{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T06:06:33Z","timestamp":1768025193035,"version":"3.49.0"},"reference-count":29,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T00:00:00Z","timestamp":1480636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31500125"],"award-info":[{"award-number":["31500125"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81361120385"],"award-info":[{"award-number":["81361120385"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Reactivated varicella-zoster virus (VZV), which lies latent in the dorsal root ganglions and cranial nerves before its reactivation, is capable of causing herpes zoster (HZ), but the specific mechanism of virus reactivation and latency remains unknown. It was proposed that circulating microRNAs (miRNAs) in body fluids could potentially indicate infection. However, the connection between herpes zoster and circulating miRNAs has not been demonstrated. In this study, 41 HZ patients without superinfection were selected. The serum miRNA levels were analyzed by TaqMan low density array (TLDA) and confirmed individually by quantitative reverse transcription PCR (RT-qPCR) analysis. Thirty-five age-matched subjects without any infectious diseases or inflammation were selected as controls. The results showed that the serum miRNA expression profiles in 41 HZ patients were different from those of control subjects. Specifically, 18 miRNAs were up-regulated and 126 were down-regulated more than two-fold in HZ patients compared with controls. The subsequent confirmation of these results by qRT-PCR, as well as receiver operating characteristic (ROC) curve analysis, revealed that six kinds of miRNAs, including miR-190b, miR-571, miR-1276, miR-1303, miR-943, and miR-661, exhibited statistically significant enhanced expression levels (more than four-fold) in HZ patients, compared with those of healthy controls and herpes simplex virus (HSV) patients. Subsequently, it is proposed that these circulating miRNAs are capable of regulating numerous pathways and some may even participate in the inflammatory response or nervous system activity. This study has initially demonstrated that the serum miRNA expression profiles in HZ patients were different from those of uninfected individuals. Additionally, these findings also suggest that six of the altered miRNA could be potentially used as biomarkers to test for latent HZ infection.<\/jats:p>","DOI":"10.3390\/v8120326","type":"journal-article","created":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T10:36:37Z","timestamp":1480674997000},"page":"326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of microRNA Expression in Patients with Herpes Zoster"],"prefix":"10.3390","volume":"8","author":[{"given":"Xihan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Epidemiology, School of Public Health, The Key Laboratory of Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pain, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing 210008, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yucheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, The Key Laboratory of Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"He","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health, The Key Laboratory of Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.ijid.2007.08.002","article-title":"Incidence of herpes zoster and seroprevalence of varicella-zoster virus in young adults of South Korea","volume":"12","author":"Kang","year":"2008","journal-title":"Int. J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1586\/erv.10.30","article-title":"Herpes zoster and postherpetic neuralgia","volume":"9","author":"Johnson","year":"2010","journal-title":"Expert Rev. Vaccines"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"274","DOI":"10.4065\/84.3.274","article-title":"Herpes zoster (shingles) and postherpetic neuralgia","volume":"84","author":"Sampathkumar","year":"2009","journal-title":"Mayo Clin. Proc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1007\/s40266-012-0014-3","article-title":"Diagnosing and managing postherpetic neuralgia","volume":"29","author":"Nalamachu","year":"2012","journal-title":"Drugs Aging"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zajkowska, A., Garkowski, A., Swierzbinska, R., Kulakowska, A., Krol, M.E., Ptaszynska-Sarosiek, I., Nowicka-Cieluszecka, A., Pancewicz, S., Czupryna, P., and Moniuszko, A. (2016). Evaluation of Chosen Cytokine Levels among Patients with Herpes Zoster as Ability to Provide Immune Response. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0150301"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1056\/NEJMoa051016","article-title":"A vaccine to prevent herpes zoster and postherpetic neuralgia in older adults","volume":"352","author":"Oxman","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1742-4682-9-21","article-title":"Antiviral activity of salivary microRNAs for ophthalmic herpes zoster","volume":"9","author":"Irmak","year":"2012","journal-title":"Theor. Biol. Med. Model."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.devcel.2014.12.013","article-title":"A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis","volume":"32","author":"Ristori","year":"2015","journal-title":"Dev. Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1038\/gt.2011.50","article-title":"MicroRNA therapeutics","volume":"18","author":"Broderick","year":"2011","journal-title":"Gene Ther."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Hennessey, P.T., Sanford, T., Choudhary, A., Mydlarz, W.W., Brown, D., Adai, A.T., Ochs, M.F., Ahrendt, S.A., Mambo, E., and Califano, J.A. (2012). Serum microRNA biomarkers for detection of non-small cell lung cancer. PLoS ONE, 7.","DOI":"10.1371\/annotation\/bda2b1d8-1054-481a-bafd-2bcfa48514e6"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Sun, Y., Wang, M., Lin, G., Sun, S., Li, X., Qi, J., and Li, J. (2012). Serum microRNA-155 as a potential biomarker to track disease in breast cancer. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0047003"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1161\/CIRCRESAHA.110.226357","article-title":"Plasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes","volume":"107","author":"Zampetaki","year":"2010","journal-title":"Circ. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/j.1601-0825.2009.01604.x","article-title":"Exosomes from human saliva as a source of microRNA biomarkers","volume":"16","author":"Michael","year":"2010","journal-title":"Oral Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"D381","DOI":"10.1093\/nar\/gkl1009","article-title":"ViTa: Prediction of host microRNAs targets on viruses","volume":"35","author":"Hsu","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.3390\/v6041823","article-title":"Dysregulated microRNA expression in serum of non-vaccinated children with varicella","volume":"6","author":"Qi","year":"2014","journal-title":"Viruses"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.bbagrm.2008.05.005","article-title":"The roles of microRNAs in mammalian virus infection","volume":"1779","author":"Grassmann","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/ni.2073","article-title":"The microRNA miR-29 controls innate and adaptive immune responses to intracellular bacterial infection by targeting interferon-gamma","volume":"12","author":"Ma","year":"2011","journal-title":"Nat. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cizeron-Clairac, G., Lallemand, F., Vacher, S., Lidereau, R., Bieche, I., and Callens, C. (2015). MiR-190b, the highest up-regulated miRNA in ERalpha-positive compared to ERalpha-negative breast tumors, a new biomarker in breast cancers?. BMC Cancer, 15.","DOI":"10.1186\/s12885-015-1505-5"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Au, K.Y., Pong, J.C., Ling, W.L., and Li, J.C. (2016). MiR-1303 Regulates Mycobacteria Induced Autophagy by Targeting Atg2B. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146770"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"598","DOI":"10.7150\/jca.8052","article-title":"Newly Identified Cancer-Associated Role of Human Neuronal Growth Regulator 1 (NEGR1)","volume":"5","author":"Kim","year":"2014","journal-title":"J. Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"W169","DOI":"10.1093\/nar\/gkm415","article-title":"DAVID Bioinformatics Resources: Expanded annotation database and novel algorithms to better extract biology from large gene lists","volume":"35","author":"Huang","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"27931","DOI":"10.1038\/srep27931","article-title":"Transcriptome sequencing uncovers a three-long noncoding RNA signature in predicting breast cancer survival","volume":"6","author":"Guo","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.1128\/JVI.00189-16","article-title":"Longitudinal Examination of the Intestinal Lamina Propria Cellular Compartment of Simian Immunodeficiency Virus-Infected Rhesus Macaques Provides Broader and Deeper Insights into the Link between Aberrant MicroRNA Expression and Persistent Immune Activation","volume":"90","author":"Kumar","year":"2016","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.breast.2016.05.007","article-title":"rs11895168 C allele and the increased risk of breast cancer in Isfahan population","volume":"28","author":"Salimi","year":"2016","journal-title":"Breast"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.neuro.2015.08.001","article-title":"Propofol-induced rno-miR-665 targets BCL2L1 and influences apoptosis in rodent developing hippocampal astrocytes","volume":"51","author":"Sun","year":"2015","journal-title":"Neurotoxicology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1002\/stem.1640","article-title":"MicroRNA screen of human embryonic stem cell differentiation reveals miR-105 as an enhancer of megakaryopoiesis from adult CD34+ cells","volume":"32","author":"Kamat","year":"2014","journal-title":"Stem Cells"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Rawlings-Goss, R.A., Campbell, M.C., and Tishkoff, S.A. (2014). Global population-specific variation in miRNA associated with cancer risk and clinical biomarkers. BMC Med. Genom., 7.","DOI":"10.1186\/1755-8794-7-53"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"20032","DOI":"10.1038\/srep20032","article-title":"Increased serum microRNAs are closely associated with the presence of microvascular complications in type 2 diabetes mellitus","volume":"6","author":"Wang","year":"2016","journal-title":"Sci. Rep."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/8\/12\/326\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:27:53Z","timestamp":1760210873000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/8\/12\/326"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,2]]},"references-count":29,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2016,12]]}},"alternative-id":["v8120326"],"URL":"https:\/\/doi.org\/10.3390\/v8120326","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,2]]}}}