{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:20:37Z","timestamp":1788326437595,"version":"build-2803163510"},"reference-count":59,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"crossref","award":["FMFU-2022-0002"],"award-info":[{"award-number":["FMFU-2022-0002"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Klebsiella grimontii is an emerging pathogen associated with multidrug resistance. Although a single recent study reported phages against K. grimontii, their taxonomic diversity and structural characterisation remained limited. Here, we describe the isolation and characterisation of five novel lytic bacteriophages that specifically target K. grimontii strain K15g, originally isolated from a rotting iris rhizome. Phages Silvester, Lyra, Lyris, Mirion, and Helios were isolated from wastewater samples collected in the Moscow region and represent three distinct morphotypes: podovirus (Silvester), siphovirus (Lyra, Lyris, Mirion), and myovirus (Helios). Whole-genome sequencing revealed that Silvester belongs to the genus Przondovirus (family Autographiviridae); Lyra, Lyris, and Mirion are members of the genus Sugarlandvirus (family Demerecviridae); and Helios falls within the genus Slopekvirus (family Straboviridae). All five phages lack genes associated with lysogeny, virulence factors, or antibiotic resistance, indicating their suitability for therapeutic applications. Structural modelling of the receptor-binding proteins revealed diverse architectures of adsorption apparatuses, including a two-depolymerase complex in Silvester, T5-like tail fibres in the Sugarlandvirus phages, and a set of long and short fibres with a structured cell-puncturing device in Helios. This study presents five novel phages active against Klebsiella grimontii and provides a basis for further evaluation of their potential in phage therapy.<\/jats:p>","DOI":"10.3390\/v18090942","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:41:18Z","timestamp":1788266478000},"page":"942","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Genomic Characterisation of Five Novel Lytic Bacteriophages Infecting the Emerging Pathogen Klebsiella grimontii"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9626-3416","authenticated-orcid":false,"given":"Anna","family":"Tokmakova","sequence":"first","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"},{"name":"Moscow Center for Advanced Studies, Kulakova Str. 20, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8069-7087","authenticated-orcid":false,"given":"Anna","family":"Lukianova","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail","family":"Shneider","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2781-3188","authenticated-orcid":false,"given":"Ilia","family":"Putilov","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"},{"name":"Faculty of Biology and Biotechnology, HSE University, Profsoyuznaya Str. 33, Bldg. 4, 117418 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0094-0109","authenticated-orcid":false,"given":"Maria","family":"Filatova","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"},{"name":"A.P. Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), 119048 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0675-1043","authenticated-orcid":false,"given":"Anna","family":"Burtseva","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, Kulakova Str. 20, 123592 Moscow, Russia"},{"name":"Bach Institute of Biochemistry, Federal Research Centre \u201cFundamentals of Biotechnology\u201d, Russian Academy of Sciences, Leninsky Prosp. 33, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8229-189X","authenticated-orcid":false,"given":"Konstantin M.","family":"Boyko","sequence":"additional","affiliation":[{"name":"Bach Institute of Biochemistry, Federal Research Centre \u201cFundamentals of Biotechnology\u201d, Russian Academy of Sciences, Leninsky Prosp. 33, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5646-538X","authenticated-orcid":false,"given":"Yuliya","family":"Mikhailova","sequence":"additional","affiliation":[{"name":"Central Research Institute of Epidemiology, Rospotrebnadzor, Novogireyevskaya Str. 3A, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7409-077X","authenticated-orcid":false,"given":"Andrey","family":"Shelenkov","sequence":"additional","affiliation":[{"name":"Central Research Institute of Epidemiology, Rospotrebnadzor, Novogireyevskaya Str. 3A, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0334-4234","authenticated-orcid":false,"given":"Elizaveta","family":"Popova","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Lomonosov MSU, Leninskie Gory 1, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4468-4091","authenticated-orcid":false,"given":"Konstantin","family":"Miroshnikov","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"},{"name":"Faculty of Biology, Lomonosov MSU, Leninskie Gory 1, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00006-21","DOI":"10.1128\/CMR.00006-21","article-title":"Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence","volume":"35","author":"Yang","year":"2022","journal-title":"Clin. 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