{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:30:03Z","timestamp":1787585403295,"version":"build-2736575974"},"reference-count":65,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["25-16-00072"],"award-info":[{"award-number":["25-16-00072"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"award":["25-16-00072"],"award-info":[{"award-number":["25-16-00072"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Rhizogenic Agrobacterium (Rhizobium) spp. are causative agents of hairy root disease (HRD), a major threat to hydroponic crop production worldwide. The use of specific bacteriophages is considered a prospective approach to control the development of HRD in greenhouses. A combination of diverse bacteriophages is a key step to overcome potential phage resistance in the pathogen. In this study, a novel lytic bacteriophage, named Miki, was identified and characterized for its antibacterial potential against a rhizogenic Agrobacterium sp. strain circulating in greenhouses in Central Russia. High-throughput sequencing revealed a 63,458 bp double-stranded DNA genome (G + C content 53%), with 117 predicted coding sequences, considering Miki as a lytic candidate phage for plant protection. Electron microscopy of phage Miki shows a morphology unusual for Agrobacterium phages, and phylogenetic analysis attributes it as a representative of a previously undescribed taxon at least at the genus level. The paper presents a detailed analysis of the genome and structural proteome of phage Miki, including in silico predictions and modeling of receptor-binding proteins, including central and proximal fibers resembling the adsorption apparatus of Escherichia phage T5.<\/jats:p>","DOI":"10.3390\/v18090927","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:49:34Z","timestamp":1787582974000},"page":"927","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Lytic Agrobacterium Bacteriophage Miki Representing a New Genus"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9626-3416","authenticated-orcid":false,"given":"Anna D.","family":"Tokmakova","sequence":"first","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"},{"name":"Moscow Center for Advanced Studies, Kulakova Str. 20, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8069-7087","authenticated-orcid":false,"given":"Anna A.","family":"Lukianova","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail M.","family":"Shneider","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2781-3188","authenticated-orcid":false,"given":"Ilia A.","family":"Putilov","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"},{"name":"Faculty of Biology and Biotechnology, HSE University, Profsoyuznaya Str. 33, Bldg. 4, 117418 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina S.","family":"Elkina","sequence":"additional","affiliation":[{"name":"A.P. Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya Str. 8, Bldg. 2, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria S.","family":"Filatova","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"},{"name":"A.P. Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya Str. 8, Bldg. 2, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0675-1043","authenticated-orcid":false,"given":"Anna D.","family":"Burtseva","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, Kulakova Str. 20, 123592 Moscow, Russia"},{"name":"Bach Institute of Biochemistry, Federal Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp. 33, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8229-189X","authenticated-orcid":false,"given":"Konstantin M.","family":"Boyko","sequence":"additional","affiliation":[{"name":"Bach Institute of Biochemistry, Federal Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp. 33, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5646-538X","authenticated-orcid":false,"given":"Yuliya V.","family":"Mikhailova","sequence":"additional","affiliation":[{"name":"Central Research Institute of Epidemiology, Rospotrebnadzor, Novogireyevskaya Str. 3A, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7409-077X","authenticated-orcid":false,"given":"Andrey A.","family":"Shelenkov","sequence":"additional","affiliation":[{"name":"Central Research Institute of Epidemiology, Rospotrebnadzor, Novogireyevskaya Str. 3A, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1646-9802","authenticated-orcid":false,"given":"Peter V.","family":"Evseev","sequence":"additional","affiliation":[{"name":"Pirogov Russian National Research Medical University, Ostrovityanova 1 Str. 1, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4468-4091","authenticated-orcid":false,"given":"Konstantin A.","family":"Miroshnikov","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Choudhary, M., Bankole, I.A., McDuffee, S.T., Parajuli, A., Poudel, M., Balogh, B., Paret, M.L., and Jones, J.B. 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