{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:36:34Z","timestamp":1791250594854,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T00:00:00Z","timestamp":1268870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Rift Valley fever virus (RVFV) is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs) based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly to a wide array of known and previously unknown chemical inhibitors. This system should be useful for screening for small molecule inhibitors of RVFV replication.<\/jats:p>","DOI":"10.3390\/v2030731","type":"journal-article","created":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T12:28:26Z","timestamp":1268915306000},"page":"731-747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication"],"prefix":"10.3390","volume":"2","author":[{"given":"Mary E.","family":"Piper","sequence":"first","affiliation":[{"name":"Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja R.","family":"Gerrard","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA"},{"name":"Department of Epidemiology, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2010,3,18]]},"reference":[{"key":"ref_1","unstructured":"Knipe , D.M., and Howley, P.M. (2007). Fields Virology, Walters Kluwer-Lippincott Williams and Wilkins."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/0035-9203(79)90004-X","article-title":"The Rift Valley fever epizootic in Egypt 1977\u00e2\u0080\u009378.\n\t\t\t\t\t\t\t\t1. Description of the epizootic and virological studies","volume":"73","author":"Meegan","year":"1979","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1136\/vr.105.6.124","article-title":"An epizootic of Rift Valley fever in Egypt in 1977","volume":"105","author":"Meegan","year":"1979","journal-title":"Vet. Rec."},{"key":"ref_4","first-page":"611","article-title":"Hemorrhagic forms of Rift Valley fever in Mauritania","volume":"82","author":"Philippe","year":"1989","journal-title":"Bull. Soc. Pathol. Exot. Filiales"},{"key":"ref_5","first-page":"115","article-title":"Aedes albopictus: a potential problem in France","volume":"37","author":"Rodhain","year":"1995","journal-title":"Parassitologia"},{"key":"ref_6","first-page":"363","article-title":"Transmission of Rift Valley fever virus by different geographic strains of Culex pipiens in Egypt","volume":"64","author":"Gad","year":"1989","journal-title":"J. Egypt Pub. Health Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4269\/ajtmh.1988.38.440","article-title":"Vector potential of selected North American mosquito species for Rift Valley fever virus","volume":"38","author":"Gargan","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_8","first-page":"4","article-title":"Vector competence tests with Rift Valley fever virus and five South African species of mosquito","volume":"4","author":"Jupp","year":"1988","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1046\/j.1365-2915.2002.00371.x","article-title":"The 2000 epidemic of Rift Valley fever in Saudi Arabia: Mosquito vector studies","volume":"16","author":"Jup","year":"2002","journal-title":"Med. Vet. Entomol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.4269\/ajtmh.1980.29.1405","article-title":"Experimental transmission and field isolation studies implicating Culex pipiens as a vector of Rift Valley fever virus in Egypt","volume":"29","author":"Meegan","year":"1980","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"136","DOI":"10.4269\/ajtmh.1996.54.136","article-title":"Vector competence of Egyptian mosquitoes for Rift Valley fever virus","volume":"54","author":"Turell","year":"1996","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1093\/jmedent\/35.2.132","article-title":"Susceptibility of selected strains of Australian mosquitoes (Diptera: Culicidae) to Rift Valley fever virus","volume":"35","author":"Turell","year":"1998","journal-title":"J. Med. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1086\/378747","article-title":"Rift Valley fever epidemic in Saudi Arabia: Epidemiological, clinical, and laboratory characteristics","volume":"37","author":"Madani","year":"2003","journal-title":"Clin. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.3201\/eid0812.020195","article-title":"Genetic analysis of viruses associated with emergence of Rift Valley fever in Saudi Arabia and Yemen, 2000\u20132001","volume":"8","author":"Shoemaker","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.virol.2007.01.046","article-title":"Rift Valley fever virus lacking NSm proteins retains high virulence in vivo and may provide a model of human delayed onset neurologic disease","volume":"362","author":"Bird","year":"2007","journal-title":"Virology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.virol.2006.09.035","article-title":"The NSm proteins of Rift Valley fever virus are dispensable for maturation, replication and infection","volume":"359","author":"Gerrard","year":"2007","journal-title":"Virology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2933","DOI":"10.1128\/JVI.80.6.2933-2940.2006","article-title":"Rescue of infectious rift valley fever virus entirely from cDNA, analysis of virus lacking the NSs gene, and expression of a foreign gene","volume":"80","author":"Ikegami","year":"2006","journal-title":"J. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"8274","DOI":"10.1128\/JVI.00476-06","article-title":"NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture","volume":"80","author":"Won","year":"2006","journal-title":"J. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1099\/vir.0.81227-0","article-title":"Genetic elements regulating packaging of the Bunyamwera orthobunyavirus genome","volume":"87","author":"Kohl","year":"2006","journal-title":"J. Gen. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11726","DOI":"10.1128\/JVI.78.21.11726-11738.2004","article-title":"Functional analysis of the noncoding regions of the Uukuniemi virus (Bunyaviridae) RNA segments","volume":"78","author":"Flick","year":"2004","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3972","DOI":"10.1128\/jvi.69.7.3972-3979.1995","article-title":"The L protein of Rift Valley fever virus can rescue viral ribonucleoproteins and transcribe synthetic genome-like RNA molecules","volume":"69","author":"Lopez","year":"1995","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"12200","DOI":"10.1128\/JVI.76.23.12200-12210.2002","article-title":"Characterization of the Golgi retention motif of Rift Valley fever virus G(N) glycoprotein","volume":"76","author":"Gerrard","year":"2002","journal-title":"J. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.virol.2006.08.002","article-title":"Synthesis, proteolytic processing and complex formation of N-terminally nested precursor proteins of the Rift Valley fever virus glycoproteins","volume":"357","author":"Gerrard","year":"2007","journal-title":"Virology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0042-6822(88)90174-2","article-title":"Rift Valley fever virus M segment: Cellular localization of M segment-encoded proteins","volume":"166","author":"Wasmoen","year":"1988","journal-title":"Virology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1128\/jvi.58.2.263-270.1986","article-title":"Use of bacterial expression cloning to define the amino acid sequences of antigenic determinants on the G2 glycoprotein of Rift Valley fever virus","volume":"58","author":"Keegan","year":"1986","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/jac\/14.suppl_A.27","article-title":"In-vivo activity of antivirals against exotic RNA viral infections","volume":"14","author":"Canonico","year":"1984","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0166-3542(85)80011-5","article-title":"Efficacy, toxicology and clinical applications of ribavirin against virulent RNA viral infections","volume":"1","author":"Canonico","year":"1985","journal-title":"Antivir. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1128\/AAC.24.3.353","article-title":"Broad-spectrum antiviral activity of 2-beta-D-ribofuranosylselenazole-4-carboxamide, a new antiviral agent","volume":"24","author":"Kirsi","year":"1983","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0166-3542(86)90024-0","article-title":"Prophylaxis of Rift Valley fever with antiviral drugs, immune serum, an interferon inducer, and a macrophage activator","volume":"6","author":"Peters","year":"1986","journal-title":"Antiviral Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4365","DOI":"10.1128\/JVI.02148-08","article-title":"NSs protein of rift valley fever virus induces the specific degradation of the double-stranded RNA-dependent protein kinase","volume":"83","author":"Habjan","year":"2009","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/rmv.483","article-title":"Mechanisms of action of ribavirin against distinct viruses","volume":"16","author":"Graci","year":"2006","journal-title":"Rev. Med. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5328","DOI":"10.1073\/pnas.82.16.5328","article-title":"Actinomycin and DNA transcription","volume":"82","author":"Sobell","year":"1985","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","unstructured":"Carter, W.A. (1973). Selective Inhibitors of Viral Functions, CRC Press."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4572","DOI":"10.1128\/JVI.02106-06","article-title":"Guanidine hydrochloride inhibits mammalian orthoreovirus growth by reversibly blocking the synthesis of double-stranded RNA","volume":"81","author":"Murray","year":"2007","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0162-3109(00)00188-0","article-title":"Mycophenolate mofetil and its mechanisms of action","volume":"47","author":"Allison","year":"2000","journal-title":"Immunopharmacol."},{"key":"ref_36","unstructured":"Ausubel, F.M. (1998). John Wiley &amp; Sons."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e1000287","DOI":"10.1371\/journal.ppat.1000287","article-title":"Rift Valley fever virus NSs protein promotes post-transcriptional\n\t\t\t\t\t\t\t\t\tdownregulation of protein kinase PKR and inhibits eIF2alpha\n\t\t\t\t\t\t\t\t\tphosphorylation","volume":"5","author":"Ikegami","year":"2009","journal-title":"PLoS Pathog."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.virol.2006.07.035","article-title":"Development and characterization of a Rift Valley fever virus cell-cell fusion assay using alphavirus replicon vectors","volume":"356","author":"Filone","year":"2006","journal-title":"Virology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/1743-422X-5-82","article-title":"Rift Valley fever virus structural proteins: Expression, characterization and assembly of recombinant proteins","volume":"5","author":"Liu","year":"2008","journal-title":"Virol. J."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/2\/3\/731\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:01:48Z","timestamp":1760220108000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/2\/3\/731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,18]]},"references-count":39,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2010,3]]}},"alternative-id":["v2030731"],"URL":"https:\/\/doi.org\/10.3390\/v2030731","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,18]]}}}