{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:59:09Z","timestamp":1785560349444,"version":"3.56.0"},"reference-count":50,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"crossref","award":["# 22-15-00230-\u03a0"],"award-info":[{"award-number":["# 22-15-00230-\u03a0"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"crossref"}]},{"award":["# 22-15-00230-\u03a0"],"award-info":[{"award-number":["# 22-15-00230-\u03a0"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The taxonomic status of the H17N10 and H18N11 influenza A viruses isolated from bats remains unclear due to the absence of quantitative classification criteria at this taxonomic level. A total of 3328 representative IAV genomes, encompassing all eight protein-coding segments, were analysed. Various genetic distance-based metrics were assessed at the pairwise level, including intra- and intergroup nucleotide distances, dN\/dS ratios, and transition\/transversion ratios, to facilitate the differentiation of the Alphainfluenzavirus genus into distinct taxa. Pairwise distances for seven of the eight segments (PB2, PB1, PA, NP, M, NA, NS) consistently differentiated the H17\u2013H18 group from H1\u2013H16. Across segments, intergroup nucleotide divergence was consistently above a lower bound of ~25%, with segment-specific values extending to higher levels (up to ~40% in PB2 and PA), while intragroup divergence remained substantially lower. The HA segment did not conform to this pattern, which is consistent with the hypothesis of ancient reassortment. The distribution of pairwise dN\/dS values for the PB2, PB1, PA, and NP segments is evidently bimodal. Intergroup comparisons were consistently higher across all segments, whereas intragroup values remained lower. A similar lower boundary of approximately 0.12 was observed across segments, while the upper range of intergroup values varied by gene. Overall, the results support a consistent gene-specific separation pattern. Previously demonstrated absence of reassortment compatibility between bat viruses (H17\u2013H18) and canonical influenza A (H1\u2013H16) viruses indicates that these lineages have evolved independently over an extended period. These consistent genomic patterns provide support for the hypothesis that H17N10 and H18N11 viruses may represent a separate species within the genus Alphainfluenzavirus.<\/jats:p>","DOI":"10.3390\/v18080838","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:38:54Z","timestamp":1785411534000},"page":"838","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of Quantitative Genetic Distances Supports the Separation of H17N10 and H18N11 Subtypes of Influenza A Virus into a Distinct Species"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9040-1590","authenticated-orcid":false,"given":"Ilya A.","family":"Volkhin","sequence":"first","affiliation":[{"name":"Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2225-3957","authenticated-orcid":false,"given":"Mariia A.","family":"Dashian","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u2018Centre for Strategic Planning and Management of Biomedical Health Risks\u2019 of the Federal Medical and Biological Agency, 119121 Moscow, Russia"},{"name":"Department of Biomedicine, Pirogov Medical University, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander N.","family":"Lukashev","sequence":"additional","affiliation":[{"name":"Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3668-6601","authenticated-orcid":false,"given":"German A.","family":"Shipulin","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u2018Centre for Strategic Planning and Management of Biomedical Health Risks\u2019 of the Federal Medical and Biological Agency, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0789-4601","authenticated-orcid":false,"given":"Andrei A.","family":"Deviatkin","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u2018Centre for Strategic Planning and Management of Biomedical Health Risks\u2019 of the Federal Medical and Biological Agency, 119121 Moscow, Russia"},{"name":"Laboratory of Postgenomic Technologies, Izmerov Research Institute of Occupational Health, 105275 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"D776","DOI":"10.1093\/nar\/gkaf1159","article-title":"Virus taxonomy: The database of the International Committee on Taxonomy of Viruses","volume":"54","author":"Black","year":"2026","journal-title":"Nucleic Acids Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/s41572-018-0002-y","article-title":"Influenza","volume":"4","author":"Krammer","year":"2018","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.tim.2018.05.013","article-title":"Influenza Virus","volume":"26","author":"Hutchinson","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e2530325123","DOI":"10.1073\/pnas.2530325123","article-title":"Efficient replication of influenza D virus in the human airway underscores zoonotic potential","volume":"123","author":"Sanders","year":"2026","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Pleschka, S. (2012). Overview of Influenza Viruses. Swine Influenza, Springer.","DOI":"10.1007\/82_2012_272"},{"key":"ref_6","unstructured":"(2012). Orthomyxoviridae. Virus Taxonomy, Elsevier. Available online: https:\/\/www.sciencedirect.com\/science\/chapter\/edited-volume\/abs\/pii\/B9780123846846000616."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.virol.2018.07.002","article-title":"Molecular footprints of selective pressure in the neuraminidase gene of currently circulating human influenza subtypes and lineages","volume":"522","author":"Correia","year":"2018","journal-title":"Virology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7952","DOI":"10.1038\/ncomms8952","article-title":"Phylodynamics of H1N1\/2009 influenza reveals the transition from host adaptation to immune-driven selection","volume":"6","author":"Su","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6295","DOI":"10.1128\/JVI.02381-05","article-title":"Influenza Virus Evades Innate and Adaptive Immunity via the NS1 Protein","volume":"80","author":"Marukian","year":"2006","journal-title":"J. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Shao, W., Li, X., Goraya, M., Wang, S., and Chen, J.-L. (2017). Evolution of Influenza A Virus by Mutation and Re-Assortment. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18081650"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2225645","DOI":"10.1080\/22221751.2023.2225645","article-title":"Genetic characterization of a new candidate hemagglutinin subtype of influenza A viruses","volume":"12","author":"Fereidouni","year":"2023","journal-title":"Emerg. Microbes Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1038\/nm1210-1389","article-title":"Induction of unnatural immunity: Prospects for a broadly protective universal influenza vaccine","volume":"16","author":"Nabel","year":"2010","journal-title":"Nat. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4269","DOI":"10.1073\/pnas.1116200109","article-title":"A distinct lineage of influenza A virus from bats","volume":"109","author":"Tong","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Tong, S., Zhu, X., Li, Y., Shi, M., Zhang, J., Bourgeois, M., Yang, H., Chen, X., Recuenco, S., and Gomez, J. (2013). New World Bats Harbor Diverse Influenza A Viruses. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003657"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"12797","DOI":"10.1073\/pnas.1608821113","article-title":"Synthetically derived bat influenza A-like viruses reveal a cell type- but not species-specific tropism","volume":"113","author":"Moreira","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e00864-24","DOI":"10.1128\/jvi.00864-24","article-title":"Incompatible packaging signals and impaired protein functions hinder reassortment of bat H17N10 or H18N11 segment 7 with human H1N1 influenza A viruses","volume":"98","author":"Wang","year":"2024","journal-title":"J. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4500","DOI":"10.1038\/s41467-024-48934-6","article-title":"Deciphering bat influenza H18N11 infection dynamics in male Jamaican fruit bats on a single-cell level","volume":"15","author":"Kessler","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Yang, W., Schountz, T., and Ma, W. (2021). Bat Influenza Viruses: Current Status and Perspective. Viruses, 13.","DOI":"10.3390\/v13040547"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Degtyarev, E., Feoktistova, S., Volchkov, P., and Deviatkin, A. (2024). Complex Evolutionary Dynamics of H5N8 Influenza A Viruses Revealed by Comprehensive Reassortment Analysis. Viruses, 16.","DOI":"10.3390\/v16091405"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Feoktistova, S., Sayganova, M., Trutneva, K., Glazova, O., Blagodatski, A.S., Shevkova, L., Navoikova, A., Anisimov, Y., Albert, E., and Mityaeva, O. (2024). Abundant Intra-Subtype Reassortment Revealed in H13N8 Influenza Viruses. Viruses, 16.","DOI":"10.3390\/v16040568"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Feoktistova, S.G., Ivanova, A.O., Degtyarev, E.P., Smirnova, D.I., Volchkov, P.Y., and Deviatkin, A.A. (2024). Phylogenetic Insights into H7Nx Influenza Viruses: Uncovering Reassortment Patterns and Geographic Variability. Viruses, 16.","DOI":"10.3390\/v16111656"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0042-6822(90)90186-U","article-title":"Cocirculation of two distinct evolutionary lineages of influenza type B virus since 1983","volume":"175","author":"Rota","year":"1990","journal-title":"Virology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"344","DOI":"10.3389\/fcimb.2019.00344","article-title":"The Quest for a Truly Universal Influenza Vaccine","volume":"9","author":"Jang","year":"2019","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Koutsakos, M., Parry, R.H., and Wille, M. (2025). Mounting Evidence for an Expanded Host Range of Influenza B Viruses. Viruses, 17.","DOI":"10.3390\/v17121528"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"001806","DOI":"10.1099\/jgv.0.001806","article-title":"Virus classification based on in-depth sequence analyses and development of demarcation criteria using the Betaflexiviridae as a case study","volume":"103","author":"Silva","year":"2022","journal-title":"J. Gen. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.3390\/v4091425","article-title":"Genetics-Based Classification of Filoviruses Calls for Expanded Sampling of Genomic Sequences","volume":"4","author":"Lauber","year":"2012","journal-title":"Viruses"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"13168","DOI":"10.3389\/av.2024.13168","article-title":"Genotypic differentiation and evolutionary dynamics of puumala orthohantavirus (PUUV)","volume":"68","author":"Dashian","year":"2024","journal-title":"Acta Virol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Deviatkin, A.A., Karganova, G.G., Vakulenko, Y.A., and Lukashev, A.N. (2020). TBEV Subtyping in Terms of Genetic Distance. Viruses, 12.","DOI":"10.3390\/v12111240"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Deviatkin, A.A., Aleshina, Y.A., Karganova, G.G., and Lukashev, A.N. (2024). Selection Pressure Profile Suggests Species Criteria among Tick-Borne Orthoflaviviruses. Viruses, 16.","DOI":"10.3390\/v16101554"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1099\/vir.0.000016","article-title":"Methods for virus classification and the challenge of incorporating metagenomic sequence data","volume":"96","author":"Simmonds","year":"2015","journal-title":"J. Gen. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1093\/bioinformatics\/18.1.77","article-title":"Tolerating some redundancy significantly speeds up clustering of large protein databases","volume":"18","author":"Li","year":"2002","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1093\/bioinformatics\/17.3.282","article-title":"Clustering of highly homologous sequences to reduce the size of large protein databases","volume":"17","author":"Li","year":"2001","journal-title":"Bioinformatics"},{"key":"ref_33","first-page":"W5","article-title":"MAFFT-DASH: Integrated protein sequence and structural alignment","volume":"47","author":"Rozewicki","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1093\/bioinformatics\/btq706","article-title":"phangorn: Phylogenetic analysis in R","volume":"27","author":"Schliep","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"W609","DOI":"10.1093\/nar\/gkl315","article-title":"PAL2NAL: Robust conversion of protein sequence alignments into the corresponding codon alignments","volume":"34","author":"Suyama","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Jeffares, D.C., Tomiczek, B., Sojo, V., and dos Reis, M. (2015). A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-Coding Genes in a Genome. Parasite Genomics Protocols, Springer.","DOI":"10.1007\/978-1-4939-1438-8_4"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.1099\/vir.0.2008\/004606-0","article-title":"The multifunctional NS1 protein of influenza A viruses","volume":"89","author":"Hale","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1750-2659.2007.00030.x","article-title":"Non-random reassortment in human influenza A viruses","volume":"2","author":"Rabadan","year":"2008","journal-title":"Influenza Other Respi. Viruses"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1471-2148-14-16","article-title":"Reassortment patterns of avian influenza virus internal segments among different subtypes","volume":"14","author":"Lu","year":"2014","journal-title":"BMC Evol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Villa, M., and L\u00e4ssig, M. (2017). Fitness cost of reassortment in human influenza. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006685"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Nguyen, T.H., Than, V.T., Thanh, H.D., Hung, V.-K., Nguyen, D.T., and Kim, W. (2016). Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0149608"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Nelson, M.I., Viboud, C., Simonsen, L., Bennett, R.T., Griesemer, S.B., St. George, K., Taylor, J., Spiro, D.J., Sengamalay, N.A., and Ghedin, E. (2008). Multiple Reassortment Events in the Evolutionary History of H1N1 Influenza A Virus Since 1918. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000012"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Steel, J., and Lowen, A.C. (2014). Influenza A Virus Reassortment. Influenza Pathogenesis and Control-Volume I, Springer.","DOI":"10.1007\/82_2014_395"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1099\/jgv.0.000715","article-title":"Pathogenicity of modified bat influenza virus with different M genes and its reassortment potential with swine influenza A virus","volume":"98","author":"Yang","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_46","unstructured":"Kuhn, J.H. (2020). Virus Taxonomy. Reference Module in Life Sciences, Elsevier."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.tim.2014.01.010","article-title":"Bat-derived influenza-like viruses H17N10 and H18N11","volume":"22","author":"Wu","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1038\/s41564-019-0517-3","article-title":"Entry of the bat influenza H17N10 virus into mammalian cells is enabled by the MHC class II HLA-DR receptor","volume":"4","author":"Giotis","year":"2019","journal-title":"Nat. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"18635","DOI":"10.1073\/pnas.1215857109","article-title":"The neuraminidase of bat influenza viruses is not a neuraminidase","volume":"109","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3449","DOI":"10.1038\/s41467-024-47635-4","article-title":"Cross-species spill-over potential of the H9N2 bat influenza A virus","volume":"15","author":"Franks","year":"2024","journal-title":"Nat. Commun."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/8\/838\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:35:25Z","timestamp":1785558925000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/8\/838"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":50,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["v18080838"],"URL":"https:\/\/doi.org\/10.3390\/v18080838","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]}}}