{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:55:05Z","timestamp":1782402905632,"version":"3.54.5"},"reference-count":49,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T00:00:00Z","timestamp":1478217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Special Fund for Agro-scientific Research in Public Interest","award":["201203055"],"award-info":[{"award-number":["201203055"]}]},{"name":"Natural Science Foundation of Heilongjiang, China","award":["QC2016042"],"award-info":[{"award-number":["QC2016042"]}]},{"name":"National \u201cTwelfth Five-Year\u201d Plan for Science and Technology Support","award":["2015BAD12B03"],"award-info":[{"award-number":["2015BAD12B03"]}]},{"name":"the Scientific and Technological Research Project of Harbin","award":["2014AB3AN058"],"award-info":[{"award-number":["2014AB3AN058"]}]},{"name":"the Heilongjiang Science Foundation for Young Scientists","award":["QC2015037"],"award-info":[{"award-number":["QC2015037"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Avian leukosis virus subgroup J (ALV-J) is an immunosuppressive virus that causes considerable economic losses to the chicken industry in China. However, there is currently no effective vaccine to prevent ALV-J infection. In order to reduce the losses caused by ALV-J, we constructed two effective ALV-J vaccines by inserting the ALV-J (strain JL093-1) env or gag+env genes into the US2 gene of the Marek\u2019s disease herpesviruses (MDV) by transfection of overlapping fosmid DNAs, creating two recombinant MDVs, rMDV\/ALV-gag+env and rMDV\/ALV-env. Analysis of cultured chicken embryo fibroblasts infected with the rMDVs revealed that Env and Gag were successfully expressed and that there was no difference in growth kinetics in cells infected with rMDVs compared with that of cells infected with the parent MDV. Chickens vaccinated with either rMDV revealed that positive serum antibodies were induced. Both rMDVs also effectively reduced the rate of positive viremia in chicken flocks challenged with ALV-J. The protective effect provided by rMDV\/ALV-env inoculation was slightly stronger than that provided by rMDV\/ALV-gag+env. This represents the first study where a potential rMDV vaccine, expressing ALV-J antigenic genes, has been shown to be effective in the prevention of ALV-J. Our study also opens new avenues for the control of MDV and ALV-J co-infection.<\/jats:p>","DOI":"10.3390\/v8110301","type":"journal-article","created":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T11:18:38Z","timestamp":1478258318000},"page":"301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Recombinant Marek\u2019s Disease Virus as a Vector-Based Vaccine against Avian Leukosis Virus Subgroup J in Chicken"],"prefix":"10.3390","volume":"8","author":[{"given":"Yongzhen","family":"Liu","sequence":"first","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Li","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Gao","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Gao","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhong","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Wang","sequence":"additional","affiliation":[{"name":"Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.2307\/1592529","article-title":"Lesions of bone and bone marrow in myeloid leukosis occurring naturally in adult broiler breeders","volume":"44","author":"Nakamura","year":"2000","journal-title":"Avian Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/03079450310001636237a","article-title":"Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China","volume":"33","author":"Xu","year":"2004","journal-title":"Avian Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1099\/0022-1317-72-4-801","article-title":"A novel subgroup of exogenous avian leukosis virus in chickens","volume":"72","author":"Payne","year":"1991","journal-title":"J. Gen. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1128\/JCM.06179-11","article-title":"Molecular epidemiology of avian leukosis virus subgroup J in layer flocks in China","volume":"50","author":"Gao","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1080\/03079450701332345","article-title":"Epidemiological and pathological studies of subgroup J avian leukosis virus infections in Chinese local \u201cyellow\u201d chickens","volume":"36","author":"Sun","year":"2007","journal-title":"Avian Pathol."},{"key":"ref_6","first-page":"584","article-title":"Emerging of avian leukosis virus subgroup J in a flock of Chinese local breed","volume":"45","author":"Cheng","year":"2005","journal-title":"Wei Sheng Wu Xue Bao"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1016\/j.vaccine.2014.02.091","article-title":"Liposomes containing recombinant gp85 protein vaccine against ALV-J in chickens","volume":"32","author":"Zhang","year":"2014","journal-title":"Vaccine"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"6144","DOI":"10.1016\/j.vaccine.2013.06.058","article-title":"Maternal antibody induced by recombinant gp85 protein vaccine adjuvanted with CpG-ODN protects against ALV-J early infection in chickens","volume":"31","author":"Dou","year":"2013","journal-title":"Vaccine"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.pep.2014.10.007","article-title":"Design and construction of a chimeric multi-epitope gene as an epitope-vaccine strategy against ALV-J","volume":"106","author":"Xu","year":"2015","journal-title":"Protein Expr. Purif."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/nrmicro1382","article-title":"Marek\u2019s disease virus: From miasma to model","volume":"4","author":"Osterrieder","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_11","first-page":"25","article-title":"Pathogenesis of Marek\u2019s disease virus infection","volume":"255","author":"Calnek","year":"2001","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"276","DOI":"10.2307\/1590529","article-title":"Protective efficacy of Marek\u2019s disease virus (MDV) CVI-988 CEF65 clone C against challenge infection with three very virulent MDV strains","volume":"30","author":"Groenendal","year":"1986","journal-title":"Avian Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"108","DOI":"10.2307\/1588905","article-title":"Control of Marek\u2019s disease in the Netherlands. I. Isolation of an avirulent Marek\u2019s disease virus (strain CVI 988) and its use in laboratory vaccination trials","volume":"16","author":"Rispens","year":"1972","journal-title":"Avian Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.1016\/S0264-410X(01)00167-0","article-title":"Protection of chickens from lethal avian influenza A virus infection by live-virus vaccination with infectious laryngotracheitis virus recombinants expressing the hemagglutinin (H5) gene","volume":"19","author":"Luschow","year":"2001","journal-title":"Vaccine"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/S0264-410X(97)80001-1","article-title":"Protection of chickens with or without maternal antibodies against both Marek\u2019s and Newcastle diseases by one-time vaccination with recombinant vaccine of Marek\u2019s disease virus type 1","volume":"16","author":"Sakaguchi","year":"1998","journal-title":"Vaccine"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3217","DOI":"10.1128\/JVI.74.7.3217-3226.2000","article-title":"Development of an Effective Polyvalent Vaccine against both Marek\u2019s and Newcastle Diseases Based on Recombinant Marek\u2019s Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies","volume":"74","author":"Sonoda","year":"2000","journal-title":"J. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s00705-011-1131-8","article-title":"Comparative full-length sequence analysis of Marek\u2019s disease virus vaccine strain 814","volume":"157","author":"Zhang","year":"2012","journal-title":"Arch. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1006\/mthe.2000.0050","article-title":"IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector","volume":"1","author":"Mizuguchi","year":"2000","journal-title":"Mol. Ther. J. Am. Soc. Gene Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.vetmic.2013.04.031","article-title":"Protection of chickens against reticuloendotheliosis virus infection by DNA vaccination","volume":"166","author":"Li","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"345","DOI":"10.2307\/1591417","article-title":"Transfection of chicken embryo fibroblasts with Marek\u2019s disease virus DNA","volume":"34","author":"Morgan","year":"1990","journal-title":"Avian Dis."},{"key":"ref_21","first-page":"13","article-title":"Expression of Capsid Protein p27 Gene of Avian Leucosis Virus and Preparation of Multiclonal Antibodies against Recombinant p27 Protein","volume":"34","author":"Delong","year":"2012","journal-title":"China Poult."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11088","DOI":"10.1128\/JVI.74.23.11088-11098.2000","article-title":"Reconstitution of Marek\u2019s disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant","volume":"74","author":"Schumacher","year":"2000","journal-title":"J. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Cui, H., Gao, H., Cui, X., Zhao, Y., Shi, X., Li, Q., Yan, S., Gao, M., Wang, M., and Liu, C. (2013). Avirulent Marek\u2019s disease virus type 1 strain 814 vectored vaccine expressing avian influenza (AI) virus H5 haemagglutinin induced better protection than turkey herpesvirus vectored AI vaccine. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0053340"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10575","DOI":"10.1128\/JVI.01065-07","article-title":"Horizontal transmission of Marek\u2019s disease virus requires US2, the UL13 protein kinase, and gC","volume":"81","author":"Jarosinski","year":"2007","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0168-1702(98)00110-5","article-title":"The genetic organization and transcriptional analysis of the short unique region in the genome of nononcogenic Marek\u2019s disease virus serotype 2","volume":"58","author":"Jang","year":"1998","journal-title":"Virus Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1128\/jvi.65.3.1584-1588.1991","article-title":"Isolation of a Marek\u2019s disease virus (MDV) recombinant containing the lacZ gene of Escherichia coli stably inserted within the MDV US2 gene","volume":"65","author":"Cantello","year":"1991","journal-title":"J. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1097\/QAI.0b013e3181dc98a3","article-title":"Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV","volume":"54","author":"Hicar","year":"2010","journal-title":"J. Acquir. Immune Defic. Syndr."},{"key":"ref_28","first-page":"251","article-title":"Avian tumor viruses: Persistent and evolving pathogens","volume":"45","author":"Witter","year":"1997","journal-title":"Acta Vet. Hung."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.vetimm.2006.03.014","article-title":"Vaccinal control of Marek\u2019s disease: Current challenges, and future strategies to maximize protection","volume":"112","author":"Baigent","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1006\/viro.1993.1572","article-title":"A cosmid-based system for constructing mutants of herpes simplex virus type 1","volume":"197","author":"Cunningham","year":"1993","journal-title":"Virology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/S0168-9525(00)02015-1","article-title":"Forward with BACs: New tools for herpesvirus genomics","volume":"16","author":"Brune","year":"2000","journal-title":"Trends Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1743-422X-5-19","article-title":"Self-excision of the BAC sequences from the recombinant Marek\u2019s disease virus genome increases replication and pathogenicity","volume":"5","author":"Zhao","year":"2008","journal-title":"Virol. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7056","DOI":"10.1128\/JVI.73.8.7056-7060.1999","article-title":"Systematic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution","volume":"73","author":"Wagner","year":"1999","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2871","DOI":"10.1128\/JVI.01734-09","article-title":"Autoexcision of bacterial artificial chromosome facilitated by terminal repeat-mediated homologous recombination: A novel approach for generating traceless genetic mutants of herpesviruses","volume":"84","author":"Zhou","year":"2010","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"11628","DOI":"10.1128\/JVI.01065-06","article-title":"Hemagglutinin (HA) proteins from H1 and H3 serotypes of influenza A viruses require different antigen designs for the induction of optimal protective antibody responses as studied by codon-optimized HA DNA vaccines","volume":"80","author":"Wang","year":"2006","journal-title":"J. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1016\/j.vaccine.2008.01.046","article-title":"Recombinant Marek\u2019s disease virus (MDV) lacking the Meq oncogene confers protection against challenge with a very virulent plus strain of MDV","volume":"26","author":"Lee","year":"2008","journal-title":"Vaccine"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.antiviral.2007.03.009","article-title":"Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector","volume":"75","author":"Jiang","year":"2007","journal-title":"Antivir. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1089\/10430349950017545","article-title":"Transgene expression in the guinea pig cochlea mediated by a lentivirus-derived gene transfer vector","volume":"10","author":"Han","year":"1999","journal-title":"Hum. Gene Ther."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1099\/vir.0.81498-0","article-title":"Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek\u2019s disease","volume":"87","author":"Baigent","year":"2006","journal-title":"J. Gen. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1006\/viro.1999.9641","article-title":"Protection of Chickens against Very Virulent Infectious Bursal Disease Virus (IBDV) and Marek\u2019s Disease Virus (MDV) with a Recombinant MDV Expressing IBDV VP2","volume":"257","author":"Tsukamoto","year":"1999","journal-title":"Virology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.tibtech.2013.09.002","article-title":"Virus-like particle-based human vaccines: Quality assessment based on structural and functional properties","volume":"31","author":"Zhao","year":"2013","journal-title":"Trends Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.virol.2007.04.033","article-title":"A comparative immunogenicity study of HIV-1 virus-like particles bearing various forms of envelope proteins, particles bearing no envelope and soluble monomeric gp120","volume":"366","author":"Crooks","year":"2007","journal-title":"Virology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.virol.2003.12.017","article-title":"Assembly, structure, and antigenic properties of virus-like particles rich in HIV-1 envelope gp120","volume":"321","author":"Berkower","year":"2004","journal-title":"Virology"},{"key":"ref_44","first-page":"200","article-title":"Epidemiological Study of Avian Leukemia in Different Import Poultry Strains from 2009 to 2010 in Some Areas of China","volume":"39","author":"Zhang","year":"2012","journal-title":"China Anim. Husb. Vet. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.jviromet.2012.11.027","article-title":"Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen","volume":"187","author":"Galvis","year":"2013","journal-title":"J. Virol. Methods"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"8257","DOI":"10.1016\/j.vaccine.2011.08.115","article-title":"Recombinant herpesvirus of turkeys as a vector-based vaccine against highly pathogenic H7N1 avian influenza and Marek\u2019s disease","volume":"29","author":"Li","year":"2011","journal-title":"Vaccine"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"391","DOI":"10.2307\/1592636","article-title":"Isolation and some characteristics of a subgroup J-like avian leukosis virus associated with myeloid leukosis in meat-type chickens in the United States","volume":"43","author":"Fadly","year":"1999","journal-title":"Avian Dis."},{"key":"ref_48","unstructured":"Tong, K. (1984). Study on the Immunization of Chickens against Marek\u2019s Disease\u2014Report on a Naturally Avirulent Vaccine Strain of Marek\u2019 Disease Herpesvirus. Chin. J. Anim. Vet. Sci."},{"key":"ref_49","first-page":"294","article-title":"Sequencing analysis of repeat regions of Marek\u2019s disease virus vaccine strain 814","volume":"32","author":"Zhang","year":"2010","journal-title":"Chin. J. Prev. Vet. Med."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/8\/11\/301\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:34:48Z","timestamp":1760211288000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/8\/11\/301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,4]]},"references-count":49,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2016,11]]}},"alternative-id":["v8110301"],"URL":"https:\/\/doi.org\/10.3390\/v8110301","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,4]]}}}