{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:40:05Z","timestamp":1761489605370,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2015,3,23]],"date-time":"2015-03-23T00:00:00Z","timestamp":1427068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Centre for Research","award":["N N402 430039"],"award-info":[{"award-number":["N N402 430039"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Hematopoietic stem cell transplantation from anti-cytomegalovirus immunoglobulin G (anti-CMV-IgG) positive donors facilitated immunological recovery post-transplant, which may indicate that chronic CMV infection has an effect on the immune system. This can be seen in the recipients after reconstitution with donor lymphocytes. We evaluated the composition of lymphocytes at hematologic recovery in 99 patients with hematologic malignancies post hematopoietic stem cell transplantation (HSCT).  Anti-CMV-IgG seropositivity of the donor was associated with higher proportions of CD4+ (227.963 \u00b1 304.858 \u00d7 106 vs. 102.050 \u00b1 17.247 \u00d7 106 cells\/L, p = 0.009) and CD4+CD25high (3.456 \u00b1 0.436 \u00d7 106 vs. 1.589 \u00b1 0.218 \u00d7 106 cells\/L, p = 0.003) lymphocytes in the blood at hematologic recovery. The latter parameter exerted a diverse influence on the risk of acute graft-versus-host disease (GvHD) if low (1.483 \u00b1 0.360 \u00d7 106 vs. 3.778 \u00b1 0.484 \u00d7 106 cells\/L, p &lt; 0.001) and de novo chronic GvHD (cGvHD) if high (3.778 \u00b1 0.780 \u00d7 106 vs. 2.042 \u00b1 0.261 \u00d7 106 cells\/L, p = 0.041). Higher values of CD4+ lymphocytes in patients who received transplants from anti-CMV-IgG-positive donors translated into a reduced demand for IgG support (23\/63 vs. 19\/33, p = 0.048), and these patients also exhibited reduced susceptibility to cytomegalovirus (CMV), Epstein\u2013Barr virus (EBV) and\/or human herpes 6 virus (HHV6) infection\/reactivation (12\/50 vs. 21\/47, p = 0.032). Finally, high levels (\u00b30.4%) of CD4+CD25high lymphocytes were significantly associated with better  post-transplant survival (56% vs. 38%, four-year survival, p = 0.040). Donors who experience CMV infection\/reactivation provide the recipients with lymphocytes, which readily reinforce the recovery of the transplanted patients\u2019 immune system.<\/jats:p>","DOI":"10.3390\/v7031391","type":"journal-article","created":{"date-parts":[[2015,3,23]],"date-time":"2015-03-23T12:17:00Z","timestamp":1427113020000},"page":"1391-1408","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Anti-CMV-IgG Positivity of Donors Is Beneficial for  alloHSCT Recipients with Respect to the Better Short-Term Immunological Recovery and High Level of  CD4+CD25high Lymphocytes"],"prefix":"10.3390","volume":"7","author":[{"given":"Emilia","family":"Jaskula","sequence":"first","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw 53-114, Poland"},{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Dlubek","sequence":"additional","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw 53-114, Poland"},{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Tarnowska","sequence":"additional","affiliation":[{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janusz","family":"Lange","sequence":"additional","affiliation":[{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Mordak-Domagala","sequence":"additional","affiliation":[{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof","family":"Suchnicki","sequence":"additional","affiliation":[{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariola","family":"Sedzimirska","sequence":"additional","affiliation":[{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Borowik","sequence":"additional","affiliation":[{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylwia","family":"Mizia","sequence":"additional","affiliation":[{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Lange","sequence":"additional","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw 53-114, Poland"},{"name":"Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Wroclaw 53-439, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CVI.00247-06","article-title":"Relationship between HLA polymorphisms and gamma interferon and interleukin-10 cytokine production in healthy individuals after rubella vaccination","volume":"14","author":"Ovsyannikova","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1365-3083.2008.02144.x","article-title":"Effect of HLA genotype or CTLA-4 polymorphism on cytokine response in healthy children","volume":"68","author":"Ilonen","year":"2008","journal-title":"Scand. J. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1742-4933-4-2","article-title":"Role of persistent CMV infection in configuring T cell immunity in the elderly","volume":"4","author":"Vasto","year":"2007","journal-title":"Immun. Ageing"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1089\/jir.1996.16.983","article-title":"HLA and cytkine production","volume":"16","author":"Caruso","year":"1996","journal-title":"J. Interferon Cytokine Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1182\/blood-2013-02-482547","article-title":"High-resolution HLA matching in hematopoietic stem cell transplantation: A retrospective collaborative analysis","volume":"122","author":"Vucinic","year":"2013","journal-title":"Blood"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.trim.2014.04.004","article-title":"HLA epitope based matching for transplantation","volume":"31","author":"Duquesnoy","year":"2014","journal-title":"Transpl. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0074-7696(05)25001-5","article-title":"The role of regulatory T cells in controlling immunologic self-tolerance","volume":"225","author":"Takahashi","year":"2003","journal-title":"Int. Rev. Cytol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"693","DOI":"10.2174\/1566524033479429","article-title":"Naturally arising CD25+CD4+ regulatory T cells in maintaining immunologic self-tolerance and preventing autoimmune disease","volume":"3","author":"Takahashi","year":"2003","journal-title":"Curr. Mol. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1093\/intimm\/dxh122","article-title":"Induction of antigen-specific immunologic tolerance by in vivo and in vitro antigen-specific expansion of naturally arising Foxp3+CD25+CD4+ regulatory T cells","volume":"16","author":"Nishimura","year":"2004","journal-title":"Int. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.4049\/jimmunol.155.3.1151","article-title":"Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases","volume":"155","author":"Sakaguchi","year":"1995","journal-title":"J. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2823","DOI":"10.1182\/blood-2013-02-481788","article-title":"Genome-wide DNA methylation analysis identifies hypomethylated genes regulated by FOXP3 in human regulatory T cells","volume":"122","author":"Zhang","year":"2013","journal-title":"Blood"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1189\/jlb.0503228","article-title":"The role of the combination of IL-2 and TGF-beta or IL-10 in the generation and function of CD4+ CD25+ and CD8+ regulatory T cell subsets","volume":"74","author":"Horwitz","year":"2003","journal-title":"J. Leukoc. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5213","DOI":"10.4049\/jimmunol.172.9.5213","article-title":"Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: The role of IL-2, TGF-beta, and IL-10","volume":"172","author":"Zheng","year":"2004","journal-title":"J. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1084\/jem.20060772","article-title":"CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells","volume":"203","author":"Liu","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1084\/jem.20060468","article-title":"Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells","volume":"203","author":"Seddiki","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1365-2567.2008.02813.x","article-title":"Mechanisms of regulatory T-cell suppression\u2014A diverse arsenal for a moving target","volume":"124","author":"Sojka","year":"2008","journal-title":"Immunology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1093\/intimm\/dxp095","article-title":"Regulatory T cells: How do they suppress immune responses?","volume":"21","author":"Sakaguchi","year":"2009","journal-title":"Int. Immunol."},{"key":"ref_18","first-page":"121","article-title":"Homing in on acute graft vs. host disease: Tissue-specific T regulatory and Th17 cells","volume":"341","author":"Engelhardt","year":"2010","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6519","DOI":"10.4049\/jimmunol.172.11.6519","article-title":"Cutting Edge: IL-2 Is Critically Required for the In Vitro Activation of CD4+CD25+ T Cell Suppressor Function","volume":"172","author":"Thornton","year":"2004","journal-title":"J. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/j.1600-0609.1987.tb00010.x","article-title":"Graft-versus-leukaemia activity associated with cytomegalovirus seropositive bone marrow donors but separated from graft-versus-host disease in allograft recipients with AML","volume":"38","author":"Jacobsen","year":"1987","journal-title":"Eur. J. Haematol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/sj.bmt.1705913","article-title":"Risk factors for acute graft-versus-host disease grades II-IV after reduced intensity conditioning allogeneic stem cell transplantation with unrelated donors: A single centre study","volume":"41","author":"Remberger","year":"2008","journal-title":"Bone Marrow Transplant."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"375075","DOI":"10.1155\/2012\/375075","article-title":"CMV Serostatus of Donor-Recipient Pairs Influences the Risk of CMV Infection\/Reactivation in HSCT Patients","volume":"2012","author":"Jaskula","year":"2012","journal-title":"Bone Marrow Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3273","DOI":"10.1016\/j.transproceed.2010.07.027","article-title":"Lange, a Reactivations of cytomegalovirus, human herpes virus 6, and Epstein-Barr virus differ with respect to risk factors and clinical outcome after hematopoietic stem cell transplantation","volume":"42","author":"Jaskula","year":"2010","journal-title":"Transplant. Proc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1111\/tan.12255","article-title":"IL-10 promoter polymorphisms influence susceptibility to aGvHD and are associated with proportions of CD4+FoxP3+ lymphocytes in blood after hematopoietic stem cell transplantation","volume":"82","author":"Jaskula","year":"2013","journal-title":"Tissue Antigens"},{"key":"ref_25","first-page":"197","article-title":"The impact of aging on memory T cell phenotype and function in the human bone marrow","volume":"91","author":"Landgraf","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"346","DOI":"10.3324\/haematol.2012.072991","article-title":"Donor lymphocyte count and thymic activity predict lymphocyte recovery and outcomes after matched-sibling hematopoietic stem cell transplant","volume":"98","author":"McIver","year":"2013","journal-title":"Haematologica"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1080\/10428190290016827","article-title":"The role of donor age in naive T-cell recovery following allogeneic hematopoietic stem cell transplantation: The younger the better","volume":"43","author":"Azuma","year":"2002","journal-title":"Leuk. Lymphoma"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.3390\/v6062242","article-title":"Controlling cytomegalovirus: Helping the immune system take the lead","volume":"6","author":"Hanley","year":"2014","journal-title":"Viruses"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1093\/cid\/ciu369","article-title":"Editorial commentary: The complexity of latent cytomegalovirus infection in stem cell donors","volume":"59","author":"Snydman","year":"2014","journal-title":"Clin. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1182\/blood-2003-10-3616","article-title":"The impact of cytomegalovirus serostatus of donor and recipient before hematopoietic stem cell transplantation in the era of antiviral prophylaxis and preemptive therapy","volume":"103","author":"Boeckh","year":"2004","journal-title":"Blood"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1093\/cid\/ciu364","article-title":"Donor cytomegalovirus status influences the outcome of allogeneic stem cell transplant: A study by the European group for blood and marrow transplantation","volume":"59","author":"Ljungman","year":"2014","journal-title":"Clin. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1097\/MOH.0000000000000085","article-title":"The role of cytomegalovirus serostatus on outcome of hematopoietic stem cell transplantation","volume":"21","author":"Ljungman","year":"2014","journal-title":"Curr. Opin. Hematol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.1182\/blood-2002-10-3263","article-title":"Donor CMV serologic status and outcome of CMV-seropositive recipients after unrelated donor stem cell transplantation: An EBMT megafile analysis","volume":"102","author":"Ljungman","year":"2003","journal-title":"Blood"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1016\/j.bbmt.2009.06.008","article-title":"Interferon gamma 13-CA-repeat homozygous genotype and a low proportion of CD4+ lymphocytes are independent risk factors for cytomegalovirus reactivation with a high number of copies in hematopoietic stem cell transplantation recipients","volume":"15","author":"Jaskula","year":"2009","journal-title":"Biol. Blood Marrow Transplant."},{"key":"ref_35","unstructured":"CMV IgG Test System. Available online: http:\/\/www.zeusscientific.com\/products\/technology-systems\/elisa\/."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.bbmt.2013.12.558","article-title":"NOD2\/CARD15 single nucleotide polymorphism 13 (3020insC) is associated with risk of sepsis and single nucleotide polymorphism 8 (2104C>T) with herpes viruses reactivation in patients after allogeneic hematopoietic stem cell transplantation","volume":"20","author":"Jaskula","year":"2014","journal-title":"Biol. Blood Marrow Transplant."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.idc.2010.01.008","article-title":"Cytomegalovirus in hematopoietic stem cell transplant recipients","volume":"24","author":"Ljungman","year":"2010","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.hoc.2010.11.011","article-title":"Cytomegalovirus in hematopoietic stem cell transplant recipients","volume":"25","author":"Ljungman","year":"2011","journal-title":"Hematol. Oncol. Clin. N. Am."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/j.1399-3062.2010.00504.x","article-title":"Pre-transplant cytomegalovirus (CMV) serostatus remains the most important determinant of CMV reactivation after allogeneic hematopoietic stem cell transplantation in the era of surveillance and preemptive therapy","volume":"12","author":"George","year":"2010","journal-title":"Transpl. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1097\/TP.0000000000000102","article-title":"Who Is the Best Hematopoietic Stem-Cell Donor for a Male Patient With Acute Leukemia?","volume":"98","author":"Labopin","year":"2014","journal-title":"Transplantation"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3877","DOI":"10.1182\/blood.V100.12.3877","article-title":"Graft-versus-host disease and outcome in HLA-identical sibling transplantations for chronic myeloid leukemia","volume":"100","author":"Gratwohl","year":"2002","journal-title":"Blood"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1182\/blood-2011-06-364265","article-title":"Risk factors for acute GVHD and survival after hematopoietic cell transplantation","volume":"119","author":"Jagasia","year":"2012","journal-title":"Blood"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e1002849","DOI":"10.1371\/journal.ppat.1002849","article-title":"Cytomegalovirus infection impairs immune responses and accentuates T-cell pool changes observed in mice with aging","volume":"8","author":"Brien","year":"2012","journal-title":"PLoS Pathog."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1365-2567.2011.03470.x","article-title":"CD8+ CD28\u2212 and CD8+ CD57+ T cells and their role in health and disease","volume":"134","author":"Strioga","year":"2011","journal-title":"Immunology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e89444","DOI":"10.1371\/journal.pone.0089444","article-title":"Impact of HIV on CD8+ T cell CD57 expression is distinct from that of CMV and aging","volume":"9","author":"Lee","year":"2014","journal-title":"PLoS One"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1093\/infdis\/jit576","article-title":"A novel cytomegalovirus-induced regulatory-type T-cell subset increases in size during older life and links virus-specific immunity to vascular pathology","volume":"209","author":"Terrazzini","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.bbmt.2010.02.026","article-title":"Frequency of CD4+CD25hiFOXP3+ regulatory T cells has diagnostic and prognostic value as a biomarker for acute graft-versus-host-disease","volume":"16","author":"Magenau","year":"2010","journal-title":"Biol. Blood Marrow Transplant."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1111\/j.1365-2257.2006.00825.x","article-title":"The initial phase of graft-versus-host disease is associated with a decrease of CD4+CD25+ regulatory T cells in the peripheral blood of patients after allogeneic stem cell transplantation","volume":"28","author":"Schneider","year":"2006","journal-title":"Clin. Lab. Haematol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1111\/j.1432-2277.2007.00462.x","article-title":"Correlation of the CD4+CD25high T-regulatory cells in recipients and their corresponding donors to acute GVHD","volume":"20","author":"Zhai","year":"2007","journal-title":"Transpl. Int."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.4049\/jimmunol.167.3.1245","article-title":"CD4+CD25high Regulatory Cells in Human Peripheral Blood","volume":"167","author":"Brown","year":"2001","journal-title":"J. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1002\/eji.200526189","article-title":"Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level","volume":"35","author":"Roncador","year":"2005","journal-title":"Eur. J. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.4049\/jimmunol.178.4.2018","article-title":"IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells","volume":"178","author":"Zheng","year":"2007","journal-title":"J. Immunol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1182\/blood-2006-02-004747","article-title":"IL-2 regulates FOXP3 expression in human CD4+CD25+ regulatory T cells through a STAT-dependent mechanism and induces the expansion of these cells in vivo","volume":"108","author":"Zorn","year":"2006","journal-title":"Blood"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/nri3156","article-title":"The role of interleukin-2 during homeostasis and activation of the immune system","volume":"12","author":"Boyman","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1097\/01.TP.0000083894.61333.56","article-title":"Is the cytomegalovirus serologic status always accurate? A comparative analysis of humoral and cellular immunity","volume":"76","author":"Sester","year":"2003","journal-title":"Transplantation"},{"key":"ref_56","first-page":"3542","article-title":"Viral mechanisms of immune evasion","volume":"21","author":"Alcami","year":"2000","journal-title":"Trends Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4723","DOI":"10.9734\/BJMMR\/2014\/10807","article-title":"Infectious-like Spread of an Agent Leading to Increased Medical Admissions and Deaths in Wigan ( England ), during 2011 and 2012","volume":"4","author":"Jones","year":"2014","journal-title":"Br. J. Med. Med. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4486","DOI":"10.1128\/JVI.02425-12","article-title":"Intensity of the humoral response to cytomegalovirus is associated with the phenotypic and functional status of the immune system","volume":"87","year":"2013","journal-title":"J. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1014256417651","article-title":"CD28 function: A balance of costimulatory and regulatory signals","volume":"22","author":"Blueston","year":"2002","journal-title":"J. Clin. Immunol"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.4049\/jimmunol.174.5.3051","article-title":"Alloreactive Memory T Cells Are Responsible for the Persistence of Graft-versus-Host Disease","volume":"174","author":"Zhang","year":"2005","journal-title":"J. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/j.1365-2249.2006.03104.x","article-title":"Activation-associated phenotype of CD3 T cells in acute graft-versus-host disease","volume":"145","author":"Briones","year":"2006","journal-title":"Clin. Exp. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.iac.2009.10.001","article-title":"Pathogenesis and management of graft-versus-host disease","volume":"30","author":"Choi","year":"2010","journal-title":"Immunol. Allergy Clin. N. Am."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/S0140-6736(09)60237-3","article-title":"Graft-versus-host disease","volume":"373","author":"Ferrara","year":"2009","journal-title":"Lancet"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"S58","DOI":"10.1038\/bmt.2008.56","article-title":"Acute GvHD: Pathogenesis and classification","volume":"41","author":"Ball","year":"2008","journal-title":"Bone Marrow Transplant."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1097\/MOT.0b013e32834017ae","article-title":"CD4+ regulatory T cells in solid organ transplantation","volume":"15","author":"Issa","year":"2010","journal-title":"Curr. Opin. Organ Transplant."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1097\/TP.0000000000000202","article-title":"High Proportion of Pretransplantation Activated Regulatory T cells (CD4+CD25highCD62L+CD45RO+) Predicts Acute Rejection in Kidney Transplantation","volume":"98","author":"Boix","year":"2014","journal-title":"Transplantation"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/1743-422X-11-145","article-title":"Mouse Cytomegalovirus infection overrules T regulatory cell suppression on natural killer cells","volume":"9","author":"Lindenberg","year":"2014","journal-title":"Virol J."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1097\/QCO.0b013e328259c33b","article-title":"a Cytomegalovirus-associated allograft rejection in heart transplant patients","volume":"20","author":"Potena","year":"2007","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1157\/13114394","article-title":"Indirect effects of cytomegalovirus infection in solid organ transplant recipients","volume":"26","author":"Caston","year":"2008","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Zorn, E., Kim, H.T., Lee, S.J., Floyd, B.H., Litsa, D., Arumugarajah, S., Bellucci, R., Alyea, E.P., Antin, J.H., and Soiffer, R.J. (2005). Reduced frequency of FOXP3 + CD4 + CD25 + regulatory T cells in patients with chronic graft-versus-host disease. 106, 2903\u20132911.","DOI":"10.1182\/blood-2005-03-1257"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Anderson, B.E., Mcniff, J.M., Matte, C., Athanasiadis, I., Shlomchik, W.D., and Shlomchik, M.J. (2004). Recipient CD4 + T cells that survive irradiation regulate chronic graft-versus-host disease. 104, 1565\u20131573.","DOI":"10.1182\/blood-2004-01-0328"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Rieger, K., Loddenkemper, C., Maul, J., Fietz, T., Wolff, D., Terpe, H., Steiner, B., Berg, E., Miehlke, S., and Bornha, M. (2006). Mucosal FOXP3 + regulatory T cells are numerically deficient in acute and chronic GvHD. 107, 1717\u20131723.","DOI":"10.1182\/blood-2005-06-2529"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/7\/3\/1391\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:43:49Z","timestamp":1760215429000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/7\/3\/1391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,23]]},"references-count":72,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2015,3]]}},"alternative-id":["v7031391"],"URL":"https:\/\/doi.org\/10.3390\/v7031391","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2015,3,23]]}}}