{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:34:45Z","timestamp":1768260885298,"version":"3.49.0"},"reference-count":50,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:00:00Z","timestamp":1500940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The Chinese giant salamander iridovirus (CGSIV), belonging to the genus Ranavirus in the family Iridoviridae, is the causative agent of an emerging infectious disease causing high mortality of more than 90% and economic losses in Chinese giant salamanders in China. In this study, a recombinant baculovirus-based vaccine expressing the CGSIV major capsid protein (MCP) was developed and its protective immunity in Chinese giant salamanders was evaluated. The recombinant Autographa californica nucleopolyhedrosis virus (AcNPV), expressing CGSIV MCP, designated as AcNPV-MCP, was generated with the highest titers of 1 \u00d7 108 plaque forming units\/mL (PFU\/mL) and confirmed by Western blot and indirect immunofluorescence (IIF) assays. Western blot analysis revealed that the expressed MCP reacted with mouse anti-MCP monoclonal antibodies at the band of about 53 kDa. The results of IIF indicated that the MCP was expressed in the infected Spodoptera frugiperda 9 (Sf9) cells with the recombinant baculovirus, and the Chinese giant salamander muscle cells also transduced with the AcNPV-MCP. Immunization with the recombinant baculovirus of AcNPV-MCP elicited robust specific humoral immune responses detected by ELISA and neutralization assays and potent cellular immune responses in Chinese giant salamanders. Importantly, the effective immunization conferred highly protective immunity for Chinese giant salamanders against CGSIV challenge and produced a relative percent of survival rate of 84%. Thus, the recombinant baculovirus expressing CGSIV MCP can induce significant immune responses involving both humoral and cell-mediated immunity in Chinese giant salamanders and might represent a potential baculovirus based vaccine candidate for Chinese giant salamanders against CGSIV.<\/jats:p>","DOI":"10.3390\/v9080195","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T10:04:36Z","timestamp":1500977076000},"page":"195","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Vaccination with Recombinant Baculovirus Expressing Ranavirus Major Capsid Protein Induces Protective Immunity in Chinese Giant Salamander, Andrias davidianus"],"prefix":"10.3390","volume":"9","author":[{"given":"Xiaoyuan","family":"Zhou","sequence":"first","affiliation":[{"name":"Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 2 Fenghui Road, South of Sanqiao, District of Weiyang, Xi\u2019an 710086, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinglang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 2 Fenghui Road, South of Sanqiao, District of Weiyang, Xi\u2019an 710086, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yahui","family":"Han","sequence":"additional","affiliation":[{"name":"Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 2 Fenghui Road, South of Sanqiao, District of Weiyang, Xi\u2019an 710086, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuhong","family":"Jia","sequence":"additional","affiliation":[{"name":"Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 2 Fenghui Road, South of Sanqiao, District of Weiyang, Xi\u2019an 710086, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwei","family":"Gao","sequence":"additional","affiliation":[{"name":"Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 2 Fenghui Road, South of Sanqiao, District of Weiyang, Xi\u2019an 710086, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gray, M.J., and Chinchar, V.G. (2015). Distribution and host range of ranaviruses. Ranaviruses: Lethal Pathogens of Ectothermic Vertebrates, Springer.","DOI":"10.1007\/978-3-319-13755-1"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jcpa.2010.11.012","article-title":"First report of a ranavirus associated with morbidity and mortality in farmed Chinese giant salamanders (Andrias davidianus)","volume":"145","author":"Geng","year":"2010","journal-title":"J. Comp. Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.3201\/eid1712.101758","article-title":"Iridovirus infection in Chinese giant salamanders, China, 2010","volume":"17","author":"Dong","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.vetmic.2014.05.009","article-title":"Virion-associated viral proteins of a Chinese giant salamander (Andrias davidianus) iridovirus (genus Ranavirus) and functional study of the major capsid protein (MCP)","volume":"172","author":"Li","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1007\/s00705-013-1962-6","article-title":"Pathological and microbiological findings from mortality of the Chinese giant salamander (Andrias davidianus)","volume":"159","author":"Meng","year":"2014","journal-title":"Arch. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1007\/s11427-015-4802-y","article-title":"Virus genomes and virus-host interactions in aquaculture animals","volume":"58","author":"Zhang","year":"2015","journal-title":"Sci. China Life Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.vetmic.2014.10.028","article-title":"The immunological responses and protection in Chinese giant salamander, Andrias davidianus, induced by the inactivated iridovirus","volume":"174","author":"Liu","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jviromet.2015.07.009","article-title":"Development of an immunochromatography assay kit for rapid detection of ranavirus","volume":"223","author":"Kim","year":"2014","journal-title":"J. Virol. Methods"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"28647","DOI":"10.3390\/ijms161226118","article-title":"Cloning of the major capsid protein (MCP) of grouper iridovirus of Taiwan (TGIV) and preliminary evaluation of a recombinant MCP vaccine against TGIV","volume":"16","author":"Liu","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5662","DOI":"10.1016\/j.vaccine.2015.08.054","article-title":"Protective immunity of a Pichia pastoris expressed recombinant iridovirus major capsid protein in the Chinese giant salamander, Andrias davidianus","volume":"33","author":"Zhou","year":"2015","journal-title":"Vaccine"},{"key":"ref_11","first-page":"1055","article-title":"Construction and immune efficacy of an MCP-containing DNA vaccine for Chinese giant salamander iridovirus","volume":"22","author":"Zeng","year":"2015","journal-title":"J. Fish. Sci. China"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1099\/vir.0.19688-0","article-title":"Heterologous expression of the modified coat protein of Cowpea chlorotic mottle bromovirus results in the assembly of protein cages with altered architectures and function","volume":"85","author":"Brumfild","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13337-013-0186-4","article-title":"Viral vaccines for farmed finfish","volume":"25","author":"Dhar","year":"2014","journal-title":"Virus Disease"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/BF00350390","article-title":"Baculovirus-insect cell interactions","volume":"20","author":"Blissard","year":"1996","journal-title":"Cytotechnology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jbiotec.2007.05.022","article-title":"Post-transcriptional regulatory element boosts baculovirus-mediated gene expression in vertebrate cells","volume":"131","author":"Mahonen","year":"2007","journal-title":"J. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.virol.2005.06.006","article-title":"Immunization against influenza A virus: Comparison of conventional in activated, live-attenuated and recombinant baculovirus produced purified hemagglutinin and neuraminidase vaccines in a murine model system","volume":"339","author":"Brett","year":"2005","journal-title":"Virology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/sj.mt.6300008","article-title":"Baculovirus-based vaccination vectors allow for efficient induction of immune responses against Plasmodium falciparum circumsporozoite protein","volume":"15","author":"Strauss","year":"2007","journal-title":"Mol. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.virol.2009.06.017","article-title":"Localization of VP28 on the baculovirus envelope and its immunogenicity against white spot syndrome virus in Penaeus monodon","volume":"391","author":"Madhan","year":"2009","journal-title":"Virology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty percent end points","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_20","unstructured":"O\u2019Reilly, D., Miller, L.K., and Luckow, V.A. (1992). Baculovirus Expression Vectors: A Laboratory Manual, Freeman and Company."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4839","DOI":"10.3390\/v6124839","article-title":"Virus-like particles of chimeric recombinant porcine circovirus type 2 as antigen vehicle carrying foreign epitopes","volume":"6","author":"Zhang","year":"2014","journal-title":"Viruses"},{"key":"ref_22","first-page":"1923","article-title":"Preparation and preliminary application of a monoclonal antibody against MCP COE protein of Chinese giant salamander (Andrias davidianus) iridovirus","volume":"40","author":"Zhou","year":"2016","journal-title":"Shui Chan Xue Bao"},{"key":"ref_23","unstructured":"Zhou, X.Y., Zhang, X.L., Jia, Q.H., Han, Y.H., and Zhang, H. (2017). Establishment and Application of Serological Detection Method for Antibody against Chinese Giant Salamander (Andrias davidianus) Iridoviride, Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences. Unpublished work."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3370","DOI":"10.12659\/MSM.900380","article-title":"Virus-like particle produced in Pichia pastoris induce protective immune responses against Coxsackievirus A16 in mice","volume":"22","author":"Feng","year":"2016","journal-title":"Med. Sci. Monit."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394Ct","volume":"25","author":"Livak","year":"2001","journal-title":"Method"},{"key":"ref_26","first-page":"447","article-title":"Potency testing of fish vaccines. International Symposium on Fish Biologics: Serodiagnostics and Vaccines","volume":"49","author":"Amend","year":"1981","journal-title":"Dev. Biol. Stand."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.virol.2006.07.009","article-title":"Inhibition of iridovirus protein synthesis and virus replication by antisense morpholino oligonucleotides targeted to the major capsid protein, the 18 kDa immediate-early protein, and a viral homolog of RNA polymerase II","volume":"358","author":"Sample","year":"2007","journal-title":"Virology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/j.dci.2010.12.002","article-title":"Antisense approaches for elucidating ranavirus gene function in an infected fish cell line","volume":"35","author":"Whitley","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.fsi.2005.10.012","article-title":"Genetic vaccines protect red seabream, Pagrus major, upon challenge with red seabream iridovirus (RSIV)","volume":"21","author":"Caipang","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9799","DOI":"10.1128\/JVI.77.18.9799-9808.2003","article-title":"In vitro and in vivo gene delivery by recombinant baculovimses","volume":"77","author":"Tani","year":"2003","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/1743-422X-8-77","article-title":"A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice","volume":"8","author":"Cao","year":"2011","journal-title":"Virol. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1016\/j.vaccine.2009.11.044","article-title":"Development of a novel viral DNA vaccine against human papillomavirus: AcHERV-HP16L1","volume":"28","author":"Lee","year":"2010","journal-title":"Vaccine"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.virol.2012.07.001","article-title":"Susceptibility of Xenopus laevis tadpoles to infection by the ranavirus Frog-Virus 3 correlates with a reduced and delayed innate immune response in comparison with adult frogs","volume":"432","author":"Chen","year":"2012","journal-title":"Virology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.dci.2005.09.007","article-title":"Generation of a long-lasting, protective, and neutralizing antibody response to the ranavirus FV3 by the frog Xenopus","volume":"30","author":"Maniero","year":"2006","journal-title":"Dev. Comp. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.dci.2012.07.004","article-title":"Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides","volume":"38","author":"Wang","year":"2012","journal-title":"Dev. Comp. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jcpa.2007.08.003","article-title":"Immunohistochemical demonstration of ranavirus antigen in the tissues of infected frogs (Rana temporaria) with systemic hemorrhagic or cutaneous ulcerative disease","volume":"138","author":"Cunningham","year":"2008","journal-title":"J. Comp. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3354\/dao02868","article-title":"Characterization of Chinese giant salamander iridovirus tissue tropism and inflammatory response after infection","volume":"114","author":"Jiang","year":"2015","journal-title":"Dis. Aquat. Org."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.fsi.2005.01.010","article-title":"The interferon system of teleost fish","volume":"20","author":"Robertsen","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"R251","DOI":"10.1186\/gb-2007-8-11-r251","article-title":"Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish","volume":"8","author":"Stein","year":"2007","journal-title":"Genome Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5766","DOI":"10.1128\/JVI.00223-14","article-title":"The amphibian (Xenopus laevis) type I interferon response to frog virus 3: New insight into ranavirus pathogenicity","volume":"88","author":"Grayfer","year":"2014","journal-title":"J. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.molimm.2015.02.015","article-title":"Identification of type I IFN in Chinese giant salamander (Andrias davidianus) and the response to an iridovirus infection","volume":"65","author":"Chen","year":"2015","journal-title":"Mol. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF01379125","article-title":"Induction of interferon by virus glycoprotein(s) in lymphoid cells through interaction with the cellular receptors via lectin-like action: An alternative interferon induction mechanism","volume":"138","author":"Ito","year":"1994","journal-title":"Arch. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7048","DOI":"10.1128\/JVI.74.15.7048-7054.2000","article-title":"DNA vaccines encoding viral glycoproteins induce nonspecific immunity and Mx protein synthesis in fish","volume":"74","author":"Leong","year":"2000","journal-title":"J. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1006\/fsim.2002.0422","article-title":"DNA vaccines as a tool for analysing the protective immune response against rhabdoviruses in rainbow trout","volume":"12","author":"Lorenzen","year":"2002","journal-title":"Fish Shellfish Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.vetimm.2012.05.021","article-title":"Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination","volume":"149","author":"Wang","year":"2012","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.dci.2012.06.003","article-title":"Molecular regulation of interferon antiviral response in fish","volume":"38","author":"Zhang","year":"2012","journal-title":"Dev. Comp. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1128\/JVI.79.5.2847-2858.2005","article-title":"Involvement of the Toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus","volume":"79","author":"Abe","year":"2005","journal-title":"J. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4912","DOI":"10.1128\/JVI.02486-09","article-title":"Innate immune responses and permissiveness to ranavirus infection of peritoneal leukocytes in the frog Xenopus laevis","volume":"84","author":"Morales","year":"2010","journal-title":"J. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2217\/17460794.3.1.35","article-title":"Baculovirus vector for gene delivery and vaccine development","volume":"3","author":"Tani","year":"2008","journal-title":"Future Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.4049\/jimmunol.171.3.1133","article-title":"Baculovirus induces an innate immune response and confers protection from lethal influenza virus infection in mice","volume":"171","author":"Abe","year":"2003","journal-title":"J. Immunol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/9\/8\/195\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:43:55Z","timestamp":1760208235000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/9\/8\/195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,25]]},"references-count":50,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2017,8]]}},"alternative-id":["v9080195"],"URL":"https:\/\/doi.org\/10.3390\/v9080195","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,25]]}}}