, H., Zeng, Q., Yuan, Y., Xia, L., and Wei, Z. (2022). Preparation of a Single-Chain Antibody against Nucleocapsid Protein of Porcine Deltacoronavirus by Phage Display Technology. Viruses, 14.","DOI":"10.3390\/v14040772"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jviromet.2017.08.020","article-title":"Development and application of a recombinant M protein-based indirect ELISA for the detection of porcine deltacoronavirus IgG antibodies","volume":"249","author":"Luo","year":"2017","journal-title":"J. Virol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3988285","DOI":"10.1155\/tbed\/3988285","article-title":"Development and Comparison of Indirect ELISAs for Detecting IgG and IgA Antibodies Against Major Structural Proteins of Porcine Deltacoronavirus with Virus Neutralization as a Benchmark","volume":"2025","author":"Li","year":"2025","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.coviro.2021.08.010","article-title":"Structure of SARS-CoV-2 spike protein","volume":"50","author":"Zhang","year":"2021","journal-title":"Curr. Opin. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1128\/JVI.02615-14","article-title":"Receptor recognition mechanisms of coronaviruses: A decade of structural studies","volume":"89","author":"Li","year":"2015","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1021\/acscentsci.3c00158","article-title":"Host Cell Membrane Capture by the SARS-CoV-2 Spike Protein Fusion Intermediate","volume":"9","author":"Su","year":"2023","journal-title":"ACS Cent. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e00318-18","DOI":"10.1128\/JVI.00318-18","article-title":"Porcine Deltacoronavirus Engages the Transmissible Gastroenteritis Virus Functional Receptor Porcine Aminopeptidase N for Infectious Cellular Entry","volume":"92","author":"Wang","year":"2018","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1038\/s41426-018-0068-3","article-title":"Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection","volume":"7","author":"Zhu","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"11133","DOI":"10.1128\/JVI.00959-09","article-title":"Two-step conformational changes in a coronavirus envelope glycoprotein mediated by receptor binding and proteolysis","volume":"83","author":"Matsuyama","year":"2009","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.molcel.2020.04.022","article-title":"A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells","volume":"78","author":"Hoffmann","year":"2020","journal-title":"Mol. Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"11675","DOI":"10.1038\/s41467-025-66693-w","article-title":"Effect of the S2\u2019 site cleavage on SARS-CoV-2 spike","volume":"16","author":"Shi","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/bs.aivir.2019.08.002","article-title":"Structural insights into coronavirus entry","volume":"105","author":"Tortorici","year":"2019","journal-title":"Adv. Virus Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e02453-20","DOI":"10.1128\/JVI.02453-20","article-title":"The trypsin-enhanced infection of porcine epidemic diarrhea virus is determined by the S2 subunit of the spike glycoprotein","volume":"95","author":"Tan","year":"2021","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e29712","DOI":"10.1002\/jmv.29712","article-title":"TMPRSS13 promotes the cell entry of swine acute diarrhea syndrome coronavirus","volume":"96","author":"Han","year":"2024","journal-title":"J. Med. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1128\/JCM.00031-15","article-title":"Isolation and characterization of porcine deltacoronavirus from pigs with diarrhea in the United States","volume":"53","author":"Hu","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.3201\/eid2007.140571","article-title":"MERS coronavirus in dromedary camel herd, Saudi Arabia","volume":"20","author":"Hemida","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/425915a","article-title":"Virology: SARS virus infection of cats and ferrets","volume":"425","author":"Martina","year":"2003","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e0111821","DOI":"10.1128\/JVI.01118-21","article-title":"Roles of Two Major Domains of the Porcine Deltacoronavirus S1 Subunit in Receptor Binding and Neutralization","volume":"95","author":"Liu","year":"2021","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e70061","DOI":"10.1002\/imt2.70061","article-title":"A gut microbiota-bile acid axis inhibits the infection of an emerging coronavirus by targeting its cellular receptor aminopeptidase N","volume":"4","author":"Zhang","year":"2025","journal-title":"Imeta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1038\/s41396-023-01368-2","article-title":"Comparative susceptibility of SARS-CoV-2, SARS-CoV, and MERS-CoV across mammals","volume":"17","author":"Li","year":"2023","journal-title":"ISME J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2246","DOI":"10.1111\/eva.12997","article-title":"Cross-species transmission of deltacoronavirus and the origin of porcine deltacoronavirus","volume":"13","author":"Ye","year":"2020","journal-title":"Evol. Appl."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1177\/1040638716654200","article-title":"Experimental infection of conventional nursing pigs and their dams with Porcine deltacoronavirus","volume":"28","author":"Loy","year":"2016","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wang, H., Qin, Y., Zhao, W., Yuan, T., Yang, C., Mi, X., Zhao, P., Lu, Y., Lu, B., and Chen, Z. (2021). Genetic Characteristics and Pathogenicity of a Novel Porcine Deltacoronavirus Southeast Asia-Like Strain Found in China. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.701612"},{"key":"ref_44","first-page":"E5135","article-title":"Broad receptor engagement of an emerging global coronavirus may potentiate its diverse cross-species transmissibility","volume":"115","author":"Li","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Fang, P., Zhang, H., Sun, H., Wang, G., Xia, S., Ren, J., Zhang, J., Tian, L., Fang, L., and Xiao, S. (2021). Construction, Characterization and Application of Recombinant Porcine Deltacoronavirus Expressing Nanoluciferase. Viruses, 13.","DOI":"10.3390\/v13101991"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"108660","DOI":"10.1016\/j.vetmic.2020.108660","article-title":"Replicative capacity of porcine deltacoronavirus and porcine epidemic diarrhea virus in primary bovine mesenchymal cells","volume":"244","author":"Jung","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Li, H., Shi, Y., and Zhang, T. (2025). Effects of selenomethionine on intestinal microbiota and its metabolism in mice infected with porcine deltacoronavirus. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1632166"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"110453","DOI":"10.1016\/j.virol.2025.110453","article-title":"Revisiting the roles of trypsin in the productive infection of porcine deltacoronavirus in porcine-derived cells","volume":"604","author":"Xiao","year":"2025","journal-title":"Virology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1080\/22221751.2020.1730245","article-title":"Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner","volume":"9","author":"Yang","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/357420a0","article-title":"Human aminopeptidase N is a receptor for human coronavirus 229E","volume":"357","author":"Yeager","year":"1992","journal-title":"Nature"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1128\/jvi.71.1.734-737.1997","article-title":"Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus","volume":"71","author":"Benbacer","year":"1997","journal-title":"J. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/357417a0","article-title":"Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV","volume":"357","author":"Delmas","year":"1992","journal-title":"Nature"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1038\/s41467-022-29062-5","article-title":"Structures of a deltacoronavirus spike protein bound to porcine and human receptors","volume":"13","author":"Ji","year":"2022","journal-title":"Nat. Commun."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/776\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:22:01Z","timestamp":1784262121000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/7\/776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,15]]},"references-count":53,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["v18070776"],"URL":"https:\/\/doi.org\/10.3390\/v18070776","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,15]]}}}