{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:05:07Z","timestamp":1786122307698,"version":"build-2736575974"},"reference-count":150,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["BR28712529"],"award-info":[{"award-number":["BR28712529"]}]},{"award":["BR28712529"],"award-info":[{"award-number":["BR28712529"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Orthopoxviruses are zoonotic DNA viruses of continuing public health importance, as highlighted by recent global mpox outbreaks. The high antigenic similarity among orthopoxviruses enables cross-reactive immune responses but complicates the development of serological diagnostic systems for serosurveillance, assessment of previous exposure, evaluation of vaccine-induced immunity, and species differentiation. This review summarizes current knowledge regarding the structure, biological functions, and diagnostic and vaccine potential of major orthopoxvirus recombinant antigens. Particular attention is given to the genomic organization of Orthopoxvirus, differences between intracellular mature virion (IMV) and extracellular enveloped virion (EV) forms, and the characteristics of key antigens, including A29, M1, H3, A35, and B6. Comparative phylogenetic analyses demonstrated a high degree of conservation among structurally important orthopoxvirus proteins while also identifying variable regions potentially relevant for differential diagnostics. Available evidence indicates that IMV-associated proteins are primarily involved in virus neutralization, whereas EV-associated antigens contribute mainly to limiting viral dissemination. Consequently, multicomponent antigen strategies combining IMV- and EV-associated proteins appear to provide the greatest potential for the development of effective serological assays and next-generation subunit vaccines. Overall, rational antigen selection should be based on integrated evaluation of structural, functional, and immunological properties to optimize orthopoxvirus diagnostic and vaccine platforms.<\/jats:p>","DOI":"10.3390\/v18080863","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T09:14:28Z","timestamp":1786094068000},"page":"863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Current Strategies for Selecting Recombinant Orthopoxvirus Proteins for Immunobiological and Diagnostic Applications"],"prefix":"10.3390","volume":"18","author":[{"given":"Aigerim","family":"Zhakypbek","sequence":"first","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"},{"name":"Department of Microbiology, Virology and Immunology, Faculty of Veterinary Science and Zooengineering, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lespek","family":"Kutumbetov","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulnur","family":"Kuzembekova","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Virology and Immunology, Faculty of Veterinary Science and Zooengineering, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamshat","family":"Shorayeva","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurlan","family":"Kozhabergenov","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bekbolat","family":"Usserbayev","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulnur","family":"Nakhanova","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuanysh","family":"Jekebekov","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding 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Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temirlan","family":"Baiseit","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balzhan","family":"Myrzakhmetova","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7954-6246","authenticated-orcid":false,"given":"Olga","family":"Chervyakova","sequence":"additional","affiliation":[{"name":"Research Institute for Biological Safety Problems, National Holding QazBioPharm, Gvardeiskiy 080409, 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Natl. Acad. Sci. USA"},{"key":"ref_2","unstructured":"International Committee on Taxonomy of Viruses (ICTV) (2025, July 30). Virus Taxonomy: 2023 Release. Available online: https:\/\/ictv.global\/taxonomy."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Shchelkunov, S.N. (2013). An increasing danger of zoonotic orthopoxvirus infections. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003756"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"10230","DOI":"10.1128\/JVI.78.19.10230-10237.2004","article-title":"Protective immunity to vaccinia virus induced by vaccination with multiple recombinant outer membrane proteins of intracellular and extracellular virions","volume":"78","author":"Fogg","year":"2004","journal-title":"J. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.4049\/jimmunol.177.4.2552","article-title":"Subunit recombinant vaccine protects against monkeypox","volume":"177","author":"Heraud","year":"2006","journal-title":"J. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3517","DOI":"10.1128\/JVI.01854-07","article-title":"Vaccination of BALB\/c mice with Escherichia coli-expressed vaccinia virus proteins A27L, B5R, and D8L protects mice from lethal vaccinia virus challenge","volume":"82","author":"Berhanu","year":"2008","journal-title":"J. Virol."},{"key":"ref_7","unstructured":"World Health Organization (2025, July 30). Multi-Country Outbreak of Mpox, External Situation Report No. 53, 29 May 2025. Available online: https:\/\/www.who.int\/publications\/m\/item\/multi-country-outbreak-of-mpox--external-situation-report--53---29-may-2025."},{"key":"ref_8","unstructured":"European Centre for Disease Prevention and Control (2025, December 30). Weekly Communicable Disease Threats Report, Week 43, 18\u201324 October 2025. Available online: https:\/\/www.ecdc.europa.eu."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2542","DOI":"10.1128\/JCM.36.9.2542-2547.1998","article-title":"Naturally occurring orthopoxviruses: Potential for recombination with vaccine vectors","volume":"36","author":"Sandvik","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1017\/S0950268899003799","article-title":"A longitudinal study of an endemic disease in its wildlife reservoir: Cowpox and wild rodents","volume":"124","author":"Hazel","year":"2000","journal-title":"Epidemiol. Infect."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"433","DOI":"10.3201\/eid2902.221411","article-title":"Orthopoxvirus infections in rodents, Nigeria, 2018\u20132019","volume":"29","author":"Meseko","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Silva, N.I.O., de Oliveira, J.S., Kroon, E.G., Trindade, G.d.S., and Drumond, B.P. (2021). The wide host range and geographic distribution of zoonotic orthopoxviruses. Viruses, 13.","DOI":"10.3390\/v13010043"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"baaa062","DOI":"10.1093\/database\/baaa062","article-title":"NCBI taxonomy: A comprehensive update on curation, resources and tools","volume":"2020","author":"Schoch","year":"2020","journal-title":"Database"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.3201\/eid2312.162098","article-title":"Fatal outbreak in Tonkean macaques caused by a novel orthopoxvirus, Italy, 2015","volume":"23","author":"Cardeti","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gruber, C.E.M., Giombini, E., Selleri, M., Tausch, S.H., Andrusch, A., Tyshaieva, A., Cardeti, G., Lorenzetti, R., De Marco, L., and Carletti, F. (2018). Whole genome characterization of orthopoxvirus Abatino. Viruses, 10.","DOI":"10.3390\/v10100546"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.3201\/eid2409.171283","article-title":"Novel orthopoxvirus and lethal disease in cat, Italy","volume":"24","author":"Lanave","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Gao, J., Gigante, C., Khmaladze, E., Liu, P., Tang, S., Wilkins, K., Zhao, K., Davidson, W., Nakazawa, Y., and Maghlakelidze, G. (2018). Genome sequences of Akhmeta virus, an Early Divergent Old World Orthopoxvirus. Viruses, 10.","DOI":"10.3390\/v10050252"},{"key":"ref_18","first-page":"8","article-title":"New representatives of the genus Orthopoxvirus","volume":"12","author":"Onishchenko","year":"2023","journal-title":"Infektsionnye Bolezn. Nov. Mneniya Obuchenie"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s13337-013-0145-0","article-title":"Camelpox, an emerging orthopox viral disease","volume":"24","author":"Balamurugan","year":"2013","journal-title":"Indian J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wernery, U., and Kaaden, O.R. (2002). Camelpox. Infectious Diseases in Camelids, Georg Thieme Verlag. [2nd ed.].","DOI":"10.1002\/9780470699058"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1017\/S0950268800058258","article-title":"Serological evidence for the reservoir hosts of cowpox virus in British wildlife","volume":"115","author":"Crouch","year":"1995","journal-title":"Epidemiol. Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1017\/S0950268899002423","article-title":"Cowpox: Reservoir hosts and geographic range","volume":"122","author":"Chantrey","year":"1999","journal-title":"Epidemiol. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Giery\u0144ska, M., Szulc-D\u0105browska, L., Struzik, J., Gregorczyk-Zboroch, K.P., Mielcarska, M.B., Toka, F.N., Schollenberger, A., and Biernacka, Z. (2023). Orthopoxvirus zoonoses. Pathogens, 12.","DOI":"10.3390\/pathogens12030363"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"122","DOI":"10.54117\/ijbcm.v2i4.22","article-title":"Mousepox Virus (Ectromelia Virus), molecular mechanisms, clinical manifestations, and control strategies","volume":"2","author":"Iheukwumere","year":"2025","journal-title":"IPS J. Basic Clin. Med."},{"key":"ref_25","first-page":"360","article-title":"The causative agent of infectious ectromelia","volume":"109","author":"Barnard","year":"1931","journal-title":"Proc. Biol. Sci."},{"key":"ref_26","unstructured":"Africa Centres for Disease Control and Prevention (2025, July 30). Monkeypox. Available online: https:\/\/africacdc.org\/disease\/monkeypox\/."},{"key":"ref_27","unstructured":"World Health Organization (2025, July 30). Monkeypox. Available online: https:\/\/www.who.int\/teams\/blueprint\/monkeypox."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"101157","DOI":"10.1016\/j.onehlt.2025.101157","article-title":"Squirrel reservoirs of monkeypox virus are sister species separated by the Sanaga River (Cameroon), as are the two main viral clades","volume":"21","author":"Curaudeau","year":"2025","journal-title":"One Health"},{"key":"ref_29","unstructured":"Veterian Key (2025, July 30). Poxvirus Infections. Available online: https:\/\/veteriankey.com\/poxvirus-infections\/."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Emerson, G.L., Li, Y., Frace, M.A., Olsen-Rasmussen, M.A., Khristova, M.L., Govil, D., Sammons, S.A., Regnery, R.L., Karem, K.L., and Damon, I.K. (2009). The phylogenetics and ecology of the orthopoxviruses endemic to North America. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0007666"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/j.1365-3164.2006.00553.x","article-title":"Raccoonpox in a Canadian cat","volume":"17","author":"Yager","year":"2006","journal-title":"Vet. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"279","DOI":"10.7589\/0090-3558-10.3.279","article-title":"Isolation of viruses from wild mammals in West Africa, 1966\u20131970","volume":"10","author":"Kemp","year":"1974","journal-title":"J. Wildl. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.meegid.2013.10.014","article-title":"Poxviruses and the evolution of host range and virulence","volume":"21","author":"Haller","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/s42003-025-07746-0","article-title":"Viral genomic features predict Orthopoxvirus reservoir hosts","volume":"8","author":"Tseng","year":"2025","journal-title":"Commun. Biol."},{"key":"ref_35","unstructured":"Fenner, F., Henderson, D.A., Arita, I., Jezek, Z., and Ladnyi, I.D. (1988). Smallpox and Its Eradication, World Health Organization."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/S1286-4579(03)00194-1","article-title":"Variola virus immune evasion proteins","volume":"5","author":"Dunlop","year":"2003","journal-title":"Microbes Infect."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Gallardo-Romero, N.F., Drew, C.P., Weiss, S.L., Metcalfe, M.G., Nakazawa, Y.J., Smith, S.K., Emerson, G.L., Hutson, C.L., Salzer, J.S., and Bartlett, J.H. (2012). The pox in the North American backyard: Volepox virus pathogenesis in California mice (Peromyscus californicus). PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0043881"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Gigante, C.M., Gao, J., Tang, S., McCollum, A.M., Wilkins, K., Reynolds, M.G., Davidson, W., McLaughlin, J., Olson, V.A., and Li, Y. (2019). Genome of Alaskapox Virus, a Novel Orthopoxvirus Isolated from Alaska. Viruses, 11.","DOI":"10.3390\/v11080708"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e1200","DOI":"10.1093\/cid\/ciaf497","article-title":"Six Cases of Borealpox and Evidence of a Zoonotic Source: Alaska, 2020\u20132023","volume":"82","author":"Mooring","year":"2025","journal-title":"Clin. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"MacNeill, A.L. (2022). Comparative pathology of zoonotic orthopoxviruses. Pathogens, 11.","DOI":"10.3390\/pathogens11080892"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/j.1348-0421.2005.tb03723.x","article-title":"Preparation of monoclonal antibodies cross-reactive with orthopoxviruses and their application for direct immunofluorescence test","volume":"49","author":"Kitamoto","year":"2005","journal-title":"Microbiol. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Miller, L., Richter, M., Hapke, C., Stern, D., and Nitsche, A. (2011). Genomic expression libraries for the identification of cross-reactive orthopoxvirus antigens. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0021950"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3201\/eid1505.090235","article-title":"Cowpox virus transmission from pet rats to humans, France","volume":"15","author":"Ninove","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"524743","DOI":"10.1155\/2012\/524743","article-title":"Orthopoxvirus genes that mediate disease virulence and host tropism","volume":"2012","author":"Shchelkunov","year":"2012","journal-title":"Adv. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"D817","DOI":"10.1093\/nar\/gkad946","article-title":"ViralZone 2024 provides higher-resolution images and advanced virus-specific resources","volume":"52","author":"Hulo","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/0022-2836(74)90399-4","article-title":"Characterization and localization of the naturally occurring cross-links in vaccinia virus DNA","volume":"88","author":"Geshelin","year":"1974","journal-title":"J. Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1021\/pr070432+","article-title":"Comparative proteomics of human monkeypox and vaccinia intracellular mature and extracellular enveloped virions","volume":"7","author":"Manes","year":"2008","journal-title":"J. Proteome Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1128\/JVI.75.1.303-310.2001","article-title":"The vaccinia virus A33R protein provides a chaperone function for viral membrane localization and tyrosine phosphorylation of the A36R protein","volume":"75","author":"Wolffe","year":"2001","journal-title":"J. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1007\/BF01641272","article-title":"Abstammung, Eigenschaften und Verwendung des attenuierten Vaccinia-Stammes MVA","volume":"3","author":"Mayr","year":"1975","journal-title":"Infection"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"198975","DOI":"10.1016\/j.virusres.2022.198975","article-title":"Evolution of the orthopoxvirus core genome","volume":"323","author":"Molteni","year":"2023","journal-title":"Virus Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"7590","DOI":"10.1128\/JVI.77.13.7590-7600.2003","article-title":"Poxvirus orthologous clusters: Toward defining the minimum essential poxvirus genome","volume":"77","author":"Upton","year":"2003","journal-title":"J. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e01495-21","DOI":"10.1128\/mbio.01495-21","article-title":"Ancient gene capture and recent gene loss shape the evolution of orthopoxvirus-host interaction genes","volume":"12","author":"Senkevich","year":"2021","journal-title":"mBio"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e00576-24","DOI":"10.1128\/msphere.00576-24","article-title":"Exploring the genomic basis of Mpox virus-host transmission and pathogenesis","volume":"9","author":"Young","year":"2024","journal-title":"mSphere"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1099\/0022-1317-45-3-683","article-title":"Conservation and variation in Orthopoxvirus genome structure","volume":"45","author":"Mackett","year":"1979","journal-title":"J. Gen. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.virusres.2006.01.016","article-title":"Poxviruses: Past, present and future","volume":"117","author":"Lefkowitz","year":"2006","journal-title":"Virus Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.cell.2016.09.049","article-title":"Cross-neutralizing and protective human antibody specificities to poxvirus infections","volume":"167","author":"Gilchuk","year":"2016","journal-title":"Cell"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"101098","DOI":"10.1016\/j.lanmic.2025.101098","article-title":"The impact of orthopoxvirus vaccination and Mpox infection on cross-protective immunity: A multicohort observational study","volume":"6","author":"Crandell","year":"2025","journal-title":"Lancet Microbe"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/BF01246382","article-title":"Experimental studies on transformation of the variola virus into the vaccinia virus","volume":"12","author":"Herrlich","year":"1963","journal-title":"Arch. Gesamte Virusforsch."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"12503","DOI":"10.1038\/s41598-025-96755-4","article-title":"Multivalent vaccine candidate from conserved immunogenic peptides in entry or exit proteins of Orthopoxvirus genus","volume":"15","author":"Silva","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"eaeb3840","DOI":"10.1126\/scitranslmed.aeb3840","article-title":"Antigen-agnostic identification of poxvirus broadly neutralizing antibodies targeting OPG153","volume":"17","author":"Paciello","year":"2025","journal-title":"Sci. Transl. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"6627","DOI":"10.1016\/j.vaccine.2010.07.030","article-title":"A protein-based smallpox vaccine protects non-human primates from a lethal monkeypox virus challenge","volume":"28","author":"Buchman","year":"2010","journal-title":"Vaccine"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"257","DOI":"10.15585\/mmwr.mm6510a2","article-title":"Use of Vaccinia Virus Smallpox Vaccine in Laboratory and Health Care Personnel at Risk for Occupational Exposure to Orthopoxviruses: Recommendations of the Advisory Committee on Immunization Practices (ACIP), 2015","volume":"65","author":"Petersen","year":"2016","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"8134","DOI":"10.1038\/s41467-023-43299-8","article-title":"Quantitative proteomics defines mechanisms of antiviral defence and cell death during modified vaccinia Ankara infection","volume":"14","author":"Albarnaz","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1128\/JVI.78.9.4433-4443.2004","article-title":"Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox","volume":"78","author":"Hooper","year":"2004","journal-title":"J. Virol."},{"key":"ref_66","first-page":"214","article-title":"Monkeypox virus quadrivalent mRNA vaccine induces antibody responses and cellular immunity and protects mice against vaccinia virus","volume":"8","author":"Sang","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"105392","DOI":"10.1016\/j.ebiom.2024.105392","article-title":"Single-chain A35R\u2013M1R\u2013B6R trivalent mRNA vaccines protect mice against both mpox virus and vaccinia virus","volume":"109","author":"Kong","year":"2024","journal-title":"EBioMedicine"},{"key":"ref_68","unstructured":"U.S. Food and Drug Administration (2025, July 30). Key Facts about Vaccines to Prevent Mpox Disease, Available online: https:\/\/www.fda.gov\/vaccines-blood-biologics\/vaccines\/key-facts-about-vaccines-prevent-mpox-disease."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1016\/j.vaccine.2006.12.037","article-title":"Adjuvant-enhanced antibody responses to recombinant proteins correlates with protection of mice and monkeys to orthopoxvirus challenges","volume":"25","author":"Fogg","year":"2007","journal-title":"Vaccine"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1016\/j.vaccine.2006.10.009","article-title":"A protein-based smallpox vaccine protects mice from vaccinia and ectromelia virus challenges when given as a prime and single boost","volume":"25","author":"Xiao","year":"2007","journal-title":"Vaccine"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"eaec0254","DOI":"10.1126\/sciadv.aec0254","article-title":"Cryo-EM Structure of the Vaccinia Virus Entry Fusion Complex Reveals a Multicomponent Fusion Machinery","volume":"12","author":"Lin","year":"2026","journal-title":"Sci. Adv."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Yang, X., Yang, X., Du, S., Hu, C., Yang, X., Wang, X., Hu, X., Rcheulishvili, N., Wang, P.G., and Lin, J. (2023). A subunit vaccine candidate composed of Mpox virus A29L, M1R, A35R, and B6R elicits robust immune response in mice. Vaccines, 11.","DOI":"10.3390\/vaccines11091420"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"126384","DOI":"10.1016\/j.vaccine.2024.126384","article-title":"Systematic evaluation of the induction of efficient neutralizing antibodies by recombinant multicomponent subunit vaccines against monkeypox virus","volume":"42","author":"Tan","year":"2024","journal-title":"Vaccine"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Zhang, L., Liu, C., Yang, C., Xiao, X., Xu, S., Wan, S., Xu, K., Li, Y., and Dai, L. (2025). Multivalent mpox protein nanoparticle vaccines confer cross-protection against orthopoxvirus infection. PLoS Pathog., 21.","DOI":"10.1371\/journal.ppat.1013389"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"4726","DOI":"10.1038\/s41467-025-59826-8","article-title":"Mpox multiprotein virus-like nanoparticle vaccine induces neutralizing and protective antibodies in mice and non-human primates","volume":"16","author":"Belghith","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"5948","DOI":"10.1038\/s41467-023-41587-x","article-title":"Monkeypox virus-infected individuals mount comparable humoral immune responses as smallpox-vaccinated individuals","volume":"14","author":"Otter","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_77","unstructured":"Pan American Health Organization (2025, July 30). Laboratory Guidelines for Mpox. Available online: https:\/\/www.paho.org\/sites\/default\/files\/2024-08\/engpahompxlaboratory-guidelines27aug2024.pdf."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"115004","DOI":"10.1016\/j.jviromet.2024.115004","article-title":"Discordant performance of mpox serological assays","volume":"329","author":"Hunt","year":"2024","journal-title":"J. Virol. Methods"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e29134","DOI":"10.1002\/jmv.29134","article-title":"Development of a novel serological assay for the detection of mpox infection in vaccinated populations","volume":"95","author":"Yates","year":"2023","journal-title":"J. Med. Virol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1089\/vbz.2011.0779","article-title":"Optimization of Peptide-Based ELISA for Serological Diagnostics: A Retrospective Study of Human monkeypox Infection","volume":"12","author":"Dubois","year":"2012","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Riccardo, V., and Pablo, G.-C. (2023). Neutralization determinants on poxviruses. Viruses, 15.","DOI":"10.3390\/v15122396"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1002\/pmic.200600926","article-title":"Proteome-Wide Analysis of the Serological Response to Vaccinia and Smallpox","volume":"7","author":"Davies","year":"2007","journal-title":"Proteomics"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"18","DOI":"10.36233\/0372-9311-643","article-title":"Investigation of protective properties of immunodominant orthopoxvirus proteins using different immunization methods","volume":"102","author":"Zrelkin","year":"2025","journal-title":"J. Microbiol. Epidemiol. Immunobiol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1080\/14760584.2017.1306441","article-title":"Influence of protein fold stability on immunogenicity and its implications for vaccine design","volume":"16","author":"Scheiblhofer","year":"2017","journal-title":"Expert Rev. Vaccines"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"e70614","DOI":"10.1002\/mco2.70614","article-title":"An mRNA vaccine based on antigens from conserved regions of monkeypox virus A35R and M1R with a dimer-like conformation confers protection against both monkeypox virus and vaccinia virus infections in mice","volume":"7","author":"Tang","year":"2026","journal-title":"MedComm"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1586\/14789450.3.3.345","article-title":"Protein Glycosylation in Diverse Cell Systems: Implications for Modification and Analysis of Recombinant Proteins","volume":"3","author":"Brooks","year":"2006","journal-title":"Expert Rev. Proteom."},{"key":"ref_87","first-page":"1680","article-title":"A standardized production pipeline for high profile targets from Mycobacterium tuberculosis","volume":"5","author":"Milewski","year":"2016","journal-title":"SpringerPlus"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/2193-1801-2-12","article-title":"Expression, purification and characterization of a recombinant fusion protein based on the human papillomavirus-16 E7 antigen","volume":"2","author":"Granadillo","year":"2013","journal-title":"SpringerPlus"},{"key":"ref_89","first-page":"69","article-title":"Emerging strategies for monkeypox: Antigen and antibody applications in diagnostics, vaccines, and treatments","volume":"12","author":"Wang","year":"2025","journal-title":"Mil. Med. Res."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Sagdat, K., Batyrkhan, A., and Kanayeva, D. (2024). Exploring monkeypox virus proteins and rapid detection techniques. Front. Cell. Infect. Microbiol., 14.","DOI":"10.3389\/fcimb.2024.1414224"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.tim.2008.07.009","article-title":"Vaccinia virus morphogenesis and dissemination","volume":"16","author":"Roberts","year":"2008","journal-title":"Trends Microbiol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"e01548-25","DOI":"10.1128\/jcm.01548-25","article-title":"Differentiating mpox infection and vaccination using a validated multiplex orthopoxvirus IgG serology assay","volume":"64","author":"Reed","year":"2026","journal-title":"J. Clin. Microbiol."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Stern, D., Pauly, D., Zydek, M., Miller, L., Piesker, J., Laue, M., Lisdat, F., Dorner, M.B., Dorner, B.G., and Nitsche, A. (2016). Development of a genus-specific antigen capture ELISA for Orthopoxviruses\u2014Target selection and optimized screening. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0150110"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Ushkalenko, N., Ersh, A., Sergeev, A., Filatov, P., and Poltavchenko, A. (2022). Evaluation of rapid dot-immunoassay for detection of Orthopoxviruses using laboratory-grown viruses and animal\u2019s clinical specimens. Viruses, 14.","DOI":"10.3390\/v14112580"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"8687","DOI":"10.1128\/JVI.00852-08","article-title":"Vaccinia virus L1 protein is required for cell entry and membrane fusion","volume":"82","author":"Bisht","year":"2008","journal-title":"J. Virol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"4240","DOI":"10.1073\/pnas.0501103102","article-title":"The 1.51-angstrom structure of the poxvirus L1 protein, a target of potent neutralizing antibodies","volume":"102","author":"Su","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"11339","DOI":"10.1128\/JVI.01491-14","article-title":"Potent neutralization of vaccinia virus by divergent murine antibodies targeting a common site of vulnerability in L1 protein","volume":"88","author":"Kaever","year":"2014","journal-title":"J. Virol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1038\/s41564-025-01936-6","article-title":"Structural insights into tecovirimat antiviral activity and poxvirus resistance","volume":"10","author":"Vernuccio","year":"2025","journal-title":"Nat. Microbiol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"3100","DOI":"10.1038\/s41467-025-58180-z","article-title":"Generation and characterization of neutralizing antibodies against M1R and B6R proteins of monkeypox virus","volume":"16","author":"Qu","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"5988","DOI":"10.1128\/jvi.64.12.5988-5996.1990","article-title":"Use of a cell-free system to identify the vaccinia virus L1R gene product as the major late myristylated virion protein M25","volume":"64","author":"Franke","year":"1990","journal-title":"J. Virol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.virol.2020.02.004","article-title":"Particle-specific neutralizing activity of a monoclonal antibody targeting the poxvirus A33 protein reveals differences between cell-associated and extracellular enveloped virions","volume":"544","author":"Mucker","year":"2020","journal-title":"Virology"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1186\/1743-422X-10-229","article-title":"Active vaccination with vaccinia virus A33 protects mice against lethal vaccinia and ectromelia viruses but not against cowpoxvirus; elucidation of the specific adaptive immune response","volume":"10","author":"Paran","year":"2013","journal-title":"Virol. J."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"4732","DOI":"10.1128\/jvi.67.8.4732-4741.1993","article-title":"Deletion of the vaccinia virus B5R gene encoding a 42-kilodalton membrane glycoprotein inhibits extracellular virus envelope formation and dissemination","volume":"67","author":"Wolffe","year":"1993","journal-title":"J. Virol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"6260","DOI":"10.1128\/JVI.79.10.6260-6271.2005","article-title":"Epitope-mapping studies define two major neutralization sites on the vaccinia virus extracellular enveloped virus glycoprotein B5R","volume":"79","author":"Whitbeck","year":"2005","journal-title":"J. Virol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1128\/JVI.01797-08","article-title":"Neutralization of vaccinia virus extracellular enveloped virions in vitro and protection in vivo require complement","volume":"83","author":"Benhnia","year":"2009","journal-title":"J. Virol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"2915","DOI":"10.1099\/0022-1317-83-12-2915","article-title":"The formation and function of extracellular enveloped vaccinia virus","volume":"83","author":"Smith","year":"2002","journal-title":"J. Gen. Virol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1128\/JVI.74.7.3353-3365.2000","article-title":"Vaccinia virus envelope H3L protein binds to cell surface heparan sulfate and is important for intracellular mature virion morphogenesis and virus infection in vitro and in vivo","volume":"74","author":"Lin","year":"2000","journal-title":"J. Virol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"11724","DOI":"10.1128\/JVI.79.18.11724-11733.2005","article-title":"Vaccinia virus H3L envelope protein is a major target of neutralizing antibodies in humans and elicits protection against lethal challenge in mice","volume":"79","author":"Davies","year":"2005","journal-title":"J. Virol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"5020","DOI":"10.1128\/JVI.02933-15","article-title":"The vaccinia virus H3 envelope protein, a major target of neutralizing antibodies, exhibits a glycosyltransferase fold and binds UDP-glucose","volume":"90","author":"Singh","year":"2016","journal-title":"J. Virol."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Khlusevich, Y., Matveev, A., Emelyanova, L., Goncharova, E., Golosova, N., Pereverzev, I., and Tikunova, N. (2022). New p35 (H3L) epitope involved in vaccinia virus neutralization and its deimmunization. Viruses, 14.","DOI":"10.3390\/v14061224"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/s41541-025-01174-1","article-title":"Next-gen novel nanocage-based multivalent vaccine candidate to tackle the rising menace of mpox","volume":"10","author":"Ahuja","year":"2025","journal-title":"npj Vaccines"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"8750","DOI":"10.1128\/JVI.73.10.8750-8761.1999","article-title":"Vaccinia virus envelope D8L protein binds to cell surface chondroitin sulfate and mediates the adsorption of intracellular mature virions to cells","volume":"73","author":"Hsiao","year":"1999","journal-title":"J. Virol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1074\/jbc.M117.814541","article-title":"Structure-function characterization of three human antibodies targeting the vaccinia virus adhesion molecule D8","volume":"293","author":"Matho","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1038\/s44319-023-00040-2","article-title":"The vaccinia chondroitin sulfate binding protein drives host membrane curvature to facilitate fusion","volume":"25","author":"Pokorny","year":"2024","journal-title":"EMBO Rep."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Diesterbeck, U.S., Ahsendorf, H.P., Frenzel, A., Sharifi, A.R., Schirrmann, T., and Czerny, C.P. (2021). Characterization of an in vivo neutralizing anti-vaccinia virus D8 single-chain fragment variable (scFv) from a human anti-vaccinia virus-specific recombinant library. Vaccines, 9.","DOI":"10.3390\/vaccines9111308"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"5910","DOI":"10.1128\/jvi.65.11.5910-5920.1991","article-title":"Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-dalton outer envelope protein","volume":"65","author":"Blasco","year":"1991","journal-title":"J. Virol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1128\/JVI.72.2.1577-1585.1998","article-title":"A27L protein mediates vaccinia virus interaction with cell surface heparan sulfate","volume":"72","author":"Chung","year":"1998","journal-title":"J. Virol."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"10126","DOI":"10.1128\/JVI.72.12.10126-10137.1998","article-title":"The vaccinia virus 14-kilodalton (A27L) fusion protein forms a triple coiled-coil structure and interacts with the 21-kilodalton (A17L) virus membrane protein through a C-terminal alpha-helix","volume":"72","author":"Rivas","year":"1998","journal-title":"J. Virol."},{"key":"ref_119","first-page":"47","article-title":"The vaccinia virus A27L protein is needed for the microtubule-dependent transport of intracellular mature virus particles","volume":"81","author":"Sanderson","year":"2000","journal-title":"J. Gen. Virol."},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Chang, T.-H., Chang, S.-J., Hsieh, F.-L., Ko, T.-P., Lin, C.-T., Ho, M.-R., Wang, I., Hsu, S.-T.D., Guo, R.-T., and Chang, W. (2013). Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003563"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"18572","DOI":"10.1073\/pnas.0509239102","article-title":"Poxvirus multiprotein entry-fusion complex","volume":"102","author":"Senkevich","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.virol.2008.08.009","article-title":"Vaccinia virus entry\/fusion complex subunit A28 is a target of neutralizing and protective antibodies","volume":"380","author":"Nelson","year":"2008","journal-title":"Virology"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"688","DOI":"10.3390\/v4050688","article-title":"Poxvirus cell entry: How many proteins does it take?","volume":"4","author":"Moss","year":"2012","journal-title":"Viruses"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"7528","DOI":"10.1128\/JVI.75.16.7528-7542.2001","article-title":"Vaccinia virus F13L protein with a conserved phospholipase catalytic motif induces colocalization of the B5R envelope glycoprotein in post-Golgi vesicles","volume":"75","author":"Husain","year":"2001","journal-title":"J. Virol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.3390\/v2112409","article-title":"Development of ST-246 for treatment of poxvirus infections","volume":"2","author":"Jordan","year":"2010","journal-title":"Viruses"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1099\/0022-1317-80-2-425","article-title":"Complementation of P37 (F13L gene) knock-out in vaccinia virus by a cell line expressing the gene constitutively","volume":"80","author":"Borrego","year":"1999","journal-title":"J. Gen. Virol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1128\/jvi.67.3.1688-1692.1993","article-title":"The E3L and K3L vaccinia virus gene products stimulate translation through inhibition of the double-stranded RNA-dependent protein kinase by different mechanisms","volume":"67","author":"Davies","year":"1993","journal-title":"J. Virol."},{"key":"ref_128","doi-asserted-by":"crossref","unstructured":"Park, C., Peng, C., Rahman, M.J., Haller, S.L., Tazi, L., Brennan, G., and Rothenburg, S. (2021). Orthopoxvirus K3 orthologs show virus- and host-specific inhibition of the antiviral protein kinase PKR. PLoS Pathog., 17.","DOI":"10.1371\/journal.ppat.1009183"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S1097-2765(02)00590-7","article-title":"X-ray crystal structure and functional analysis of vaccinia virus K3L reveals molecular determinants for PKR subversion and substrate recognition","volume":"10","author":"Dar","year":"2002","journal-title":"Mol. Cell"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1006\/viro.2002.1479","article-title":"The role of the PKR-inhibitory genes, E3L and K3L, in determining vaccinia virus host range","volume":"299","author":"Langland","year":"2002","journal-title":"Virology"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"4146","DOI":"10.1128\/MCB.17.7.4146","article-title":"Regulation of the protein kinase PKR by the vaccinia virus pseudosubstrate inhibitor K3L is dependent on residues conserved between the K3L protein and the PKR substrate eIF2\u03b1","volume":"17","author":"Silverman","year":"1997","journal-title":"Mol. Cell. Biol."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"4825","DOI":"10.1073\/pnas.89.11.4825","article-title":"The E3L gene of vaccinia virus encodes an inhibitor of the interferon-induced, double-stranded RNA-dependent protein kinase","volume":"89","author":"Chang","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"5251","DOI":"10.1128\/JVI.76.10.5251-5259.2002","article-title":"Blockade of interferon induction and action by the E3L double-stranded RNA binding proteins of vaccinia virus","volume":"76","author":"Xiang","year":"2002","journal-title":"J. Virol."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1128\/jvi.69.1.499-505.1995","article-title":"Reversal of the interferon-sensitive phenotype of a vaccinia virus lacking E3L by expression of the reovirus S4 gene","volume":"69","author":"Beattie","year":"1995","journal-title":"J. Virol."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"6974","DOI":"10.1073\/pnas.0431131100","article-title":"A role for Z-DNA binding in vaccinia virus pathogenesis","volume":"100","author":"Kim","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Guerra, S., C\u00e1ceres, A., Knobeloch, K.-P., Horak, I., and Esteban, M. (2008). Vaccinia virus E3 protein prevents the antiviral action of ISG15. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000096"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"2482702","DOI":"10.1080\/22221751.2025.2482702","article-title":"Rational mpox vaccine design: Immunogenicity and protective effect of individual and multicomponent proteins in mice","volume":"14","author":"Cheng","year":"2025","journal-title":"Emerg. Microbes Infect."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/s41421-025-00771-7","article-title":"Identification of neutralizing nanobodies protecting against poxvirus infection","volume":"11","author":"Yang","year":"2025","journal-title":"Cell Discov."},{"key":"ref_139","doi-asserted-by":"crossref","unstructured":"Yefet, R., Friedel, N., Tamir, H., Polonsky, K., Mor, M., Hagin, D., Sprecher, E., Israely, T., and Freund, N.T. (2022). A35R and H3L are serological and B cell markers for monkeypox infection. medRxiv.","DOI":"10.1101\/2022.08.22.22278946"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"4192","DOI":"10.1128\/JVI.72.5.4192-4204.1998","article-title":"The envelope protein encoded by the A33R gene is required for formation of actin-containing microvilli and efficient cell-to-cell spread of vaccinia virus","volume":"72","author":"Roper","year":"1998","journal-title":"J. Virol."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.1038\/s44321-025-00299-z","article-title":"Anti-M1R\/B6R antibody characterization and bispecific design for enhanced orthopoxvirus protection","volume":"17","author":"Zhao","year":"2025","journal-title":"EMBO Mol. Med."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.1128\/JVI.72.3.2429-2438.1998","article-title":"The extracellular domain of vaccinia virus protein B5R affects plaque phenotype, extracellular enveloped virus formation and virulence","volume":"72","author":"Mathew","year":"1998","journal-title":"J. Virol."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"4660","DOI":"10.1038\/s41467-024-48312-2","article-title":"Two noncompeting human neutralizing antibodies targeting MPXV B6 show protective effects against orthopoxvirus infections","volume":"15","author":"Zhao","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_144","doi-asserted-by":"crossref","unstructured":"Li, J., Li, X., Dong, J., Wei, J., Guo, X., Wang, G., Xu, M., and Zhao, A. (2024). Enhanced immune responses in mice by combining the mpox virus B6R-protein and aluminum hydroxide-CpG vaccine adjuvants. Vaccines, 12.","DOI":"10.3390\/vaccines12070776"},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s00253-025-13466-6","article-title":"Monkeypox virus H3L protein as the target antigen for developing neutralizing antibody and serological assay","volume":"109","author":"Huang","year":"2025","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_146","unstructured":"(2025, January 25). Recombinant Vaccinia Virus Envelope Protein H3 (H3L): Expression and Functional Characteristics. Available online: https:\/\/www.cusabio.com\/."},{"key":"ref_147","doi-asserted-by":"crossref","unstructured":"Tang, D., Liu, X., Lu, J., Fan, H., Xu, X., Sun, K., Wang, R., Li, C., Dan, D., and Du, H. (2023). Recombinant proteins A29L, M1R, A35R, and B6R vaccination protects mice from mpox virus challenge. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1203410"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1007\/s00253-024-13361-6","article-title":"Monkeypox virus A29L protein as the target for specific diagnosis and serological analysis","volume":"108","author":"Liang","year":"2024","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_149","unstructured":"Leadgene Biomedical (2025, December 16). Anti-MPXV A29L IgG Antibody. Available online: https:\/\/www.leadgenebio.com\/anti-mpxv-a29L-igg-antibody-clone-ma01-ldg0147ya.html."},{"key":"ref_150","doi-asserted-by":"crossref","unstructured":"Marchi, S., Piccini, G., Remarque, E.J., Roscia, G., Semplici, B., Cantaloni, P., Guerrini, N., Zannella, R., Coluccio, R., and Benincasa, L. (2025). Correlation between microneutralization test and a multiplexed immunoassay for evaluation of monkeypox and vaccinia virus antibodies before and after smallpox vaccination. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1585284"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/8\/863\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T09:17:25Z","timestamp":1786094245000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/18\/8\/863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,7]]},"references-count":150,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["v18080863"],"URL":"https:\/\/doi.org\/10.3390\/v18080863","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,7]]}}}