{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:08:04Z","timestamp":1792120084796,"version":"4.4.0"},"reference-count":60,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T00:00:00Z","timestamp":1268092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage \u03c6Ea21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.<\/jats:p>","DOI":"10.3390\/v2030710","type":"journal-article","created":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T11:09:10Z","timestamp":1268132950000},"page":"710-730","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":87,"title":["Complete Genomic Sequence of Bacteriophage Felix O1"],"prefix":"10.3390","volume":"2","author":[{"given":"Jean M.","family":"Whichard","sequence":"first","affiliation":[{"name":"Mailstop G29, Centers for Disease Control and Prevention; 1600 Clifton Road, Atlanta, GA 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee A.","family":"Weigt","sequence":"additional","affiliation":[{"name":"Smithsonian National Institution, National Museum in Natural History, MRC 534, Washington, DC 20560, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas J.","family":"Borris","sequence":"additional","affiliation":[{"name":"Abbot Point of Care, 185 Corkstown Road, Ottawa, ON, K2H 8V4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling Ling","family":"Li","sequence":"additional","affiliation":[{"name":"Pennsylvania State University, Department of Veterinary and Biomedical Sciences, 204 Wartick Laboratory, University Park, PA 16802 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N. Seattle, WA 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Kapur","sequence":"additional","affiliation":[{"name":"Pennsylvania State University, 115 Henning Bldg., University Park, PA 16802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. William","family":"Pierson","sequence":"additional","affiliation":[{"name":"VA-MD Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Duck Pond Drive (0442), Blacksburg, Virginia 24061, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika J.","family":"Lingohr","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Min","family":"She","sequence":"additional","affiliation":[{"name":"Centre for Biologics Research, Health Canada, Room D159, Frederick G. Banting Building 251 Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, ON K1A 0K9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Kropinski","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, Ontario N1G 3W4, Canada"},{"name":"University of Guelph, Department of Molecular and Cellular Biology, Guelph, Ontario N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nammalwar","family":"Sriranganathan","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine and Infectious Disease; 1410 Prices Fork Road; Blacksburg, VA 24061-0342, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2010,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0065-3527(08)60785-X","article-title":"Tailed bacteriophages: the order Caudovirales","volume":"51","author":"Ackermann","year":"1998","journal-title":"Adv. Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ackermann, H.W. (2007). Salmonella Phages Observed in the Electron Microscope. In: Salmonella Methods and Protocols\n\t\t\t\t\t\t\t\t. 213\u2013234.","DOI":"10.1007\/978-1-59745-512-1_11"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Schatten, H., and Eisenstark, A. (2007). Salmonella Methods and Protocols, Humana Press.","DOI":"10.1007\/978-1-59745-512-1"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1186\/1471-2180-8-227","article-title":"Genomic analysis of bacteriophage \u03b534 of Salmonella enterica serovar Anatum (15+)","volume":"8","author":"Villafane","year":"2008","journal-title":"BMC Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6970","DOI":"10.1128\/AEM.01088-08","article-title":"Characterization of a T7-like lytic bacteriophage (phiSG-JL2) of Salmonella enterica serovar gallinarum biovar gallinarum","volume":"74","author":"Kwon","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1128\/JB.01654-07","article-title":"Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1","volume":"190","author":"Pickard","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_7","unstructured":"Ackermann, H.W., and DuBow, M.S. (1987). Viruses of Prokaryotes, CRC Press."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4308","DOI":"10.1136\/bmj.2.4308.127","article-title":"Typing of paratyphoid B bacilli by means of Vi bacteriophage","volume":"2","author":"Felix","year":"1943","journal-title":"Brit. Med. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1128\/am.28.4.618-622.1974","article-title":"Identification of Salmonella with the O-1 bacteriophage","volume":"28","author":"Welkos","year":"1974","journal-title":"Appl. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.1699-0463.1967.tb01313.x","article-title":"Resistance to Felix 0-1 phage in salmonella bacteria","volume":"70","author":"Kallings","year":"1967","journal-title":"Acta Pathol. Microbiol. Scand."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1111\/j.1699-0463.1967.tb01312.x","article-title":"Sensitivity of various salmonella strains to felix 0-1 phage","volume":"70","author":"Kallings","year":"1967","journal-title":"Acta Pathol. Microbiol. Scand."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1099\/00221287-109-1-97","article-title":"Lipopolysaccharide core defects in Salmonella typhimurium mutants which are resistant to Felix O phage but retain smooth character","volume":"109","author":"Hudson","year":"1978","journal-title":"J. Gen. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1099\/00221287-87-1-1","article-title":"Mutations in Salmonella typhimurium conferring resistance to Felix O phage without loss of smooth character","volume":"87","author":"MacPhee","year":"1975","journal-title":"J. Gen. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"220","DOI":"10.4315\/0362-028X-66.2.220","article-title":"Suppression of Salmonella growth by wild-type and large-plaque variants of bacteriophage Felix O1 in liquid culture and on chicken frankfurters","volume":"66","author":"Whichard","year":"2003","journal-title":"J. Food Prot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/aem.45.1.260-264.1983","article-title":"Rapid detection of Salmonella spp. by using Felix-O1 bacteriophage and high-performance liquid chromatography","volume":"45","author":"Hirsh","year":"1983","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Schatten, H., and Eisenstark, A. (2007). Salmonella Methods and Protocols, Humana Press.","DOI":"10.1007\/978-1-59745-512-1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0168-1605(01)00683-3","article-title":"Detection of bacteria using foreign DNA: the development of a bacteriophage reagent for Salmonella","volume":"74","author":"Kuhn","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0168-1605(01)00683-3","article-title":"Detection of bacteria using foreign DNA: The development of a bacteriophage reagent for Salmonella","volume":"74","author":"Kuhn","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1093\/nar\/gkg069","article-title":"REBASE: Restriction enzymes and methyltransferases","volume":"31","author":"Roberts","year":"2003","journal-title":"Nucl. Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/bioinformatics\/btn578","article-title":"DNAPlotter: Circular and linear interactive genome visualization","volume":"25","author":"Carver","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1128\/AEM.02352-08","article-title":"Complete genome of the broad-host-range Erwinia amylovora phage \u03c6Ea21-4 and its relationship to Salmonella phage felix O1","volume":"75","author":"Lehman","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1743-422X-6-41","article-title":"The genome and proteome of a virulent Escherichia coli O157:H7 bacteriophage closely resembling Salmonella phage Felix O1","volume":"6","author":"Villegas","year":"2009","journal-title":"Virology J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/978-1-60327-565-1_6","article-title":"In silico identification of genes in bacteriophage DNA","volume":"2","author":"Kropinski","year":"2009","journal-title":"Bacteriophages Methods and Protocols"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1471-2105-3-12","article-title":"CoreGenes: A computational tool for identifying and cataloging \"core\" genes in a set of small genomes","volume":"3","author":"Zafar","year":"2002","journal-title":"BMC Bioinformatics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1101\/gr.2289704","article-title":"Mauve: Multiple alignment of conserved genomic sequence with rearrangements","volume":"14","author":"Darling","year":"2004","journal-title":"Genome Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1186\/1471-2180-9-224","article-title":"Classification of Myoviridae bacteriophages using protein sequence similarity","volume":"9","author":"Lavigne","year":"2009","journal-title":"BMC Microbiol."},{"key":"ref_27","first-page":"6765","article-title":"Phosphoribosylpyrophosphate synthetase of Escherichia coli","volume":"261","author":"Harlow","year":"1986","journal-title":"Properties of the purified enzyme and primary structure of the prs gene. J.Biol.Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1186\/1471-2164-10-589","article-title":"RNRdb, a curated database of the universal enzyme family ribonucleotide reductase, reveals a high level of misannotation in sequences deposited to Genbank","volume":"10","author":"Lundin","year":"2009","journal-title":"BMC Genomics."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1128\/JB.186.7.1933-1944.2004","article-title":"Complete genomic sequence of the virulent Salmonella bacteriophage SP6","volume":"186","author":"Dobbins","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1016\/j.jmb.2003.11.035","article-title":"Genomic analysis of bacteriophages SP6 and K1-5, an estranged subgroup of the T7 supergroup","volume":"335","author":"Scholl","year":"2004","journal-title":"J. Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0022-2836(83)80282-4","article-title":"Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements","volume":"166","author":"Dunn","year":"1983","journal-title":"J. Mol. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.1099\/mic.0.2008\/021360-0","article-title":"An analysis of initiation codon utilization in the Domain Bacteria - concerns about the quality of bacterial genome annotation","volume":"154","author":"Villegas","year":"2008","journal-title":"Microbiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1093\/nar\/25.5.955","article-title":"tRNAscan-SE: A program for improved detection of transfer RNA genes in genomic sequence","volume":"25","author":"Lowe","year":"1997","journal-title":"Nucl. Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7069","DOI":"10.1128\/JB.186.21.7069-7083.2004","article-title":"Lactobacillus plantarum bacteriophage LP65: A new member of the SPO1-like genus of the family Myoviridae","volume":"186","author":"Dillmann","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.yrtph.2005.08.005","article-title":"Bacteriophage P100 for control of Listeria monocytogenes in foods: Genome sequence, bioinformatic analyses, oral toxicity study, and application","volume":"43","author":"Carlton","year":"2005","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.virol.2004.01.029","article-title":"Genomic organization and molecular analysis of the inducible prophage EJ-1, a mosaic myovirus from an atypical pneumococcus","volume":"322","author":"Romero","year":"2004","journal-title":"Virology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1128\/JB.186.6.1818-1832.2004","article-title":"The pKO2 linear plasmid prophage of Klebsiella oxytoca","volume":"186","author":"Casjens","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref_39","first-page":"21","article-title":"The value of prior knowledge in discovering motifs with MEME","volume":"3","author":"Bailey","year":"1995","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1099\/mic.0.28431-0","article-title":"The structural proteome of Pseudomonas aeruginosa bacteriophage \u03c6KMV","volume":"152","author":"Lavigne","year":"2006","journal-title":"Microbiology"},{"key":"ref_41","first-page":"239","article-title":"Phage proteomics: Applications of mass spectrometry","volume":"2","author":"Clokie","year":"2009","journal-title":"Bacteriophages: Methods and Protocols"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/MMBR.67.1.86-156.2003","article-title":"Bacteriophage T4 genome","volume":"67","author":"Miller","year":"2003","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1073\/pnas.95.4.1692","article-title":"Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SP\u03b2","volume":"95","author":"Lazarevic","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3188","DOI":"10.1128\/JB.187.9.3188-3200.2005","article-title":"The genome of S-PM2, a \"photosynthetic\" T4-type bacteriophage that infects marine Synechococcus strains","volume":"187","author":"Mann","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2862","DOI":"10.1128\/JB.186.9.2862-2871.2004","article-title":"Genome of staphylococcal phage K: A new lineage of Myoviridae infecting gram-positive bacteria with a low G+C content","volume":"186","author":"Coffey","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/S0022-2836(03)00634-X","article-title":"Genome of Xanthomonas oryzae bacteriophage Xp10: An odd T-odd phage","volume":"330","author":"Yuzenkova","year":"2003","journal-title":"J. Mol. Biol."},{"key":"ref_47","unstructured":"Adams, M.D. (1959). Interscience Publishers, Inc."},{"key":"ref_48","unstructured":"Borris, D.J. (2002). Temporal analysis of bacteriophage Felix O1 gene expression. M.Sc.Thesis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: A new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucl. Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1093\/nar\/28.1.292","article-title":"Codon usage tabulated from the international DNA sequence databases: Status for the year 2000","volume":"28","author":"Nakamura","year":"2000","journal-title":"Nucl. Acids Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1006\/jmbi.2000.3836","article-title":"Prediction of transcription terminators in bacterial genomes","volume":"301","author":"Ermolaeva","year":"2000","journal-title":"J. Mol. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"W690","DOI":"10.1093\/nar\/gki445","article-title":"RibEx: A web server for locating riboswitches and other conserved bacterial regulatory elements","volume":"33","author":"Merino","year":"2005","journal-title":"Nucl. Acids Res."},{"key":"ref_53","unstructured":"Sambrook, J., and Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press. [3rd]."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2871","DOI":"10.1021\/ac9810516","article-title":"Role of accurate mass measurement (+\/- 10 ppm) in protein identification strategies employing MS or MS\/MS and database searching","volume":"71","author":"Clauser","year":"1999","journal-title":"Anal. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"408","DOI":"10.4319\/lo.2000.45.2.0408","article-title":"The complete genomic sequence of the marine phage Roseophage SIO1shares homology with nonmarine phages","volume":"45","author":"Rohwer","year":"2000","journal-title":"Limnol. Oceanogr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1128\/JB.188.4.1419-1436.2006","article-title":"The genome of the novel phage Rtp, with a rosette-like tail tip, is homologous to the genome of phage T1","volume":"188","author":"Wietzorrek","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_57","unstructured":"Calendar, R.L. (2006). The Bacteriophages, Oxford University Press. [2nd]."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"6924","DOI":"10.1128\/JB.00831-06","article-title":"Genomic analysis of Pseudomonas aeruginosa phages LKD16 and LKA1: Establishment of the \u03c6KMV subgroup within the T7 supergroup","volume":"188","author":"Ceyssens","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1111\/j.1574-6968.2009.01511.x","article-title":"Sequence analysis of Escherichia coli O157:H7 bacteriophage \u03c6V10 and identification of a phage-encoded immunity protein that modifies the O157 antigen","volume":"292","author":"Perry","year":"2009","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.resmic.2008.03.005","article-title":"Unifying classical and molecular taxonomy-based classification: A rational classification system for the Podoviridae using BLASTP-based tools","volume":"59","author":"Lavigne","year":"2008","journal-title":"Res. Microbiol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/2\/3\/710\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:01:41Z","timestamp":1760220101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/2\/3\/710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,9]]},"references-count":60,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2010,3]]}},"alternative-id":["v2030710"],"URL":"https:\/\/doi.org\/10.3390\/v2030710","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,9]]}}}