{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:10:40Z","timestamp":1791205840217,"version":"4.1.0"},"reference-count":69,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T00:00:00Z","timestamp":1415059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP-102712"],"award-info":[{"award-number":["MOP-102712"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Retroviral capsid (CA) cores undergo uncoating during their retrograde transport (toward the nucleus), and\/or after reaching the nuclear membrane. However, whether HIV-1 CA core uncoating is dependent upon its transport is not understood. There is some evidence that HIV-1 cores retrograde transport involves cytoplasmic dynein complexes translocating on microtubules. Here we investigate the role of  dynein-dependent transport in HIV-1 uncoating. To interfere with dynein function, we depleted dynein heavy chain (DHC) using RNA interference, and we over-expressed p50\/dynamitin. In immunofluorescence microscopy experiments, DHC depletion caused an accumulation of CA foci in HIV-1 infected cells. Using a biochemical assay to monitor HIV-1 CA core disassembly in infected cells, we observed an increase in amounts of intact (pelletable) CA cores upon DHC depletion or p50 over-expression. Results from these two complementary assays suggest that inhibiting dynein-mediated transport interferes with HIV-1 uncoating in infected cells, indicating the existence of a functional link between HIV-1 transport and uncoating.<\/jats:p>","DOI":"10.3390\/v6114195","type":"journal-article","created":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T09:41:15Z","timestamp":1415094075000},"page":"4195-4211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Cytoplasmic Dynein Promotes HIV-1 Uncoating"],"prefix":"10.3390","volume":"6","author":[{"given":"Paulina","family":"Pawlica","sequence":"first","affiliation":[{"name":"Laboratory of Retrovirology, Department of Medical Biology and BioMed Research Center, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, 3351 Boulevard des Forges, Trois-Rivi\u00e8res, QC G9A 5H7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Berthoux","sequence":"additional","affiliation":[{"name":"Laboratory of Retrovirology, Department of Medical Biology and BioMed Research Center, Universit\u00e9 du Qu\u00e9bec \u00e0 Trois-Rivi\u00e8res, 3351 Boulevard des Forges, Trois-Rivi\u00e8res, QC G9A 5H7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1093\/emboj\/cdg143","article-title":"Structural organization of authentic, mature HIV-1 virions and cores","volume":"22","author":"Briggs","year":"2003","journal-title":"EMBO J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.jmb.2011.04.021","article-title":"The molecular architecture of HIV","volume":"410","author":"Briggs","year":"2011","journal-title":"J. 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