{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:44:45Z","timestamp":1791391485954,"version":"4.3.3"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T00:00:00Z","timestamp":1447200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["AI067380"],"award-info":[{"award-number":["AI067380"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Nhumirim virus (NHUV) is an insect-specific virus that phylogenetically affiliates with dual-host mosquito-borne flaviviruses. Previous in vitro co-infection experiments demonstrated prior or concurrent infection of Aedes albopictus C6\/36 mosquito cells with NHUV resulted in a 10,000-fold reduction in viral production of West Nile virus (WNV). This interference between WNV and NHUV was observed herein in an additional Ae. albopictus mosquito cell line, C7-10. A WNV 2K peptide (V9M) mutant capable of superinfection with a pre-established WNV infection demonstrated a comparable level of interference from NHUV as the parental WNV strain in C6\/36 and C7-10 cells. Culex quinquefasciatus and Culex pipiens mosquitoes intrathoracically inoculated with NHUVandWNV, or solely withWNVas a control, were allowed to extrinsically incubate the viruses up to nine and 14 days, respectively, and transmissibility and replication of WNV was determined. The proportion of Cx. quinquefasciatus mosquitoes capable of transmitting WNV was significantly lower for the WNV\/NHUV group than the WNV control at seven and nine days post inoculation (dpi), while no differences were observed in the Cx. pipiens inoculation group. By dpi nine, a 40% reduction in transmissibility in mosquitoes from the dual inoculation group was observed compared to the WNV-only control. These data indicate the potential that infection of some Culex spp. vectors with NHUV could serve as a barrier for efficient transmissibility of flaviviruses associated with human disease.<\/jats:p>","DOI":"10.3390\/v7112911","type":"journal-article","created":{"date-parts":[[2015,11,12]],"date-time":"2015-11-12T03:46:55Z","timestamp":1447300015000},"page":"5801-5812","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":162,"title":["Potential for Co-Infection of a Mosquito-Specific Flavivirus, Nhumirim Virus, to Block West Nile Virus Transmission in Mosquitoes"],"prefix":"10.3390","volume":"7","author":[{"given":"Silvina","family":"Goenaga","sequence":"first","affiliation":[{"name":"Instituto Nacional de Enfermedades Virales Humanas, Pergamino 2700, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan","family":"Kenney","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Duggal","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Delorey","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Ebel","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Levis","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Virales Humanas, Pergamino 2700, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delia","family":"Enria","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Virales Humanas, Pergamino 2700, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Brault","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,11,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1146\/annurev.mi.44.100190.003245","article-title":"Flavivirus genome organization, expression, and replication","volume":"44","author":"Chambers","year":"1990","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Firth, A.E., and Atkins, J.F. (2009). A conserved predicted pseudoknot in the NS2A-encoding sequence of West Nile and Japanese encephalitis flaviviruses suggests NS1\u2032 may derive from ribosomal frameshifting. Virol. J., 6.","DOI":"10.1186\/1743-422X-6-14"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"11274","DOI":"10.1128\/JVI.05864-11","article-title":"A conserved peptide in West Nile virus NS4A protein contributes to proteolytic processing and is essential for replication","volume":"85","author":"Ambrose","year":"2011","journal-title":"J. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1099\/vir.0.036525-0","article-title":"Molecular evolution of the insect-specific flaviviruses","volume":"93","author":"Cook","year":"2012","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1099\/0022-1317-82-8-1867","article-title":"Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography","volume":"82","author":"Gaunt","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1099\/vir.0.068031-0","article-title":"Characterization of a novel insect-specific flavivirus from Brazil: Potential for inhibition of infection of arthropod cells with medically important flaviviruses","volume":"95","author":"Kenney","year":"2014","journal-title":"J. Gen. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.virol.2012.10.020","article-title":"Isolation and genomic characterization of Chaoyang virus strain ROK144 from Aedes vexans nipponii from the Republic of Korea","volume":"435","author":"Lee","year":"2013","journal-title":"Virology"},{"key":"ref_8","first-page":"769","article-title":"A new virus of Flavivirus: Chaoyang virus isolated in Liaoning province","volume":"26","author":"Wang","year":"2009","journal-title":"Chin. Public Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.3390\/v7041927","article-title":"Insect-Specific Flaviviruses: A Systematic Review of Their Discovery, Host Range, Mode of Transmission, Superinfection Exclusion Potential and Genomic Organization","volume":"7","author":"Blitvich","year":"2015","journal-title":"Viruses"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.1099\/vir.0.056200-0","article-title":"Barkedji virus, a novel mosquito-borne flavivirus identified in Culex perexiguus mosquitoes, Israel, 2011","volume":"94","author":"Kolodziejek","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4462","DOI":"10.1128\/JVI.00014-09","article-title":"A new flavivirus and a new vector: Characterization of a novel flavivirus isolated from uranotaenia mosquitoes from a tropical rain forest","volume":"83","author":"Junglen","year":"2009","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"9532","DOI":"10.1128\/JVI.00529-09","article-title":"Characterization of a novel flavivirus from mosquitoes in northern europe that is related to mosquito-borne flaviviruses of the tropics","volume":"83","author":"Huhtamo","year":"2009","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.virol.2014.07.015","article-title":"Novel flaviviruses from mosquitoes: Mosquito-specific evolutionary lineages within the phylogenetic group of mosquito-borne flaviviruses","volume":"464\u2013465","author":"Huhtamo","year":"2014","journal-title":"Virology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1099\/vir.0.050575-0","article-title":"Characterization of a novel flavivirus isolated from Culex (Melanoconion) ocossa mosquitoes from Iquitos, Peru","volume":"94","author":"Evangelista","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1089\/vbz.2011.0687","article-title":"Novel flaviviruses detected in different species of mosquitoes in Spain","volume":"12","author":"Vazquez","year":"2012","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rizzo, F., Cerutti, F., Ballardini, M., Mosca, A., Vitale, N., Radaelli, M.C., Desiato, R., Prearo, M., Pautasso, A., and Casalone, C. (2014). Molecular characterization of flaviviruses from field-collected mosquitoes in northwestern Italy, 2011\u20132012. Parasites Vectors, 7.","DOI":"10.1186\/1756-3305-7-395"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00705-014-2219-8","article-title":"Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil","volume":"160","author":"Solberg","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7221","DOI":"10.1016\/j.vaccine.2011.07.022","article-title":"The pathogenesis of dengue","volume":"29","author":"Whitehorn","year":"2011","journal-title":"Vaccine"},{"key":"ref_19","first-page":"171","article-title":"Pathogenesis and clinical features of Japanese encephalitis and West Nile virus infections","volume":"267","author":"Solomon","year":"2002","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_20","unstructured":"Van Regenmortel, M.H.V. (2008). Encyclopedia of Virology, Elsevier. [3rd ed.]."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1099\/vir.0.011437-0","article-title":"Tick-borne Encephalitis Virus\u2014A Review of an Emerging Zoonosis","volume":"90","author":"Mansfield","year":"2009","journal-title":"J. Gen. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11765","DOI":"10.1128\/JVI.01205-09","article-title":"Exclusion of West Nile virus superinfection through RNA replication","volume":"83","author":"Zou","year":"2009","journal-title":"J. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.virol.2012.02.016","article-title":"Transmission dynamics of an insect-specific flavivirus in a naturally infected Culex pipiens laboratory colony and effects of co-infection on vector competence for West Nile virus","volume":"427","author":"Bolling","year":"2012","journal-title":"Virology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e56534","DOI":"10.1371\/journal.pone.0056534","article-title":"A new insect-specific flavivirus from northern Australia suppresses replication of West Nile virus and Murray Valley encephalitis virus in co-infected mosquito cells","volume":"8","author":"Yam","year":"2013","journal-title":"PLoS ONE"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e671","DOI":"10.1371\/journal.pntd.0000671","article-title":"Transmission of West Nile virus by Culex quinquefasciatus say infected with Culex Flavivirus Izabal","volume":"4","author":"Kent","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1093\/jme\/tju059","article-title":"Analysis of Mosquito-Borne Flavivirus Superinfection in Culex tritaeniorhynchus (Diptera: Culicidae) Cells Persistently Infected with Culex Flavivirus (Flaviviridae)","volume":"52","author":"Kuwata","year":"2015","journal-title":"J. Med. Entomol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.virol.2014.07.009","article-title":"A positively selected mutation in the WNV 2K peptide confers resistance to superinfection exclusion in vivo","volume":"464C\u2013465C","author":"Campbell","year":"2014","journal-title":"Virology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.4269\/ajtmh.14-0289","article-title":"Genetic Determinants of Differential Oral Infection Phenotypes of West Nile and St. Louis Encephalitis Viruses in Culex spp. Mosquitoes","volume":"91","author":"Maharaj","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0166-0934(98)00003-2","article-title":"Universal diagnostic RT-PCR protocol for arboviruses","volume":"72","author":"Kuno","year":"1998","journal-title":"J. Virol. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3611","DOI":"10.1099\/vir.0.82299-0","article-title":"Avian virulence and thermostable replication of the North American strain of West Nile virus","volume":"87","author":"Kinney","year":"2006","journal-title":"J. Gen. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1603\/ME11043","article-title":"Evidence of efficient transovarial transmission of Culex flavivirus by Culex pipiens (Diptera: Culicidae)","volume":"48","author":"Saiyasombat","year":"2011","journal-title":"J. Med. Entomol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1089\/vbz.2010.0144","article-title":"Culex Flavivirus and West Nile Virus Mosquito Coinfection and Positive Ecological Association in Chicago, United States","volume":"11","author":"Newman","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"169","DOI":"10.4269\/ajtmh.2011.10-0474","article-title":"Insect-Specific Flaviviruses from Culex Mosquitoes in Colorado, with Evidence of Vertical Transmission","volume":"85","author":"Bolling","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1089\/vbz.2008.0016","article-title":"Density-dependent competitive suppression of sylvatic dengue virus by endemic dengue virus in cultured mosquito cells","volume":"8","author":"Pepin","year":"2008","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1126\/science.4048949","article-title":"Evolution of bunyaviruses by genome reassortment in dually infected mosquitoes (Aedes triseriatus)","volume":"230","author":"Beaty","year":"1985","journal-title":"Science"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1099\/0022-1317-70-9-2461","article-title":"Viral interference in the tick, Rhipicephalus appendiculatus. I. Interference to oral superinfection by Thogoto virus","volume":"70","author":"Davies","year":"1989","journal-title":"J. Gen. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1128\/jvi.30.1.45-55.1979","article-title":"Heterologous interference in Aedes albopictus cells infected with alphaviruses","volume":"30","author":"Eaton","year":"1979","journal-title":"J. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.1099\/0022-1317-70-10-2595","article-title":"Asynchronous mixed infection of Culicoides variipennis with bluetongue virus serotypes 10 and 17","volume":"70","author":"Ramig","year":"1989","journal-title":"J. Gen. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"7119","DOI":"10.1128\/jvi.71.9.7119-7123.1997","article-title":"Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus","volume":"71","author":"Karpf","year":"1997","journal-title":"J. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1006\/viro.1997.8492","article-title":"Multiple mechanisms for the inhibition of entry and uncoating of superinfecting Semliki Forest virus","volume":"231","author":"Singh","year":"1997","journal-title":"Virology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"428","DOI":"10.4269\/ajtmh.1988.38.428","article-title":"Interference to oral superinfection of Aedes triseriatus infected with La Crosse virus","volume":"38","author":"Sundin","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e1000502","DOI":"10.1371\/journal.ppat.1000502","article-title":"RNAi targeting of West Nile virus in mosquito midguts promotes virus diversification","volume":"5","author":"Brackney","year":"2009","journal-title":"PLoS Pathog."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e856","DOI":"10.1371\/journal.pntd.0000856","article-title":"C6\/36 Aedes albopictus cells have a dysfunctional antiviral RNA interference response","volume":"4","author":"Brackney","year":"2010","journal-title":"PLoS Negl. Trop. Dis."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/7\/11\/2911\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:51:53Z","timestamp":1760215913000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/7\/11\/2911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,11]]},"references-count":43,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2015,11]]}},"alternative-id":["v7112911"],"URL":"https:\/\/doi.org\/10.3390\/v7112911","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,11]]}}}