{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T19:35:50Z","timestamp":1772048150258,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T00:00:00Z","timestamp":1505433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019018","name":"CAMS Initiative for Innovative Medicine","doi-asserted-by":"publisher","award":["2016-I2M-1-019"],"award-info":[{"award-number":["2016-I2M-1-019"]}],"id":[{"id":"10.13039\/501100019018","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Technology Plan Project of Yunnan Province","award":["2014BC008"],"award-info":[{"award-number":["2014BC008"]}]},{"name":"Technology Plan Project of Yunnan Province","award":["2016FB034"],"award-info":[{"award-number":["2016FB034"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal\u2013oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459\u2013606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.<\/jats:p>","DOI":"10.3390\/v9090260","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T10:22:10Z","timestamp":1505470930000},"page":"260","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice"],"prefix":"10.3390","volume":"9","author":[{"given":"Kui","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China"},{"name":"Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming 650101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri","family":"Kusov","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of L\u00fcbeck, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"},{"name":"German Center for Infection Research (DZIF), Hamburg-L\u00fcbeck-Borstel-Riems Site, University of L\u00fcbeck, 23562 L\u00fcbeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guan","family":"Ying","sequence":"additional","affiliation":[{"name":"China Tobacco Yunnan Industrial Co., Ltd., 41 Keyi Road, Kunming 650106, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming 650101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Jinyuan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Na","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Hongjun","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun","family":"Maosheng","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1093\/clind\/16.5.612","article-title":"Summary of current nomenclature, taxonomy, and classification of various microbial agents. 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